The genetic diversity and relationship of 10 Dendrobium germplasm resources were analyzed by RAPD
The genomic DNA of 10 Dendrobium germplasms were tested with RAPD using 17 arbitrary 10-mer primers which were screened from 100 primers. The 200 polymorphic bands ofRAPD bands were obtained.The average of polymorphic bands of per primer was 11.8. The RAPD based genetic similarity ranged from 0.356 to 0.676. UPGMA cluster analysis based on RAPD molecular markers data divided 10 species into four groups. The results showed that the RAPD approach was more efficient on Study of genetic background and relationship of Dendrobium germplasms .
Ren Yu, Yang Guangsui, Yin Junmei, Zhan Yanling, Huang Xian.
Analysis of Genetic Diversity in Dendrobium Germplasm by RAPD Makers. Chinese Agricultural Science Bulletin. 2007, 23(6): 598-598 https://doi.org/10.11924/j.issn.1000-6850.0706598
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1]徐雨,王四清.异军突起之石斛兰的研究进展.华北农学报,2005,20(专辑):152-157. [2]赵天榜,陈志秀,陈占宽,等.石斛组织培养与栽培技术的研究[J].河南农业大学学报,1994,28(2):128-130. [3]邢福桑,冯锦东,刘凌峰,等.铁皮石斛栽培技术的研究概况[J].时珍国医国药,2002,13(9):559. [4]曾宋君,程式君,张京丽,等.五种石斛兰的胚培养及其快速繁殖研究[J].园艺学报,1998,25(1):75- 80. [5]罗岚,关仕港,刘建昌,等.秋石斛离体快速繁殖研究[J].佛山科学技术学院学报(自然科学版),2004,22(2): 69-71. [6]查学强,罗建平.霍山石斛原球茎液体培养的营养调节[J].合肥工业大学学报,2004,(1):53-57. [7]张玲,张治国.铁皮石斛种子试管苗适宜培养基研究[J].浙江省医学科学院学报,1997,8(1):4-6. [8]马国祥,徐国钧,徐珞珊,等.鼓槌石斛及其化学成分的抗肿瘤活性作用[J].中国药科大学学报,1994,25(3) : 188-189. [9]毕志明,王峥涛,徐珞珊,等.流苏石斛化学成分研究[J].药学学报,2003,38(7):526-529. [10]杨虹,龚燕晴,王峥涛,等.鼓槌石斛化学成分的研究[J].中草药,2001,32(11):972-974. [11]郑卫平,唐于平,楼凤昌,等. 迭鞘石斛的化学成分研究[J].中国药科大学学报,2000,31(1):5-7. [12]王玉东.兰属和石斛属植物遗传多样性的研究:[学位论文].浙江:浙江工业大学,2004. [13]Yue G H, Lam-chan L T. et al. Development of simple sequence repeat (SSR) markers and their use in identification of Dendrobium varieties Molecular Ecology Notes 2006, 6:832-834 [14]顾红雅,瞿礼嘉,明小天,等.植物基因与分子操作.北京:北京大学出版社,1995:19-25. [15]吉占和.中国植物志(第十九卷).北京:科学出版社,1999:67-146.
