烟草感染青枯菌前后POD及同工酶的变化

胡青平,,徐建国,薄芳芳,陈五岭

中国农学通报. 2007, 23(6): 497-497

中国农学通报 ›› 2007, Vol. 23 ›› Issue (6) : 497-497. DOI: 10.11924/j.issn.1000-6850.0706497
植物保护 农药

烟草感染青枯菌前后POD及同工酶的变化

  • 胡青平,,徐建国,薄芳芳,陈五岭
作者信息 +

Changes of Peroxidase and Isoenzyme in Tobacco Infected by Pseudomonas Solanacearum

  • Hu Qingping,, Xu Jianguo, Bo Fangfang, Chen Wuling
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摘要

【研究目的】对烟草感染青枯菌前后的根、茎、叶内过氧化物酶（POD）及同工酶进行比较研究，为抗病育种提供理论基础。【方法】采用比色法测POD酶活，用聚丙烯酰胺凝胶电泳（PAGE）进行POD同工酶的比较。【结果】对烟草感染青枯菌前后，酶活性测定结果表明，烟草叶部POD活性显著高于根部和茎部。烟草接菌后,根、茎、叶内的POD活性均在第3天升至最高,而叶内比对照增高了29.67%。同工酶电泳分析表明，烟草接菌后的同工酶谱带，在条带的数目、种类和各条的相对活性上表现出差异。【结论】POD作为植物的保护酶类，随植物抵抗疾病的过程变化而变化，故与植物抗病性密切相关

Abstract

【OBJECTIVE】 Comparatively peroxidase (POD) and isoenzyme of roots, stems and leaves in tobacco infected with Pseudomonas solanacearum were studied in order to offer academic foundations for breeding resistive plants. 【METHOD】 Colorimetry for POD activity and polypropylene amic gel electrophoresis (PAGE) for isoenzyme were used. 【RESULTS】 After inoculated by Pseudomonas Solanacearum, it was found that there was higher peroxidase (POD) activity in leaves than that in roots and in stems, and the POD activity in roots, stems and leaves was highest in 3 days but in leaves which improved 29.67% percent than those form the contrast. Isoenzyme electrophoresis showed that difference occurred in numbers and types of isoenzyme bands and related activity of every band between different samples. 【CONCLUSION】 POD was a protectal enzyme and it was changing with infection, so it was suggested that POD may have close relation to resistance against disease.

关键词

烟草；青枯病；POD；同工酶

Key words

Tobacco;Pseudomonas Solanacearum;Peroxidase;Isoenzyme

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胡青平,,徐建国,薄芳芳,陈五岭. 烟草感染青枯菌前后POD及同工酶的变化. 中国农学通报. 2007, 23(6): 497-497 https://doi.org/10.11924/j.issn.1000-6850.0706497
Hu Qingping,, Xu Jianguo, Bo Fangfang, Chen Wuling. Changes of Peroxidase and Isoenzyme in Tobacco Infected by Pseudomonas Solanacearum. Chinese Agricultural Science Bulletin. 2007, 23(6): 497-497 https://doi.org/10.11924/j.issn.1000-6850.0706497

参考文献

[1]胡青平,徐建国,陈五岭.番茄茎内生细菌的分离鉴定及青枯病拮抗菌的筛选.西北植物学报,2005,26(10):2039-2043.
[2]蒋选利,李振岐.康振生.过氧化物酶与植物抗病性研究进展.西北农林科技大学学报,2001,29(6):124-129.
[3]潘建菁,纪成灿,刘冬霞.青枯菌侵染后烟株体内过氧化物酶活性的变化及其与抗病性的关系.中国烟草科学,2004,(3):28-30.
[4]宋从凤,王金生,施仲美.桉树对青枯病抗性与过氧化物酶及同工酶关系的研究.广西林业科学,2000,29(1):7-10.
[5]徐金星,刘忠民,刘翠明.大豆品种接种SMV叶片POD同工酶的研究.东北农业大学学报,2000,31(1):20-25.
[6]Ray H, Hammerschnidt R. Responses of potato tuber to infection by Fusarium sambucinum .Physiological and Molecular Plant Pathology,1998,53(2):81-92.
[7]VitaliA,BottaB,MonacheG D,et al. Purification and partial characterization of a peroxidase from plant cell cultures of cassia didymobotrya and biotransformation studies. Biochemical Journal London,1998,331(2):513-519.
[8]YubedeeA G. Role of polyphenol oxidase, peroxidase and total phenol content in differential resistance of Dioscorea species to Fusarium moniliforme. Indian Journal of Agricultural Sciences,1998, 68(10):644-646.
[9]徐建华,利容干.黄瓜不同抗病品种感染镰刀菌枯萎病菌后几种酶活性的变化.植物病理学报,1995,25(3):239-242.

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