An ultrasensitive time-resolved fluorescent immunoassay method for determination aflatoxins B_1 in edible oil

Du Wang, Zhaowei Zhang, Peiwu Li, Qi Zhang, Jin Mao, Li Yu, Wen Zhang, Xiaoxia Ding

油料作物学报(英文版). 2016, 1(03): 57-64

油料作物学报(英文版) ›› 2016, Vol. 1 ›› Issue (03) : 57-64. DOI: 10.1016/j.ocsci.2016-0022

An ultrasensitive time-resolved fluorescent immunoassay method for determination aflatoxins B_1 in edible oil

  • Du Wang, Zhaowei Zhang, Peiwu Li, Qi Zhang, Jin Mao, Li Yu, Wen Zhang, Xiaoxia Ding
作者信息 +

An ultrasensitive time-resolved fluorescent immunoassay method for determination aflatoxins B_1 in edible oil

  • Du Wang, Zhaowei Zhang, Peiwu Li, Qi Zhang, Jin Mao, Li Yu, Wen Zhang, Xiaoxia Ding
Author information +
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摘要

Edible oil is one major nutritional ingredient to human and widely consumed directly.The contamination of aflatoxin B_1 in edible oils has been attracted extensive efforts due to its hazard to human health and life.To avoid the digestion of edible oils contaminated by AFB_1,the development of rapid and sensitive sensing method for AFB_1 is required.Herein,a quantitative,sensitive and rapid method for AFB_1 detection in edible oils was proposed by using ultrasensitive time-resolved fluorescent immunosensing(TRFIS)method.This method poses unique advantages from both time-resolved fluorescent sensing method and immunochromatographic assay format.The nanospheres were modified with fluorescent europium and then captured the home-made monoclonal antibody against AFB_1(3G1).After optimization,by using a competitive immunosensing manner,this TRFIS method has a detectable linear range of 0.54-20.0μg/kg with minimum detectable concentration of 0.18μg/kg.It can be completed merely within 10 min with recovery from 87.0%to 121.9%.The agreement was observed between the results by TRFIS and high performance liquid chromatography(HPLC)methods.This research provides a promising sensing method for sensitive and rapid determining AFB_1 in edible oils.

Abstract

Edible oil is one major nutritional ingredient to human and widely consumed directly.The contamination of aflatoxin B_1 in edible oils has been attracted extensive efforts due to its hazard to human health and life.To avoid the digestion of edible oils contaminated by AFB_1,the development of rapid and sensitive sensing method for AFB_1 is required.Herein,a quantitative,sensitive and rapid method for AFB_1 detection in edible oils was proposed by using ultrasensitive time-resolved fluorescent immunosensing(TRFIS)method.This method poses unique advantages from both time-resolved fluorescent sensing method and immunochromatographic assay format.The nanospheres were modified with fluorescent europium and then captured the home-made monoclonal antibody against AFB_1(3G1).After optimization,by using a competitive immunosensing manner,this TRFIS method has a detectable linear range of 0.54-20.0μg/kg with minimum detectable concentration of 0.18μg/kg.It can be completed merely within 10 min with recovery from 87.0%to 121.9%.The agreement was observed between the results by TRFIS and high performance liquid chromatography(HPLC)methods.This research provides a promising sensing method for sensitive and rapid determining AFB_1 in edible oils.

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Du Wang, Zhaowei Zhang, Peiwu Li, Qi Zhang, Jin Mao, Li Yu, Wen Zhang, Xiaoxia Ding. An ultrasensitive time-resolved fluorescent immunoassay method for determination aflatoxins B_1 in edible oil. 油料作物学报(英文版). 2016, 1(03): 57-64 https://doi.org/10.1016/j.ocsci.2016-0022
Du Wang, Zhaowei Zhang, Peiwu Li, Qi Zhang, Jin Mao, Li Yu, Wen Zhang, Xiaoxia Ding. An ultrasensitive time-resolved fluorescent immunoassay method for determination aflatoxins B_1 in edible oil. Oil Crop Science. 2016, 1(03): 57-64 https://doi.org/10.1016/j.ocsci.2016-0022

基金

supported by Special Fund for Grain-scientific Research in the Public Interest (201513006-02);; Special Fund for Agro-scientific Research in the Public Interest (201203094);; Natural Science Foundation of China (31401601)

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