利用高速逆流色谱技术制备灵芝酮三醇

岳亚文, 田振, 张劲松, 王晨光, 韩伟, 唐庆九, 冯娜, 周帅

食用菌学报. 2020, 27(02): 77-83

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食用菌学报 ›› 2020, Vol. 27 ›› Issue (02) : 77-83. DOI: 10.16488/j.cnki.1005-9873.2020.02.010
研究论文

利用高速逆流色谱技术制备灵芝酮三醇

  • 岳亚文1,2, 田振4, 张劲松1, 王晨光3, 韩伟2, 唐庆九1, 冯娜1*, 周帅1*
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Preparative Isolation of Ganodermanontriol from Ganoderma lucidum by Highspeed Countercurrent Chromatography

  • YUE Yawen, TIAN Zhen, ZHANG Jingsong, WANG Chenguang,HAN Wei, TANG Qingjiu, FENG Na, ZHOU Shuai
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摘要

以沪农灵芝(Ganoderma lucidum)1号子实体为原料,经过87%乙醇提取、D101大孔树脂富集、乙醇梯度洗脱获得弱极性三萜组分。采用高速逆流色谱技术,以正己烷∶乙酸乙酯∶甲醇∶水(V∶V∶V∶V=5∶6∶5∶5)为溶剂体系,对该三萜组分中灵芝酮三醇的制备工艺进行研究,在转速900 r·min-1、流速3 mL·min-1条件下,一次上样400 mg灵芝弱极性三萜组分,可得到纯度为91.6%的灵芝酮三醇26.8 mg。

Abstract

Ganodermanontriol was purified from fruiting bodies of Ganoderma lucidum (Hunong No.1) following a series of steps, including extraction by 87% ethanol, condensation by macroporous adsorption resin D101, gradient elution by ethanol to obtain the weak polar triterpenes fraction (WTF), and finally preparation of the target compound from WTF by high-speed countercurrent chromatography (HSCCC). For HSCCC, the solvent system was determined to be a mixture of N-hexane,ethyl acetate, methanol and water (V∶V∶V∶V=5∶6∶5∶5); the separation condition was set as rotation at 900 rpm and flow rate of 3.0 mL·min-1. Using the above mentioned HSCCC condition, a single loading amount of 400 mg WTF yielded 26.8 mg ganodermanontriol with a purity of 91.6%.

Key words

Ganoderma lucidum fruiting body; weak polar triterpenes; macroporous adsorption resin; high-speed countercurrent chromatography (HSCCC)

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岳亚文, 田振, 张劲松, 王晨光, 韩伟, 唐庆九, 冯娜, 周帅. 利用高速逆流色谱技术制备灵芝酮三醇. 食用菌学报. 2020, 27(02): 77-83 https://doi.org/10.16488/j.cnki.1005-9873.2020.02.010
YUE Yawen, TIAN Zhen, ZHANG Jingsong, WANG Chenguang, HAN Wei, TANG Qingjiu, FENG Na, ZHOU Shuai. Preparative Isolation of Ganodermanontriol from Ganoderma lucidum by Highspeed Countercurrent Chromatography. Acta Edulis Fungi. 2020, 27(02): 77-83 https://doi.org/10.16488/j.cnki.1005-9873.2020.02.010
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