为提高春兰品种间授粉坐果率、缩短种子无菌萌发时间、提高萌发率，对春兰品种自交和杂交的适宜授粉时间、种子无菌萌发进行研究。结果显示最佳授粉时间为花后2~5 天。品种自交坐果率仅33.3%，品种杂交的坐果率达100%。蒴果的纵横径生长呈单“S”形曲线。成熟种子萌发时间为281~296天；授粉后130 天的未成熟种子萌发时间仅需96~99 天，萌发数量最多，为116 ~132 个/瓶。本研究使春兰从授粉到种子萌发的时间缩短8个月，且萌发率增加，节约了成本，提高了效率。
Abstract
In order to improve pollination fruit- set rate, shorten seed germination time and improve seed germination rate of Cymbidium goeringii varieties, the authors studied the pollination time, fruit-set rate, seed aseptic germination of self-bred and hybridized Cymbidium goeringii varieties. The optimal pollination time was 2 to 5 days after flower blooming. Fruit-set rate of self-bred varieties was only 33.3%, and fruit-set rate of hybridized varieties reached 100%. The fruit vertical and horizontal diameter growth showed“S”shape curve. Mature seed germination time was 281-296 days, but the germination time of non-mature seed growing 130 days after pollination was the shortest, only 96-99 days, and the germination rate was the highest. The suitable time of capsules harvested was about 130 days after pollination. This test method would cut the time from pollination to seed germination by 8 months, increase germination rate, save the cost, and improve the efficiency.
关键词
春兰;种胚;无菌萌发
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Key words
Cymbidium goeringii; Seed; Aseptic Germination
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