为揭示中国中北部青檀的遗传多样性,本研究从贵州、北京、甘肃、江苏、安徽、山东、河南7省12个种群收集了31 份青檀样品,对其进行AFLP分析。利用从26 对AFLP引物组合中筛选出的6对引物对样本DNA进行扩增,共产生223条谱带,其中211 条为多态性条带,比率达94.26%。结果显示中国中北部的青檀材料之间存在较高的遗传多样性,且AFLP 技术可以有效地对其进行分析。31 个样品的遗传相似系数在0.6413-0.9193之间,平均为0.7489,聚类分析结果显示样品首先按照种群聚在一起,但也有交叉。
Abstract
In order to study the genetic diversity of Pteroceltis tatarinowii in the north of central China, 31 samples were collected from 12 populations from 7 provinces (Guizhou, Beijing, Gansu, Jiangsu, Anhui, Shandong and Henan) and AFLP analysis was conducted. A total of 223 bands were generated from 6 primer combinations screened from 26 primer combinations and 211 of those bands showed polymorphism, the polymorphic ratio (PP) was up to 94.26%. The results showed that a high genetic diversity was existed among Pteroceltis tatarinowii samples from the north of central China and AFLP analysis was effective. The genetic similarity coefficient was in the range of 0.6413 to 0.9193, with an average value of 0.7489. And the results of cluster analysis showed that the samples were clustered together according to the population, but there were intersections between the populations.
关键词
青檀;聚类分析;AFLP;遗传多样性
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Key words
Pteroceltis tatarinowii; Cluster Analysis; AFLP; Genetic Diversity
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