【研究目的】明确大赖草染色体对小麦农艺性状、抗病性和耐热性的效应,为进一步利用大赖草优异基因提供理论依据。【方法】通过黄矮病、白粉病、条锈病人工接种或自然发病鉴定抗病性,以分期播种模拟高温胁迫环境,通过千粒重变化评价耐热性,收获后调查农艺性状、再生性,对小麦-大赖草二体附加系、代换系和亲本‘中国春’进行农艺性状效应鉴定。【结果】结果表明,10个异染色体系苗期对白粉病菌株E09均表现高感,温室成株期白粉病抗性好于对照普通小麦‘中国春’;对条锈病抗性表现均优于‘中国春’。大赖草H染色体有增加穗长、小穗数、提早抽穗期、增加黄矮病抗性的正向效应。大赖草H、A染色体具有增加耐热性的正向效应;大赖草J染色体对粒长、粒宽和粒厚均有正向效应。大赖草A、L染色体含有控制芒的基因,大赖草F具有再生性和复小穗基因,【结论】10份异染色体系中未发现含高抗小麦白粉病基因的大赖草染色体,大赖草附加系DALr#A、DALr#F、DALr#H、DALr#J可用于小麦农艺性状、抗黄矮病、条锈病和耐热性的改良。
Abstract
【Objective】To explicit effect of chromosomes of Leymus racemosus on agronomic traits, disease resistance and heat tolerance in wheat would provide a theoretical basis for further utilization of excellent Genes.【Method】The resistances of barley yellow dwarf disease(BYDV), powdery mildew, stripe rust were identified by artificial inoculation or natural infection. InstallmentsSsowingSsimulationSenvironmentSof high temperature stress bySinterval sowingSunder the field,Sheat tolerance was evaluatedSbySwheat grain weightSchanges. Agronomic trait and regrowthSability of Chinese Spring- L. racemosus addition lines and substitution line and their wheat background Chinese Spring were investigated after harvest.【Result】The results showed that highly susceptible to powdery mildew to the E09 physiologicalSrace atSSeedling Stage, wereSbetter than lower that of Chinese Spring at adult stage in the greenhouses, and the alien chromosome lines with high yellow rust resistance than those from Chinese Spring. Positive effect of Chromosome H on spike length number of spikelet, early heading stage, increasing resistance to BYDV was found, Chromosome A, H on heat tolerance, Chromosomes J on grain size. Chromosome A, L containedSSSawn gene, Chromosome F with regrowth and supernumerary spikelet genes.【Conclusion】SWheat powdery mildew resistance genes were not found in L. racemosus chromosomes of the 10 Chinese Spring- L. racemosus alien chromosome lines. Addition line of DALr#A, DALr#F, DALr#H and DALr#J would be used in wheat breeding for improving agronomic trait, BYDV resistance,stripe rustSresistance and heat tolerance.
关键词
小麦;大赖草;异染色体系;农艺性状;抗病性;耐热性
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Key words
Wheat; Leymus racemosus; Alien Chromosome Lines; Agronomic Trait; Disease Resistance; Heat Tolerance
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