Pre-harvest sprouting (PHS) refers to the germination of cereal crops on the spike in high humidity conditions before grain harvest. Wheat PHS is a significant problem that affects both the yield and quality of wheat. Seed dormancy level is a major factor influencing the resistance of wheat PHS, and domesticated crops often exhibit reduced seed dormancy levels, making cultivated wheat more prone to PHS compared to its wild ancestors. Wheat PHS is mainly regulated by external environmental factors such as temperature and humidity, as well as internal plant hormones (GAs, ABA, IAA, MeJA, ET, BR). Researchers have identified a range of materials resistant to PHS, cloned key genes regulating PHS resistance, such as PM19, MFT, MKK3, Myb10-3D, Vp1. New wheat materials resistant to PHS have been successfully developed through molecular marker-assisted selection, artificial synthesis of wheat, and CRISPR/Cas9 gene editing technology. This article reviews the genetic mechanism of PHS resistance in wheat and the latest progress in PHS resistance breeding research. In the future, it is necessary to continue exploring key genes related to PHS resistance, and employ biotechnological breeding methods to cultivate new PHS-resistant wheat varieties.

The major objective of the present study is to show application potential of Helleborus in China and to accelerate development and utilization of Helleborus in the fields of medicine, manufacturing and landscaping. The biological characteristics, habits and cultivation techniques of the genus were reviewed, and the chemical components, medicinal effects, origin of names, ornamental characteristics and garden application were discussed. It was found that the subjects had significant medicinal, cultural, and ornamental value. It was pointed out that there were insufficient efforts in botany basic research, medicinal plant development, and new variety cultivation in current work. At the same time, countermeasures and suggestions were proposed accordingly.

Wheat stripe rust caused by Puccinia striiformis f. sp. tritici (Pst) is a devastating disease threaten food security in China and worldwide. Epidemics of wheat stripe rust have been under control through applying resistant cultivars and crop protection approaches. However, due to climate change, innovation of cropping system, improvement of breeding technology, yield level enhancement of wheat cultivars, variation in structure and frequency of virulence genes in Pst populations in the new era, the current status of stripe rust resistance genes in wheat breeding programs need to be evaluated. The results could provide useful information for applying stripe rust resistance genes to develop new wheat cultivars with broad-spectrum and durable rust resistance. After multiple year’s stripe rust resistance survey, genetic analysis, molecular tagging and mining of stripe rust resistance genes in wheat cultivars and advanced breeding lines, the current status of major stripe rust resistance genes utilization was reviewed. We summarized the present situations of major stripe rust resistance gene discovery and germplasm innovation, the most frequently used stripe rust resistance genes, new strategy for pyramiding adult plant partial resistance and all stage resistance, and molecular marker assisted selection for developing wheat cultivars with broad spectrum and durable resistance in China. This review also proposes the major research areas in wheat stripe rust resistance breeding in the new era.

Being effective only to the traits controlled by large-effect QTL,conventional marker-assisted selection(MAS)can hardly improve the quantitative traits that are controlled by many small-effect QTL.Later on, the genomic selection (GS) technology proposed in 2001 solves the problem of improving complex traits controlled by the minor polygenic effects due to its high prediction accuracy through estimating the breeding value of individual with high-density molecular markers. At present,it has been successfully applied to the genetic improvement of quantitative traits in animals and plants such as dairy cattle,pigs,sheep,maize,and wheat in the US,Canada,Australia,Germany,France and so on.As a breaking-through breeding technology and a hot spot in research and application now. In this review,we summarized the factors affecting predictive accuracy of GS and the research progress of GS in breeding maize,wheat,rice and rapeseed at home and abroad,and finally discussed the existing problems in its application. This review will provide some reference for GS of the crops in the future.

The key cultivation techniques of Passiflora edulis Sims were summarized and analyzed based on its production practice in the research area. During the planting period, site selection, soil preparation, bottom fertilization, scaffolding and seedling management were completed. During the growth period, the techniques of bud erasing, top picking and pruning were put forward, while also implementing timely fertilizer and water management as well as disease and pest control. Harvesting period, combined with different maturity periods for harvesting and storage preservation. The research results provided references for high-quality and high-yield cultivation techniques of Passiflora edulis Sims and the development of related industries.

With the continuous improvement in rice cultivation techniques, China has maintained a high rice production level of about 210 million tons over the past decade. Direct-seeding rice cultivation technology, recognized for its efficiency and simplicity, has been favored by Chinese farmers. However, controversies persist regarding direct-seeding rice compared to transplanted rice in national-scale production. Thus, this study employed meta-analysis techniques to quantify disparities in grain yield, economic benefit, rice quality, lodging characteristic, and greenhouse gas emissions between direct-seeding rice and transplanted rice. Our results indicated that direct-seeding rice significantly reduced grain yield by an average of 6.3% relative to transplanted rice, which was main due to the reduced total spikelet (-3.8%) and filled-grain percentage (-1.8%). In different planting systems in China, the yield of direct-seeding rice had significantly decreased compared to transplanted rice, and the direct-seeding rice-induced reductions in yield of single rice (-10.9%) and late rice (-13.1%) were higher than those of middle rice (-4.8%) and early rice (-4.4%). The grain yield reductions for direct-seeding rice were from 10% to 20% in Jilin, Liaoning, Xinjiang, Ningxia, Shandong, Jiangsu, and Zhejiang provinces, meanwhile Heilongjiang and Jiangxi provinces saw reductions of 5% to 10%, but it had no significant effect in other provinces. Direct-seeding rice resulted in comparable net economic return relative to transplanted rice (p> 0.05). Direct-seeding rice reduced milled rice rate (-3.1%) and gel consistency (-3.5%), improved appearance quality (chalkiness percentage and chalkiness degree, which decreased by 25.3% and 22.5%, respectively), whereas no significant effects were observed on nutrition quality and taste value. Direct-seeding rice increased lodging index at base of the first (+12.4%) and third (+10.3%) internodes, but not at the second internode, indicating an increase in risk of lodging relative to transplanted rice. In terms of greenhouse gas emissions, direct-seeding rice fields showed reductions in methane emissions (-42.8%), global warming potential (-36.2%), and greenhouse gas intensity (-41.1%) compared to transplanted rice fields, while promoting nitrous oxide emissions (+29.1%). In addition, a review was recounted on nitrogen utilization and its loss, water and energy use efficiency, and weed incidence. Finally, the recommendations for the future advancement of direct-seeding rice were proposed, main focusing on rice variety breeding, rice cultivation technique optimization, rice planting area layout, as well as policies and services with the goal of technological innovation and regionalized application of direct-seeding rice cultivation technology in China.

The research methods and recent progress of domestication breeding (systematic breeding), crossbreeding including single-single hybridization, single-double hybridization, multi-spore hybridization (self-crossing) and protoplast fusion breeding, mutation breeding and molecular breeding technologies of Auricularia cornea in China were summarized. Some newly bred cultivars and their characteristics were introduced, and the research methods for the propagation of A. cornea in solid and liquid strains were summed up. Molecular marker-assisted breeding, genome editing, genomic selection and other molecular breeding methods are proposed for future crossbreeding of A. cornea in China. The high-efficiency breeding technology will be rapidly applied, the commercial breeding of A. cornea will be actively promoted, and the ‘breeding- propagation- promotion’ system will be gradually improved.

This article studies the Chinese traditional medicinal and edible plant Citrus medica var. sarcodactylis (fingered citron), including its historical evolution from the Song Dynasty to the Qing Dynasty, the division of artificially cultivated varieties, and the change of planting bases. Base on the related literature review, we summarized the research progress of fingered citron cultivation technology and its medicinal and economic value. In addition, we pointed out the differences of Citrus medica var. sarcodactylis, Citrus medica var. medica and Citrus medica, from historical evolution, identifying that they were of the same family, same genus and different species. Finally, we raised the question of whether the differences between the cultivars from the main fingered citron producing areas were consistent with the National Pharmacopoeia, suggesting that regional standardization of cultivation technology should be established on variety, origin, soil, climate, etc.

Based on the production practice in the research area, the efficient cultivation and management technology system of Illicium verum forest from the aspects of grafting seedling (scion collection, rootstock selection and preparation, grafting methods, and seedling management), afforestation (forest selection, land preparation, density selection, and planting), young forest management, forest management (weeding, fertilization, reclamation, forest intercropping, and density control), pest control, and fruit harvesting and processing were explored and summarized in this article. The purpose was to increase the yield of Illicium verum forest and provided references for the sustainable development of related industries.

瓜蒌是药食两用的植物品种,不仅可以用于加工、生产食用瓜蒌籽,还可作为中药材的原料,其食用价值、药用价值和保健价值均较高。为发展瓜蒌特色产业,探究瓜蒌高产种植栽培技术要点,本文总结分析了瓜蒌高产栽培技术,以增加从业者收入,推动农业增产增效,为瓜蒌产业高质量发展提供参考。

The green, high-quality, and efficient cultivation techniques for tomatoes were summarized and analyzed. The technical points of tomatoes cultivation were mainly analyzed in detail from 4 aspects: tomatoes seed selection and seedling cultivation, transplanting and planting, field management, and prevention and control of major diseases and pests. Seed selection and seedling cultivation included site selection, land preparation and bed raising, sufficient base fertilizer application, and variety and seedling selection. Transplanting and planting included planting with pesticides, planting time and quantity, field management throughout the growth period of tomatoes, and prevention and control of major diseases and pests such as bacterial wilt, leaf mold, and stem rot. Tomato green, high-quality and efficient cultivation requires selecting high-quality varieties based on local conditions; Paid attention to proper pruning, adopt scientific fertilization methods, and supplement trace elements; Promoted roots and fruits in the early stage of tomatoes growth, and nourish roots and fruits in the middle and late stages; The prevention and control of diseases and pests required precise identification of the type of pest and disease, scientific application of pesticides, and active prevention. The results provided references for tomatoes cultivation.

Artemisia argyi is a perennial herb, and its leaves are used as medicine. It is a commonly used bulk Chinese medicinal herbal with high medicinal and economical value. The high-quality cultivation techniques of this plant were summarized and analyzed from 7 aspects of variety selection, land preparation, and propagation methods. Including select high-quality, high-yielding, and comprehensive resistant varieties of Artemisia argyi suitable for local cultivation; choose fertile loam and sandy loam environments with sufficient sunlight, good drainage and irrigation conditions, and deep plowing and fertilization before planting. Its propagation techniques include division propagation, root propagation, and cutting propagation; the suitable planting density is 97 500 to 120 000 plants/hm², and the appropriate amount of rhizomes for root propagation is 3 000 kg/hm². After planting, timely carry out standardized management work such as tillage, weeding, fertilization, irrigation, leveling, and thinning to promote plant growth; the prevention and control of pests and diseases in the field mainly rely on agricultural and physical methods, with chemical control as a supplement. Timely harvesting, processing and storage according to its application characteristics.It provides references for Artemisia argyi standardized production and high-quality industrial development.

Changliangyou Fuxiangzhan is a new variety of high yield and high quality indica hybrid rice. In 2022 and 2023, it was introduced into Dongxi Village, Mapo Town, Luchuan County, Yulin City, Guangxi for demonstration planting. This paper emphatically summarized the Changliangyou Fuxiangzhan demonstration of whole growth period, resistance, yield and ground rice quality performance, and from the good plot, dip bud, timely sowing, school-age throwing, formula fertilization, scientific pipe water, prevention and control of disease and ripe harvest introduced its high yield cultivation technology, for Changliangyou Fuxiangzhan a large area of production and application to provide reference.

旱优3015是上海市农业生物基因中心以节水抗旱稻优质三系不育系沪旱7A为母本,早熟矮秆强优势恢复系旱恢3015为父本配组而成的早熟高产籼型节水抗旱稻三系杂交组合,具有节水抗旱、早熟高产、株高矮、综合性状优和适应性广等优点,适合在长江中下游作一季稻种植。本文介绍了该组合的选育过程、组合特征特性及制种栽培技术要点,为节水抗旱水稻旱优3015的示范推广提供参考。

<正>香蜜杏品种从荷兰引进,也叫荷兰香蜜杏,俗称香杏。口感好,成熟晚,特别耐贮运,是名、特、优、稀、新的高效益杏品种;市场前景广阔,适合大面积发展。1品种特性1.1适应性强适应区域广泛,抗逆性强,耐寒、耐旱、耐瘠薄,适宜于南、北方大部分地区。管理相对较为简单容易,寿命较长。

Lilium spp. are perennial bulbous herbs that include ornamental， edible， and medicinal lilies all with high economic value. Since Lily is identified with complex genetic background， high heterozygosity， incompatibility in distant hybridization， long cycle of traditional cross breeding as well as low breeding accuracy， it is difficult to breed for new varieties. These modern breeding techniques， including crossbreeding， mutation breeding， polyploidization breeding， protoplast fusion breeding， and genetic engineering， have been applied in lily. This article reviews and summarizes the methods for overcoming the barriers of distant hybridization in lily hybrid breeding， the treatments in mutation breeding， the technical approaches in polyploidization breeding and haploid breeding， the methods in protoplast isolation and fusion in protoplast fusion breeding， and the advances in lily genetic transformation and editing. In addition， several examples in lily breeding methods and techniques are listed， and the problems in techniques are proposed. The future development and application prospects in lily breeding technologies are discussed. This article aims to provide insights for future research on lily breeding technologies and their applications in breeding of lily varieties.

In order to introduce and popularize fig cultivation in the mountainous area of Wulian County in Shandong Province, the climatic suitability analysis of fig cultivation in Wulian was carried out based on the local meteorological data over the years. In the study, we took the biological characteristics of fig into consideration, discussed the climate suitability of fig cultivation in Wulian, as well as common meteorological disasters and their prevention measures, and analyzed the influence of main local climate conditions on the growth of fig. The results show that the climate conditions of Wulian can basically meet the growth and development of fig if proper prevention measures are taken against meteorological disasters. This study provides reference for popularizing fig cultivation, preventing meteorological disasters and revitalizing rural economy in Wulian County.

The production and planting experience of a family farm in Xiaoxian, Suzhou, Anhui Province was combined, and the suitable environment, facility conditions, and key cultivation techniques for the growth of Morchella were summarized and analyzed. The cultivation of this fungus should be carried out on land with good air quality, flat terrain, and convenient drainage and irrigation. The soil should be loose, weakly alkaline, and well-permeable sandy loam; the cultivation facilities mainly consist of solar greenhouses, equipped with drip irrigation belts and atomization devices. The key points of facility cultivation techniques include selecting excellent varieties with mushroom shape, color, stable seed quality, thick meat texture, and high-yield; carry out soil plowing, disinfection, and prepare external nutrient packages before sowing; fine sowing, cover with black plastic film; do a good job in post-sowing management such as watering and mulching, temperature and light regulation, nutrient package placement, and mushroom urging; manage the mushroom production period in accordance with the requirements of each period; comprehensively applying agricultural methods, physical methods, biotechnology, and chemical techniques to effectively prevent and control bacterial contamination and pest infestations during the growth process of mushrooms; according to the principle of “picking large and keeping small”, harvest in a timely manner, and store or transport in a timely manner after harvesting. This article provides references for the development of facility cultivation industry of Morchella.

The planting performance of Zhuliangyou 5298 was combined in Anqing City, Anhui Province area, and its high yield cultivation techniques were summarized. From 2022 to 2024, this variety was planted in 12 demonstration sites, including Wangjiang County in Anqing City, with an upright and upward curved plant shape; the growth period was 130-136 days, with an average seed setting rate of 85.77%, a thousand grain weight of 24.1 g, and a yield of 8 280-9 000 kg/hm²; good polished rice yield and excellent rice quality; strong anti lodging ability. Its high yield cultivation techniques include early sowing (sowing and seedling cultivation from late April to mid May), timely transplanting, cultivating strong seedlings, keeping the soil of the seedling field moist, applying “weaning fertilizer” (urea 60-75 kg/hm²) according to the growth of the seedlings, and timely prevention and control of seedling diseases and pests such as rice thrips and bakanae disease; select high speed rice transplanter operation based on seedling quality, planting time, etc., inspect and debug the transplanting machinery according to the settings, determine the planting distance, seedling amount, and depth; timely weed control (spraying pesticides such as butachlor for soil sealing, spraying pesticides such as butazone for stem and leaf control during the seedling stage, and spraying pesticides such as 30% propiconazole for sealing throughout the field after the seedlings); heavy application of base fertilizer (45% compound fertilizer 450-600 kg/hm²), early application of tillering fertilizer (high nitrogen and high potassium compound fertilizer 225-300 kg/hm²), and supplementary application of ear fertilizer (potassium chloride 112.5-150.0 kg/hm²); shallow water seedling planting, deep water live planting, alternating dry and wet conditions to promote tillering; appropriate pesticides should be used during the growth period of the field to prevent and control diseases and pests such as rice blast and sheath blight. This article provides a reference for further promotion and planting of this variety in similar regions.

【Objective】By analyzing the genetic and breeding selection effects of the stable major QTL for spike length in wheat, its genetic effects on yield-related traits were clarified, and the future breeding application potential was evaluated. The results could provide a basis for subsequent gene mining and molecular breeding of wheat. 【Method】A major QTL for spike length, named qSl-2D, was detected in multiple environments using a recombinant inbred lines population derived from the cross of Kenong9204 and Jing411, denoted as KJ-RIL; Two molecular markers closely linked to qSl-2D were developed by using the InDel sites in target interval. The genetic effects of yield-related traits based on KJ-RIL, MY-F₂, NILs and natural mapping populations, were analyzed by combining genotype data of molecular markers or wheat 55K array, respectively. By genotyping the natural mapping population, the breeding selection effect of qSl-2D haplotype was parsed across different wheat regions and different ages. 【Result】QTL mapping results showed that qSl-2D could be detected in 7/10 sets of environmental data, and could explain 4.02%-10.10% of the phenotypic variation. The peak LOD of 5/10 sets of environmental data was positioned at 608.75 Mb. The results of genetic effect analysis showed that the enhancing allele of qSl-2D could significantly increase spike length in the four populations with different genetic backgrounds. In addition, it has positive effects on kernel number per spike and plant height, but has negative effects on thousand kernel weight, kernel weight per spike and yield per plant in most population backgrounds. Further analysis of plant height in KJ-RIL population showed that the enhancing allele had rod lowering effect on all internode lengths except the internode length below spike, which resulted in the insignificant increase in plant height. The results of qSl-2D haplotype analysis showed that the utilization rates of the long-spike haplotype Hap-AA-GG varied greatly in different wheat regions, with the highest utilization rate in the northern winter wheat region, accounting for 24%; while the short-spike haplotype Hap-CC-CC accounted for more than 30% in most wheat regions. Moreover, the utilization rate of qSl-2D long-spike haplotype showed a gradual decrease over time, while that of short-spike haplotype consistently maintained a higher selection trend. 【Conclusion】A stable major QTL-qSl-2D for spike length was identified, the enhancing allele of qSl-2D could significantly increase spike length under different genetic backgrounds, and had certain genetic effects on yield-related traits. The closely linked molecular markers developed in the target region can be used for the genetic improvement of wheat spike length and yield-related traits in wheat.

【Objective】Eestern and Southern Gansu is one of the epidemic centers of stripe rust and over-summering region for Puccinia striiformis f. sp. tritici (Pst) with continuous emergening of new Pst races in Northwestern China. The objective of this study is to analyze the genetic architecture of stripe rust resistance in winter wheat cultivars grown in those regions since 1990s for a better genetic diversity control of the disease, breeding of durable resistant cultivars, sustainable green and healthy ecological agriculture in Southern Gansu. 【Method】The seedling and adult plant stage resistance to the prevailing Pst races CYR33, CYR34, etc. of 117 wheat cultivars (lines) were evaluated at greenhouse in 2021 and field trials in Qingshui of Gansu and Pixian of Sichuan, respectively, during 2019-2020 and 2020-2021 cropping seasons. Molecualr markers for 15 Yr genes were also applied to detect the presence of known stripe rust resistant genes. 【Result】Of the 117 varieties and lines tested, thirty-four (29.1%) were found to perform adult plant stage resistance (APR) in the field, among these, 25.6% and 3.4% of cultivars were released in Southern Gansu, and Eastern Gansu, respectively. Another 25.6% susceptible cultivars from Southern Gansu exhibited slow rusting with disease severity (DS) less than 20%. Eighty-two cultivars (70.1%) were resistant to the race CYR33 of Pst at the seedling stage. Among them, 67 (57.3%) and 15 (12.8%) were planted in Southern and Eastern Gansu, respectively. However, only seven (6.0%) of the total entries were resistant to the dominant race CYR34 at the seedling stage, and all of these were cultivars from Southern Gansu, such as Lantian 131 etc. The stripe rusts resistant cultivars named as Lantian, Zhongliang, and Tianxuan series at both seedling and adult plant stages were mainly released after 2010. Molecular markers screening identified the presence of Yr9 (49.6%), Yr10 (1.7%), Yr17 (12.8%), Yr18 (7.7%), Yr26 (12.8%), Yr28 (20.5%), Yr29 (10.3%), Yr30 (34.2%), Yr41 (2.6%), Yr46 (16.2%), YrZH22 (15.4%) and YrZH84 (27.4%) in some cultivars, preferly as 2-5 genes combinations in 73 (62.4%) cultivars. Pyramiding of YrZH84, YrZH22, and Yr17 with other stripe rust resistance genes could provide better disease resistance than other gene combinations. In addition, high frequency of Yr10, Yr17, Yr18, Yr28, Yr29, Yr30, Yr41, and Yr46 was detected in wheat cultivars grown in the dry highland Pst oversummering region. However, Yr26, Yr30, YrZH22, and YrZH84 were mainly identified in wheat cultivars of the valley Pst overwintering region, indicating significant genetic architecture difference for the stripe rust resistance genes between the cultivars of the oversummering and overwintering regions. Higher genetic diversity of stripe rust resistance genes was found in the cultivars of the oversummering region, compared with that released in the overwintering region. 【Conclusion】Our results revealed the current status of stripe rust resistance genes and their utilization in winter wheat cultivars of Gansu, the northwest oversummering region for Pst of China, in the past 20 years. Diversification of stripe rust resistance genes have been successfully applied in the winter wheat breeding program to develop commercial wheat cultivars and lines for sustainable control of the stripe rust disease. The development of wheat cultivars with stacked stripe rust resistance genes has solved the historical problem of wheat cultivars with narrow genetic background and mono-resistance gene, domonstrating the successful control of wheat stripe rust epidemics using genetic diversity of wheat resistance gene in this region. The present study provides theoretical basis for genetic diversity control of stripe rust disease and set an example for the sustainable green ecological agriculture by breeding wheat cultivars with durable disease resistance.

An overview of traditional and modern breeding techniques currently used for vegetable crops was provided. Traditional breeding includes extensive hybridization, inbreeding, etc. Although it can improve crop traits, it has limitations such as low efficiency and high cost. The introduction of modern breeding techniques such as mutagenesis and genome editing can effectively improve breeding efficiency and accuracy; the progress of genome sequencing and functional genomics has provided the possibility for a profound understanding of vegetable genomes and promoted precise improvement of traits; molecular marker technology and transgenic technology have also provided new means to improve vegetable yield, quality, and stress tolerance. By using these technologies for vegetable crop breeding, the adaptability and economic value of crops can be enhanced, ensuring food safety and nutritional requirements. In the future, we will continue to explore the potential of genome assisted breeding and apply technologies such as big data and artificial intelligence to vegetable breeding, providing references for the development of high-yield, disease resistant, and stress tolerant vegetable varieties.

【Objective】To develop high-yielding and FHB-resistant wheat cultivars in the Yellow and Huai River Valley Winter Wheat Zone (YHWZ), simultaneously improving of yield and resistance was conducted in this study.【Method】Using the elite parent dwarf male sterile (DMS) wheat combined with double haploid (DH) technology and molecular marker assisted selection (MAS) of Fhb1 (DMS wheat molecular breeding strategy), DH lines were developed using Sumai 3 as a donor (FHB- resistant parent) and Zhoumai 16’s DMS wheat, Zhoumai 16, Lunxuan 136 and Lunxuan 6 as recipient parents. The agronomic traits (plant height, heading date, yield, etc.) and FHB resistance were evaluated for these DH lines.【Result】A total of 51 Fhb1-DH lines characterized by facultative growth habit, semi-dwarf and white grains were selected using this strategy. The average number of infected spikelets of 51 lines were 5.7 and 7.3 at the 2020Henan and 2020Beijing sites, respectively, and average disease severities were 27.7% and 35.2%, which is not different from moderately susceptible control Huaimai 20. There was no significant difference in grain yield per hm² between the mean performance of the 51 lines and the control Zhoumai 18. DH116 (Lunxuan 20), a promising line from the 51 lines, was further evaluated for FHB resistance and agronomic traits in multiple environments. The resistance of Lunxuan 20 to FHB was significantly improved, and no significant difference was found in the number of infected spikelets or disease severity between Lunxuan 20 and moderately or highly resistant controls at four sites. Lunxuan 20 showed slightly greater grain yield per hm², and significantly higher number of spikelets per spike and thousand grain weight (P<0.05), earlier heading date and shorter plant height (P<0.05) than the control Zhoumai 18 in two environments. The grain yield per hm² of Lunxuan 20 was 4.6% and 1.7% higher than the control cultivar Bainong 207 in the two list trials of Henan Province, and 3.5% higher than Bainong 207 in the demonstration trial. Resistance of Lunxuan 20 to FHB ranged from moderate susceptibility to moderate resistance in two-year list tests using the single-floret injection and spray inoculation methods. Lunxuan 20 carries the semi-dwarfing gene Rht-D1b at the Rht-D1 locus, and the recessive alleles vrn-A1, vrn-B1 and vrn-D1 associated with the winter growth habit at the Vrn-A1, Vrn-B1 and Vrn-D1 loci. Based on the wheat 660K single nucleotide polymorphisms (SNPs), 64.7% of the SNPs were shared by Lunxuan 20 and its parents, and the direct genetic contributions of Zhoumai 16, Lunxuan 136, Lunxuan 6 and Sumai 3 to Lunxuan 20 were 69.8%, 12.6%, 6.1% and 11.5%, respectively.【Conclusion】A high-yielding and FHB-resistant wheat cultivar Lunxuan 20 was bred using the DMS wheat molecular breeding strategy.

The large-scale planting performance of drought resistant rice variety Hanyou 73 was combined in Donghu Production Base, Dongqiao Town, Huian County, Xiamen, Fujian Province, its “rice+vegetable” water-dry rotation cultivation techniques and its advantages in arid land were summarized and analyzed. This variety was planted in a “rice+vegetable” rotation in the research area, with a total growth period of about 128 days and resistant to bacterial leaf blight and rice blast disease, with good drought resistance; the dry grain yield of rice was 7 542.75 kg/hm². Dryland cultivation techniques include land preparation, application of base fertilizer, and rotary tillage; after seed mixing or seedling cultivation, live broadcasting or waterless machine transplanting is carried out; arrange micro sprinkler irrigation pipe belts in the field according to the walking route of the rice transplanter wheels; after emergence, water the seedlings with water for greening, tillering, and heading filling to improve seedling quality and rice seed setting rate; according to the seedling situation in the field, apply tillering fertilizer, jointing fertilizer, and heading fertilizer to cultivate strong seedlings; dryland has more grass damage than paddy fields, and generally adopts measures such as closed weeding as the main method, chemical pesticides as auxiliary, and manual removal and remediation for prevention and control; according to local pest and disease monitoring information, timely prevention and control of diseases and pests such as sheath blight and rice planthopper. The application of the “rice+vegetable” water-dry rotation model and dryland waterless machine transplanting technology has technical management advantages such as saving labor and reducing consumption, facilitating water control, and improving fertilizer utilization efficiency; and social benefits such as improving farmland utilization efficiency, conserving water resources, and improving soil environment. This article provides a reference for the promotion and application of the “rice+vegetable” water-dry rotation model in relevant region’ dry land.

The cutting propagation technology experiment of Rosa chinensis var. minima ‘Petit Four’ with constant temperature substrate breeding system has been done. The effects of substrate temperature, micro spraying times, micro spraying duration and hormone treatment on the cutting rooting were studied with orthogonal design L₉(3⁴), so as to improve the breeding technology system of Rosa chinensis var. minima ‘Petit Four’. The results showed that by comprehensively analyzing the average rooting rate, average root length and average rooting number, the optimal horizontal combination was A₃B₂C₁D₁, that is, the substrate temperature was 25℃, the micro spraying was 2 times, the micro spraying duration was 5 minutes, soaked with IBA 200 mg/L for 10 minutes. The best treatment in this experiment was T8 treatment, with an average rooting rate of 91.08%, an average root length of 5.17 cm, and an average number of 6.36 roots.

我国是世界上最大的生猪生产国和猪肉消费国，但仍存在着母系猪繁殖力普遍较低的重要问题，选育具有高繁殖性状的母系猪已成为当前研究的焦点和热点。目前，已明确多个影响母猪产仔数的已知基因，包括雌激素受体(estrogen receptors,ESR)基因、泌乳素受体(prolactin receptor,PRLR)基因、视黄醇结合蛋白4(retinol binding protein 4,RBP4)基因、瘦素(leptin,LEP)基因、备解素(complement factor b,BF)基因、胰岛素样生长因子结合蛋白(insulin-like growth factor binding protein,IGFBP)基因、连环蛋白阿尔法样蛋白1(catenin alpha-like protein 1,CTNNAL1)、无翼型MMTV结合位点家族10B(wingless-type mmtv integration site family member 10B,WNT10B)基因、转录因子12(transcription factor 12,TCF12)基因、无精症样删除基因家族(deleted in azoospermia-like,DAZL)、无名指蛋白4(ring finger protein 4,RNF4)基因以及骨形成蛋白(bone morphogenetic proteins,BMPs)家族等基因。这些基因通过复杂的相互作用网络影响母猪的繁殖力性状表现，但少数几个基因的位点效应对母猪的繁殖力表型影响较为有限，因此在母猪繁殖性能方面的育种遗传进展相对较小。全基因组关联分析(genome-wide association studies, GWAS)技术基于全基因组策略，利用覆盖全基因组的遗传标记信息，分析整个基因组中的全部遗传变异多态性作为分子遗传标记，并与表型和系谱信息进行对照和统计分析，从而加速了重要单核苷酸多态(single nucleotide polymorphisms, SNPs)位点、数量性状位点(quantitative trait locus, QTLs)和候选基因的发现过程。全基因组选育(genomic selection, GS)利用系谱信息、表型数据以及全基因组的SNP分型信息，为母猪繁殖性能等低遗传力性状的育种工作提供了更快速、准确的个体全基因组估计育种值(genomic estimated breeding value, GEBV),从而显著加快了育种遗传进展。

With the advancement of agricultural supply-side structural reforms and the growing demand for high-quality, safe, and eco-friendly agricultural products in China, cotton production now faces the challenge of coordinating multiple objectives, including yield enhancement, quality optimization, simplified and efficient management, and environmental sustainability. To address these challenges, this paper proposes the novel concept of multi-objective collaborative cultivation (hereafter termed “collaborative cultivation”). We systematically elaborate on the theoretical foundations underpinning this approach, including mechanisms of precision sowing for robust seedling establishment, synergistic water-fertilizer management under partial root-zone irrigation, population regulation through high-density planting with chemical regulation and pruning-free canopy shaping, physiological mechanisms of defoliation-ripening for synchronized boll maturation, and compensatory growth strategies ensuring yield stability under abiotic stress. Building on these theorical bases and international research insights, we identify four core technologies of collaborative cultivation: (i) precision sowing coupled with stress-resilient seedling establishment under adversity, (ii) high-density planting with chemical regulation for canopy shaping, (iii) variable-rate drip irrigation with water-fertilizer synergy management, and (iv) synchronized maturation control technology. Empirical evaluations demonstrate that the integrated application of these technologies optimizes resource utilization, enhances productivity, and ensures fiber quality consistency, while reducing labor inputs and chemical usage. Case studies from major cotton-producing regions validate that collaborative cultivation achieves synergistic outcomes in productivity, sustainability, and economic viability, aligning with green agricultural development goals. Future research priorities include optimizing multi-objective trade-offs, deciphering genotype-environment-management interactions, enhancing stress compensation mechanisms, and extending collaborative principles to multi-cropping systems. Through interdisciplinary innovation and technology integration, this framework offers a systemic solution for high-quality cotton industry development, demonstrating significant potential to drive the sector's green transformation and sustainable advancement.

Handou 13 has been widely used in soybean and corn belt compound planting in Huang-Huai-Hai region, and has obtained high-yield and benefit. The high-yield cultivation techniques of the ribbon compound of this variety were summarized and analyzed. This soybean variety had the characteristics of shade tolerance, high oil content, density tolerance, lodging resistance, high-yield, and suitable mechanical harvesting when planted in this area. Its high-yield cultivation techniques included selecting coated soybean seeds to prevent underground pest infestation and increase germination rate; planting soybean and corn in a 4∶2 ratio to ensure appropriate density. In terms of field management, proper watering should be done during the grain filling period, nutrients should be added according to crop needs, insect pests such as soybean spot bug and whitefly should be prevented scientifically, and take measures such as soil sealing and stem and leaf control to prevent weeds in the field, etc., so as to improve yield potential. Timely and appropriate spraying of plant growth regulators to control vigorous plant growth; choose joint harvesting machinery for timely harvesting, etc. This article provides references for the application of this variety in soybean corn ribbon compound planting.

In this paper, the demand characteristics of konjac growth environment were summarized. The research progress in recent years on cultivation modes of konjac in China such as yard cultivation, monoculture, intercropping cultivation in field with other crops, understory cultivation, rotation cultivation and protected cultivation were reviewed, and the advantages and disadvantages of the above cultivation modes were analyzed. It is proposed that, in the future, the konjac germplasm resources should be collected, identified, evaluated and utilized extensively, new cultivation modes of intercropping and rotation should be constructed, the mechanization and automation level of konjac cultivation should be improved, the cultivation area of konjac should be expanded, and organic cultivation mode should be established, so as to enrich the cultivation modes of konjac.

<正>水红秦冠是20世纪90年代在静宁县贾河乡中堡村发现的秦冠变异品种，外观艳丽，受到客商重视，客商高价收购后，到市场试销，获利颇丰，其后一直按片红富士收购，多年来保持畅销俏销。良好的市场销售前景，刺激其种植面积不断扩大，目前仅静宁种植面积超过5万亩，静宁周边的庄浪、天水、通渭也广泛种植，近年来静宁县果树研究所申请进行了省级品种鉴定，命名为“静宁一号”，开始作为静宁苹果主栽品种之一，进行推广普及（图1）。

Based on the current breeding status of potatoes, the aspects that need further improvement, and corresponding development strategies and suggestions were summarized and analyzed. At present, potato breeding was mainly based on its asexual reproduction characteristics, which had the advantages of simplicity, effectiveness, and ensuring the purity of potato seeds. Meanwhile, it was necessary to do a good job in potato seed potato detoxification, pest control, and field management. In the breeding process, it was necessary to further improve the collection of potato variety resources, the production capacity of seed potatoes, the production technology of seed potatoes, the quality of virus-free seed potatoes, and the processing capacity of potato products. Development strategies for potato breeding work were proposed, including establishing a good breeding environment, scientifically introducing potato varieties, innovating potato breeding methods, and innovating production equipment and technology, to provide references for promoting the standardized and rational development of the potato industry.

In view of the shortage of water and heat resources in different ecological areas, and the frequent occurrence of wind disaster, lodging, low yield, poor quality and other problems in maize production, a new maize variety ‘MC278’ was bred by taking ‘Jing X005’ as female parent and ‘Jing 27’ as male parent. The inoculation tests of the variety in Jilin, Hebei and other places showed that it had strong resistance to small spot disease, Curvularia, dwarf mosaic, silk smut and stem rot, and was easy to infect large leaf spot, and it was moderately resistant to corn borer in Jilin. In the regional trials in Beijing, Inner Mongolia and other regions in different years, the kernel yield of ‘MC278’ was significantly higher than that of ‘Zhengdan 958’ (CK), the highest yield was 16166 kg/hm², and the average yield was increased by 8.3%. The content of crude protein, crude starch and lysine of ‘MC278’ were significantly higher than those of ‘Zhengdan 958’. In 2019, the production in Hebei, Shanxi, Inner Mongolia and other regions showed that the yield of ‘MC278’ was all more than 12804 kg/hm², and the maximum yield was 14283 kg/hm². ‘MC278’ was equipped with field management measures and harvest techniques, such as suitable sowing date, density and water and fertilizer management, and prevention and control measures of diseases and insect pests. The variety passed the national examination and approval in 2019 (National Approved Maize Variety No.20190030), and its planting area now includes 15 provincial regions, such as Anhui, Shanxi, Shandong and etc.

Sugar beet is one of the major sugar crops in the world, and it has high sugar yield and good economic value. It is an indispensable crop to solve the problem of sugar security in China and one of the crops with very successful heterosis utilization. In order to rapidly select sugar beet varieties with high yield, high sugar and strong stress resistance, sugar beet breeding is gradually developing from conventional breeding to biological breeding, biological breeding will be the future direction of sugar beet breeding. In this paper, the biological breeding methods of sugar beet that have been applied in recent years were summarized, including molecular marker technology, haploid induction technology, genetic engineering, cell engineering and omics technology, the future development of beet biological breeding was also prospected.

To establish an efficient and sustainable RNAi breeding control system, this paper summarizes the application potential of RNAi technology in crop disease and pest resistance, reviews the current research and development status of transgenic RNAi crops, and analyzes the design strategies and synergistic resistance mechanisms of multi-target tandem RNAi. The key points of elaboration include the "dsRNA/microRNA stable expression system", the "combined control model of complex pests and diseases (wheat scab - aphids, cotton wilt - cotton aphids)", and the "precise gene intervention approach for delaying resistance evolution", etc. It is pointed out that low delivery efficiency, poor environmental stability of dsRNA and high production cost remain the bottlenecks for large-scale application. This paper proposes that through the precise release technology of nano-carrier-plant symbiotic delivery, tandem expression of multi-gene silencers, and combined with ecological balance monitoring, the coordinated management of multiple pests and diseases can be achieved within 5 to 10 years. It is believed that this system will promote the transformation of agriculture towards a sustainable model of "precise genetic intervention + ecological balance maintenance", providing key support for global food security.

In this paper, the research progress of pineapple cultivars-breeding and cultivation practices was summarized, including current advances in research on pineapple gernplasm resources, hybrid breeding, mutation-selection breeding, genetic engineering breeding, as well as high and efficient cultivation practices such as seedling propagation, plant technology, flower forcing, cold- and sunburn- proofing. Also, the future research directions of pineapple breeding and cultivation practices were discussed. The major future research focus of pineapple breeding is to establish a new pineapple breeding system with hybrid breeding and mutation-selection breeding as the main body, and genetic engineering breeding as the assistant procedure based on the accurate evaluation of pineapple germplasm resources. It is also important to carry out the research on the mechanism of the occurrence of the black heart disease of pineapple and the transparent pineapple as well as the cultivation practices to reduce the occurrence of the two diseases. In addition, the study on non-destructive testing technology and the intelligent and labor-saving planting technology are the necessary parts of pineapple research.

Camellia oleifera as a unique woody edible oil tree species, has both economic value and ecological maintenance functions. The techniques for high-yield cultivation of Camellia oleifera forests combined its biological characteristics were systematically explored, including selection of afforestation sites, superior varieties, and others. Camellia oleifera trees are 2-8 meters tall, with spherical or nearly spherical fruits that generally mature in autumn. The key points of high-yield cultivation techniques include selecting areas with an altitude of 100-500 meters, sufficient sunlight, and slopes below 25 degrees for planting; scientifically apply nitrogen, phosphorus, and potassium fertilizers based on the nutrient status of the soil and the growth needs of plants to improve the soil; select varieties with larger fruits, thinner skin, and higher seed and oil extraction rates; choose strong seedlings that are one or two years old, ensuring their good growth and strong stress resistance; excavate planting holes and adopt reasonable methods for seedling planting; reasonably irrigate and reasonably fertilize oil tea trees based on their age, soil texture, and local climate conditions; after the oil tea is harvested and before the new shoots sprout in spring, remove diseased and weak branches in a timely manner; adopt agricultural, biological, and chemical control measures to timely prevent and control diseases such as anthracnose and soft rot, as well as pests such as Biston marginata and Euproctis pseudoconspersa; when the fruit is 80% to 90% ripe, it should be harvested and processed promptly to prevent spoilage. These technological lare interrelated and together form a complete, efficient, and sustainable high-yield cultivation techniques system for Camellia oleifera, provide references for the efficient development of Camellia oleifera and its related industries.

<正>一、狐狸繁殖期饲养方法与管理1.配种前准备。（1）狐狸繁殖特性。狐狸是季节性发情的自排卵动物，属哺乳纲食肉目犬科动物。春节繁殖，一年繁殖1次，5～10只/胎。山东省潍坊市狐狸养殖多以北极狐为主，从8月开始，雌狐卵巢、雄狐睾丸均开始发育，雌狐黄体逐渐退化，卵泡迅速增长。11月起雄狐发育明显，此时可产生成熟精子。翌年春季，雌狐开始发情、排卵。

从大豆属物种的系统进化和栽培大豆起源研究的方法等方面评述了栽培大豆中国东北起源、黄河中下游起源、长江流域及南方起源、日本南部起源等多种假设的依据。在此基础上讨论了多样性中心与起源中心的关系、栽培物种起源与演化的研究方法,以及运用比较实验生物学研究作物进化时的技术性问题。作者倾向于支持栽培大豆南方起源假设。