The present study aims to understand the current situation of research activities in biotechnology field in China, and thus provide reference for the researchers, research management department and journal editors. Using Bibliometric analysis method, we analyzed the distribution of domestic and abroad biotechnological papers published from 2005 to 2009 by the authors from Chinese Academy of Fishery Sciences, in the aspects of paper content, author and organization. The results showed that the overall amount of the articles published by the authors from Chinese Academy of Fishery Sciences experienced great growth. The authors involved in genetic breeding published the most articles. The most key words were micro-satellite, gene clone, gene expression and sequence analysis which present the current research focuses. Trait-related gene, pedigree identification, karyotype, genome and gynogenesis were the emerging key words which indicated new research field. Core authors were selected out and co-citation between authors were analyzed using the method of social network analysis. From the above, it could be concluded that in recent years, the number of biotechnology articles published by the authors from Chinese Academy of Fishery Sciences increased steadily, and domestic paper number was much higher than international ones. Biotechniques such as micro-satellite were widely used and promoted great improvement of genetic diversity and genetic breeding. Close relationship between researchers had not came into being yet and the research production and research influence did not distributed equilibriumly in every institute of Chinese Academy of Fishery Sciences.

The aim was to provide a reference and theoretical basis for genetic breeding work of Asparagus in the future. This paper reviewed the advances in the past few decades on the application of biotechnology to Asparagus breeding, including tissue culture, anther culture, microspore culture, protoplast culture, polyploidy induction, molecular marker technique and genetic transformation. The review highlighted the role and future directions of applying biotechnology to Asparagus breeding, and analyzed existing problems in such applications. In the end, according to the biological characteristics of Asparagus and the demand of Asparagus industry, the research trend on biotechnology breeding of Asparagus was also discussed.

In order to provide new ideas for flax germplasm innovation and promote further development of flax breeding, current research status of flax breeding was described briefly, and the application of gene engineering, plant tissue culture and molecular marker in flax breeding was summarized in the paper. We proposed that the emphasis of flax breeding was to strengthen the study on distant hybridization and improve the efficiency and effect of hybridization breeding by using marker- assisted selection. Establishing and perfecting an effective technical system of anther culture were the keys to establish a practical breeding technical system and breed elite new cultivars. Furthermore, establishing an efficient genetic transformation breeding technical system was an important direction of flax breeding.

In agricultural production, the use of chemical fertilizers and chemical pesticides can increase the total yield and the yield per unit area, but affect the safety of agricultural products and the protection of ecological environment. In order to solve this problem, the agricultural operation mechanism should be innovated, to develop biological agricultural technology, accelerate the transformation and upgrading of agriculture, and promote the agricultural development with the characteristics of “high yield, high quality, high efficiency, ecology and safety”. Taizhou San'an Heheng Organic Agricultural Products Professional Cooperative has carried out a great deal of research on this subject in the past 10 years, using biological technology to cultivate 2670 hm ² of rice. The national authorities have conducted tests on more than 200 items of chemical residues and pollutants in the mature crops produced by the cooperative, and the rice quality has reached the GB/T 19630 standard of Zero Agricultural Chemical Residue and Low Pollutant Limit. The practice of San'an Heheng Organic Agricultural Products Professional Cooperative has proved that the integrated technology of biological agriculture can not only obtain the rice output equivalent to chemical production mode, but also produce safe agricultural products. At the same time, it can protect the ecological environment, increase farmers’ income, improve agricultural efficiency, and finally realize the goal of "high quality, high yield, high efficiency, ecology and safety" in agriculture.

This paper summarizes the applications of modern biological technology, such as vegetative compatibility groups (VCG), restriction fragment length polymorphisms (RFLP) and inter-simple sequence repeat (ISSR), rDNA-ITS, translation elongation factor1-α (EF-α) sequence analysis in the research of classification system and an identification method of Fusarium, and obtains molecular biology such as RFLP and ISSR were used to analysis the correlation between individuals and geographical origin strains, rDNA-ITS, EF-α sequence analysis had provided the basis for classification and identification about Fusarium at the level of species and subspecies, the VCG was used for distinguish different genetic structure of the group that lack teleomorph. The future direction was discussed.

Flax fiber is widely used in agriculture, medical treatment, industry and aerospace industry. With the rapid development of molecular biology technology and genetic engineering technology, the research of breeding method has become the key to culture new flax plant. In order to accelerate the breeding efficiency and improve the quality and yield of flax fiber, this article summarized the application of genetic engineering technology, tissue culture technique and molecular markertechnology in fiber flax breeding, and elaborated the latest research progress of genetic engineering application in flax quality and character, protoplast fusion and culture technique in flax breeding. The study could provide certain references for the research and development of flax resource.