Cover cropping is a diversifying agricultural practice that can improve soil structure and function by altering the underground litter diversity and soil microbial communities. Here, we tested how a wheat cover crop alters the decomposition of cucumber root litter. A three-year greenhouse litterbag decomposition experiment showed that a wheat cover crop accelerates the decomposition of cucumber root litter. A microcosm litterbag experiment further showed that wheat litter and the soil microbial community could improve cucumber root litter decomposition. Moreover, the wheat cover crop altered the abundances and diversities of soil bacterial and fungal communities, and enriched several putative keystone OTUs, such as Bacillus spp. OTU1837 and Mortierella spp. OTU1236, that were positively related to the mass loss of cucumber root litter. The representative bacterial and fungal strains B186 and M3 were isolated and cultured. In vitro decomposition tests demonstrated that both B186 and M3 had cucumber root litter decomposition activity and a stronger effect was found when they were co-incubated. Overall, a wheat cover crop accelerated cucumber root litter decomposition by altering the soil microbial communities, particularly by stimulating certain putative keystone taxa, which provides a theoretical basis for using cover crops to promote sustainable agricultural development. 

Large grain is a favorable trait for appearance quality and large sink potential in wheat breeding.  A stable QTL QGl.caas-5BS for grain length was previously identified in a recombinant inbred line population from the cross of Zhongmai 871 (ZM871) and its sister line Zhongmai 895 (ZM895).  Here, a BC₁F₆ residual heterozygous line was selected from the cross of ZM871/ZM895//ZM871 population, and six heterozygous recombinant plants were identified in the BC₁F₇ population from self-pollination of the heterozygous line.  QGl.caas-5BS was delimited into an interval of approximately 2.2 Mb flanked by markers Kasp_5B33 and Kasp_5B2 (25.3-27.5 Mb) through phenotyping and genotyping the secondary mapping populations derived from these heterozygous recombinant plants.  Five genes were predicted as candidates of QGl.caas-5BS based on sequence polymorphism and differential expression analyses.  Further mutation analysis showed that TraesCS5B02G026800 is likely the causal gene of QGl.caas-5BS.  A gene-specific marker Kasp_5B_Gl for TraesCS5B02G026800 was developed, and a significant genetic effect of QGl.caas-5BS on grain length was identified in a validation population including 166 cultivars using the marker.  These findings lay a good foundation for map-based cloning of QGl.caas-5BS and provide a breeding-applicable marker for the improvement of grain length in wheat.

Late sowing is a critical factor that hinders achieving high-yield, good-quality wheat under rice-wheat rotation.  Understanding the physiological basis and regulatory pathways that lead to late-sown wheat of high yield and good quality is crucial for developing effective cultivation strategies.  A 2-year field experiment was conducted to investigate the effects of sowing date, nitrogen (N) application rate, and planting density on wheat yield, grain quality, population characteristics, and the underlying physiological factors.  The results revealed significant interactions among the sowing date, planting density, and N application in regulating both yield and quality. Late sowing reduced grain yield primarily by reducing the number of spikes and kernels.  However, the latter was improved by increasing N application and the planting density, thus mitigating the yield losses caused by late sowing.  Moreover, the grain protein content (GPC) and wet gluten content (WGC) increased with delayed sowing dates and higher N rates, but decreased with increased planting densities.  For wheat yields over 9,000 or 7,500 kg ha^–1, the latest sowing date should not be later than Nov. 4 or 15, respectively.  In addition, specific criteria should be met, including a maximum of 1.5 and 1.0 million stems and tillers ha^–1, a maximum leaf area index of 6.7 and 5.5, and a dry matter accumulation (DMA) at anthesis of 14,000 and 12,000 kg ha^–1, respectively. For high-yield, good-quality late-sown wheat, the optimal combination is a 25% increase in the N rate (300 kg N ha^–1) and a planting density of 2.25 million (N300D225) or 3.75 million (N300D375) plants ha^–1 for 10- or 20-day delays in sowing, respectively.  These combinations result in higher leaf net photosynthetic rate; higher activities of leaf nitrate reductase, glutamine synthetase, and grain glutamic pyruvic transaminase; and a lower sugar-N ratio during post-anthesis.

The study aims to screen superior wheat varieties suitable for cultivation under the current context of rapid wheat cultivar renewal and iteration, to provide references for achieving coordinated improvement of yield and quality in rice stubble wheat selection in the Lixiahe region. Using 13 different gluten-type wheat varieties including 'Yanmai 1' as test materials, a single-factor randomized block design was adopted. The grain yield was measured manually and converted to 13% moisture content. The water content, protein content and starch content were determined by near infrared analyzer. Test weight meter and hardness meter were employed to measure grain test weight and hardness. Flour yield was determined after milling and sieving. The wet gluten content and gluten index was determined with a gluten washing instrument according to the method of GB/T 5506.2-2008. The settlement value was determined by AACC 56-61A method. The data were processed and analyzed by SPSS with LSD method. Y1 and Z13 showed the highest yield, which were above 7700 kg/hm² and increased by 12.12% to 25.68% compared with other varieties, while simultaneously meeting national quality standards for medium-gluten wheat: grain hardness ≥60%, test weight ≥770 g/L, protein content ≥12.5%, and wet gluten content ≥26%. N13 demonstrated relatively high yield (6936 kg/hm²) with protein content (12.41%), wet gluten content (24.11%) and sedimentation value (24.32 mL) all reaching national standards for weak-gluten wheat. Other varieties showed moderate yield levels with more complex and overlapping quality indicators. This experiment demonstrates that 'Yanmai 1', 'Zhenmai 13' and 'Ningmai 13' respectively represent high-yielding and high-quality medium-gluten varieties, and stable-yielding high-quality weak-gluten varieties suitable for promotion in this region, laying a solid foundation for coordinated improvement of yield and quality in rice stubble wheat cultivation in the Lixiahe region.

To screen the wheat varieties suitable for planting in the Cd-contaminated farmland of Hebei Piedmont Plain, the field experiment was conducted to study the yield and differential characteristics of cadmium absorption of 48 local main wheat varieties. The results showed that there were significant differences in the yield and the ability to absorb and accumulate Cd among 48 wheat varieties (P<0.05). The yield of ‘Shimai 27’ was the highest (10538.6 kg/hm²), which was 6.33%-42.41% higher than that of other wheat varieties. The yield of ‘Shimai 26’ was the second highest, which was 6.77%-38.51% higher than that of other wheat varieties. The grain Cd contents and enrichment coefficients of ‘Shimai 26’, ‘Zhongxinmai 48’, ‘Malan 6’ and ‘Kenong 2011’ were relatively low, ranging from 0.115 to 0.133 mg/kg and from 0.032 to 0.038, respectively. The Cd transport coefficients of ‘Kenong 2011’, ‘Zhongxinmai 48’ and ‘Shimai 26’ were relatively low, ranging from 0.273 to 0.291. The results of cluster analysis showed that there were 2 varieties of low Cd accumulation, 19 varieties with relatively low Cd accumulation, 3 varieties with low Cd translocation, and 7 varieties with relatively low Cd translocation. By comprehensive evaluation of wheat yield, grain Cd content, enrichment coefficient and transport coefficient, ‘Shimai 26’, ‘Zhongxinmai 48’ and ‘Kenong 2011’ were identified as Cd low accumulation type wheat varieties for safe production in the Cd-contaminated farmland.

To investigate the effects of zinc (Zn) on seed germination and growth of wheat, two cultivars, Shangmai 5226 and Xiaoyan 15, were selected as experimental materials. 6 Zn concentration gradients (ZnSO₄ 0, 50, 100, 200, 400, and 800 mg/L) were set, with 0 mg/L as the control (CK). The germination indicators, growth indicators and physiological indicators of each group were measured. The results showed that in terms of germination parameters, as Zn concentration increased, the germination potential and germination rate of both wheat cultivars first increased and then decreased; at 100 mg/L Zn, Shangmai 5226 and Xiaoyan 15 exhibited higher germination rates and germination potentials. Regarding growth indicators, with increasing Zn concentration, shoot length, shoot fresh weight, and shoot dry weight of both cultivars initially increased and then decreased, whereas root length, root fresh weight, and root dry weight gradually declined. For physiological indices, as Zn concentration increased, chlorophyll content, soluble protein content, soluble sugar content, superoxide dismutase (SOD) activity, and catalase (CAT) activity in both wheat cultivars showed a trend of increasing first and then decreasing. In contrast, peroxidase (POD) activity and malondialdehyde (MDA) content progressively increased, while free proline content first decreased and then increased. In conclusion, Zn concentrations of 50-100 mg/L were conducive to the seed germination and seeding growth of Shangmai 5226 and Xiaoyan 15; Zn concentration exceeding 100 mg/L had a certain inhibitory effect on wheat seed germination.

To promote the technology of replacing chemical fertilizers with organic fertilizers and study its practical effects in wheat production, an experiment was conducted at Yanghu Farm in Sixian, Anhui Province. The experiment included 7 fertilization treatments: no fertilization, conventional fertilization, optimized fertilization, organic and inorganic combined fertilization 1 (organic fertilizer replacing 15% chemical fertilizer), organic and inorganic combined fertilization 2 (organic fertilizer replacing 30% chemical fertilizer), nitrogen substitution 1 (organic fertilizer replacing 15% nitrogen fertilizer), and nitrogen substitution 2 (organic fertilizer replacing 30% nitrogen fertilizer). Wheat yield, quality, economic benefits, and soil physicochemical properties were measured under different treatments. The results showed that both organic and inorganic combination and nitrogen substitution treatments increased wheat yield; beneficial for increasing the total protein and starch content of wheat grains and improving economic benefits; the use of organic fertilizers instead of chemical fertilizers had a promoting effect on the increase of available phosphorus, available potassium, total nitrogen, total phosphorus, total potassium, and organic matter content in soil. Overall, the application effect of replacing 15% nitrogen fertilizer with organic fertilizer (nitrogen substitution 1) is better, resulting in higher wheat yield and economic benefits.

To compare and analyze the effects of phosphorus and potassium migration treatment on the number and weight of grains in different parts of wheat spikelet under late-spring coldness, the varieties Yannong 19 with strong resistance to late-spring coldness and Xinmai 26 with weak resistance to late-spring coldness were used as experimental materials, the 6 treatments were seted up, including room temperature+all basic application of phosphorus and potassium fertilizers (CKR₁), room temperature+split application of phosphorus and potassium fertilizers (CKR₂), 4 ℃+all basic application of phosphorus and potassium fertilizers (T₁R₁), 4 ℃+split application of phosphorus and potassium fertilizers (T₁R₂), -4 ℃+all basic application of phosphorus and potassium fertilizers (T₂R₁), -4 ℃+split application of phosphorus and potassium fertilizers (T₂R₂), and the number of grains and thousand grain weight at different spikelet positions of wheat were measured at maturity. The results showed that the late-spring coldness treatment reduced the number of grains and thousand grain weight at the upper and lower spikelet positions of the wheat varieties tested, with little effect on the grain yield in the middle spikelet positions. The backward shift of phosphorus and potassium significantly increased the number of grains per spike and thousand grain weight of late-spring coldness wheat during the jointing and booting stage, thereby reducing the impact of late-spring coldness on wheat single spike weight. This article provides guidance on preventing and mitigating late-spring coldness in wheat production.

To determine the aerial application effect of 19% picoxystrobin·propiconazole microemulsion on wheat scab, 5 treatments were set up, namely 19% picoxystrobin·propiconazole microemulsion, 48% cyanothiazole·tebuconazole suspension, 30% prothiazole dispersible oil suspension, 30% triazole ether · fluconazole suspension and clear water control treatment (CK). Field aerial application experiments were conducted using unmanned aerial vehicles. Observe the safety of the agent and the phenotypic traits of wheat, calculate the control effect and determine its yield. The results showed that 19% picoxystrobin·propiconazole microemulsion had good safety for wheat growth. After treatment, the wheat leaves were wax-yellow to light yellow, the ears and stalks were wax-yellow, and the maturity appearance was relatively good. 7 days after the second application of the drug, the prevention and control effect was better. The rate of diseased clusters, the control efficacy of diseased clusters, the disease index and the control efficacy of diseased fingers were 0.20%, 95.45%, 0.03 and 95.65% respectively. 28 days after the second administration of the drug, the rate of diseased clusters, the control effect of diseased clusters, the disease index and the control effect of disease fingers were 6.00%, 80.52%, 2.91 and 82.50% respectively, all of which were higher than those in other treatment groups. The yield results indicated that the yield of wheat treated with 19% picoxystrobin · propiconazole microemulsion was 7 146.60 kg/hm², which was higher than that of other treatments. Compared with CK, the yield increase rate was 11.39%. The comprehensive results show that under the conditions of plant protection unmanned aerial vehicles, the application of 19% picoxystrobin · propiconazole microemulsion to control wheat scab has a good control effect, a long persistence period, and a certain yield-increasing effect.

Irrigation methods and nitrogen (N) fertilization modes have a complicated impact on wheat physiology, growth, and development, leading to the regulation of wheat grain yield and quality.  However, the optional water-N combination for drip-irrigated winter wheat remains unclear.  A two-year fieldwork was conducted to evaluate the influences of various N-fertigation and water regimes on wheat post-anthesis grain weight variation, yield, grain NPK content, and grain quality.  The two irrigation quotas were I₄₅ (Irrigation when ETa-P reaches 45 mm) and I₃₀ (Irrigation when ETa-P reaches 30 mm) and the six N application rates were N_0-100 (100% at jointing/booting), N_25-75 (25% at sowing and 75% at jointing/booting), N_50-50 (50% at sowing and 50% at jointing/booting), N_75-25 (75% at sowing and 25% at jointing/booting), N_100-0 (100% at sowing), and SRF₁₀₀ (100% of slow release fertilizer at sowing).  The experimental findings showed that post-anthesis grain weight variation, grain yield, grain NPK content, and grain quality were markedly influenced by the various irrigation scheduling and N-fertilization modes.  The N_50-50 treatment was more beneficial for winter wheat post-anthesis grain weight variation than the N_100-0 and N_0-100 treatments under the two irrigation quotas and during the two seasons.  The highest grain yield of 9.72 and 9.94 (t ha⁻¹) were obtained with the I₄₅N_50-50 treatment in 2020-2021 and 2021-2022, respectively.  The grain crude protein was higher in the I₄₅SRF₁₀₀ treatment during the two seasons.  The I₄₅N_100-0 significantly (P<0.05) enhanced the content of grain total starch by 7.30 and 8.23% compared with the I₄₅N_0-100 and I₃₀N_0-100 treatments, respectively during the 2021-2021 season.  The I₄₅N_100-0 significantly (P<0.05) enhanced the content of grain total starch concentration by 7.77%, 7.62 and 7.88% in comparison with the I₄₅N_0-100, I₃₀N_0-100, and I₃₀N_25-75 treatments, respectively in the 2021-2022 season. Considering the principal component analysis (PCL), the N_50-50 split N-fertigation mode could be an optional choice for farmers during winter wheat production via drip irrigation.

Fusarium graminearum is a fungal plant pathogen which causes Fusarium head blight (FHB), a devastating disease on cereal crops. Here we report that FgPMA1 could be a new target to control FHB by the application of double-stranded RNA (dsRNA) of FgPMA1. FgPMA1 was divided into 6 segments to generated RNA interference (RNAi) constructs (FgPMA1RNAi-1, -2, -3, -4, -5, and -6), and these constructs were transformed in F. graminearum strain PH-1. The expression of FgPMA1 reduced by 18.48%, 33.48% and 56.93% in FgPMA1RNAi-1, FgPMA1RNAi-2 and FgPMA1RNAi-5, respectively. FgPMA1RNAi-1, -2, and -5 mutants inhibited fungal development, including mycelium growth, mycelial morphology, asexual and sexual development, and toxin production. The length of lesions on wheat leaves, wheat coleoptiles and wheat ears were shorter after infection with FgPMA1RNAi-1, -2, and -5 mutants than wild-type PH-1. These results showed that three segments (FgPMA1RNAi-1, -2, and -5) exhibited effective silencing effects. After treatment with 25 ng µL^-1 dsRNA of these segments in vitro, the growth rate of mycelium growth was significant decreased, mycelium became deformed with bulbous structure at the tip, and the mycelium lost the ability to produce conidia in F. graminearum strain PH-1, Fusarium asiacitum strain 2021 and phenamacril-resistant strain YP-1. After application of FgPMA1RNAi-1-dsRNA and FgPMA1RNAi-2-dsRNA to wheat ears, pathogenicity reduced 34.21-35.40%. 

Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is an airborne disease. In China, it frequently develops initially in central Shaanxi and southwestern Gansu, and from there, inoculum spreads to the eastern wheat production regions. Field investigations have suggested that Pst could spread from the west to the east within central Shaanxi and that Gansu could serve as the inoculum source for central Shaanxi, but there is no direct evidence for this hypothetical dispersal route. In the current study, 321 Pst isolates collected from central Shaanxi and Gansu in the 2019-2020 and 2020-2021 winter wheat cropping seasons were genotyped using 23 pairs of KASP-SNP markers. The dispersion among subpopulations was analyzed using several approaches, and overall, the populations were found to exhibit high levels of genetic diversity. There was little genetic divergence (0.05 > F_st > 0) within central Shaanxi. However, significant gene flow (Nm > 4) driven by wind-oriented dispersal from west (Baoji) to east (Weinan) occurred. There was also gene flow among the 4 Gansu subpopulations of Tianshui, Longnan, Pingliang, and Qingyang. Migration of the pathogen occurred between central Shaanxi and Gansu. Migration from Gansu to central Shaanxi was major compared with that from central Shaanxi to Gansu that was minor. Genetic variation occurred among isolates, instead of among subpopulations and within isolates. Linkage disequilibrium revealed that there was strong genetic recombination in the subpopulations from Gansu and central Shaanxi. Therefore, the present study provides molecular evidence that Pst spread from west to east in central Shaanxi and showed that Gansu (especially Longnan and Tianshui) was one of the major origins of the pathogen inoculum of wheat stripe rust in central Shaanxi. The results revealed the west-to-east transmission route of wheat stripe rust in central Shaanxi, being used to guide integrated management of the disease. 

【Objective】 Wheat is a cornerstone of global food security, with its production being pivotal in both China and the international community. With global climate change, the threat of high temperature has become increasingly prominent, posing a significant challenge to wheat cultivation. The strategic identification and selection of heat-tolerant germplasm, coupled with the exploration of genes associated with heat resistance, are crucial steps. These efforts are essential for broadening the genetic diversity of heat tolerance in wheat within China, providing prerequisites for breeding heat-tolerant wheat varieties and ultimately contributing to the safeguarding of our nation’s food security in the face of a warming climate. 【Method】 In this study, a natural population of 331 wheat accessions was utilized, and artificial climate chambers were employed to simulate high temperatures conditions. The heat tolerance of wheat seedlings was assessed by monitoring their survival rate under various durations of treatment, using heat resistance grade as the evaluative metric. Meanwhile, a genome-wide association study (GWAS) was conducted using the 55K SNP chip to identify genetic loci associated with heat tolerance. Expression data from multiple tissues, including roots, leaves under heat stress were analyzed, leading to the selection of genes related to heat tolerance. Subsequently, qPCR validation of candidate genes was performed using the extremely heat-tolerant accession Xinong 889 and the heat-sensitive accession Chinese Spring (CS) as materials. 【Result】 Under high-temperature stress, significant variations in survival rates were observed among different wheat accessions. The extremely heat-tolerant, moderately heat-tolerant, moderately heat-sensitive, and extremely heat-sensitive germplasm accounted for 110, 104, 110, and 7, respectively, representing 33.23%, 31.42%, 33.23%, and 2.12% of the total. Heat-tolerant germplasms, including Xinong 889, Zhengmai 7698, Zhongmai 895, Zhoumai 18, and Fengchan 3, were identified. Through GWAS, a total of 293 SNP loci significantly associated with the 12-hour survival rates (SR) and heat resistance grades (HRG) were detected, with the phenotypic variation explained ranging from 4.40% to 12.46%. Among these, 200 loci were related to the 12-hour survival rates, and 257 were related to the heat resistance grades, with 164 loci identified as the same heat-related loci. Based on significantly associated SNP markers, 313 heat-related genes were predicted. According to gene annotation information and expression data under heat stress, 23 heat tolerance candidates were selected, and after qPCR validation of differentially expressed candidate’s genes, 20 key heat tolerance candidate genes were identified. 【Conclusion】 At the seedling stage, 331 wheat germplasms were identified for heat tolerance. A rapid method was developed for determining the survival rate of wheat seedlings subjected to treatments of varying durations at 45 ℃ to assess their heat tolerance In total, 38 heat-tolerant germplasms and 293 loci significantly associated with seedling heat tolerance were screened. Also, TraesCS1A02G355900, TraesCS1A02G389500, TraesCS5A02G550700, TraesCS5D02G557100, TraesCS6D02G402500 and TraesCS7A02G232500 represented as candidate genes were filtered out.

Wheat is one of the important food crops. Due to global warming and changes in farming patterns, wheat planting and production processes are facing frequent diseases, insects and weeds damage, which pose a major threat to wheat yield and quality. Plant growth regulators, as substances that control plant growth and improve yield and quality, have been widely used in crop production. However, there are relatively few studies on the application of plant growth regulators in wheat production through basal application. To explore the effects of different plant growth regulators on wheat seed germination, seedling growth and yield, this study selected three plant growth regulators of mepiquat chloride, choline chloride and diethyl aminoethyl hexanoate, and used the basal application method to study their effects on wheat. The results showed that the three plant growth regulators had significant (P≤0.05) effects on the germination potential and germination rate of wheat seeds, and had different promotion effects on the growth of wheat seedlings. When wheat was treated with 30 mg/L mepiquat chloride, 20 mg/L choline chloride and 4 mg/L diethyl aminoethyl hexanoate, plant height, root length, stem and leaf fresh weight and root fresh weight were significantly increased. Meanwhile, in the field experiment, the results showed that the treatment of wheat with 75 g (ai)/hm² mepiquat chloride, 30 g (ai)/hm² choline chloride and 30 g (ai)/hm² diethyl aminoethyl hexanoate had a significant effect on wheat seedlings, and the three plant growth regulators had different degrees of improvement on wheat yield.

To screen for efficient and safe pre-emergence soil-applied herbicides suitable for winter wheat fields, an evaluation was conducted on the weed control efficacy and safety of 6 herbicides including 33.8% oxadiazon·flufenacet SC through soil sealing treatment. The results showed that 33.8% oxadiazon·flufenacet SC at 750 mL/hm² demonstrated optimal soil-sealing efficacy in wheat fields, achieving 83.29% weed control efficiency 28 days after treatment. Following subsequent foliar application, it maintained 98.74% weed control rate and 95.09% fresh weight reduction efficacy 98 days post-treatment, significantly reducing early-stage weed populations with prolonged residual activity and good crop safety. 50% pretilachlor SC at 1 875 mL/hm² achieved weed control 82.71% at 28 days, but shorter persistence, with fresh weight control declining to 72.52% at 98 days after foliar treatment. 30% pyroxasulfone·flufenacet SC at 1 200 mL/hm² achieved 78.33% weed control at 28 days, exhibiting longer persistence with 93.66% fresh weight control efficacy at 98 days post foliar treatment. These findings validate that 3 herbicides can be effectively applied as pre-emergence soil treatments in winter wheat fields, demonstrating significant weed suppression without observable phytotoxicity to wheat growth. They exhibit promising potential for extended application in winter wheat cultivation regions such as Shanghai. Rotational use is recommended according to local weed infestation patterns to optimize resistance management and sustained efficacy.

This study aims to screen biocontrol strains against Fusarium graminearum species complex in order to provide new biocontrol resources for the sustainable management of fusarium head blight. A high-efficiency biocontrol strain was isolated from non-agricultural soil samples collected in Badong County, Enshi, Hubei Province, using the dilution plate method with Fusarium graminearum as the indicator strain. The inhibitory effects of the isolate Glarea lozoyensis fermentation broth on mycelial growth and spore germination of F. graminearum were assessed,as well as its degradation rates on deoxynivalenol (DON) and zearalenone (ZEN) in vitro. The strain was identified through morphological characteristics and 18S rRNA gene sequence analysis. Further experiments were conducted to assess control efficacy in field plots. The results showed that a 10-fold dilution of its fermentation broth inhibited F. graminearum mycelial growth and spore germination by 91.98% and 100%, respectively, and achieved 100% degradation rates for DON and ZEN. Morphological characteristics and 18S rRNA gene analysis identified as Glarea lozoyensis (GL), designated GL-P1. Field trial results demonstrated that the fermentation broth of GL-P1 achieved a 71.36% control efficacy against Fusarium head blight, comparable to the fungicide tebuconazole. The above study indicates that Glarea lozoyensis P1 is a biocontrol isolate with promising field efficacy against Fusarium head blight, showing potential for further application and development.

To study the performance of yield and quality traits of wheat germplasm resources and their correlation and path analysis，35 wheat germplasm resources from the middle and lower reaches of the Yangtze River were used as materials in this study. The plant height, spike number, spikelet number per spike, 1000-grain weight and yield were investigated manually, and the wheat quality was determined by Perten DA 7200 near-infrared analyzer. The results showed that the yield range of 35 wheat germplasm resources was 5679.00-6331.50 kg/hm², the effective spike range was 427.50-502.50 million/hm², the growth period range was 195.00-202.00 d, the plant height range was 73.00-93.00 cm, the spikelet number per spike range was 32.60-40.00, and the 1000-grain weight range was 37.00-47.10 g. The correlation analysis of yield traits showed that effective spike, growth period, spikelet number per spike and 1000-grain weight were positively correlated with grain yield (r=0.1707, r=0.0401, r=0.2277, r=0.0299), while plant height was negatively correlated with grain yield (r=-0.0587). Path analysis showed that grain yield increased while effective spike, spikelet number per spike and 1000-grain weight increased. The effect of increasing the number of grains per ear was the greatest (direct path coefficient Py=0.8390), followed by the effect of increasing the 1000-grain weight (direct path coefficient Py=0.6668), and the effect of increasing the number of effective ears was the smallest (direct path coefficient Py=0.4916). In general, the quality traits of 35 wheat germplasm resources were quite different, indicating that wheat has rich diversity, which provides a large selection space for wheat quality breeding and selection of offspring.

叶锈病是危害小麦生产的主要病害之一，栽培小麦广谱高抗叶锈病基因匮乏。小麦-冰草易位系2PT-5具有来自冰草2P长臂对小麦叶锈病广谱免疫的区段。为了准确定位该抗叶锈病基因区段，本研究利用辐照诱导获得的小麦-冰草2P易位系TT-5、TT-3和TT-26分离群体进行叶锈菌接种鉴定，结合基因组原位杂交（GISH）、分子标记检测和基因组重测序对抗叶锈病基因进行物理定位。将抗叶锈病定位区间由原来的82 Mb缩小至9.2 Mb，定位于2P长臂物理位置926.4~935.6 Mb区间。目标区间内注释了64个冰草特异基因，包含6个典型抗病基因，其中有2个编码NLR蛋白的基因和2个编码受体激酶的基因响应叶锈菌的侵染。抗叶锈病基因目标区段的定位，为进一步克隆和解析转移到小麦中的这一广谱抗叶锈病基因奠定了重要的基础。

To accelerate the high quality production of wheat in Hexian, Anhui Province, suitable wheat varieties for local planting were selected, including 10 varieties such as Ningmai 13 and Zhenmai 15, for demonstration experiments. Ningmai 13 was used as a control to comprehensively investigate the growth period, agronomic traits, morphological resistance, and yield characteristics of each variety. The results indicated that the entire growth period of each variety was between 200-212 days. In terms of agronomic traits, the plant height ranges from 73.3 to 87.4 cm, the ear length ranges from 6.8 to 10.4 cm, the number of grains per ear ranges from 33.6 to 39.8, the thousand grain weight ranges from 36.1 to 43.8 g, and the effective ear count ranges from 4.245 million to 5.775 million ears per hectare. In terms of resistance, 7 varieties including Ningmai 24, Ningmai 26, and Zhenmai 12 had strong lodging resistance, while Zhenmai 18, Yangmai 34, and Zhenmai 15 had better resistance to Fusarium head blight. The top 3 wheat varieties in terms of yield are Zhenmai 18 (6 789.0 kg/hm²), Yangmai 34 (6 762.0 kg/hm²), and Zhenmai 15 (6 724.5 kg/hm²), which increased yield by 12.5% to 13.5% compared to the CK. Based on the comprehensive analysis of the growth period, agronomic traits, resistance, and yield performance of various varieties, Zhenmai 18, Yangmai 34, and Zhenmai 15 were selected to perform well under the climatic conditions of the experimental field in that year.

[Objective] Plant leaf shape is an important part of plant architectural model. Establishment of a three-dimensional structural model of leaves may assist simulating and analyzing plant growth. However, existing leaf modeling approaches lack interpretability, invertibility, and operability, which limit the estimation of model parameters, the simulation of leaf shape, the analysis and interpretation of leaf physiology and growth state, and model reusage. Aiming at the interoperability between three-dimensional structure representation and mathematical model parameters, this study paid attention to three aspects in wheat leaf shape parametric reconstruction: (1) parameter-driven model structure, (2) model parameter inversion, and (3) parameter dynamic mapping during growth. Based on this, a set of parameter-driven and point cloud inversion model for wheat leaf interoperability was proposed in this study. [Methods] A parametric surface model of a wheat leaf with seven characteristic parameters by using parametric modeling technology was built, and the forward parametric construction of the wheat leaf structure was realized. Three parameters, maximum leaf width, leaf length, and leaf shape factor, were used to describe the basic shape of the blade on the leaf plane. On this basis, two parameters, namely the angle between stems and leaves and the curvature degree, were introduced to describe the bending characteristics of the main vein of the blade in the three-dimensional space. Two parameters, namely the twist angle around the axis and the twist deviation angle around the axis, were introduced to represent the twisted structure of the leaf blade along the vein. The reverse parameter estimation module was built according to the surface model. The point cloud was divided by the uniform segmentation method along the Y-axis, and the veins were fit by a least squares regression method. Then, the point cloud was re-segmented according to the fit vein curve. Subsequently, the rotation angle was precisely determined through the segment-wise transform estimation method, with all parameters being optimally fit using the RANSAC regression algorithm. To validate the reliability of the proposed methodology, a set of sample parameters was randomly generated, from which corresponding sample point clouds were synthesized. These sample point clouds were then subjected to estimation using the described method. Then error analyzing was carried out on the estimation results. Three-dimensional imaging technology was used to collect the point clouds of Zhengmai 136, Yangmai 34, and Yanmai 1 samples. After noise reduction and coordinate registration, the model parameters were inverted and estimated, and the reconstructed point clouds were produced using the parametric model. The reconstruction error was validated by calculating the dissimilarity, represented by the Chamfer Distance, between the reconstructed point cloud and the measured point cloud. [Results and Discussions] The model could effectively reconstruct wheat leaves, and the average deviation of point cloud based parametric reconstruction results was about 1.2 mm, which had a high precision. Parametric modeling technology based on prior knowledge and point cloud fitting technology based on posterior data was integrated in this study to construct a digital twin model of specific species at the 3D structural level. Although some of the detailed characteristics of the leaves were moderately simplified, the geometric shape of the leaves could be highly restored with only a few parameters. This method was not only simple, direct and efficient, but also had more explicit geometric meaning of the obtained parameters, and was both editable and interpretable. In addition, the practice of using only tools such as rulers to measure individual characteristic parameters of plant organs in traditional research was abandoned in this study. High-precision point cloud acquisition technology was adopted to obtain three-dimensional data of wheat leaves, and pre-processing work such as point cloud registration, segmentation, and annotation was completed, laying a data foundation for subsequent research. [Conclusions] There is interoperability between the reconstructed model and the point cloud, and the parameters of the model can be flexibly adjusted to generate leaf clusters with similar shapes. The inversion parameters have high interpretability and can be used for consistent and continuous estimation of point cloud time series. This research is of great value to the simulation analysis and digital twinning of wheat leaves.

Multiple planting of soybeans after wheat harvest can fully utilize the remaining light, hot, and water resources after wheat harvest, achieve two crop intercropping in one year. The planting practice was combined of wheat and soybean in Shangluo, Shaanxi Province, the light, simple, and efficient cultivation techniques for winter wheat and high yield cultivation techniques for multiple cropping of soybeans were summarized and analyzed, at the same time, corresponding yield improvement strategies were proposed by focusing on the key aspects of intercropping soybeans after wheat. The cultivation techniques for light, simple, and efficient winter wheat include selecting high quality, high yielding, disease resistant, and early maturing wheat varieties; before sowing, rotary tiller and rake flat, and apply appropriate amount of base fertilizer; reasonably determine the broadcasting schedule and quantity; check and supplement seedlings, and timely carry out “one spray and three prevention” measures; timely harvest and clean up residual debris. The high yield cultivation techniques for multiple cropping of soybean varieties include selecting early maturing varieties with strong stress resistance and high and stable yield; plow and weed before sowing, and apply appropriate amount of base fertilizer; timely sowing of moisture and reasonable planting density; combining tillage weeding with chemical weeding, applying fertilizer while the soil is moist, and implementing rational control measures; seize the critical window period for disease and pest control and implement comprehensive prevention and control measures; remove from drying and store in the warehouse. The key points to focus on in the research area for wheat multiple cropping soybean include wheat harvest period, planting efficiency, soil preparation quality, and mechanization level. Therefore, reasonable crop rotation and good crop rotation connection were proposed; promote high yield cultivation techniques, and improve planting efficiency; strategies include fine soil preparation, improving soil fertility, introducing new machinery, and expanding the area of intercropping. This article provides a reference for achieving high yields by multiple cropping soybeans after wheat in Shangluo and related areas.

To explore the comprehensive effects of liquid nitrogen fertilizer on wheat growth characteristics, yield, and its soil nutrients, urea ammonium nitrate solution (UAN) was used to replace urea in equal amounts, and compared with the conventional application of urea (CK), wheat agronomic traits such as total stem number, yield components such as panicle number, soil nutrients such as alkali-hydrochloric nitrogen, nitrogen partial productivity, etc were measured. The results showed that the total stem number, leaf area index and flag leaf SPAD of UAN increased by 31.9%, 9.9%, and 2.4%～3.5% compared with CK, respectively. Compared with CK, panicle number, grain number per spike, thousand grain weight, and yield were increased by 19.8%, 0.9%, 1.0%, and 22.2%, respectively. UAN could increase the nitrogen partial productivity of wheat by 99.2% compared with CK. The content of soil available nutrients after application of UAN was generally higher than CK. Compared with CK, wheat output value and yield increased by 22.2% and 89.1%, respectively. In conclusion, the application of UAN can promote the growth and development of wheat, increase the yield, improve the physical and chemical properties of soil, reduce the amount of nitrogen fertilizer and increase the nitrogen partial productivity, with better economic and ecological benefits.

To explore the effect of alginate enhancing solution on the photosynthetic characteristics and yield of winter wheat, four different concentration treatments were set during the regreening stage, jointing stage and booting stage of winter wheat, which were 0 mg/kg (CK), 30 mg/kg (N30), 60 mg/kg (N60) and 90 mg/kg (N90). The indexes such as chlorophyll content, grain filling rate, dry matter accumulation and distribution, photosynthetic characteristics and yield among different treatments were studied. The results showed that spraying alginate enhancing solution could significantly increase the chlorophyll content of wheat flag leaves and the grain filling rate, especially in the late stage of flowering, it still maintained a significant advantage compared with CK. The dry matter accumulation of stem sheath + leaves, ear shaft + glume, grain and distribution ratio were significantly higher than those of CK, among which the advantage of N30 treatment at the jointing stage was the most obvious. In photosynthesis, spraying alginate enhancing solution significantly increased the net photosynthetic rate (Pn), stomatal conductance (Gs) and transpiration rate (Tr) of flag leaves, reduced the intercellular carbon dioxide concentration (Ci) of flag leaves, keeping the crops at a high photosynthetic rate all the time. In terms of yield and composition factors, there were no significant differences in the number of spikes and grains per spike among different concentration treatments compared with CK, but the thousand grain weight and yield were significantly higher than CK. Among them, spraying alginate enhancing solution at 30 mg/kg at the jointing stage had the best yield-increasing effect, with a yield of 8811.96 kg/hm², which was 12.01% higher than that of the CK treatment in the same growth period. In summary, spraying alginate enhancing solution at 30 mg/kg at the jointing stage is a more reasonable fertilization scheme.

To further understand the production characteristics and applicable value of the wheat variety ‘Hemai 26’, its yielding ability, yielding stability and adaptability as well as resistibility and quality characteristic were analyzed with ‘Zhoumai 18’ as the contrast using the regional tests of wheat varieties in southern Huang-Huai Wheat Region consisted of institutions and enterprises during 2016-2018 and production tests in 2018-2019. The results showed that the yield of ‘Hemai 26’ increased by 4.5%, 11.53% and 5.28% respectively compared with that of ‘Zhoumai 18’ during 2016-2019, and reached a significant level. Coefficient of variation (CV) of ‘Hemai 26’ was slightly smaller, the high stability coefficient (HSC) was larger and adaptability was higher than that of ‘Zhoumai 18’. The average yield of each experimental variety showed linear regression with the average yield of ‘Hemai 26’ in different test sites, and the regression coefficient tended to 1. ‘Hemai 26’ has strong lodging resistance and general disease resistance. ‘Hemai 26’ has good grain uniformity, high grain bulk density and good quality. ‘Hemai 26’ has outstanding yielding ability, good yielding stability, wide adaptability and excellent quality, which has good promotion and utilization value in Southern Huang-Huai Wheat Region.

【Objective】 Common wheat, as an important food crop, plays a crucial role in global food security. Identifying the gene regulatory networks involved in wheat grain protein synthesis and determining key candidate genes will provide theoretical support for quality breeding and improvement of wheat. 【Method】 The study used wheat grains at six developmental stages (5, 10, 15, 20, 25, and 30 days post-anthesis) as research materials to summarize the pattern of protein accumulation in wheat grains. Transcriptome data and grain protein content phenotypic data were analyzed using the WGCNA (Weighted Gene Co-expression Network Analysis) method to construct weighted gene co-expression networks and identify key hub transcription factor (TFs) genes.【Result】 The accumulation of protein content in wheat grains showed a trend of initial decline followed by an increase, reaching its lowest value (12.16%) at 25 days post-anthesis, with significant differences in protein content between adjacent developmental stages. A total of 25 427 differentially expressed genes (DEGs) were identified between adjacent developmental stages. Cluster analysis divided these DEGs into five groups (A-E), with group B containing the highest number of DEGs (10 906). A total of 1 022 transcription factors (TFs) from 49 families were identified, with the NAC family containing the most TFs (107). WGCNA analysis identified five co-expression modules significantly associated with protein content. The turquoise module showed the highest positive correlation with protein content (r=0.80, P=1×10^-⁴). By integrating differentially expressed genes and weighted gene co-expression networks, six positively regulated hub TFs from the MIKC-MADS, TCP, TALE, and CPP families were identified in two modules (turquoise and blue). Further correlation analysis between the protein content phenotype of Huaimai 48 and gene expression levels at different time points revealed that the expression levels of five hub TFs were significantly positively correlated with the protein content phenotype. Specifically, TraesCS5B03G0740100 and TraesCS7D03G0590500 showed specific high expression in spike and grain tissues.【Conclusion】 The study identified important modules (turquoise and blue) related to wheat protein content accumulation, screened six hub TFs, and identified that the expression levels of two hub TFs are significantly positively correlated with protein content and are specifically highly expressed in spike and grain tissues. These genes can serve as candidate genes for regulating protein accumulation in wheat grains.

【Objective】 Under the background of global warming, this paper explored the physiological mechanism of anthocyanin content in colored wheat in response to high temperature stress in the middle of grain filling, so as to lay a theoretical basis for further coping with the high-quality cultivation of functional colored wheat varieties under climate warming. 【Method】 The experiment was conducted in Hefei High-tech Agricultural Park in the 2022-2023 and 2023-2024 growing seasons. Six colored wheat varieties with different colors were selected and subjected to high temperature stress treatment (T) for 5 days at the middle stage of filling, with the same materials grown under ambient temperature as the controls (CK). 【Result】 Under high temperature stress after anthesis, the net photosynthetic rate, stomatal conductance, transpiration rate, relative chlorophyll content (SPAD), dry matter partition, soluble sugar content, sucrose synthase activity, anthocyanin content, anthocyanidin synthase activity, chalcone synthase activity and phenylalanine ammonia-lyase activity of colored wheat were significantly reduced, and the yield of six varieties of colored wheat decreased by 9.10% to 16.94%, 1000-grain weight decreased by 7.84% to 16.94%, and anthocyanin content decreased by 7.18% to 14.17%. The yield, photosynthetic intensity, SPAD value, dry matter partition, soluble sugar content, sucrose synthase activity, anthocyanin content, anthocyanidin synthase activity, chalcone synthase activity, and phenylalanine ammonia-lyase activity of different varieties of colored wheat were: Qinbai 1>Qinlü 3>Qinzi 1>Xinchun 36>Qinhe 2>Qinlan 1, and the anthocyanin content was: Qinhei 2>Xinchun 36>Qinzi 1>Qinlü 3>Qinlan 1>Qinbai 1. The yield of heat-resistant wheat varieties of Qinbai 1, Qinlü 3 and Qinzi 1 decreased significantly less than that of heat-sensitive wheat varieties Qinhei 2, Xinchun 36 and Qinlan 1. The decreases in photosynthetic intensity, SPAD value, dry matter fraction, soluble sugar content, sucrose synthase activity, anthocyanin content, anthocyanidin synthase activity, chalcone synthase activity and phenylalanine ammonia-lyase activity of color wheat varieties Qinhei 2, Xinchun 36 and Qinzi 1 with high anthocyanin content were smaller than those of Qinlü 3, Qinlan 1 and Qinbai 1 with low anthocyanin content. Correlation analysis showed that the yield of each color wheat variety was significantly positively correlated with 1000-grain weight, sucrose content, sucrose synthase activity, flag leaf net photosynthetic rate, stomatal conductance, transpiration rate and SPAD value, anthocyanin content was significantly positively correlated with soluble sugar content, and yield was negatively correlated with anthocyanins, but the correlation was not significant. After high temperature stress after anthesis, the decomposition of sucrose bound to free anthocyanins decreased, and the decomposition of anthocyanins in grains increased, which supplemented the growth and development of wheat.【Conclusion】 The antioxidant activity of anthocyanins helped crops resist external stress, and the decline of various indexes of color wheat varieties with higher anthocyanin content was comparable to that of color wheat varieties with lower anthocyanin content under high temperature stress after anthesis, the anthocyanin content was significantly positively correlated with soluble sugar content. In conclusion, the accumulation of anthocyanin content could respond to high temperature stress, reduce the decrease of soluble sugar content, and increase the heat resistance of colored wheat.

Determining the safety threshold of heavy metals in soil is an important measure to ensure the quality and safety of agricultural products in China. By studying the accumulation and transportation characteristics of five heavy metals (Cr, Cd, Pb, As, and Hg) in different organs of corn and wheat in the cities of Jinchang, Zhangye and Baiyin in Gansu Province and establishing the relationship between the effective content of Cr, Cd, Pb, As, and Hg in soil and crop grains, this study identified the safety threshold of effective heavy metals in soil for corn and wheat systems. This study employed the method and principles of species sensitivity distribution (SSD) and derived the safety threshold of effective heavy metals in soil for corn and wheat planting systems based on the cumulative probability distribution curve of the Logistic function distribution model. The results showed that the accumulation pattern of Cr, Cd, Pb, As and Hg in wheat plants was consistent, namely, roots>stems>grains, and similarly, the accumulation pattern of Cr, Pb, As and Hg in corn plants was roots>stems>grains, while the accumulation pattern of Cd was stems>roots>grains. Utilizing the Logistic function distribution model to fit the cumulative probability distribution curve based on the effective content of heavy metals, the safety thresholds of effective Cr, Cd, Pb, As and Hg in wheat soil were determined to be 0.019, 0.771, 35.294, 2.777 and 0.133 mg/kg, respectively. Meanwhile, the safety thresholds of effective Cr, Cd, Pb, As and Hg in corn soil were determined to be 0.296, 7.90, 52.363, 12.462 and 0.119 mg/kg, respectively. The results of this study indicate that estimating the safe threshold of effective heavy metal content based on the cumulative probability distribution curve method was scientific, providing a scientific basis and support for the safe planting and risk control of wheat and corn.

This study explores the changes in nitrogen application rate and the effects of increased CO₂ concentration on wheat yield and biomass in semi-arid areas under different sowing dates. This article used the adjusted APSIM model to set up three different sowing dates of early sowing ESW (March 3rd), normal sowing NSW (March 19th), and late sowing LSW (March 31st), five nitrogen application treatments of N1(0 kg/hm²), N2(52.5 kg/hm²), N3(105 kg/hm²), N4(157.5 kg/hm²), and N5 (210 kg/hm²), and five CO₂ concentrations of C1 (370 ppm), C2 (420 ppm), C3 (470 ppm), C4 (520 ppm), and C5 (570 ppm) for simulation experiments. The results indicated that the APSIM model had good applicability in the experimental area; both nitrogen application rate and CO₂ concentration had an impact on the yield and biomass of dryland spring wheat, and the effects varied under different sowing dates. As the sowing date was delayed, both nitrogen application rate and CO₂ concentration had a positive effect on wheat yield and biomass, with nitrogen application rate having a much greater impact than CO₂ concentration; under specific nitrogen application rates and CO₂ concentrations, the choice of sowing date had a significant impact on yield and biomass. Higher yields were typically achieved during normal sowing periods, while late sowing was beneficial for biomass accumulation. When climate and soil conditions change, adjusting the sowing date can optimize wheat production. When the nitrogen application rate was N4 and the CO₂ concentration was C1, the wheat yield was highest under normal sowing, and the wheat biomass was highest under late sowing. This study holds significant importance in guiding wheat production in semi-arid regions. In practical production, based on local climate and soil conditions, parameters such as sowing dates, nitrogen application rates, and CO₂ concentrations can be adjusted to optimize wheat production and enhance both yield and biomass. Especially in the context of global climate change and the numerous challenges faced by agricultural production, this research provides robust support for the sustainable development of wheat production in semi-arid areas.

To examine the impact of compound oligosaccharides on crop yield in Shandong, we conducted an experiment with various treatments, including mixing wheat seeds with compound oligosaccharides and spraying compound oligosaccharides at different stages, as well as soaking corn seeds and spraying with compound oligosaccharides at different stages. In Daiyue District, Shandong Province, wheat spraying at the flowering + regreening period under seed-mixing conditions exhibited superior efficacy compared to spraying solely at the flowering or regreening period, but the difference was not significant. In Huantai County, under seed-mixing conditions, spraying at the regreening period, flowering period, and regreening + flowering period outperformed seed-mixing treatment, with the most favorable outcome observed with seed-mixing + spraying at the flowering period. Conversely, without seed mixing, spraying at the regreening period, flowering period, regreening + flowering period all surpassed the control, with the highest yield increase achieved through spraying at the flowering period. Spray treatment of compound oligosaccharides resulted in higher yields compared to non-soaking methods, with Daiyue District and Huantai County achieving the highest yield and economic benefit during the flowering + filling period and filling period, respectively. When seeds were soaked, the combination of spray treatment during the flowering and filling period yielded the best results in Daiyue District, while the seedling and filling period were most effective in Huantai County. Compound oligosaccharides can substantially enhance crop yield and economic returns. In this region, wheat benefits from seed mixing with compound oligosaccharides combined with spraying at the flowering stage, while maize shows improved outcomes with spraying during the seedling or flowering + filling period, following seed soaking treatment.

Early diagnosis and comprehensive prevention and control techniques were explored for wheat disease, and strategies to improve the effectiveness of wheat disease prevention and control were proposed. Early diagnosis techniques include disease symptom recognition techniques that rely on disease symptom maps, deep learning algorithms, and remote sensing technology, and molecular biology techniques such as polymerase chain reaction and gene chips. Comprehensive prevention and control techniques include establishing and applying disease prediction models based on meteorological data, as well as disease prediction and warning systems; applying reasonable crop rotation systems, optimizing sowing time and density, and other agricultural operation techniques. The strategies to improve the effectiveness of wheat disease prevention and control include the research and promotion of new diagnostic technologies such as nanotechnology and biosensors, as well as the integration and application of comprehensive prevention and control technologies such as agricultural control, physical control, and chemical control, to promote the innovation of diagnosis and control technologies; the measures such as conducting on-site demonstrations, remote teaching, and organizing experience exchange meetings, and establishing demonstration bases to strengthen farmer education and technical training. The application of relevant techniques provides a reference for improving the scientific and timely prevention and control of wheat disease.

The wheat above-ground biomass (AGB) is an important index that shows the life activity of vegetation, which is of great significance for wheat growth monitoring and yield prediction.  Traditional biomass estimation methods specifically include sample surveys and harvesting statistics.  Although these methods have high estimation accuracy, they are time-consuming, destructive, and difficult to implement to monitor the biomass at a large scale.  The main objective of this study is to optimize the traditional remote sensing methods to estimate the wheat AGB based on improved convolutional features (CFs).  Low-cost unmanned aerial vehicles (UAV) were used as the main data acquisition equipment.  This study acquired RGB and multi-spectral (MS) image data of the wheat population canopy for two wheat varieties and five key growth stages.  Then, field measurements were conducted to obtain the actual wheat biomass data for validation.  Based on the remote sensing indices (RSIs), structural features (SFs), and convolutional features (CFs), this study proposed a new feature named AUR-50 (Multi-source combination based on convolutional feature optimization) to estimate the wheat AGB.  The results show that AUR-50 could more accurately estimate the wheat AGB than RSIs and SFs, and the average R² exceeded 0.77.  AUR-50_MS had the highest estimation accuracy (R² of 0.88) in the overwintering period.  In addition, AUR-50 reduced the effect of the vegetation index saturation on the biomass estimation accuracy by adding CFs, where the highest R² was 0.69 at the flowering stage.  The results of this study provide an effective method to evaluate the AGB in wheat with high throughput and a research reference for the phenotypic parameters of other crops.

【Objective】This study aimed to investigate the impact of wheat bran polysaccharide on the physical properties and microstructure of surimi gel, and to elucidate the mechanism by which the concentration of wheat bran polysaccharide affects surimi gel characteristics, so as to provide the theoretical support for enhancing surimi gel quality through the utilization of wheat bran by-products.【Method】Different concentrations of wheat bran polysaccharide were added to threadfin bream (Nemipterus virgatus) surimi to prepare composite gels. The effects of wheat bran polysaccharide on the characteristics and structures of surimi gel were investigated using texture analysis, rheological test, molecular interaction measurement, Fourier transform infrared (FTIR) spectroscopy, and scanning electron microscopy (SEM).【Result】The impact of wheat bran polysaccharide on the macroscopic physical properties and microstructures of surimi gel exhibited a pronounced concentration dependence. As the amount of wheat bran polysaccharide increased, the rheological parameters (G' and G" values), water holding capacity, texture profile analysis (TPA) values, gel strength, and water distributions of the blended system were all initially elevated and then declined, achieving the maximum improvement at the addition level of 1.0%. FTIR revealed that the secondary structure of proteins during the heat-induced gelation of surimi was closely related to the polysaccharide content: with increasing polysaccharide content, the α-helix content gradually decreased, while β-turn and β-sheet content showed an upward trend (P<0.05). However, when the polysaccharide addition exceeded 1.0%, the α-helix content gradually increased again, while β-turn and β-sheet content exhibited downtrends. This may be due to the concentration-dependent effect of polysaccharides on the disulfide bonds and electrostatic interactions, which were the primary intermolecular forces within the protein gel, leading to the changes in its secondary structure. SDS-PAGE electrophoresis showed that when the polysaccharide addition was over 1.0%, the actin (AC) and myosin heavy chain (MHC) bands became significantly lighter, with dark bands accumulating at the top of the stacking gel. SEM observations of the microstructure of surimi gels revealed that as the polysaccharide added, the pore size within the surimi gel network gradually reduced. When the polysaccharide addition reached 1.0%, the surimi gel network structure was the most compact, with the fractal dimension (D_f) reaching a maximum value of 2.8657. Further addition of the polysaccharide leaded to phase separation in the gel system, and extensive self-aggregation of polysaccharides disrupted the surimi protein gel network, resulting in a decrease of D_f.【Conclusion】Wheat bran polysaccharide with the concentration of 1.0% to the surimi could be used as a filler as well we water retention agent in the three-dimensional network structure of surimi protein, and induce the formation of a more uniform and dense gel matrix, which could effectively improve the gel quality of surimi.

【Objective】The aim of this study was to provide the theoretical and technical support for the innovation of green, high-yield, high-quality and high-efficient unmanned cultivation technology system of wheat. 【Method】 According to the situation of accelerating land transfer and large-scale operation, decreasing labor force engaged in agricultural production, and more efficient and comfortable farming methods, the integrated unmanned cultivation technology of wheat was put forward through the integration study of “agronomy-machinery-intelligence”, that is, using new technology, new product and new equipment to simplify and integrate the whole process of wheat production, and complete wheat production with the least number of operations, the least number of machines and unmanned operations. On the basis of exploratory experimental research, the integrated unmanned cultivation technology of wheat (IU) and conventional mechanized high-yield cultivation techniques of wheat in experimental area (CK) were set up as treatments in Dazhong Farm of Yancheng, Jiangsu Province in 2019-2020, 2020-2021 and 2021-2022, to study the traits and differences of wheat yield formation among different technology treatments, analyze the high-yield traits of IU, and put forward the technical approaches of IU. 【Result】 The IU increased wheat yield by 3.0%-5.9% compared with CK, and significant differences were observed between treatments of some varieties or some growing seasons. In terms of yield components, the spike number was IU>CK (significant differences were observed between treatments of some varieties or some growing seasons), the grains per spike were IU>CK (P>0.05), the total grains were IU>CK (P<0.05), and the 1000-kernels weight was IU<CK (P>0.05), indicating that the IU increased wheat yield by stabilizing the grains per spike and 1000-kernels weight, and increasing the spike number. In the production of photosynthetic matter, the culm number, leaf area index, dry matter accumulation at the main growth stages, the leaf area duration and crop growth rate in the main growth periods, and the culm fertility and grain leaf ratio were all expressed as IU>CK (significant differences were observed between treatments of some varieties or some growing seasons), which laid a material foundation for the yield increase of the IU. This paper not only summarized the technical approaches and basic technologies of IU but also discussed the development of IU from the aspects of integrated cultivation, unmanned cultivation, “agronomy-machinery-intelligence” fusion degree, key agronomy technology and comprehensive evaluation. 【Conclusion】 The yield under IU was equivalent or significantly increased to that under CK. And the high-yield cultivation of wheat was realized with less agricultural machinery and labor and unmanned operation, which was an effective way for the development of agricultural modernization production. In the future, multi-faceted collaborative innovation and investment should be strengthened to accelerate the application and large-scale promotion of this technology.

【Objective】This study aimed to examine changes in seed vitality and physiological mechanisms under natural aging conditions in winter wheat, to analyze how different storage durations affect seed germination vitality, root growth, and antioxidant capacity, and to identify key physiological indicators of seed aging, so as to provide a basis for breeding winter wheat varieties with improved storability. 【Method】 The newly bred winter wheat cultivars, including Annong1589 (AN1589), Annong1687 (AN1687), and Annong179 (AN179), were used as experimental materials. The seeds were stored at room temperature to simulate natural aging condition. Samples were taken from seeds stored for 6 months, 18 months, and 30 months to measure seed vigor indices, such as germination rate, germination potential, germination index, vigor index, field emergence rate, and seedling root characteristics. Additionally, those key physiological indicators during the seed and seedling stages were analyzed, including electrical conductivity (Con), malondialdehyde (MDA) content, proline (Pro), superoxide dismutase (SOD) activity, catalase (CAT) activity, peroxidase (POD) activity, and the content of soluble sugars and soluble proteins in the seeds.【Result】Genotype had a significant effect on seed vigor and physiological indicators within the same storage period, with the interaction of cultivar and aging time having a notable impact on seed vigor. As storage time increased, the germination potential and root vigor of winter wheat seeds significantly declined. Germination index, vigor index, field emergence rate, and seedling stage indicators such as seedling height, fresh weight, and root fresh weight also showed a decreasing trend. Germination potential decreased most rapidly, with all three cultivars maintaining over 90% germination potential after 6 months of storage. After 18 months, the germination potential of AN1589, AN1687, and AN179 declined to 85.88%, 81.70%, and 88.58%, respectively. After 30 months of storage, the germination potential of all cultivars dropped below 80%, to 75.42%, 74.04%, and 79.17% of AN1589, AN1687, and AN179, respectively. This indicated a significant decline in seed vigor during natural aging. The study found that as the aging process progressed, the three cultivars continuously accumulated MDA and Pro, indicating increasing oxidative damage to the seed cell membranes. The initial MDA content in AN1589 was the lowest among the three cultivars, at 0.0427 μmol·g^-1. Moreover, AN1589 had the smallest increase in Pro content after 30 months of storage, at 22.43%. The activities of antioxidant enzymes of SOD, CAT, and POD, as well as the contents of soluble sugars and soluble proteins, decreased with increasing aging, indicating a gradual loss of antioxidant capacity and consumption of internal substances to support seed germination and seedling growth. 【Conclusion】Under natural aging conditions, the vigor of winter wheat seeds gradually decreased, closely related to the consumption of internal substances, increased oxidative damage, and weakened antioxidant capacity. The increase in MDA content and changes in antioxidant enzyme activity were important physiological indicators reflecting seed aging. AN1589 exhibited relatively stable vigor and physiological indicators under natural aging conditions, indicating strong storage tolerance.

Yangmai 24 as the test material, the incidence of wheat head blight in the field in 2020, 2021 and 2024 was investigated, and the influence of climate factors on it was analyzed. To explore the effects of growth stage node (early flowering and flowering stage), 200 g/L fluzoyl hydroxylamine SC and other agents, and application frequency (1 and 2 times) on the disease spike rate, disease index, disease prevention efficiency and disease finger control efficiency of wheat head blight, and observe the safety of different agents on wheat. The results showed that continuous rainy weather with average daily temperature higher than 15 ℃ was conducive to the occurrence of wheat head blight. In 2020, it was mild; in 2021, it was severe; and in 2024, it was pandemic. The applied agents had good safety for wheat growth,the application of 200 g/L fluzoyl hydroxylamine SC had a certain effect on the sensory properties of wheat. The comprehensive field control effect showed that 200 g/L fluzoyl hydroxylamine SC had a relatively prominent control effect on wheat head blight, in 2020, the disease spike rate, disease index, disease prevention effect, and disease finger control effects of the late application at the early flowering stage were 1.33%, 86.05%, 0.33 and 86.05%, respectively. When applied once at the early flowering stage, the disease spike rate, disease index, disease prevention effect, and disease finger control effects were 6.41%, 85.33%, 1.68 and 90.45% in 2021, and 11.04%, 85.20%, 3.22 and 89.93% in 2024, respectively. In conclusion, the overall window period of wheat head blight control in light years was longer, and the early flowering stage was the key period forwheat head blight control. Better control effect could be achieved by applying appropriate agents once during this period. In actual production, at the early flowering stage, it is recommended to apply 200 g/L fluzoyl hydroxylamine SC 900 mL/hm² to control wheat head blight

【Objective】The GRAS family constitutes a unique class of plant-specific transcription factors that play a pivotal role in plant development and stress response. To elucidate the function of GRAS family genes in wheat heat tolerance，which can provide genetic resources and theoretical foundation for wheat heat-resistant breeding.【Method】A potential heat stress-responsive transcription factor gene, TaGRAS34-5A, was identified through transcriptome analysis of TAM107 and Chinese spring wheat seedlings under high-temperature conditions. Subsequently, a bioinformatics analysis was performed on TaGRAS34-5A, and a phylogenetic tree was constructed to elucidate its molecular characteristics. The expression pattern of TaGRAS34-5A under various stresses, including high temperature, abscisic acid (ABA), ethylene (ETH), and salicylic acid (SA) treatments, were examined using real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) method. The subcellular localization of the TaGRAS34-5A protein was determined using wheat protoplast transient expression technique. Furthermore, the heat tolerance function of TaGRAS34-5A was validated using the heterologous expression system of Saccharomyces cerevisiae and the BSMV:VIGS (Barley stripe mosaic virus: Virus-Induced Gene Silencing) silencing technique. potential interacting proteins of TaGRAS34-5A were screened using yeast two-hybrid technology, and the heat tolerance function was verified, providing preliminary insights into its heat tolerance mechanism.【Result】TaGRAS34-5A, equipped with a characteristic GRAS domain and belongs to the GRAS transcription factor family, is localized to both the cell nucleus and cytoplasm. Bioinformatics analysis indicates that the TaGRAS34-5A promoter contains a large number of hormone response elements and light response elements, and it is most closely related to TaSCL14, OsGRAS23, and AtSCL14 in terms of phylogenetic relationships, suggesting its potential function in responding to oxidative stress. Its expression is upregulated under high-temperature, ethylene (ETH), abscisic acid (ABA), and salicylic acid (SA) treatments, peaking at 4, 6, 0.5, and 12 hours post-treatment, respectively, with the most significant induction observed under heat stress and SA. Functional assays in yeast demonstrated that heterologous expression of TaGRAS34-5A enhances the heat tolerance of the yeast. The results of BSMV:VIGS transient silencing experiment showed that after the 42 ℃ high-temperature treatment, TaGRAS34-5A silenced plants exhibited decreased chlorophyll content, reduced POD enzyme activity, increased cellular peroxidation, and decreased heat tolerance compared to the control. Preliminary studies on the heat tolerance mechanism suggest that TaGRAS34-5A exhibits strong transcriptional self-activation activity.it may modulate wheat heat tolerance by interacting with proteins such as the bZIP family transcription factor HBP-1b and the E3 ubiquitin ligase hel2, thereby regulating cellular redox homeostasis and detoxification processes, positively influencing the heat tolerance of wheat.【Conclusion】TaGRAS34-5A is induced by heat, ABA, ETH, and SA, and its encoded protein is located in the nucleus and cytoplasm. It exhibits transcriptional activation activity. Heterologous overexpression of TaGRAS34-5A enhances the heat tolerance of Saccharomyces cerevisiae. Silencing TaGRAS34-5A in wheat plants increases cellular peroxidation, decreases chlorophyll content, and reduces heat tolerance. TaGRAS34-5A may regulate the heat tolerance of wheat by modulating cellular redox state and detoxification processes.

In order to clarify the effects of adjusting sowing date on the yield and quality of spring wheat in Ningxia, 20 main spring wheat varieties (lines) in Ningxia irrigation areas were used as the experimental materials. A two-factor split-plot experiment was conducted with sowing date as the main plot and different wheat varieties as the subplot. The differences in wheat yield and quality under different sowing dates were compared and analyzed. The results showed that compared with the normal sowing date, the growth period of the tested varieties was shortened by 8-14 days after the sowing date was delayed by 20 days. The yield, effective panicle number, and thousand-grain weight were significantly reduced by 18.8%, 7.22%, and 3.54% respectively, while the number of grains per panicle did not change significantly; the hardness, wet gluten content, sedimentation value, stability time, and maximum tensile resistance increased significantly by 9.21%, 3.66%, 14.13%, 55.92%, and 160.56%, respectively, while the content of protein decreased significantly by 19.30%. There were no significant changes in bulk density and water absorption. The analysis using the membership function method revealed that under late sowing conditions, the varieties with better comprehensive performance in terms of yield and quality were 'Ningchun 52', 'Ningchun 61', 'Yongliang 15', 'Ningchun 54', 'Ningchun 53', 'Ningchun 48', and 'Ningchun 56'. Among these, 'Ningchun 52' and 'Ningchun 61' had the highest comprehensive evaluation values, which was 0.8. Delayed sowing date had a significant impact on the yield and quality of spring wheat.