To screen the suitable sowing date and planting density combination for ‘Xinmai 58’, a two factor split field experiment was conducted in the National Agricultural High Tech Zone Demonstration Park in Dancheng County, Henan Province from 2023 to 2024, with the sowing dates (October 17, October 25, November 1, November 8) being the main area and the sowing amounts (150 kg/hm², 187.5 kg/hm², 225 kg/hm²) being the sub area, to explore the effects of different sowing dates and planting densities on the growth stage, population at different stages, dry matter accumulation, plant height, yield, and yield of ‘Xinmai 58’. The results indicate that: as the sowing periodis postponed, all growth stages are delayed, but the growth period is shortened, and the impact of planting density on the growth period is relatively small. The impact of different sowing periods on the population at different stages is relatively small, and sowing amountis the main factor affecting the population. The effects of different treatments on dry matter weight and yield are inconsistent. Late sowing affects dry matter accumulation, but increasing sowing amount can compensate for the loss of yield. The main factor affecting the number of spikes is sowing amount, and sowing date has a significant impact on the number of grains per ear, thousand grain weight, and plant height. Different treatments and their interactions result in significant differences in yield. The suitable sowing period for ‘Xinmai 58’ is from October 17th to 25th, with a suitable sowing amount of 187.5 kg/hm². If the sowing period is too late,, increasing the sowing amount appropriately can improve the yield level.

To investigate the effects of combinations of fertilizer, microbial inoculant and immune inducer on soil carbon and nitrogen contents and wheat yield, Shengmai 711 was used as the test wheat variety. In terms of experimental design, 7 treatments were established, including organic compound fertilizer (D), Trichoderma compound microbial inoculant (J), immune inducer (Z), combined application of organic compound fertilizer and immune inducer (DZ), combined application of organic compound fertilizer and Trichoderma compound microbial inoculant (DJ), combined application of Trichoderma compound microbial inoculant and immune inducer (JZ), and combined application of organic compound fertilizer, Trichoderma compound microbial inoculant and immune inducer (DJZ), with the treatment of applying the compound fertilizer commonly used by local farmers, no microbial inoculant applied and foliar application of clear water set as the control (CK). In terms of experimental methods, soil samples from 0-10, 10-20, 20-30, 30-40 and 40-50 cm layers were collected at the jointing stage, flowering stage and maturity stage of wheat by the “S”-shaped sampling method, and the contents of soil total nitrogen, organic carbon, urease activity, ammonium nitrogen (NH₄-N), nitrate nitrogen (NO₃-N) as well as wheat yield were determined. The results showed that under the DJZ treatment, the total nitrogen content in the 0-30 cm soil layer (from shallow to deep) at the maturity stage increased by 51.42%, 46.85% and 93.41% respectively compared with the CK. Soil organic carbon content in all soil layers was higher under DJZ treatment at the jointing, flowering and harvest stages. The soil urease activity was relatively high at the flowering stage, and the DJZ treatment had higher urease activity in the 0-50 cm soil layer than other treatments. Overall, the contents of NH₄-N and NO₃-N in each soil layer at the maturity stage and flowering stage were lower than those at the jointing stage. Under the DJZ treatment, the NH₄-N content in all soil layers at the flowering stage and harvest stage was higher than that in the CK. For NO₃-N content, it was higher in all soil layers under the DJZ treatment than in the CK treatment at the jointing stage and harvest stage, except for the 20–30 cm soil layer at the harvest stage. The wheat yield under all treatments was higher than that of the CK treatment; among these treatments, the DJZ treatment resulted in the highest number of spikes per unit area (283 300 spikes/hm⊃2;) and the highest yield (5 698.14 kg/hm⊃2;). In conclusion, the combined application of organic compound fertilizer, Trichoderma compound microbial inoculant and immune inducer improved the physical and chemical properties of soil, increased the contents of soil organic carbon and total nitrogen in the 0-50 cm soil layer at the jointing, flowering and harvest stages, elevated NH₄-N content in the 0-50 cm soil layer at the harvest stage, and enhanced urease activity in the 0-50 cm soil layer at the flowering and harvest stages, thus contributing to increase wheat yield.

This paper systematically reviewed and analyzed the characteristics of wheat wide-precision sowing technology and its impacts on wheat growth and yield. As a high yield sowing technology centered on expanding sowing width and increasing row spacing (the planting row spacing was increased to 22–25 cm and the sowing width was expanded to 8–10 cm), it adopted the method of dispersed grain sowing (with a seeding rate of 112.5 kg/hm⊃2;). This technology enabled precise and uniform sowing of wheat seeds as well as one-time soil compaction operation, while reducing production costs. The specific effects of this technology on wheat growth and yield were as follows: under this technical mode, wheat seedlings emerged evenly with consistent growth, which improved seed survival rate and seedling emergence rate; it effectively optimized the ventilation and light transmission conditions among wheat plants, and the above-ground and below-ground indexes of wheat, such as tiller number, heading rate, root length density and root weight density, were all higher than those under conventional sowing; it fully utilized light resources, improved the utilization efficiency of water and fertilizers, and realized the efficient utilization of environmental resources; it significantly increased yield-related indexes including grain weight per spike and 1 000-grain weight of wheat, thus achieving the goal of high yield. This paper aims to provide a reference for the further popularization and application of wide-precision sowing technology for wheat by integrating existing research findings.

Liangxing 77 was used as the test wheat cultivar, and the field efficacy trials were conducted in Yinma Town (A), Fuchun Town (B) and Yanshi Town (C) of Juancheng County, Shandong Province. 4 treatments were set up, including 40% carbendazim SC at 1 875 g/hm⊃2; (T1), 430 g/L tebuconazole SC at 450 g/hm⊃2; (T2), 25% phenamacril SC at 2 400 g/hm⊃2; (T3), and water control (CK), with the first and second pesticide applications carried out on April 25 and 29, 2022, respectively, followed by investigations of the incidence of diseased spikes, control efficacy against diseased spikes, disease index, and control efficacy based on disease index for each treatment; strains were isolated, collected and identified from sites A, B and C in 2023 and 2024, and after activation, the tested strains were inoculated onto drug-free potato dextrose agar (PDA) medium (control plates) and drug-containing PDA medium (treatment plates) to determine the resistance frequency for evaluating the resistance level of Fusarium graminearum; the results showed that across the three test sites, the incidence of diseased spikes and disease index of all chemical treatments followed the order of T1 > T2 > T3, whereas the control efficacy against diseased spikes and that based on disease index showed the opposite order of T3 > T2 > T1; the resistance frequency of the pathogen causing wheat Fusarium head blight to carbendazim exceeded 50% in sites A and C (except for site C in 2024), that to tebuconazole was relatively high in site A, while the overall resistance frequency to phenamacril was low; correlation analysis indicated a significant negative correlation between the pathogen resistance level and the field control efficacy of the fungicides, and this study provides a theoretical basis for the sustainable control of wheat Fusarium head blight, recommending the rotation of fungicides with high efficacy and low resistance risk as well as the strengthening of resistance monitoring and rational application of fungicides with emerging resistance trends.

【Objective】This study aimed to develop novel glyphosate-resistant wheat germplasm using EMS mutagenesis to mitigate weed infestation in wheat fields. Resistant mutant plants were selected through field screening, and the mutation profiles of the 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene as well as optimal application conditions were characterized, offering a practical approach for breeding glyphosate-resistant wheat varieties.【Method】A mutant population was generated by treating newly germinated seeds of Zhenmai 9 with EMS mutagenesis. Resistant mutants were isolated through multiple rounds of glyphosate screening in the field across M₂ and M₃ generations. Promising lines, including GR1, GR19, and GR23, were identified via pedigree selection, combined with yield and resistance phenotype screening. Mutation sites in the EPSPS gene were detected by PCR amplification and sequencing, while expression levels of TaEPSPS-4A, TaEPSPS-7A, and TaEPSPS-7D were analyzed using RT-qPCR. Field trials involving different glyphosate doses and application growth stages were conducted to systematically evaluate herbicide efficacy and determine the appropriate dosage and timing for safe application.【Result】The resistance mutant frequency in the M₂ population was 6.53×10^-6. In the M₃ generation, 43 mutant plants exhibiting tolerance to 4× the recommended glyphosate dose were successfully obtained. Sequencing analysis revealed that resistant lines GR1 and GR19 harbored 5 and 3 mutation sites in TaEPSPS-7D, respectively, whereas GR23 carried 5 mutation sites in TaEPSPS-4A. Expression analysis indicated that glyphosate treatment significantly downregulated most homoeolog genes in the three mutation lines, irrespective of whether those genes carried resistance mutations. Field trials demonstrated 100% weed control efficacy across all glyphosate treatments, significantly superior to isoproturon. As the glyphosate doses increased, wheat seedling height and fresh weight showed a decreasing trend, but most differences with the untreated control were not significant, indicating no substantial adverse effects on growth. Yield analysis revealed that treatment with 1× and 2× doses did not cause significant yield reduction, whereas 4× and 8× doses led to significant reductions of 3.04% and 4.63%, respectively. Growth stage-specific trials further indicated that spraying a 2× dose of glyphosate from seedling to jointing stages had no significant impact on plant growth, but application at the booting stage significantly reduced plant height, fresh weight, and grain yield, resulting in a 6.48% yield loss.【Conclusion】The combination of EMS mutagenesis and field screening successfully generated new glyphosate-resistant wheat germplasm capable of withstanding 4× the recommended glyphosate dose. Multiple point mutations in the non-active center of the EPSPS enzyme confered enhanced glyphosate resistance without compromising yield. For practical application of such resistant varieties, the optimal weed control window is during wheat green-up (early March), using 41% glyphosate isopropylamine salt at 840-1 680 g ae·hm^-2, diluted in 450 L·hm^-2 of water, applied as foliar spray to weeds under rain-free conditions.

Combined with the planting practice of late-sown wheat in Southern Jiangsu Province, the causes of late sowing and its impacts on wheat growth and development were analyzed, and a targeted cultivation technical system was integrated for demonstration application. The main causes of wheat late sowing in the study area were the stubble conflict in rice-wheat rotation (the long growth period of high quality rice varieties) and climate change (precipitation gradually decreases in autumn and winter). The delayed sowing date led to a significant decline in the growth process and population quality of wheat, and ultimately caused wheat yield reduction. Based on this, this paper constructs a comprehensive cultivation technology system of “late-sowing tolerant variety + agronomic strong compensation + prevention and control of forward shift”. Specifically, the technical measures included the following aspects: selecting spring wheat varieties (e.g., Yangmai 25、Yangmai 33 and Zhenmai 10); dynamically adjusting the seeding rate (increasing by 4.0-7.5 kg/hm⊃2; for each day of delayed sowing); applying sufficient base fertilizer (containing 60%-70% of the total nitrogen fertilizer, all phosphorus fertilizer and potassium fertilizer); early applying green-up fertilizer (in early February, with a nitrogen application rate of 30-50 kg/hm⊃2;); lightly applying jointing fertilizer and booting fertilizer (in late February or early March, using 0.3% potassium dihydrogen phosphate); timely spraying plant growth regulators such as paclobutrazol to prevent lodging; applying herbicides including 70% flucetosulfuron and 50% isoproturon to control weeds; applying 40% prothioconazole·tebuconazole suspension concentrate, 25% thiamethoxam·lambda-cyhalothrin microemulsion and amino acid foliar fertilizer to prevent and control diseases and insect pests like wheat scab and aphids; harvesting at the late dough stage to early maturity stage, and timely drying and storing after harvesting. The demonstration results in Lishui District Hefeng Town of Nanjing in 2024 showed that actual yield of wheat in the experimental plots (adopting late-sowing cultivation techniques) reached about 5 600 kg/hm⊃2;, in the control plots (using conventional techniques) was 5 100 kg/hm⊃2;. Practice showed that the late-sown wheat cultivation technology integrated in this paper could provide a reference for the efficient and green production of late-sown wheat.

Fusarium head blight, as one of the major diseases of wheat, significantly impacts both yield and quality. This study systematically reviewed the pathogen characteristics, occurrence patterns, disease development conditions, and associated hazards of Fusarium head blight, while proposed scientifically prevention and control strategies. The primary pathogen of Fusarium head blight was identified as fungi of the Fusarium genus, which have a wide range of hosts and strong pathogenic and spreading abilities. Wheat was most susceptible during the flowering to heading stages, and disease epidemics were strongly influenced by temperature and humidity. Persistent rainy weather and temperatures of 20-25 ℃ were found to favor disease development. The damage caused by head blight manifested as seedling rot, crown rot, stem rot, and head blight. Among these, head blight directly reduced yield and quality, and infected grains could produce deoxynivalenol, posing a threat to human and animal health. Based on the disease occurrence patterns and characteristics, control measures were proposed. These included the management of soil and crop residues to eliminate or reduce pathogens, the selection of disease-resistant or tolerant varieties such as Yangmai 28, the implementation of scientific field management practices (e.g., rational planting density and water-fertilizer regulation), precise spraying of beneficial bacterial agents such as Bacillus during the flowering period, and the judicious application of agents such as 50% carbendazim during the wheat heading and flowering stages. This study provided a reference for sustainable wheat production.

To clarify the control efficacy of 10 fungicides including 25% prothioconazole·pyraclostrobin suspension concentrate (SC) against wheat Fusarium head blight (FHB), using Yangmai 25 as the test variety and following a randomized block design, a total of 11 treatments were set up, which included clear water control, 25% prothioconazole·pyraclostrobin SC at 900 mL/hm⊃2; (T1), 30% prothioconazole·azoxystrobin SC at 750 mL/hm⊃2; (T2) and 30% prothioconazole·trifloxystrobin SC at 750 mL/hm⊃2; (T3), 40% prothioconazole·tebuconazole SC (Jiuyi) at 600 mL/hm⊃2; (T4), 40% prothioconazole·tebuconazole SC (Zhongnan) at 750 mL/hm⊃2; (T5), 30% prothioconazole OD at 675 mL/hm⊃2; (T6), 75% prothioconazole WG at 255 g/hm⊃2; (T7), 480 g/L fenoxanil·tebuconazole SC at 900 mL/hm⊃2; (T8), 200 g/L fluxapyroxad SC at 975 mL/hm⊃2; (T9), and 275 g/L fluxapyroxad·propiconazole SC at 1 200 mL/hm⊃2; (T10), and fungicide applications were performed twice, respectively at the early flowering stage of wheat (the first application) and 7 days after the initial spray (the second application), followed by investigations on the safety of each treatment to wheat and the field control efficacy against FHB. The results showed that all treatments were safe for wheat growth, the diseased spike rates of treatments T1 to T10 ranged from 2.35% to 7.85%, the diseased spike control efficacy was ranked in a descending order as follows: T9 > T6 > T5 > T10 > T4 > T7 > T1 > T2 > T3 > T8, and the control efficacy against disease index varied from 89.88% to 97.22%, among which treatment T9 achieved the highest efficacy while treatment T8 had the lowest one. In conclusion, all the tested fungicides are suitable for the field control of wheat FHB, but rotational application is recommended, so as to ensure the control efficacy and delay the development of resistance in Fusarium pathogens.

Microplastic accumulation after film mulching affects nutrients cycling in the soil-crop system.  Bulk soil (BS) and rhizosphere soil (RS) have two different community compositions which lead to their different microbial nutrient acquisition abilities. Microplastics influence the rhizosphere effect. However, the mechanism by which microplastic accumulation affects the net photosynthetic rate (NPR) through rhizospheric microbial communities remains unknown. This study aimed to identify the mechanisms underlying the effects of polyethylene (PE) and polyvinyl chloride (PVC) microplastics at 0, 1, and 5% (w/w) on the NPR in the wheat-soil ecosystem using a pot experiment. Superoxide dismutase (SOD) activity was reduced by 15.35–36.7%, and that of peroxidase (POD) was increased by 32.47–61.93%, causing reductions in NPR (17.94–23.81%) in the PE5% and PVC (1 and 5%) (w/w) treatments compared with the control. The Chao1, Shannon, and Simpson indices of the bacterial and fungal diversities were lower in BS than in RS at PE1% and PVC5% (w/w), respectively. The bacterial and fungal network complexities were reduced and increased, respectively, owing to alterations in the bacterial and fungal community compositions and structures for wheat growth. The Mantel test showed that the bacterial and fungal diversity indices in BS had positive correlations with Olsen-P and phosphatase; however, those in RS were positively correlated with NO₃^- and β-1,4-glucosidase. The structural equation model indicated that wheat enzymatic and soil hydrolytic activities negatively affected NPR. Wheat has a profound antioxidant defense strategy for PE and PVC microplastic stress, which produces a synergistic effect of POD by protecting organelles and reducing tissue damage to preserve the NPR.

Structural variation is an important source of genetic variation in wheat and have been important in the evolution of the wheat’s genome. Few studies have examined the relationship between structural variations and agronomy and drought tolerance. The present study identified structural chromosome variations (SCVs) in a doubled haploid (DH) population and backcross introgression lines (BC₅F₃) derived from Jinmai 47 and Jinmai 84 using fluorescence in situ hybridization.  There are one simple translocation, 10 present/absent variations (PAVs), and one copy number variation (CNV) between Jinmai 47 and Jinmai 84, which distributed in 10 chromosomes.  Eight SCVs were associated with 15 agronomic traits. A PAV recombination occurred on chromosome 2A, which was associated with grain number per spike (GNS). The 1BL/1RS translocation and PAV.2D were associated with significant reductions in plant height, deriving from the effects on LI2-LI4 and UI, LI2-LI4, respectively.  PAV.2D was also contributed to an increase of 3.13% for GNS, 1BL/1RS significantly increased spikelet number, grain length (GL), and grain thickness (GT). The effect of PAV.4A.1 on GL, PAV.6A on spike length (SL) and thousand-grain weight (TGW), PAV.6B on SL, GT and TGW were identified and verified. PAVs on chromosomes 2A, 6A, 1D, 2D, and a CNV on chromosome 4B were associated with the drought tolerance coefficients.  Additive and interaction effects among SCVs were observed. Many previously cloned key genes and yield-related QTL were found in polymorphic regions of PAV.2B, PAV.2D, and CNV.4B.  Altogether, this study confirmed the genetic effect of SCVs on agronomy and drought tolerance, and identification of these SCVs will facilitate genetic improvement of wheat through marker-assisted selection.

The development of wheat cultivars with improved nitrogen (N), phosphorus (P) and potassium (K) use efficiency is desirable for sustainable agriculture.  Genetic dissection and identification of causative genes for nutrient use efficiency is the ideal strategy to achieve this goal.  We conducted an extensive genome-wide association study (GWAS) employing a panel of 431 genotyped wheat cultivars, revealing 1659 significant single-nucleotide polymorphisms (SNPs) (LOD>5) via genotyping-by-sequencing.  Our investigation uncovered 534 quantitative trait loci (QTLs) associated with 12 nutrient use efficiency traits across five distinct environments, of which 14 QTLs were consistently identified in at least three environments.  Through the integration of meta-QTL analysis, it is noteworthy that QTL80 (72.12-74.24 Mb, chr2A), QTL387 (32.88-33.56 Mb, chr6A), and QTL500 (535.53-540.80 Mb, chr7B) manifest a distinct co-localization with MQTL-2A-2, MQTL-6A-1, and MQTL-7B-2, respectively.  This convergence underscores their substantive relevance across a spectrum of diverse environmental conditions.  Within these regions, pivotal candidate genes, such as the bZIP transcription factor family gene and potassium transporter gene, associated with nutrient use efficiency were discerned.  Furthermore, a novel locus, QTL234, emerged, housing key candidate genes like dof zinc finger protein, Ankyrin repeat family protein, and cytochrome P450.  To validate the SNP located within QTL234 associated with nitrogen harvest index (NHI), we developed a dCAPS marker for AX-109095537.  These findings underscore the efficacy of high-resolution SNP-based GWAS in swiftly identifying potential key candidate genes.  Additionally, they solidify the groundwork for large-scale QTL fine mapping, candidate gene validation, and the development of functional markers crucial for advancing nutrient use efficiency breeding in wheat.

Fusarium head blight (FHB), mainly caused by fungus Fusarium graminearum (F. graminearum), is a devastating wheat disease worldwide, leading to reduced yield production and compromised grain quality due to contamination by mycotoxins, such as deoxynivalenol (DON). Manipulating the specific gene expression in microorganisms through RNA interference (RNAi) presents an opportunity for new-generation double-stranded RNA (dsRNA)-based formulations to combat a large number of plant diseases. Here, we applied both spray-induced gene silencing (SIGS) and host-induced gene silencing (HIGS) to target five virulence-related and DON-synthesized genes in F. graminearum, including protein kinase gene Gpmk1, zinc finger protein gene FgChy1, transcription factor FgSR, DON synthesis gene TRI5 and the cell-end marker protein gene FgTeaA, aiming to effectively control FHB in wheat. Direct spraying of individual or combined siRNAs (small interfering RNA) from the fungus showed reduced expression of target genes and suppressed pathogenic symptoms during F. graminearum infection in wheat leaves, with the combination of all five siRNAs demonstrating superior resistance. Furthermore, we generated transgenic wheat lines expressing chimeric RNAi cassettes targeting these five genes, and two independent lines exhibited strong resistance to FHB and Fusarium crown rot, and the reduced DON accumulation. Notably, the HIGS transgenic lines did not adversely impact plant growth and yield traits. Collectively, our findings support that SIGS and HIGS represent effective strategies targeting key pathogenic genes for bolstering disease resistance in crops.

【Objective】Thousand-grain weight (TGW), a key determinant of final wheat yield, is of great importance for genetic dissection. Precise identification of stable loci and key candidate genes controlling TGW provides theoretical foundations and genetic resources for marker-assisted breeding of high-TGW and high-yield wheat varieties. 【Method】A total of 291 wheat accessions from diverse origins were genotyped using a 100K SNP array. TGW phenotypic data collected over two consecutive years and their best linear unbiased predictions (BLUPs) were analyzed using a genome-wide association study (GWAS) based on a mixed linear model (MLM) incorporating both population structure (P) and kinship (K). Significant loci were further subjected to haplotype analysis. 【Result】TGW showed broad variation across years and BLUP values (mean: 38.24-38.82 g; coefficient of variation: 17.62%-19.93%). The correlation between years was 0.88 (P<0.01), and correlations with BLUP values reached 0.97 (P<0.01). Phenotypic data displayed normal distributions under different environments, meeting the basic requirements for GWAS. A total of 19 SNPs significantly associated with TGW were detected on chromosomes 3B, 5A, and 7A, explaining 6.85%-9.68% of the phenotypic variation; 16 of them were repeatedly detected across multiple environments, indicating stability. Haplotype analysis at locus 7A_145980808 revealed four haplotypes (Hap1-Hap4), of which Hap4 was associated with high TGW (P<0.01) and Hap2 with low TGW (P<0.01). The frequencies of Hap1-Hap4 were 72.36%, 14.55%, 8.73%, and 4.36%, respectively. Domestic accessions were enriched for Hap3 (95.83%) and Hap4 (83.33%), with Hap4 predominantly distributed in the Northwestern winter wheat region, especially in germplasm from Gansu. Candidate gene mining within 3.6 Mb regions flanking significant loci on chromosomes 3B, 5A, and 7A identified 95 genes, among which four were highlighted based on gene annotation and expression profiles. 【Conclusion】GWAS identified 16 stable SNP loci significantly associated with TGW, four distinct haplotypes, and four key candidate genes. These genes are mainly involved in carbohydrate synthesis and transport, cell wall polysaccharide assembly, protein homeostasis, and transcriptional regulation of starch biosynthesis, providing valuable targets for molecular breeding of high-yield wheat.

【Objective】Zn (Zinc) deficiency triggers ‘hidden hunger’. Enhancing Zn concentration in wheat grains and Zn fertilizer use efficiency through biofortification can effectively increase dietary Zn intake, thereby improving human Zn nutritional status. 【Method】The study subjects were two distinctive colored-grain wheat varieties: ‘Taihei 5’ (purple-grained) and ‘Tailan 8’ (blue-grained). A two-year field experiment was conducted from 2022-2024 in Taigu District, Jinzhong City, Shanxi Province. Foliar Zn application was performed at 3-5 days after the flowering of colored-grain wheat (Over 50% of spikes in the wheat field had lemma and palea separation at middle-upper florets while anthers were dehiscing). Five Zn concentration treatments were applied: Zn0 (deionized water), Zn1 (Zn concentration: 440 mg·L^-1), Zn2 (Zn concentration: 587 mg·L^-1), Zn3 (Zn concentration: 733 mg·L^-1), Zn4 (Zn concentration: 880 mg·L^-1). Through analysis of grain yield and Zn concentrations in grains, leaves, and stems across multiple post-anthesis periods for both colored-grain wheat types, Zn concentration variation dynamics, Zn accumulation and partitioning characteristics, Zn utilization efficiency, grain Zn biofortification index and grain Zn harvest indices were quantitatively analyzed to evaluate their Zn biofortification efficacy. 【Result】Foliar Zn application significantly increased Zn concentrations in all organs and grain yield of colored-grain wheat, The Zn3 treatment produced the highest grain Zn concentration (21.79-67.90 mg·kg^-1) and peak grain yield (4 937.36-5 097.27 kg·hm^-2). Grain Zn accumulation reached its optimum (251.30-301.54 g·hm^-2) under the Zn3 treatment, while Zn concentrations and accumulation in leaves and stems increased linearly with rising application concentrations. With increasing Zn application concentrations, the grain Zn accumulation proportion showed a declining trend (10%-18%), while the leaf Zn accumulation proportion rose to 66%, and stem Zn accumulation remained at 23%-30%. Furthermore efficient synergy in Zn utilization efficiency across all organs of colored-grain wheat was achieved under Zn3 treatment (5.68%-7.70%). With increasing Zn application concentrations, the grain Zn biofortification index and Zn harvest index declined. Compared with Zn1, other Zn treatments reduced the grain Zn biofortification index by 12.50%-47.02%, while relative to the control (Zn0), all Zn treatments decreased the Zn harvest index by 23.66%-60.44%. ‘Taihei 5’ outperformed ‘Tailan 8’ in grain Zn concentration, accumulation, utilization efficiency, and biofortification performance. Possibly influenced by precipitation, both types of colored-grain wheat performed better in the second growing season 【Conclusion】Post-anthesis foliar Zn application effectively regulated Zn accumulation and partitioning in colored-grain wheat. The combination of purple-grained wheat varieties and foliar Zn application at 733 mg·L^-1 achieved the optimal balance between grain Zn concentration and Zn utilization efficiency in colored-grain wheat systems.

【Background】Improving water use efficiency is beneficial for the sustainable production of wheat. Both ridge and furrow planting and soil moisture-based supplemental irrigation techniques can significantly enhance crop water use efficiency. However, whether the combination of these two approaches can achieve effective water-saving outcomes and further improve the water use efficiency of wheat remains unclear. 【Objective】This study aimed to explore the effects of ridge-furrow planting combined with soil moisture measurement and supplementary irrigation on the population, yield and water use efficiency of wheat. 【Method】In this study, a two-year field experiment was conducted. The winter wheat variety Xinong 20 was selected, and three planting methods of furrow sowing (P1), ridge sowing (P2) and flat planting (P3) were used. Three irrigation treatments were set up, including supplementary irrigation of soil moisture content in the 0-40 cm soil layer to field water holding capacity (S40), supplementary irrigation of soil moisture content in the 0-60 cm soil layer to field water holding capacity (S60), and traditional flood irrigation as the control irrigation (SCK). By measuring the soil moisture content, the dynamics of tillers of winter wheat, dry matter accumulation, yield and its constituent factors, and calculating the total water consumption of farmland, precipitation use efficiency, irrigation water use efficiency, total water consumption use efficiency, border row index and economic benefits, the effects of ridge-furrow planting and soil moisture measurement-based supplementary irrigation techniques on the growth and development, yield, water use efficiency and economic benefits of winter wheat were explored. 【Result】The furrow sowing combined with soil moisture measurement and supplementary irrigation at 60 cm depth (P1S60) maintained similar soil water content, tillers number, and dry matter accumulation as the flat planting with traditional flood irrigation (P3SCK). By leveraging the marginal effect, furrow sowing increased spike number and grains per spike of border-row winter wheat. For ensuring stable wheat yield, P1S60 saved 34.5% of irrigation water and reduced total farmland water consumption by 10.8%. It also boosted irrigation water use efficiency (IWUE) by 79.5% and water use efficiency (WUE) by 14.7% (two-year average). Compared with P3SCK, P1S60 raised total income by 3.2%, indicating high economic viability. 【Conclusion】Considering the utilization efficiency of water resources, yield and income potential, P1S60 was a planting method with high potential in Guanzhong irrigation area.

The aim is to screen out high-yield, stable and tolerant to late sowing wheat varieties. By comparing the differences in agronomic traits and yield of 17 wheat varieties, the study aims to provide a basis and theoretical support for the selection of varieties for high yield and stable yield of wheat in late sowing areas. Using CJ580 and other 16 wheat varieties as test materials, a single-factor random block design was employed to measure the three key yield components at maturity, and convert the grain yield according to 13% moisture content. The number of tillers per plant and the earing rate were measured at various growth stages. At maturity, 20 representative plants were randomly selected to measure plant height and ear length. The main diseases that are likely to occur under late-sowing conditions were monitored, and their severity was assessed visually during the peak disease period. Data were analyzed using SPSS and the LSD method. The results showed that there were significant differences in the tiller dynamics of different varieties at various growth stages: YF19 had the highest number of tillers during the winter survival stage, YF19, ZM18, and YM23 tied for first place during the green-up stage, and YM1 reached its peak (1008×10⁴/hm²) during the jointing stage. CJ580, YM43, and YM36 performed optimally during the earing and milk stages, with the tiller-to-earing rate ranging from 44.64% to 49.79%. In terms of plant height and ear length, YM1, YM34, and YM46 stood out (plant height> 66 cm, ear length> 8 cm), while ZM23 and YM30 were significantly lower. Disease monitoring showed that all varieties had not experienced Fusarium head blight, rust, or lodging, with only a few showing mild powdery mildew. The study found that late sowing generally led to reduced yields, shortened growth periods to 200 days, and a general decrease in plant height. Outstanding varieties regulate the three key factors of yield through a differentiated compensation mechanism. These factors include high ear numbers (YM43), balanced agronomic traits (YM1), ear-to-grain number advantage (YM34), and outstanding thousand-grain weight (YM46), all achieving stable yields above 6500 kg/hm². This study provides practical guidance for variety screening under extreme climates, but the conclusions need to be further validated through multi-year and multi-regional trials. In summary, ‘YANGMAI 43’, ‘YANMAI 1’, ‘YANGMAI 34’, and ‘YANGMAI 46’ are suitable for initial promotion in Hanjiang District, Yangzhou under extreme climates and late sowing conditions as late-sowing-resistant and stable varieties.

To investigate the effects of different sowing dates and seeding rates on the growth and yield of winter wheat in the Chengdu Plain, a two-factor split-zone experiment was adopted using the wheat variety 'Chuanmai 1247'. The main plots were designed with five sowing dates (S₁: November 1^st, traditional sowing date; S₂: November 8^th; S₃: November 15^th; S₄: November 22^nd; S₅: November 29^th), and the subplots were set with two seeding rates (R₁: 225 kg/hm²，traditional seeding rate; R₂: 300 kg/hm²). The effects of each treatment on wheat growth period, tiller dynamics, dry matter accumulation and yield were systematically studied. The results showed that compared with traditional sowing date S₁, the entire growth period of wheat under S₂-S₅ treatments was shortened by 4-17 days, which was mainly reflected in the shortening of the reproductive growth stage. As sowing delayed, the productive tiller percentage increased by 21.87% to 39.69% compared with S₁. The number of effective spikes, dry matter accumulation at the maturity stage, and yield initially increased and subsequently decreased with sowing delayed, and all reached the maximum under S₃ treatment, which increasing by 24.46%, 2.40%, and 15.43% respectively, compared with S₁. However, the thousand-grain weight consistently decreased with sowing delayed. Higher seeding rate (R₂) led to higher tiller numbers of wheat at all growth stages but reduce the productive tiller percentage. Increasing the seeding rate under traditional sowing date (S₁) reduced grain yield. Conversely, increasing the seeding rate under delayed sowing conditions could increased grain yield. In summary, delayed sowing date shortens the reproductive stage of wheat. Delay sowing appropriately (e.g, S₂-S₃) can increase grain yield by increasing the number of effective spikes and dry matter accumulation. Increasing seeding rate under traditional sowing date is detrimental to yield formation, whereas increasing seeding rate under delayed sowing date can stabilize or enhance yield. This study provides technical support for the high-yield cultivation of wheat following rice.

To investigate the mechanism of photosynthetic physiological response of spring wheat under different moisture conditions in semi-arid rain-fed agricultural areas and to optimize field management, the present study was carried out with spring wheat ‘Dingxi No. 48’ as the research object. Seven treatments were set up with three replications for each treatment, namely: film mulching (FM), irrigated treatment 1 (GG₁), irrigated treatment 2 (GG₂), drought treatment 1 (GH₁), drought treatment 2 (GH₂), drought treatment 3 (GH₃), and rain-fed treatment (YY), to measure the light response curves of spring wheat at different periods under the seven treatments. Five models were chosen, namely, the right-angle hyperbola model, the non-right-angle hyperbola model, the leaf drift model, the exponential model and the double exponential model, to fit and compare the light response curves before and after the irrigation of spring wheat. The results were analyzed for accuracy and the optimal model was selected. The results showed that: (1) the light response curve increased rapidly when the photosynthetically active radiation was 0-400 μmol/(m²·s), and slowed down when it was 600-800 μmol/(m²·s), and then stabilized or showed a decreasing trend. The leaf area index of spring wheat decreased after filling, and the photosynthetic capacity weakened, and the magnitude of the maximum net photosynthetic rate at the maturity of each treatment was in the following order: FM, GG₁, YY, GH₃, GG₂, GH₂, and GH1. (2) Combining the MAE, RMSE and R² of the measured and fitted values of the five models shows that the leaf float model has the highest fitting accuracy. The mulching treatment had the best drought tolerance and the widest range of light adaptation when planting spring wheat in the semi-arid rain-fed agricultural region of Northwest China, and the leaf floating model among the five models best matched the actual photosynthetic characteristics of spring wheat.

The breeding process, characteristics, and key cultivation techniques of Tianyikemai No.10 were summarized, a wheat variety developed through pedigree selection using Zhongmai 895 as the female parent and Huachengmai 1688 as the male parent. It was approved by the Anhui Provincial Crop Variety Approval Committee in 2024 (Wanshenmai 2024T019). Results from two-year regional trials and one-year production trials demonstrated that this variety exhibits moderate tillering and panicle formation efficiency, favorable maturity appearance, and an average yield of 9 537.50 kg/hm². Classified as a medium-gluten wheat variety, it shows moderate resistance to Fusarium head blight and susceptibility to sheath blight. For cultivation in the regions along the Huai River and in Northern Anhui, key techniques include deep plowing and rotary tillage for fine land preparation, sowing during the optimal period from October 10 to 25 at a seeding rate of 135-150 kg/hm², and a sowing depth of 3-5 cm. Scientific fertilizer management involves precise application of reviving fertilizer, jointing fertilizer, and grain-filling fertilizer, with supplemental nitrogen and foliar fertilizers adjusted according to seedling conditions. Water management emphasizes sowing under adequate soil moisture, along with timely winter irrigation and irrigation during the jointing and booting stages. Pest, disease, and weed control prioritize prevention, including removal of pathogen sources before sowing and targeted management of rust, Fusarium head blight, aphids, and weeds during the growth period. Precise pesticide application during critical stages is recommended. Mechanical harvesting should be conducted at the late wax-ripening stage, and grains can be safely stored when moisture content drops below 13%. This paper provides a reference for the promotion and cultivation of this variety in suitable regions.

To further popularize the soil testing and formulated fertilization technology for wheat and improve fertilizer utilization efficiency, a field experiment on fertilizer use efficiency was conducted with Yangfumai 4 as the test material. The experiment was carried out in Fengle Town, Feixi County, Anhui Province, from October 2023 to June 2024, with two formulated fertilizers of different nutrient ratios selected (formulated fertilization 1 with an N:P₂O₅:K₂O ratio of 12.9:4.7:5.5; formulated fertilization 2 with an N:P₂O₅:K₂O ratio of 12.5:5.0:6.5). 5 treatments were set for each formulated fertilizer, namely blank control, nitrogen-free, phosphorus-free, potassium-free and formulated fertilization. The effects of different fertilization treatments on wheat yield, yield components, fertilizer use efficiency and economic benefits were determined. The results showed that formulated fertilization significantly promoted the effective panicle number, grain number per panicle, 1 000-grain weight and actual yield of wheat. The yields of formulated fertilization 1 and formulated fertilization 2 treatments were 6 232.5 and 6 147.6 kg/hm², respectively. The nitrogen, phosphorus and potassium use efficiencies of formulated fertilization 1 and formulated fertilization 2 treatments reached 44.7%, 25.9%, 72.0% and 49.1%, 22.1%, 66.6%, respectively. The benefit-cost ratios of the two treatments were 1.96 and 1.89, respectively. In conclusion, soil testing and formulated fertilization could increase wheat yield, fertilizer utilization efficiency and benefit-cost ratio. It is recommended that the formulated fertilizer with an N:P₂O₅:K₂O ratio of 12.9:4.7:5.5 should be applied for wheat production in the study area.

In China, Xinjiang is a relatively independent epidemic region of wheat stripe rust, caused by Puccinia striiformis f. sp. tritici, due to great population genetic divergence of Xinjiang with other inland epidemic regions.  In this region, race evolution was usually slower than inland populations.  However, many new races have recently been found, and therefore, it is necessary for more understanding of the virulence evolution of the Xinjiang population.  So, in this study, a 65 sexual progenies, derived from a Xinjiang single-urediospore isolate BGTB-1 of P. striiformis f. sp. tritici by selfing on alternate host barberry (Berberis aggregata).  It was phenotyped on the 25 single Yr lines, and genotyped by 19 kompetitive allele-specific PCR-single nucleotide polymorphism (KASP-SNP) markers.  As a result, the 65 progenies were identified as 56 various virulence patterns (VPs), and neither of which was identical to the parental isolate, showed 100% virulence variation.  Compared with the parental isolate, of all progenies, 39 (60.0%) had increased virulence, and 18 (27.7%) had decreased virulence.  All progenies exhibited avirulence to Yr10, Yr15, Yr32, and YrTr1 loci, and avirulence and virulence segregation at the remaining 21 Yr resistance loci.  The results showed avirulence to Yr5, Yr7, and Yr76 (A:V≈3:1) loci is controlled by a single dominant gene, and that to Yr6, Yr25, and Yr44 (A:V≈1:3) loci by a single recessive gene.  However, avirulence to the remaining 15 resistant loci including Yr1, Yr2, Yr3, Yr4, Yr8, Yr9, Yr17, Yr26 (=Yr24), Yr28, Yr29, Yr43, YrSP, Yr27, YrA, and YrExp2, with various avirulence and virulence segregation ratios, is controlled by two genes with different gene effects, indicating complex genetic traits of the parental isolate.  Totally, 65 dissimilar genotypes detected among progenies using overall molecular markers, by which a linkage map was constructed, with a genetic distance of 441.0 cM.  Interestingly, although the parental isolate was avirulent to Yr5, but 17 progenies showed virulence, showing the change of pathogenicity from avirulence to virulence at this resistance locus.  It was for the first time to report that progenies with virulence to Yr5 produced sexually from avirulent parental isolate at this resistance locus.  To our knowledge, this study offers an insight into inheritance, sexual reproduction and virulence variation of P. striiformis f. sp. tritici in Xinjiang, facilitating us to understand evolution of the rust pathogen in this region and accounting for Xinjiang population distinguished form other inland populations.  Additionally, it is necessary to further confirm the roles of sexual reproduction in the emergence of new races and affecting population genetic diversity of P. striiformis f. sp. tritici under natural conditions in Xinjiang.  

Based on the practical experience of the winter wheat-summer maize multiple cropping model in Qingyang City,Gansu Province, the key high yield cultivation techniques were systematically summarized and its economic benefits were analyzed in this paper. For variety selection, early-maturing varieties with strong stress resistance and a requirement of ≤2 100℃ effective accumulated temperature, such as Kewo028, KWS7340, and KWS6333, were preferred. In terms of production management, emphasis was placed on timely land preparation and sowing with haste, with sowing should be completed by June 30 at the latest; integrated mechanical sowing combining “no-tillage, fertilization, and seeding” was adopted. Planting density was optimized, and 2-3 seeds per hole was recommended for mechanical sowing. Scientific fertilization was implemented, with a one-time application of 20 kg of pure nitrogen and 12 kg of pure phosphorus per 667 m⊃2;. Weed control was conducted via unmanned aerial vehicle (UAV) spraying of herbicides such as 6 g of 30% topramezone and 180 g of 25% mesotrione-terbuthylazine per 667 m⊃2;. Integrated pest and disease control was achieved through a combination of agricultural (selection of pest-and-disease-resistant varieties and implementation of scientific crop rotation), biological (introduction of natural enemies, etc.), and chemical (application of 7% cyantraniliprole suspension concentrate, 75% trifloxystrobin-tebuconazole water-dispersible granules, etc.) measures to manage pests and diseases including Spodoptera frugiperda and Setosphaeria turcica. A three-level prevention and control system consisting of “meteorological early warning, field monitoring, and emergency response” was established to reduce the risk of meteorological disasters, and timely harvesting was carried out during October 20-30. Economic benefit analysis shows that suitable varieties (Kewo028) can achieve a net profit of 320 yuan/667 m⊃2;. At present, the disaster resistance and mitigation capacity of this model need further improvement, and the technical systems such as agricultural machinery adaptation, agronomic integration, and variety breeding also require continuous refinement. To this end, it is necessary to strengthen the construction of agricultural infrastructure and promote the transformation of high-standard farmland; establish a technical service network to facilitate technology transfer. This study provides a reference for similar crop cultivation in relevant regions.

To reveal the spatial differentiation patterns of yield ratios (PIR, PDR, IDR) among different cultivated land types (paddy field, irrigated land, dryland) for winter wheat and summer maize in Henan, the paper analyzed their coupling mechanism with cultivated land grade and terrain conditions, so as to provide a scientific basis for improving cultivated land irrigation use efficiency and optimizing cultivated land resources management. Based on the cultivated land grade database and township-level grain yield data (2031 towns for winter wheat, 2000 towns for summer maize) of Henan, 8 grading factor indicator zones (excluding the Huainan hilly and mountainous region) were selected as the study objects. The yield ratios were calculated using the area-weighted method. The correlation between cultivated land grade and IDR was fitted using trend analysis, and the spatial differentiation characteristics of IDR were interpreted with terrain classification. The results showed that: (1) at the indicator zone level, the volatility of yield ratios for both winter wheat and summer maize followed the pattern: IDR>PIR>PDR. The IDR showed the largest difference (0.55 for winter wheat and 0.48 for summer maize), indicating that the yield difference between irrigated land and dryland was most significantly affected by regional conditions; (2) at the provincial level, the regional differences in yield ratios were reduced and the patterns were weakened due to the area-weighted average neutralizing regional heterogeneity; (3) a positive correlation was found between cultivated land grade and IDR (the better the cultivated land quality, the larger the IDR). The sensitivity of winter wheat IDR to cultivated land quality (absolute slope value 0.030) was higher than that of summer maize (0.011); (4) under the same cultivated land grade, terrain ruggedness was positively correlated with IDR (IDR in mountainous and hilly areas was higher than that in plains and basins). The yield ratios among cultivated land types of winter wheat and summer maize in Henan exhibit the significant regional differences and scale dependence. Winter wheat shows a stronger dependence on irrigation conditions. It is suggested to prioritize enhancing the irrigation guarantee capacity for winter wheat on high-quality cultivated land; while for low-quality cultivated land, it requires synergistic improvements in both soil conditions and irrigation infrastructure to improve grain yield.

Wheat flour is one of the important raw materials in food processing, and its processed steamed bread is the staple food for people in the north. In order to analyze the relationship between the rheological fermentation characteristics of wheat dough and the processing quality of steamed bread, this study used 30 different gluten wheat varieties, such as ‘Shiyou 17’, ‘Shiyou 20’ and ‘Shimai 19’, as materials, and analyzed them using correlation and path analysis methods. The results showed that the hardness, gumminess and chewiness of TPA of steamed bread had extremely significant negative correlation with dough maximum expansion height (Hm), dough height at the end of the trial (h) and the time when dough began to leak carbon dioxide gas (Tx). The adhesiveness of steamed bread was extreme significantly positively correlated with the ratio of retained volume to total volume of released gas (Vr/Vt), extreme significantly negatively correlated with the time to reach the curve peak (T₁), Vt and Vr, and had positive correlation with (Hm-H)/Hm. The adhesiveness, springiness, cohesiveness, and resilience of steamed bread demonstrated a highly significant positive correlation with Vr/Vt and a highly significant negative correlation with Vt. The parameters that had a positive effect on the steamed bread sensory score from large to small were: P value (maximum pressure P value), Ie value (elasticity index), weakening degree, flour quality index, T₁ and Vr, and the parameters that had a negative effect were W value (deformation energy) and ratio of P/L (curve shape ratio).

【Objective】Low molecular weight gluten subunits (LMW-GS) are crucial components in determining the end-use quality of common wheat. Elucidating the diversity and impact on flour quality of LMW-GS is essential for genetic improvement in wheat.【Method】A collection of 421 Shanxi wheat accessions was utilized to identify allelic variations in the Glu-A3 and Glu-B3 using 18 specific markers. The polymorphism, genetic diversity, and cluster analysis of these alleles in Shanxi wheat was then conducted. Additionally, the effects of these alleles on the physicochemical quality, farinograph and extensograph properties, as well as dough viscosity characteristics of flour were investigated.【Result】The allelic variations in the Glu-A3, by frequency, are Glu-A3b, Glu-A3c, Glu-A3g, Glu-A3d, Glu-A3f, Glu-A3a, and Glu-A3e. At the Glu-B3, the identified allelic variations are Glu-B3g, Glu-B3a, Glu-B3d, Glu-B3i, Glu-B3b, Glu-B3h, Glu-B3e, Glu-B3c, and Glu-B3f. The genetic distance range for Shanxi wheat is 0.000 to 0.667, with a mean value of 0.253. It was observed that cultivars exhibited a greater genetic distance than landraces. Geographical distribution and irrigation/dryland types also influenced the genetic diversity of LMW-GS. Correlation analysis revealed that six LMW-GS allelic variations significantly correlated with five physicochemical quality traits. Glu-A3b, Glu-A3d, and Glu-B3i had negative effects on protein content, while Glu-A3b and Glu-B3i had positive effects on starch content. Glu-A3d and Glu-B3i showed positive effects on fiber content, and Glu-B3i increased the whiteness value of flour by 2.10%. Fifteen LMW-GS allelic variations were identified as significantly correlated with 12 processing quality traits, with Glu-A3e and Glu-B3d increasing extensibility by 36.71 and 19.91 mm, respectively. Glu-A3a, Glu-B3b, and Glu-B3e had positive effects on improving development time, stability time, stretch area, extension resistance, stretch ratio, and farinograph quality. Five LMW-GS subunits were significantly correlated with dough viscosity characteristics, with Glu-A3a increasing peak viscosity by 183.19 cp and the pad abort value by 67.79 cp.【Conclusion】LMW-GS alleles in Shanxi wheat exhibited high polymorphism. Geographical distribution and accession types affected the genetic diversity at the Glu-A3 and Glu-B3 loci. Six, fifteen, and five LMW-GS allelic variations were identified as showing significant correlations with flour physicochemical, processing quality, and dough viscosity traits, respectively.

Based on the geographical location and climatic characteristics of Tianmen City, Hubei Province, the efficient and high quality cultivation model of watermelon and cauliflower intercropping with wheat was explored and summarized. Through rational crop sequencing, the model achieved orderly coordination of the three crops: wheat was sown from late October to early November and harvested in early to mid-May of the following year; watermelon was grafted and nursery-raised from late March to early April, transplanted in late April, and harvested from late June; cauliflower was nursery-raised in mid-July, transplanted in early to mid-August, and harvested in mid-to-late October. The key cultivation techniques for watermelon and cauliflower were emphasized. For watermelon cultivation, high quality disease resistant varieties (Lyushang, Meidu) were selected, and grafting using pumpkin rootstocks was adopted. Before transplanting, sufficient base fertilizer was applied, and ridging and film mulching were implemented. Vine management during wheat harvesting was coordinated to avoid damage. Two-vine pruning was applied, and vines were timely pressed. Irrigation and topdressing were carried out according to growth stages. For pest and disease control, agricultural methods (selecting resistant varieties, rational crop rotation), physical methods (hanging insect traps), and biological methods (spraying Bacillus thuringiensis) were prioritized, supplemented scientifically with low-toxicity chemical agents (25% azoxystrobin). Harvesting was conducted at the appropriate time, and field sanitation was maintained. For cauliflower cultivation, an efficient technology system centered on the “five modernizations” (intensive seedling raising, mechanized operations, integrated water-fertilizer management, green pest control, and post-harvest commercial handling) was adopted. Suitable disease-resistant varieties (Taisong 65 day) were selected, and intensive seedling raising in plug trays was implemented. Mechanized operations were applied for land preparation, ridging, and fertilization. Integrated water-fertilizer management was implemented during the growth period, and leaf folding for shading during the curd stage ensured quality. Green pest control principles were followed, combining agricultural, physical, and biological methods, supplemented with efficient and low-toxicity chemical agents (80% ethylicin, 25% azoxystrobin). Harvesting was performed when curds were compact, and straw was returned to the field. This model effectively improved the multiple cropping index and resource utilization efficiency, beneficial for the green and high quality development of agriculture.

The paper aims to address the issues of wheat variety selection and corresponding pest and disease control in different regions. Firstly, a data corpus with 3481 wheat variety data and 312 pest data was constructed. According to the characteristics of the corpus, the knowledge system model layer of wheat variety and pest control was defined in a fine grained manner. Secondly, the comparative advantages of Bert and Word2vec word - embedding models on small and medium-sized datasets were verified on public datasets and constructed datasets respectively, and then the attribute knowledge of wheat varieties and pests was extracted by using models suitable for each scale datasets. The experimental results showed that the F₁ value of Bert-BiLSTM-CRF model was 0.1499 higher than that of Word2vec-BiLSTM-CRF model in breed datasets. Word2vec-BiLSTM-CRF model was superior to Bert-BiLSTM-CRF model in pest datasets.

To gain in-depth insights into the research progress and cutting-edge trends of high-gluten wheat in the fields of agriculture and food science, a visual analysis was conducted using CiteSpace 6.3.R1, based on the 2014-2024 literature data retrieved from the CNKI database (1496 papers) and Web of Science (WOS) database (3661 papers). The results showed that from 2014 to 2024, the number of publications on high-gluten wheat in the CNKI and WOS databases exhibited a trend of first increasing and then decreasing. The two databases reached their peak publication outputs in 2020 (218 papers for CNKI) and 2021 (514 papers for WOS), respectively, and the global research enthusiasm shifted from a phase of rapid growth to an adjustment period. China ranked first in terms of publication quantity with 1012 papers (accounting for 27.6% of the total publications in WOS) and established close collaborative networks with countries such as the United States, India, and Italy (collaborating with more than 30 countries), which demonstrated robust scientific research vitality. Within China, a tight-knit academic community was formed, represented by scholars including Zhao Guangcai and Chang Xuhong, who made outstanding contributions to the research on high-gluten wheat. Institutions such as the Chinese Academy of Agricultural Sciences and Henan Academy of Agricultural Sciences achieved high academic standards in this field. In the WOS database, scholars such as Gao Xin, Zhu Kexue, Ma Sen, and Arendt Elke K focused their research on quality molecular breeding, wheat stress-resistant genes, wheat adaptability, flour processing, and nutritional health. Meanwhile, collaborative alliances were formed among institutions including Northwest A&F University, the Ministry of Agriculture and Rural Affairs of the People's Republic of China, Murdoch University, and the Chinese Academy of Agricultural Sciences. Keyword analysis revealed that variety breeding, quality improvement, high-yield and high-efficiency cultivation, and flour processing remained consistent research hotspots. The research content shifted from early cultivation techniques to processing quality and comprehensive evaluation, and the research focus extended to food processing and nutritional health. In conclusion, the research on high-gluten wheat boasted broad prospects and great application potential. It was suggested that in the future, efforts should be directed toward strengthening interdisciplinary research, advancing genetic improvement and molecular breeding technologies, expanding its application in food processing, and developing food products with health benefits to meet market demands.

Thinopyrum ponticum (2n=10×=70), a wild relative of common wheat (Triticum aestivum L.), is considered an invaluable genetic resource for wheat improvement due to its abundance of genes conferring resistance to biotic and abiotic stresses.  This study focused on the CH97 line, derived from the BC₁F₇ progeny of a cross between wheat cv. 7182 and Th. ponticum.  Cytological evidence showed that CH97 has 42 chromosomes, forming 21 bivalents at meiotic metaphase I, with the bivalents subsequently separating and moving to opposite poles during meiotic anaphase I.  Through a combination of fluorescence in situ hybridization (FISH), genomic in situ hybridization (GISH), multicolor GISH (mc-GISH), and liquid array analysis, it was determined that CH97 comprises 40 wheat chromosomes and two alien chromosomes from the E^e genome of Th. ponticum, featuring the absence of a pair of 5D chromosomes and variations in 1B, 6B, and 7B chromosomes.  These findings confirm that CH97 is a stable wheat-Th. ponticum 5E (5D) alien disomic substitution line.  Inoculation experiments revealed that CH97 exhibits high resistance to wheat powdery mildew and stripe rust throughout the growth period, in contrast to the highly susceptible common wheat parent 7182.  Compared to 7182, CH97 displayed improvements in thousand-kernel weight and kernel length.  Additionally, utilizing specific-locus amplified fragment sequencing (SLAF-seq) technology, chromosome 5E-specific molecular markers were developed and validated, achieving a 33.3% success rate, facilitating marker-assisted selection for disease resistance in wheat.  Overall, the CH97 substitution line, with its resistance to diseases and improved agronomic traits, represents valuable new germplasm for wheat chromosome engineering and breeding.

To improve the yield and quality of colored wheat in southwest Shandong, this study explored the effects of different proportions of organic fertilizer instead of chemical fertilizer nitrogen on dry matter accumulation, yield and quality of colored wheat, aiming to clarify the optimal fertilization scheme for colored wheat in this area. A field experiment was conducted from 2022 to 2023, to systematically study the effects of no fertilizer (CK), single chemical fertilizer (CF), 25% (M25), 50% (M50) and 75% (M75) organic fertilizer replacing nitrogen fertilizer on dry matter accumulation, grain yield, quality of two colored wheat varieties (A1 ‘Shannongzimai 1’ and A2 ‘Jilanmai 1’). The results showed that compared with CK treatment, different fertilization treatments could increase grain yield, quality and the dry matter accumulation of wheat at wintering, regreening, flag-picking and maturity stages to varying degrees. In term of dry matter accumulation at the maturity stage, there was no significant difference between organic fertilizer and CF in A1 cultivars, and M25 was the highest; compared with CF, both M25 and M50 were much higher in A2 variety, and there was no significant difference with M75. The variation law of grain yield of the two varieties under different fertilization treatments was basically the same, with the increase of the proportion of organic fertilizer replacing chemical fertilizer, grain yield were gradually decreased. Compared with CF, M25 and M50 treatments could increase the grain yield, with a range of 1.01% to 3.76% for variety A1 and 3.48% to 5.0% for variety A2. M75 treatment reduced grain yield. In M25 treatment, the crude protein content of the two varieties increased by 3.14% and 1.81%, the content of wet gluten increased by 1.70% and 2.46%; the ductility increased by 1.10% and 0.79%, respectively. With the increase of organic fertilizer replacing nitrogen fertilizer, the crude protein content and wet gluten content decreased, volume weight and the ductility of A2 variety were no significant difference. In summary, M25 treatment could effectively improve grain yield, quality of colored wheat in the wheat planting area of southwest Shandong.

To explore the response characteristics of spring wheat developmental stages to drought stress and its physiological regulatory mechanisms, and to scientifically address the challenges posed by climate change, ‘Dingxi New 24’ was selected as experimental material, and methods such as curve fitting and correlation analysis were employed to analyze the change characteristics in soil moisture, crop developmental stages, and physio-ecological parameters under two drought stress conditions: field and pot experiments. The results showed that: (1) under continuous drought stress, the booting, heading, and flowering stages of field wheat were shortened by 1 day, 1 day, and 8 days, respectively, while the milk stage was extended by 5 days. The heading stage of potted wheat was delayed by 2 days, and the flowering stage was shortened by 6 days. (2) The soil moisture stress factor, constructed based on exponential and linear models, could effectively simulate the developmental stages changes caused by drought stress. (3) When the soil relative humidity was between 45% and 53%, the photosynthetic physiological parameters and leaf water status indicators exerted a reverse regulatory effect on the advancement of the jointing-to-flowering stages in field wheat. When the soil relative humidity dropped below 45%, both factors then synergistically promoted an earlier milk stage. For potted wheat subjected to drought starting from the booting stage, all leaf physiological indicators worked together to prolong the heading stage. These findings provide a scientific basis for the dynamic monitoring, prediction, and impact assessment of drought in spring wheat in semi-arid regions.

In this paper, the effects of different foliar fertilizer on photosynthesis, yield composition and quality of dryland wheat were studied to provide reference for high quality and efficient wheat cultivation. A field experiment was carried out in Songming, Yunnan Province in 2022, using the newly approved wheat varieties ‘Yunmai 80’ and ‘Yunmai 83’ as the research materials. Five foliar fertilizer treatments (potassium dihydrogen phosphate, brassinolide, sodium nitrate, yield-increasing agent and their mixture) and water control (three replicates) were set up, spraying at the early heading stage according to the instructions. The results showed that the net photosynthetic rate of ‘Yunmai 80’ was higher than that of ‘Yunmai 83’ under different foliar fertilizer treatments, and the difference of net photosynthetic rate between the two varieties was significant to extremely significant. The highest net photosynthetic rate was observed in KH₂PO₄ treatment, while the lowest was in auxin treatment. The chlorophyll content of ‘Yunmai 83’ was slightly higher than that of ‘Yunmai 80’, and the overall chlorophyll content decreased under the treatment of foliar fertilizer. Without considering the variety factor, foliar fertilizer spraying significantly affected the number of fertile spikelets, degenerate spikelets and effective spikelet, and had little effect on the number of grains per spike and yield. However, all foliar fertilizers increased wheat yield. KH₂PO₄, brassinolactone, sodium complex nitrate and augmentol increased the yield by 10.02%, 9.56%, 9.35% and 15.11% when applied individually. In contrast, foliar fertilizer KH₂PO₄ + brassinolactone + sodium nitrate + augmentol mixed spraying led to a yield reduction of 6.74%. For ‘Yunmai 80’, the yield increases were 19.01%, 10.77%, 9.66% and 23.59% for the individual foliar fertilizers, with a 1.98% increase for the mixture. For ‘Yunmai 83’, the yield increases were 1.40%, 8.40%, 9.06% and 6.93% for the individual foliar fertilizers, with a 15.10% yield reduction for the mixture. Foliar spraying generally reduced the protein content and increased the hardness index. However, mixed spraying increased the protein content and decreased the hardness index. The effects on water absorption, stable time, wet gluten content and sedimentation value varied due to differences between varieties, with both positive and negative effects observed for foliar fertilizers. In summary, spraying single foliar fertilizer at heading stage can promote wheat growth and development, increase yield and improve quality indicators by enhancing photosynthesis. In this study, the comprehensive effects of several common foliar fertilizers on Yunnan dryland wheat were systematically compared for the first time. The results emphasized that the appropriate foliar fertilizer should be selected according to the variety characteristics and avoid blind mixing. This study provides an important theoretical and practical basis for the precise foliar fertilization management of dryland wheat in Yunnan under winter and spring drought conditions, which is helpful to improve the drought resistance, yield and quality of regional wheat production.

【Objective】Wheat starch mainly consists of amylose and amylopectin. Long-term consumption of refined flour products increases the risk of chronic diseases such as diabetes, whereas consuming flour with a high content of resistant starch has a positive effect on regulating blood glucose levels. Given the generally positive correlation between resistant starch and amylose, increasing the amylose content in wheat germplasm has become a goal for quality improvement breeding research. 【Method】Four gene fragments of starch branching enzyme (TaSBEIIb) were selected to successfully construct a high-efficiency dsRNA expression vector. A gradient optimization based on culture medium components significantly enhanced dsRNA yield. Based on this, the effects of naked dsRNA and dsRNA encapsulated with the nanocarrier hydroxypropyltrimethyl ammonium chitosan chloride (HACC) on wheat starch metabolism were explored through foliar spraying. Utilizing a wheat seedling culture system, the impact of dsRNA spraying on the amylose content in wheat seedlings and the expression of starch-related genes was observed. Furthermore, a field trial analyzed the effects of dsRNA spraying on the amylose content in mature wheat grains. The protective effect of chitosan quaternary ammonium salt-coated dsRNA and its influence on amylose content in mature wheat grains were also investigated. 【Result】Four recombinant plasmids (pRNAI-TaSBE1-pRNAI-TaSBE4), expressing dsRNA were successfully constructed. The optimized fermentation medium increased the dsRNA yield from 26.54 mg·L^-1 to 50.65 mg·L^-1, representing a 91% increase compared to the initial medium. Spraying dsRNA interfered with the expression of the target genes, with the highest interference efficiency observed on day 7 for the TaSBEIIb1 fragment. After interference with the four fragments, the expression of TaSBEIIb was reduced by an average of 47.73%. Additionally, the interference of TaSBEIIb affected the expression of other genes in the starch synthesis pathway, including TaSSII, TaSSIV, and TaSBEIIa1 with peak interference efficiencies occurring on days 3, 7, and 3, respectively. Their expression levels decreased by an average of 54.53%, 59.94%, and 47.64%. The 2023 field trial indicated that spraying naked dsRNA increased the amylose content in wheat grains by 17.2%-36.5% after 7 days of treatment, although the effect diminished to 0.2%-8.3% by the maturity stage. In the 2024 field trial, multiple applications of both naked dsRNA and chitosan quaternary ammonium salt-coated raised the amylose content in mature wheat grains from 27.72% to 30.37%, about 10% increase compared to the control. 【Conclusion】Exogenous spraying of TaSBEIIbs-dsRNA effectively increases the amylose content in starch.

【Objective】The smallholder farming model has proven inadequate in meeting the demands of modern agricultural development, with large-scale planting emerging as a crucial pathway for agricultural modernization that facilitates sustainable agriculture development. This study aimed to investigate the current situation of wheat fertilization, irrigation, pest control, yield and economic benefits under different planting scales in Henan Province. Furthermore, it elucidated the impact of scale farming on wheat production, thereby providing a theoretical foundation and practical references for achieving sustainable wheat production. 【Method】A field investigation was conducted in Henan Province to examine the current situation of wheat production and its economic benefits from October 2023 to June 2024. By integrating yield with fertilizer use efficiency, this study systematically analyzed the variations in field management, wheat yield, fertilizer efficiency, and economic performance across different cultivation scales. 【Result】There were significant differences in wheat production among farmers with different cultivation scales. The majority of wheat cultivation areas in Henan Province fell within the range of 6.67-20.00 hm², with the Zhengmai wheat series being the predominant cultivar. The most common seeding rate was 225 kg·hm^-2. The application rates of nitrogen and phosphorus as basal fertilizers were lower in farms with 6.67-20.00 hm² compared with other planting scales. Phosphorus and potassium applied during topdressing were also lower than that of other planting scales ≤33.33 hm². Most surveyed farmers irrigated their fields three times, with the highest irrigation frequency observed in farms ranging from 20.00-33.33 hm². Pesticide use primarily involved insecticides and fungicides, with the highest application frequencies for both observed in the 6.67-20.00 hm² scale. Yields and partial factor productivity of fertilizers were significantly higher in farms sized 6.67-20.00 hm² and 20.00-33.33 hm² than in other scales. Wheat yields in these two groups were significantly increased by 3.57%-20.80%, and by 6.03%-23.67%, respectively, compared with other planting scales. Similarly, partial factor productivity improved by 15.87%-43.02%, and by 10.26%-36.10%, respectively. The output-input ratio was significantly higher in fields ≤6.67 hm² compared with other scales, while the highest net returns were observed at farm sizes ≤33.33 hm². 【Conclusion】Substantial variations in wheat management practices were observed across different farm scales in Henan Province. Comprehensive evaluation of yield, fertilizer partial factor productivity, net returns, and output-input ratio identified 6.67-33.33 hm² as the optimal cultivation scale. Substantial efficiency gaps were observed among large-scale wheat growers, indicating considerable potential for optimization through improved management practices. The adoption of more efficient management measures could significantly enhance the operational efficiency of different planting scales.

In the context of China’s rapid economic development and rapid urbanization, the problem of soil heavy metal pollution becomes more and more serious, and the potential impact on plant growth and ecosystem health has aroused widespread concern in academia. As the main food crop in China, the safe production of wheat is very important to national food security. In this study, the pollution status of farmland soil and the distribution characteristics of heavy metals in various organs of winter wheat at maturity in the rural-urban area of Anyang City were analyzed by field investigation and indoor analysis. The results showed that the contents of Cd, Cr and Pb in farmland soil were 0.87, 8.29 and 16.21 mg/kg, respectively. The single factor pollution indexes of Cd, Cr and Pb were 1.45, 0.10 and 0.03, respectively. The total accumulation of heavy metals Cd, Cr and Pb in wheat plants was 0.75, 29.61 and 7.08 mg/kg, respectively. There were differences in the absorption and transport capacity of different heavy metals in wheat plants. In wheat plants, the enrichment ability of roots to Cd, Cr and Pb was the strongest, and the enrichment ability of grains was relatively weak. The transport capacity of stems to Cd, Cr and Pb was the strongest, followed by leaves and shells, and the transport capacity of grains was the weakest. Correlation analysis showed that there was a significant interaction between the absorption, accumulation and transport of different heavy metals in wheat plants, which affected the overall response of wheat to heavy metals. The overall pollution status of farmland soil in the rural-urban area remained at a good level, and no obvious heavy metal pollution was found.

This paper systematically reviews the technical system of straw returning, the development status of machinery and equipment, and the practice mode of machinery-agronomy integration in rice–wheat annual rotation area, and put forward the prospect of machinery-agronomy integration. Straw returning technology, including crushing rotary tillage, mulching, ploughing and reverse rotary stubble crushing, have been widely promoted in the region, and a series of mechanical equipment such as crushing and returning machines, deep ploughing and returning machines, no-till and mulching seeders, as well as multi-functional integrated machinery have been developed in conjunction. In the process of straw returning, typical models of the machinery-agronomy integration involved “crushing and returning to the field + no-till sowing”“straw crushing + deep loosening and land preparation + wide and narrow row planting”“partial returning to the field + utilization of straw as fertilizer”“straw returning to the field + formula fertilization + comprehensive prevention and control of diseases, pests and weeds”, etc. In terms of key technologies, the coordinated combination of straw crushing by machinery and agronomic mulching control helps ensure the smooth emergence of seedlings, the integration of straw crushing and returning to the field with the application of composting agents and organic fertilizers can promote the recycling of nutrients, through measures such as uniform mulching and optimizing the crop rotation system, comprehensive prevention and control of diseases and pests that may be caused by straw returning to the field can be achieved, the combination of “coarse crushing + fine crushing” with the use of water regulation and microbial bacteria agents is conducive to enhancing the efficiency of humification. The machinery-agronomy integration still faces challenges in practical application, such as insufficient adaptability to terrain, low efficiency in straw treatment, prominent soil compaction problems caused by repetitive mechanical operations, and the possible aggravation of the occurrence of pests, diseases and weeds due to straw mulching and returning to the field. In the future, the following aspects should be improved: develop lightweight, multi-functional and modular agricultural machinery, as well as integrate high-power multi-functional integrated machinery with economical and lightweight machinery; improve the crushing device and accelerate the iterative upgrading of integrated machinery for crushing, turning and sowing; optimize agronomic parameters and conduct systematic research on typical integration models; pay attention to the coordinated cooperation between agricultural machinery, agronomic measures and ecological processes. This study provided reference for the optimization of straw returning technology in rice-wheat rotation area.

Wheat powdery mildew caused by Blumeria graminis f. sp. tritici (Bgt) is an important disease worldwide. Detection of latent infection of leaves by the pathogen in late autumn is valuable for estimating the inoculum potential to assess disease risks in the spring. We developed a new tool for rapid detection and quantification of latent infection of seedlings by the pathogen. The method was based on recombinase polymerase amplification (RPA) coupled with an end-point detection via lateral flow device (LFD). The limit of detection is 100 ag μL^-1 of Bgt DNA, without noticeable interference from either other common wheat pathogens or wheat material (Triticum aestivum). It was evaluated on wheat seedlings for this accuracy and sensitivity in detecting latent infection of Bgt. We further extended this RPA-LFD assay to estimate the level of latent infection by Bgt based on imaging analysis. There was a strong correlation between the image-based and real-time PCR assay estimates of Bgt DNA. The present results suggested that this new tool can provide rapid and accurate quantification of Bgt in latently infected leaves and can be further development as an on-site monitoring tool.

China is one of the four major centers of agricultural origin in the world, where two distinct agricultural systems were established: the rice-based system represented by the middle and lower reaches of the Yangtze River, and the dryland farming system represented by the Yellow River basin. Historical records and archaeological evidence indicate that as early as the Shang Dynasty, oracle bone inscriptions already mentioned crops such as millet (Setaria italica), broomcorn millet (Panicum miliaceum), wheat, rice, and soybeans. During the pre-Qin period, the concept of the ‘Five Grains’ was established, and in The Book of Songs (Shijing), the broader term ‘Hundred Grains’ also appeared as a general reference to food crops. However, it is noteworthy that buckwheat, a crop native to China, has long been absent from these documented grain systems. This omission is inconsistent with the fact that buckwheat is an indigenous Chinese crop with high genetic diversity, significant local variation, and a long history of cultivation and domestication in cold mountainous regions. This study conducts a systematic review of the literature related to the origin, evolution, and spread of buckwheat, integrating recent findings in archaeobotany and genetic diversity analysis. Following internationally accepted principles for identifying crop origin centers, and drawing on historical texts, biological characteristics, and distribution patterns, the study presents comprehensive evidence supporting the hypothesis that southwestern China-particularly Yunnan, Sichuan, Guizhou, and the southern fringe of the Qinghai-Tibet Plateau-is the center of origin, genetic diversity, and domestication for Fagopyrum species. There are 23 species of Fagopyrum identified in China, including three cultivated species-common buckwheat (F. esculentum), tartary buckwheat (F. tataricum), and golden buckwheat (F. cymosum)-and 20 wild species, the majority of which are concentrated in southwestern China. This region is not only the native habitat of the ancestral subspecies of common and tartary buckwheat (F. esculentum ssp. ancestrale and F. tataricum ssp. potanini), but also the area with the richest diversity of Fagopyrum, strongly indicating its status as the origin center. Furthermore, molecular markers and phylogenetic studies confirm close genetic relationships between wild and cultivated buckwheat species in this region, providing key evidence for reconstructing domestication pathways. With advancements in modern research, buckwheat has gained recognition not only for its short growth cycle, broad adaptability, and resilience to poor soils and cold climates, making it suitable for cultivation in remote and mountainous areas, but also for its grain's rich content of proteins, flavonoids, and functional sugar alcohols. As a highly promising functional coarse grain crop, buckwheat is particularly suited to the development of characteristic agriculture in central and western China. It holds significant potential for ecological sustainability, nutritional health, and high-value agricultural development, and is expected to play an important role in China’s national nutrition strategy and food diversity conservation. This study provides theoretical and empirical evidence to support the scientific designation of China as the center of origin and domestication of buckwheat, laying a solid foundation for future work in germplasm conservation, variety improvement, and industrial development.

This study aimed to investigate the effects of nitrogen application rates on chlorophyll content, agronomic traits, and yield of wheat in the Yili River Valley region, and to further elucidate the relationship between agronomic traits and yield. Eight widely cultivated wheat varieties in the region were selected as experimental materials, and three nitrogen levels were established: N1 (180 kg/hm²), N2 (270 kg/hm²), and N3 (360 kg/hm²). Field trials and indoor seed inspection data were integrated with statistical methods, including correlation analysis and grey relational analysis, to examine the relationships among nitrogen application rates, agronomic traits, and yield. The results indicated that nitrogen application had the greatest effect on chlorophyll content during the late grain-filling stage. Within the range of 180-270 kg/hm² nitrogen application, agronomic traits such as plant height, spike number, spikelet number per spike, as well as yield, increased with nitrogen rate, except for grain weight per main spike and 1000-grain weight. Among all traits, spike number exhibited the strongest positive correlation with yield, whereas 1000-grain weight showed a negative but non-significant correlation. The order of grey correlation degree between agronomic traits and yield was main spike length (0.66) > spikelet number (0.62) > plant number (0.58) > aboveground biomass (0.57) = flag leaf SPAD value (0.57) > main spike grain weight (0.54) = plant height (0.54) > effective tiller number (0.52). The order of grey correlation degree between yield components and yield was spike number (0.74) > 1000-grain weight (0.56) > grain number per spike (0.47). Both correlation analysis and grey relational analysis consistently demonstrated that spike number was the most influential factor affecting yield. Under the N2 treatment, chlorophyll content, agronomic performance, and yield of wheat were collectively optimized.

To investigate the effects of rice straw returning on soil fertility enhancement and wheat yield in a rice-wheat rotation system, a field plot experiment was conducted. This study examined the correlations among soil aggregate structure, chemical properties, and wheat yield in the 0-20 cm topsoil layer under different straw incorporation methods and two decomposer treatments: 1/3 straw incorporation (JB), 1/3 straw incorporation with decomposer (JBF₁ and JBF₂), full straw incorporation (JQ), and full straw incorporation with decomposer (JQF₁ and JQF₂). The results showed that, compared with JB, JQ or treatments with decomposer (JBF₁ and JBF₂) significantly increased the proportion of water-stable macroaggregates (>0.25 mm), aggregate stability indicators (R_0.25), mean weight diameter (MWD), and geometric mean diameter (GMD). Simultaneously, JQ or treatments with decomposer (JBF₁ and JBF₂) significantly enhanced soil electrical conductivity (EC), organic matter (OM), total nitrogen (TN), available nitrogen (AN), and available potassium (AK) compared to JB. The increase in soil OM was primarily associated with higher levels of heavy fraction organic carbon (HFOC), light fraction organic carbon (LFOC), and readily oxidizable carbon (ROC). Compared to 1/3 or full straw incorporation alone, applying decomposer increased wheat yield by 16.8%-26.4% and 10.1%-20.6%, respectively, with the JQF1 treatment achieving the highest wheat yield. It was found that under the condition of full returning to the field, the effect of Hubei decomposer (JQF₁) was significantly better than that of Shandong decomposer (JQF₂), which significantly increased the content of >0.25 mm macroaggregates by 8.3% and wheat yield by 5.2% compared with JQF₂ (P<0.05). Correlation analysis indicated that wheat yield was significantly influenced by the proportion of >0.25 mm aggregates, aggregate stability, and the contents of soil OM, TN, and AN. In conclusion, straw incorporation combined with decomposer application achieved a synergistic effect of soil fertility improvement and crop yield increase. Specifically, the content of soil > 0.25 mm macroaggregates, OM and AN increased by 15.2 %, 8.7 % and 10.3 %, and the wheat yield increased by 12.5 %, respectively, compared with JB treatment. Straw incorporation combined with decomposer application is a suitable farming practice for rice-wheat rotation areas.