Using humic acid water-soluble fertilizer produced by Zhongcheng Guolian (Henan) Biotechnology Co., Ltd. as the test material, this study investigated the impact of foliar application of humic acid water-soluble fertilizers on the yield and yield factors of wheat and summer maize under different soil texture types in Shangqiu City. The paired test design was used, and the effects on economic benefits were also analyzed. The results showed that with foliar spraying of humic acid water-soluble fertilizer, the number of grains per ear, 1000-grain weight and both yield and economic benefits significantly increased. Statistical analysis revealed a highly significant increase in yield for both wheat and summer maize. The increase in yield and economic benefit varied across different soil texture types. For wheat, the highest yield increase and lowest yield increase rate occurred on silty soil. The highest increase rate and smallest yield increase were found on sandy soil. The order of net increase in economic benefit was silty soil> loamy soil> sandy soil. For summer maize, the yield increase ranking was silty soil> sandy soil> loamy soil, while the orders of increase rate and net increase in economic benefit were loamy soil> silty soil> sandy soil.

Increasing grain yield (GY) and water use efficiency (WUE) of winter wheat in the Huaibei Plain (HP) is essential.  However, the effects of micro-sprinkler irrigation and topsoil compaction after wheat seeds sowing on the GY and WUE are unclear.  Therefore, a two-year field experiment was conducted during the 2021–2023 winter wheat growing seasons with a total six treatments: rain-fed (RF), conventional irrigation (CI) and micro-sprinkler irrigation (MI), as well as topsoil compaction after seeds sowing under three irrigation methods (RFC, CIC, and MIC).  The two years’ results indicated that MI significantly increased GY compared to CI and RF, which averagely increased by 17.9 and 42.1%, respectively.  The increase in GY of MI was due to its significant increase in the number of spikes, kernels per spike, and grain weight.  Chlorophyll concentration in flag leaves of MI after anthesis stage was maintained higher levels than CI and RF, RF was the lowest.  This was due to the dramatically enhanced catalase and peroxidase activity and lower malondialdehyde content under MI.  Compared with RF and CI, MI significantly promoted dry matter remobilization and production after anthesis as well as its contribution to GY.  In addition, MI significantly boosted root growth, and root activity during grain filling stage was remarkably enhanced than CI and RF.  In 2021–2022, there was no significant difference in WUE between MI and RF, but the WUE of RF was significantly lower than MI in 2022–2023.  However, WUE in MI was significantly improved compared to CI, that averagely increased by 15.1 and 17.6% for the two years.  Topsoil compaction significantly increased GY and WUE under rain-fed conditions due to improved spike numbers and dry matter production.  Overall, topsoil compaction is advisable for enhancing GY and WUE in rain-fed conditions, whereas micro-sprinkler irrigation can be adopted to achieve high GY and WUE simultaneously in the HP.

Integrated agronomic optimization (IAO) adopts suitable crop varieties, sowing dates, planting density and advanced nutrient management to redesign the entire production system according to the local environment, which can achieve synergistic improvements in crop yields and resource utilization.  However, the intensity and magnitude of the impacts of IAO on soil quality under long-term intensive production and high nitrogen use efficiency (NUE) require further clarification.  Based on a 13-year field experiment conducted in Dawenkou, Tai’an, China, we investigated the effects of four cultivation modes on the grain yield, NUE, soil aggregate structure, as well as the fraction of organic matter (SOM) and soil quality, reflected by integrated fertility index (IFI) during the winter wheat maturation period in 2020–2022.  The four cultivation modes were traditional local farming (T1), farmer-based improvement (T2), increased yield regardless of production cost (T3), and integrated soil–crop system management (T4).  As IAO modes, T2 and T4 were characterized by denser planting, reduced nitrogen (N) fertilizer application rates, and delayed sowing compared to T1 and T3, respectively.  In this long-term experiment, IAO was found to maintain aggregate stability, increase SOM content (by increasing organic carbon and total nitrogen of the light fraction (LF) and the particulate organic matter fraction (POM)), and improve SOM quality by increasing the proportions of LF and POM and the ratio of organic carbon to total nitrogen in SOM.  Compared to T1, the IFI of T2, T3, and T4 increased by 10.91, 23.38, 25.55%, and by 17.78, 6.41, 28.94% in the 0–20 and 20–40 cm soil layers, respectively.  The grain yield of T4 was 22.52% higher than that of T1, reaching 95.98% of that in T3.  Furthermore, NUE of T4 was 35.61% higher than that of T1 and T3.  In conclusion, our results suggest that T4 synergistically increases grain yield and NUE in winter wheat, while maximizing soil quality.

The impact of drought stress on crop yield and quality is substantial.  Drought priming during the early growth stage of plants has been shown to improve tolerance to drought stress during the reproductive stage, although its effects on grain quality remains elusive.  This study aimed to investigate the influence of drought priming on starch and protein levels in grains under drought stress during grain filling.  Our results reveal that drought stress results in a reduction in starch content and its constituents, while simultaneously increasing glutenin macropolymers and protein fractons.  Notably,, drought primed plants under drought stress (PD) exhibit mitigated declines in starch content and its components, leading to improvements in starch swelling power and pasting properties.  Additionally, PD results in a slight increase in protein fractions, limiting the overall rise in total protein content compared to drought stress alone.  Collectively, our study underscores the efficacyof drought priming as a strategy to counteract the negative effects of drought stress on grain quality, particularly by minimizing starch losses and restraining protein content elevation.

Frequent drought events especially those occur in the reproductive stages severely restrict global crop productivity.  Moderate drought priming during the earlier growth stages is a promising strategy for plants to resist to recurrent severe drought stress.  However, the underlying mechanisms remain unclear.  Here, we subjected wheat plants to drought priming during the vegetative growth stage and to severe drought stress at 10 days after anthesis.  We then collected leaf samples at the ends of the drought priming, recovery periods, and at the ends of drought stress for transcriptome sequencing in combination with phenotypic and physiological determination.  The drought-primed wheat plant maintained a lower plant temperature, with higher stomatal openness and photosynthesis, thereby resulting in much less 1,000-grain weight and grain yield losses under the later drought stress than the non-primed plants.  Interestingly, 416 genes of which 27 transcription factors (e.g., MYB, NAC, HSF) seemed to be closely related to the improved drought tolerance as indicated by the dynamic transcriptome analysis.  Moreover, the candidate genes showed six temporal expression patterns and significantly enriched in several stress response related pathways such as plant hormone signal transduction, starch and sucrose metabolism, arginine and proline metabolism, inositol phosphate metabolism, and wax synthesis.  These findings illustrate new insights into physiological and molecular mechanisms of the long-term effects of early drought priming to effectively improve drought tolerance in wheat, which proved potential approaches to challenge the increasing abiotic stresses and secure food safety under global warming scenarios.

Reducing nitrogen application rates can mitigate issues such as environmental degradation and resource wastage.  However, it can also exacerbate problems such as wheat floret degeneration, leading to reduced yields.  Therefore, investigating wheat floret degeneration mechanisms under low nitrogen stress and identifying mitigation measures are conducive to achieving high yields and sustainable development.  To investigate the physiological mechanism of low nitrogen stress affecting wheat floret degradation and whether exogenous brassinosteroids can alleviate this stress, three nitrogen application rates (N0, no nitrogen application; N1, 120 kg ha^-1 pure nitrogen; and N2, 240 kg ha^-1 pure nitrogen) and exogenous spraying experiments (N0CK, no nitrogen with water spraying; N0BR, no nitrogen with 24-epibrassinolide (an active brassinosteroids) spraying; and N1, 120 kg ha^-1 pure nitrogen with water spraying) were designed.  The results indicated that low nitrogen stress induced a large amount of reactive oxygen species generation.  Although wheat spikes synthesized flavonoids to combat oxidative stress, their energy metabolism (glycolysis and tricarboxylic acid cycle) and ascorbate-glutathione cycle were inhibited, keeping reactive oxygen levels elevated within the spike, inducing cell death and exacerbating floret degeneration.  Furthermore, brassinosteroids played a role in regulating wheat floret degeneration under low-nitrogen stress.  Exogenous foliar spraying of 24-epibrassinolide promoted energy metabolism and the ascorbate-glutathione cycle within the spike, enhancing energy charge and effectively mitigating a portion of reactive oxygen induced by low nitrogen stress, thereby alleviating floret degeneration caused by low nitrogen stress.  In summary, low-nitrogen stress disrupts the redox homeostasis of wheat spikes, leading to floret degeneration.  Brassinosteroids alleviate floret degeneration by improving the redox state of wheat spikes.  This research provides theoretical support for balancing the contradiction between high yields and sustainable development and is beneficial for the application of low nitrogen in production.

The Rpd3 histone deacetylase complex is a multiple-subunit complex that mediates the regulation of chromatin accessibility and gene expression. Sin3, the largest subunit of Rpd3 complex, is conserved in a broad range of eukaryotes. Despite being a molecular scaffold for complex assembly, the functional sites and mechanism of action of Sin3 remain unexplored. In this study, we functionally characterized a glutamate residue (E810) in FgSin3, the ortholog of yeast Sin3 in Fusarium graminearum (known as wheat scab fungus). Our findings indicate that E810 was important for the functions of FgSin3 in regulating vegetative growth, sexual reproduction, wheat infection, and DON biosynthesis. Furthermore, the E810K missense mutation restored the reduced H4 acetylation caused by the deletion of FNG1, the ortholog of the human inhibitor of growth (ING1) gene in F. graminearum. Correspondingly, the defects of the fng1 mutant were also partially rescued by the E810K mutation in FgSin3. Sequence alignment and evolutionary analysis revealed that E810 residue is well-conserved in fungi, animals, and plants. Based on Alphafold2 structure modeling, E810 localized on the FgRpd3-FgSin3 interface for the formation of a hydrogen bond with FgRpd3. Mutation of E810 disrupts the hydrogen bond and likely affects the FgRpd3-FgSin3 interaction. Taken together, E810 of FgSin3 is functionally associated with Fng1 in the regulation of H4 acetylation and related biological processes, probably by affecting the assembly of the Rpd3 complex.

Organic fertilizers have been widely used in farmland systems for reducing the adverse effects of fertilizer input on farmland environment and crop yield. A field study was conducted from 2022-2023 with the winter wheat ‘Jimai 44’ as the material, and four experimental treatments including 90% chemical fertilizer + 10% organic fertilizer, 80% chemical fertilizer + 20% organic fertilizer, 70% chemical fertilizer + 30% organic fertilizer, 60% chemical fertilizer + 40% organic fertilizer (represented by T1, T2, T3 and T4, respectively) were set, and 100% chemical fertilizer was set as the control (represented by CK) to investigated the effects of fertilizer reduction combined with organic fertilizer on yield components of winter wheat and soil physicochemical properties. The results showed that the content of soil water stability macroaggregates and soil organic matter increased with the increase of organic fertilizer application, and the soil water stability macroaggregates and soil organic matter under T3 and T4 treatment significantly higher than that under CK treatment. Meanwhile, a negatively correlation between soil electrical conductivity and organic fertilizer application was found, and there was no significant difference in soil pH under various fertilization treatments. The yield of winter wheat showed first increased and then decreased trend with the increase of organic fertilizer application, and the highest yield of winter wheat was found under T3 treatment (base fertilizer contained 70% chemical fertilizer + 30% organic fertilizer), the number of grains, the number of ears per hectare, thousand grain weight and grain yield of winter wheat under T3 treatment increased by 3.08%, 1.21%, 5.71% and 10.30% compared to those under CK treatment, respectively. Ultimately, T3 treatment had the best performance in improving the physicochemical properties of soil in wheat fields and increasing the yield of winter wheat based on TOPSIS method of entropy weight method, which can be considered as the recommended fertilization method for winter wheat production in experimental areas.

【Objective】 The excessive application of nitrogen fertilizers has led to ecological pollution and waste of agricultural resources. Developing nitrogen-efficient wheat varieties and improving nitrogen use efficiency are effective approaches for achieving sustainable agricultural development and environmental protection. Screening low-nitrogen-tolerant germplasm resources and identifying genetic loci and candidate genes associated with low-nitrogen tolerance can provide materials and theoretical foundations for breeding nitrogen-efficient wheat varieties. 【Method】 A natural population consisting of 389 wheat varieties was cultivated under high-nitrogen (HN) and low-nitrogen (LN) treatments in 10 field environments. Grain yield per plant (GYP) was measured to calculate the stress tolerance index (STI), thereby enabling the classification of varieties with differential low-nitrogen tolerance. Genome-wide association studies (GWAS) were conducted using 660K SNP array genotyping data to identify stable genetic loci associated with low-nitrogen tolerance. Candidate genes were prioritized through haplotype analysis, expression profiling, and functional annotation. 【Result】 Twelve wheat varieties with strong low-nitrogen tolerance were identified, including Zhongluo 08-1, Jimai 15, Jinghua 2, Kehong 1, Mianyang 19, Jimai 22, Zhenmai 4, Yumai 35, Fengkang 7, Mianyang 11, Jinmai 31, and Lumai 5. Fourteen loci significantly associated with STI were detected, among which four (qSTI1A.1, qSTI3B, qSTI6A, and qSTI7A.2) overlapped with previously reported low-nitrogen tolerance or yield-related QTLs. Notably, qSTI3B-replicated across three environments-was identified as a key locus governing low-nitrogen tolerance. Functional annotation revealed that its candidate gene, TraesCS3B02G042400, encodes an AP2/EREBP (APETALA2/ethylene-responsive element-binding protein) transcription factor. Haplotype analysis showed significant STI divergence among varieties carrying distinct haplotypes, while expression levels of TraesCS3B02G042400 exhibited nitrogen dose-responsive upregulation. 【Conclusion】 Twelve wheat varieties with strong low-nitrogen tolerance were screened. A stable genetic locus, qSTI3B, and a candidate gene, TraesCS3B02G042400, associated with low-nitrogen tolerance were identified.

To elucidate the role of green manure in ameliorating production conditions in wheat fields, a field experiment was conducted from 2018 to 2020 in the Southern Xinjiang region. High-fertility and low-fertility wheat fields were selected, and 4 cropping patterns were established: post-wheat relay-cropped maize (CK), summer fallow (BK), post-wheat planted and incorporated rapeseed (YC), and post-wheat planted and incorporated sunflower (YK). The impacts of green manure on soil enzyme activity and wheat yield under different fertility levels were analyzed. The results showed that incorporating green manure enhanced the activity of certain soil enzymes, while summer fallow had no significant effect on improving soil tillage conditions. For high-fertility wheat fields, the YC treatment increased soil urease and invertase activity by 69.6% and 52.6%, respectively, compared to the CK treatment. The YK treatment increased soil alkaline phosphatase and catalase activity by 72.7% and 82.9%, respectively, compared to the CK treatment. For low-fertility wheat fields, the YC treatment increased alkaline phosphatase activity by 148.4% compared to the CK treatment, while the YK treatment increased soil urease, catalase, and invertase activity by 97.2%, 124.3%, and 84.7%, respectively, compared to the CK treatment. Regarding yield and yield components, no statistically significant differences in wheat yield were observed among the treatments in high-fertility wheat fields (P>0.05). In low-fertility wheat fields, the yields of the BK, YC, and YK treatments were increased by 4.5%, 24.1%, and 28.6%, respectively, compared to the CK treatment. The number of grains per spike and thousand-grain weight were significantly higher in the YC and YK treatments than in the CK treatment (P<0.05). Overall, post-wheat planted and incorporated green manure was beneficial for improving soil tillage quality in low-fertility wheat fields and enhancing wheat yield.

Wheat sheath blight is a common soil-borne disease, occurs at moderate to severe levels annually, affecting wheat production safety, yield, and quality. Based on field survey data of wheat sheath blight occurrence in Xuanzhou District of Anhui Province from 2011 to 2024, its occurrence characteristics, influencing factors, and proposed control measures were analyzed. Field investigations revealed that the initial occurrence period of wheat sheath blight in this region remained stable from mid-to-late February to early March, with no significant differences in severity during the initial stage and mild incidence. Within the same growth stage across different years, the severity of wheat sheath blight varied: during the regreening-jointing stage, the average diseased plant rate showed minor differences across years, whereas more pronounced variations were observed during the full heading and flowering-filling stages. The disease severity was rated as level 2 (moderately light) in most years. In the same year, the disease incidence was mild during the tillering stage but became more severe during the heading-filling stage, with significant vertical expansion. The severity of wheat sheath blight is influenced by multiple factors, including field inoculum load, varietal resistance (main cultivars show no resistance), sowing density (excessive population exacerbates the disease), water and fertilizer management (excessive nitrogen fertilization and insufficient organic fertilizer application increase infection risk), field weeds (reducing basal permeability and weakening wheat stress resistance), and climatic conditions (high humidity environments above 10℃ favor disease development). Targeting these occurrence characteristics and influencing factors, comprehensive control technologies were proposed, including timely sowing (October 25 to November 10), seed dressing with chemicals (single or compound formulations such as difenoconazole), agricultural practices (deep soil tillage, ditch cleaning and drainage, rational planting density, balanced fertilization), biological control (Trichoderma, jinggangmycin, etc.), and chemical control (spraying difenoconazole·propiconazole and other chemicals at the early jointing stage). It provides a reference for the control of wheat sheath blight in Xuanzhou and similar regions.

【Objective】This study aimed to explore the effects of multiple cropping green manure combined with different levels of nitrogen fertilizer on the yield and grain quality of spring wheat, so as to provide the theoretical guidance for the construction of chemical fertilizer reduction and high-quality production mode based on green manure in Qinghai province.【Method】The split plot experiment was carried out in the experimental site of the Academy of Agricultural and Forestry Sciences of Qinghai University from 2023 to 2024. Two planting patterns were set up in the main area: multiple cropping green manure after wheat (W-G) and leisure after wheat (W), and three nitrogen fertilizer levels in the sub-area included: no nitrogen application (N0), nitrogen fertilizer reduction by 30% (N1, 157.5 kg N·hm^-2), and the local custom of nitrogen application (N2, 225 kg N·hm^-2). Wheat yield and grain quality (grain protein content, sedimentation value, formation time, stability time, wet gluten, etc.) were determined.【Result】The grain yield of spring wheat under W-GN1 treatment was 5.5% and 13.4% higher than that under WN1 in 2023 and 2024, and 2.0% and 5.3% higher than that under WN2 treatment, respectively; the biological yield under W-GN1 in 2023 and 2024 was 5.1% and 10.6% higher than that under WN1, and 1.5% and 4.6% higher than that under WN2, respectively. W-G could obtain higher harvest index than W. The compensation effect of multiple cropping green manure was negative under no nitrogen application level, and it was between 2.0%-14.0% under nitrogen application level. The contribution of multiple cropping green manure combined with 30% reduction of nitrogen fertilizer to increasing crop yield was the best. At the same time, W-GN1 could improve grain quality by increasing grain protein content, sedimentation value, formation time and stabilization time. The grain protein content under W-GN1 treatment was 10.62% and 9.48% higher than that under WN1, respectively. The grain sedimentation value increased by 25.05% and 18.13%, respectively. The grain formation time increased by 34.70% and 8.66%, respectively. The grain stability time increased by 41.30% and 13.68%, respectively. Through principal component analysis, it demonstrated that the multiple cropping of green manure after wheat had a more significant promoting effect in grain protein content, sedimentation value, formation time and stability time.【Conclusion】The grain yield and quality of spring wheat were significantly improved by 30% reduction of nitrogen fertilizer (N1,157.5 kg N·hm^-2) combined with green manure after wheat harvest, which could be used as a suitable planting mode and nitrogen application level for improving quality and stable yield of wheat under the condition of reducing chemical fertilizer in Qinghai Province.

To further improve the grain yield per unit area, Guichi District, Chizhou, Anhui Province had carried out demonstration planting of rice and wheat intensive cultivation. Its high yield cultivation techniques and the demonstration results were summarized. The high yield cultivation techniques of rice and wheat intensive cultivation include selecting high yield, stable, and early maturing rice and wheat seeds suitable for local planting according to local conditions to ensure the continuity of rice and wheat crop rotation; taking seed treatment measures such as sun drying, seed selection, seed soaking with pesticides, and seed mixing to improve seed germination rate and prevent diseases and pests during the seedling stage; rice seedlings were raised by stacking and darkening, transplanted with pesticides, while wheat was sown by drones and sprayed with paclobutrazol at appropriate times to improve seedling quality; reasonable dense planting to coordinate group growth; adopting soil testing formula fertilization method, rice field water was managed by alternating dry and wet cycles; timely carry out green prevention and control and unified control measures to prevent and control diseases and pests such as rice blast disease, rice false smut, rice stem borer, and wheat Fusarium head blight. From 2023 to 2024, Weiliangyou 8612 was selected as the rice variety and Yangmai 25 as the wheat variety. A demonstration planting of rice and wheat precision farming was carried out in the research area, achieving high yields of rice (12 631.5 kg/hm²) and wheat (8 685.45 kg/hm²), increased production by 11.65% and 26.25% respectively compared to the previous year,and significantly improving planting efficiency. This article provides a reference for promoting the rice and wheat precision farming model in relevant regions.

To clarify the drought resistance of the main wheat varieties cultivated in Shandong Province and to screen out wheat varieties suitable for dryland cultivation, this study used eight varieties, such as 'Jimai 60', as test subjects. Drought stress was simulated using 20% PEG-6000, and changes in various indicators during the germination period were measured. The membership function method was employed to comprehensively evaluate the relative coleoptile length, relative plumule length, relative radicle length, relative germination rate, relative germination potential, and stress germination index. The results showed that drought stress not only reduced the germination rate of wheat seeds but also inhibited the growth of the plumule, coleoptile, and radicle. The D values of the eight varieties were ranked as follows: 'Jimai 60' > 'Linmai 9' > 'Yannong 999' > 'Shannong 30' > 'Shannong 28' > 'Luyuan 502' > 'Yannong 1212' > 'Jimai 22'. Based on the D values, the eight main wheat varieties were classified into three categories: highly drought-resistant varieties were 'Linmai 9' and 'Jimai 60' ; moderately drought-resistant varieties were 'Yannong 999' and 'Shannong 30'; other varieties were sensitive. 'Linmai 9' and 'Jimai 60' are recommended as suitable varieties for dryland cultivation in Shandong Province.

Agricultural productive services as a crucial lever for integrating small farmers into modern agriculture, facilitate small farmers in achieving economies of scale. Drawing upon survey data from 247 wheat farmers in Shandong Province in 2023, this paper initially employed the Logit model to analyze the influencing factors of the adoption of productive services. Subsequently, it utilized the ISM model to stratify these factors for discussion, thereby delineating the decision-making pathway of outsourcing agricultural productive services from the transaction cost perspective. The empirical results showed that service cognition, organizational convenience, own agricultural tools, wheat yield, agricultural technology training, social network, number of disasters, joining cooperatives and paying attention to grain prices significantly influenced farmers’ outsourcing behavior. The decision-making drivers could be categorized into two distinct pathways: ‘externally-driven’ and ‘internally-driven’ mechanisms. In light of these findings, the study proposed three strategic recommendations: establishing a hierarchical service provider network with precision service mechanisms, enhancing the dual-drive system integrating digital empowerment and institutional coordination, and implementing systematic transformation of production factors.

To advance molecular marker-assisted breeding of high yellow pigment (YP) wheat, this study validated the effectiveness of molecular markers associated with key enzymes (PSY, PDS, ZDS, and LCYE) in the YP biosynthesis pathway and oxidative enzymes (LOX, POD) responsible for flour bleaching. This was achieved by measuring flour YP content in 46 wheat varieties (lines). Functional markers YP7A and YP7B2 at the Psy-A1 and Psy-B1 loci, as well as dominant complementary functional markers LOX16 and LOX18 at the Lox-B1 locus, detected distinct allelic variations across the 46 wheat varieties (lines). The differences in mean flour YP content between these allelic variants reached highly significant levels (P<0.01). However, codominant functional markers YP4B1 and YP4B2 at the Pds-B1 locus showed no significant correlation (P=0.063) between allelic variations and flour YP content. Similarly, codominant functional markers POD3A1 and POD3A2 at the Pod-A1 locus revealed no significant differences in YP content between allelic variants, though Pod-A1a materials (low POD activity) exhibited slightly higher mean YP content compared to Pod-A1b materials (high POD activity). Functional markers YP7A, YP7B2, LOX16, and LOX18 are recommended for molecular marker-assisted breeding of high-yellow pigment wheat, whereas codominant markers YP4B1, YP4B2, POD3A1, and POD3A2 should be applied cautiously in such breeding programs.

To evaluate the potential of Bacillus velezensis SX1302 for the biocontrol of wheat Fusarium head blight, plate antagonism assays were conducted to investigate its inhibitory effects against Fusarium graminearum. Additionally, the impacts of different bacterial culture components (cell suspension, cell-free supernatant, heat-treated cell-free supernatant, and cell lysate contents) on F. graminearum growth and deoxynivalenol (DON) production were examined. The field control effect plant growth-promoting traits and its effects on wheat growth of SX1302 were studied. The results demonstrated that B.velezensis SX1302 exhibited strong antagonistic activity against F.graminearum. The cell suspension effectively inhibited spore germination, mycelial growth, and DON production, with inhibition rates of 100%, 68.75%, and 86.89%, respectively. Field trials showed that, compared with spraying PBS, SX1302 treatment reduced the incidence and disease index of wheat Fusarium head blight by 15.20 percentage points and 65.8% , respectively. In terms of growth promotion, SX1302 was capable of producing indole-3-acetic acid (IAA), solubilizing phosphate, and exhibiting ACC deaminase activity. Compared to sterile water soaking, SX1302 inoculation significantly increased root length, stem height, fresh weight, and dry weight by 24.87%, 31.41%, 44.80%, and 17.91%, respectively. In conclusion, B. velezensis SX1302 possesses dual functionalities, including antagonism against F. graminearum and plant growth promotion, making it a promising candidate for developing microbial biocontrol agents and bio-organic fertilizers.

Cover cropping is a diversifying agricultural practice that can improve soil structure and function by altering the underground litter diversity and soil microbial communities. Here, we tested how a wheat cover crop alters the decomposition of cucumber root litter. A three-year greenhouse litterbag decomposition experiment showed that a wheat cover crop accelerates the decomposition of cucumber root litter. A microcosm litterbag experiment further showed that wheat litter and the soil microbial community could improve cucumber root litter decomposition. Moreover, the wheat cover crop altered the abundances and diversities of soil bacterial and fungal communities, and enriched several putative keystone OTUs, such as Bacillus spp. OTU1837 and Mortierella spp. OTU1236, that were positively related to the mass loss of cucumber root litter. The representative bacterial and fungal strains B186 and M3 were isolated and cultured. In vitro decomposition tests demonstrated that both B186 and M3 had cucumber root litter decomposition activity and a stronger effect was found when they were co-incubated. Overall, a wheat cover crop accelerated cucumber root litter decomposition by altering the soil microbial communities, particularly by stimulating certain putative keystone taxa, which provides a theoretical basis for using cover crops to promote sustainable agricultural development. 

Large grain is a favorable trait for appearance quality and large sink potential in wheat breeding.  A stable QTL QGl.caas-5BS for grain length was previously identified in a recombinant inbred line population from the cross of Zhongmai 871 (ZM871) and its sister line Zhongmai 895 (ZM895).  Here, a BC₁F₆ residual heterozygous line was selected from the cross of ZM871/ZM895//ZM871 population, and six heterozygous recombinant plants were identified in the BC₁F₇ population from self-pollination of the heterozygous line.  QGl.caas-5BS was delimited into an interval of approximately 2.2 Mb flanked by markers Kasp_5B33 and Kasp_5B2 (25.3-27.5 Mb) through phenotyping and genotyping the secondary mapping populations derived from these heterozygous recombinant plants.  Five genes were predicted as candidates of QGl.caas-5BS based on sequence polymorphism and differential expression analyses.  Further mutation analysis showed that TraesCS5B02G026800 is likely the causal gene of QGl.caas-5BS.  A gene-specific marker Kasp_5B_Gl for TraesCS5B02G026800 was developed, and a significant genetic effect of QGl.caas-5BS on grain length was identified in a validation population including 166 cultivars using the marker.  These findings lay a good foundation for map-based cloning of QGl.caas-5BS and provide a breeding-applicable marker for the improvement of grain length in wheat.

Late sowing is a critical factor that hinders achieving high-yield, good-quality wheat under rice-wheat rotation.  Understanding the physiological basis and regulatory pathways that lead to late-sown wheat of high yield and good quality is crucial for developing effective cultivation strategies.  A 2-year field experiment was conducted to investigate the effects of sowing date, nitrogen (N) application rate, and planting density on wheat yield, grain quality, population characteristics, and the underlying physiological factors.  The results revealed significant interactions among the sowing date, planting density, and N application in regulating both yield and quality. Late sowing reduced grain yield primarily by reducing the number of spikes and kernels.  However, the latter was improved by increasing N application and the planting density, thus mitigating the yield losses caused by late sowing.  Moreover, the grain protein content (GPC) and wet gluten content (WGC) increased with delayed sowing dates and higher N rates, but decreased with increased planting densities.  For wheat yields over 9,000 or 7,500 kg ha^–1, the latest sowing date should not be later than Nov. 4 or 15, respectively.  In addition, specific criteria should be met, including a maximum of 1.5 and 1.0 million stems and tillers ha^–1, a maximum leaf area index of 6.7 and 5.5, and a dry matter accumulation (DMA) at anthesis of 14,000 and 12,000 kg ha^–1, respectively. For high-yield, good-quality late-sown wheat, the optimal combination is a 25% increase in the N rate (300 kg N ha^–1) and a planting density of 2.25 million (N300D225) or 3.75 million (N300D375) plants ha^–1 for 10- or 20-day delays in sowing, respectively.  These combinations result in higher leaf net photosynthetic rate; higher activities of leaf nitrate reductase, glutamine synthetase, and grain glutamic pyruvic transaminase; and a lower sugar-N ratio during post-anthesis.

[Objective] Winter wheat yield is crucial for national food security and the standard of living of the population. Existing crop yield prediction models often show low accuracy under disaster-prone climatic conditions. This study proposed an improved hierarchical linear model (IHLM) based on a drought weather index reduction rate, aiming to enhance the accuracy of crop yield estimation under drought conditions. [Methods] HLM was constructed using the maximum enhanced vegetation index-2 (EVI2max), meteorological data (precipitation, radiation, and temperature from March to May), and observed winter wheat yield data from 160 agricultural survey stations in Shandong province (2018－2021). To validate the model's accuracy, 70% of the data from Shandong province was randomly selected for model construction, and the remaining data was used to validate the accuracy of the yield model. HLM considered the variation in meteorological factors as a key obstacle affecting crop growth and improved the model by calculating the relative meteorological factors. The calculation of relative meteorological factors helped reduce the impact of inter-annual differences in meteorological data. The accuracy of the HLM model was compared with that of the random forest (RF), Support Vector Regression (SVR), and Extreme Gradient Boosting (XGBoost) models. The HLM model provided more intuitive interpretation, especially suitable for processing hierarchical data, which helped capture the variability of winter wheat yield data under drought conditions. Therefore, a drought weather index reduction rate model from the agricultural insurance industry was introduced to further optimize the HLM model, resulting in the construction of the IHLM model. The IHLM model was designed to improve crop yield prediction accuracy under drought conditions. Since the precipitation differences between Henan and Shandong provinces were small, to test the transferability of the IHLM model, Henan province sample data was processed in the same way as in Shandong, and the IHLM model was applied to Henan province to evaluate its performance under different geographical conditions. [Results and Discussions] The accuracy of the HLM model, improved based on relative meteorological factors (rMF), was higher than that of RF, SVR, and XGBoost. The validation accuracy showed a Pearson correlation coefficient (r) of 0.76, a root mean squared error (RMSE) of 0.60 t/hm², and a normalized RMSE (nRMSE) of 11.21%. In the drought conditions dataset, the model was further improved by incorporating the relationship between the winter wheat drought weather index and the reduction rate of winter wheat yield. After the improvement, the RMSE decreased by 0.48 t/hm², and the nRMSE decreased by 28.64 percentage points, significantly enhancing the accuracy of the IHLM model under drought conditions. The IHLM model also demonstrated good applicability when transferred to Henan province. [Conclusions] The IHLM model developed in this study improved the accuracy and stability of crop yield predictions, especially under drought conditions. Compared to RF, SVR, and XGBoost models, the IHLM model was more suitable for predicting winter wheat yield. This research can be widely applied in the agricultural insurance field, playing a significant role in the design of agricultural insurance products, rate setting, and risk management. It enables more accurate predictions of winter wheat yield under drought conditions, with results that are closer to actual outcomes.

In order to alleviate the problem of water resource shortage in central and southern Hebei Province, we identified high and stable yield wheat varieties suitable for water-saving irrigation conditions in this wheat region. In this experiment, the 2023 national approved new wheat variety ‘Shimai 34’ and the water-saving control variety ‘Shimai 22’ in Hebei Province were used as experimental materials. Under the condition of one irrigation at jointing stage throughout the whole growth period, comprehensive evaluation of various indicators of ‘Shimai 34’ was conducted by measuring yield, stem morphology indicators, stem strength, and quality related parameters. The results showed that the yield of ‘Shimai 34’ was significantly higher than that of ‘Shimai 22’, with an increase of 7.05%. Morphological analysis showed that the plant height and center of gravity height of ‘Shimai 34’ were 10.17% and 14.78% lower than those of ‘Shimai 22’, respectively. The length of the second internode at the base was 21.39% shorter and the diameter was 15.84% longer than those of ‘Shimai 22’. The stem strength, puncture stem strength, plumpness, and lodging resistance index of ‘Shimai 34’ were 54.95%, 18.10%, 21.34%, and 81.98% higher than those of ‘Shimai 22’, respectively. Further quality testing revealed that the grain hardness, wet gluten, and dry gluten content of ‘Shimai 34’ were superior to those of ‘Shimai 22’. Based on the above data, ‘Shimai 34’ shows better high yield, lodging resistance, and quality than the water-saving control variety ‘Shimai 22’ under water-saving irrigation conditions. ‘Shimai 34’ is expected to become the main wheat variety under the water-saving irrigation in Hebei Province.

In order to explore the accumulation dynamics of deoxynivalenol (DON) in wheat grain under field natural infected conditions and its correlation with disease spike rate, disease index, and percentage of fusarium damaged kernel, the disease spike rate, disease index, thousand grain weight, and percentage of fusarium damaged kernel of susceptible cultivar 'Huaimai 33' and moderately resistant cultivar 'Ningmai 13' from the early filling stage to the delayed harvest stage of 17 days and 15 days had been investigated by field natural infected in 2023 and 2024, respectively. Meanwhile, the DON content in grain had been detected by using ELISA method, and correlation analysis between the DON content in grain and disease spike rate, disease index, and percentage of fusarium damaged kernel had been conducted, respectively. The results showed that DON in grain had accumulated at the early filling stage before the appearance of fusarium head blight symptoms, and the DON content in grain were 237.99 and 182.95 ng/g, respectively. The DON in grain, disease spike rate, disease index, and percentage of fusarium damaged kernel gradually increased during the filling stage, showed slight increases during the dough stage, and tended to be stable during the complete ripe stage. No significant increases were observed during the delayed harvest stage, with no significant differences compared to the complete ripe stage. The thousand grain weight gradually increased during the filling stage, and had basically reached the maximum value by the end of the filling stage. For the same wheat cultivar, positive correlations were found between the DON content in grain and disease spike rate, disease index, and percentage of fusarium damaged kernel. The results of this study provided theoretical basis for the green prevention and control of fusarium head blight and DON in grain, as well as for reducing pesticide application, improving efficiency, and ensuring the quality and safety of agricultural products.

The study aims to screen superior wheat varieties suitable for cultivation under the current context of rapid wheat cultivar renewal and iteration, to provide references for achieving coordinated improvement of yield and quality in rice stubble wheat selection in the Lixiahe region. Using 13 different gluten-type wheat varieties including 'Yanmai 1' as test materials, a single-factor randomized block design was adopted. The grain yield was measured manually and converted to 13% moisture content. The water content, protein content and starch content were determined by near infrared analyzer. Test weight meter and hardness meter were employed to measure grain test weight and hardness. Flour yield was determined after milling and sieving. The wet gluten content and gluten index was determined with a gluten washing instrument according to the method of GB/T 5506.2-2008. The settlement value was determined by AACC 56-61A method. The data were processed and analyzed by SPSS with LSD method. Y1 and Z13 showed the highest yield, which were above 7700 kg/hm² and increased by 12.12% to 25.68% compared with other varieties, while simultaneously meeting national quality standards for medium-gluten wheat: grain hardness ≥60%, test weight ≥770 g/L, protein content ≥12.5%, and wet gluten content ≥26%. N13 demonstrated relatively high yield (6936 kg/hm²) with protein content (12.41%), wet gluten content (24.11%) and sedimentation value (24.32 mL) all reaching national standards for weak-gluten wheat. Other varieties showed moderate yield levels with more complex and overlapping quality indicators. This experiment demonstrates that 'Yanmai 1', 'Zhenmai 13' and 'Ningmai 13' respectively represent high-yielding and high-quality medium-gluten varieties, and stable-yielding high-quality weak-gluten varieties suitable for promotion in this region, laying a solid foundation for coordinated improvement of yield and quality in rice stubble wheat cultivation in the Lixiahe region.

To screen the wheat varieties suitable for planting in the Cd-contaminated farmland of Hebei Piedmont Plain, the field experiment was conducted to study the yield and differential characteristics of cadmium absorption of 48 local main wheat varieties. The results showed that there were significant differences in the yield and the ability to absorb and accumulate Cd among 48 wheat varieties (P<0.05). The yield of ‘Shimai 27’ was the highest (10538.6 kg/hm²), which was 6.33%-42.41% higher than that of other wheat varieties. The yield of ‘Shimai 26’ was the second highest, which was 6.77%-38.51% higher than that of other wheat varieties. The grain Cd contents and enrichment coefficients of ‘Shimai 26’, ‘Zhongxinmai 48’, ‘Malan 6’ and ‘Kenong 2011’ were relatively low, ranging from 0.115 to 0.133 mg/kg and from 0.032 to 0.038, respectively. The Cd transport coefficients of ‘Kenong 2011’, ‘Zhongxinmai 48’ and ‘Shimai 26’ were relatively low, ranging from 0.273 to 0.291. The results of cluster analysis showed that there were 2 varieties of low Cd accumulation, 19 varieties with relatively low Cd accumulation, 3 varieties with low Cd translocation, and 7 varieties with relatively low Cd translocation. By comprehensive evaluation of wheat yield, grain Cd content, enrichment coefficient and transport coefficient, ‘Shimai 26’, ‘Zhongxinmai 48’ and ‘Kenong 2011’ were identified as Cd low accumulation type wheat varieties for safe production in the Cd-contaminated farmland.

To investigate the effects of zinc (Zn) on seed germination and growth of wheat, two cultivars, Shangmai 5226 and Xiaoyan 15, were selected as experimental materials. 6 Zn concentration gradients (ZnSO₄ 0, 50, 100, 200, 400, and 800 mg/L) were set, with 0 mg/L as the control (CK). The germination indicators, growth indicators and physiological indicators of each group were measured. The results showed that in terms of germination parameters, as Zn concentration increased, the germination potential and germination rate of both wheat cultivars first increased and then decreased; at 100 mg/L Zn, Shangmai 5226 and Xiaoyan 15 exhibited higher germination rates and germination potentials. Regarding growth indicators, with increasing Zn concentration, shoot length, shoot fresh weight, and shoot dry weight of both cultivars initially increased and then decreased, whereas root length, root fresh weight, and root dry weight gradually declined. For physiological indices, as Zn concentration increased, chlorophyll content, soluble protein content, soluble sugar content, superoxide dismutase (SOD) activity, and catalase (CAT) activity in both wheat cultivars showed a trend of increasing first and then decreasing. In contrast, peroxidase (POD) activity and malondialdehyde (MDA) content progressively increased, while free proline content first decreased and then increased. In conclusion, Zn concentrations of 50-100 mg/L were conducive to the seed germination and seeding growth of Shangmai 5226 and Xiaoyan 15; Zn concentration exceeding 100 mg/L had a certain inhibitory effect on wheat seed germination.

To promote the technology of replacing chemical fertilizers with organic fertilizers and study its practical effects in wheat production, an experiment was conducted at Yanghu Farm in Sixian, Anhui Province. The experiment included 7 fertilization treatments: no fertilization, conventional fertilization, optimized fertilization, organic and inorganic combined fertilization 1 (organic fertilizer replacing 15% chemical fertilizer), organic and inorganic combined fertilization 2 (organic fertilizer replacing 30% chemical fertilizer), nitrogen substitution 1 (organic fertilizer replacing 15% nitrogen fertilizer), and nitrogen substitution 2 (organic fertilizer replacing 30% nitrogen fertilizer). Wheat yield, quality, economic benefits, and soil physicochemical properties were measured under different treatments. The results showed that both organic and inorganic combination and nitrogen substitution treatments increased wheat yield; beneficial for increasing the total protein and starch content of wheat grains and improving economic benefits; the use of organic fertilizers instead of chemical fertilizers had a promoting effect on the increase of available phosphorus, available potassium, total nitrogen, total phosphorus, total potassium, and organic matter content in soil. Overall, the application effect of replacing 15% nitrogen fertilizer with organic fertilizer (nitrogen substitution 1) is better, resulting in higher wheat yield and economic benefits.

To compare and analyze the effects of phosphorus and potassium migration treatment on the number and weight of grains in different parts of wheat spikelet under late-spring coldness, the varieties Yannong 19 with strong resistance to late-spring coldness and Xinmai 26 with weak resistance to late-spring coldness were used as experimental materials, the 6 treatments were seted up, including room temperature+all basic application of phosphorus and potassium fertilizers (CKR₁), room temperature+split application of phosphorus and potassium fertilizers (CKR₂), 4 ℃+all basic application of phosphorus and potassium fertilizers (T₁R₁), 4 ℃+split application of phosphorus and potassium fertilizers (T₁R₂), -4 ℃+all basic application of phosphorus and potassium fertilizers (T₂R₁), -4 ℃+split application of phosphorus and potassium fertilizers (T₂R₂), and the number of grains and thousand grain weight at different spikelet positions of wheat were measured at maturity. The results showed that the late-spring coldness treatment reduced the number of grains and thousand grain weight at the upper and lower spikelet positions of the wheat varieties tested, with little effect on the grain yield in the middle spikelet positions. The backward shift of phosphorus and potassium significantly increased the number of grains per spike and thousand grain weight of late-spring coldness wheat during the jointing and booting stage, thereby reducing the impact of late-spring coldness on wheat single spike weight. This article provides guidance on preventing and mitigating late-spring coldness in wheat production.

To determine the aerial application effect of 19% picoxystrobin·propiconazole microemulsion on wheat scab, 5 treatments were set up, namely 19% picoxystrobin·propiconazole microemulsion, 48% cyanothiazole·tebuconazole suspension, 30% prothiazole dispersible oil suspension, 30% triazole ether · fluconazole suspension and clear water control treatment (CK). Field aerial application experiments were conducted using unmanned aerial vehicles. Observe the safety of the agent and the phenotypic traits of wheat, calculate the control effect and determine its yield. The results showed that 19% picoxystrobin·propiconazole microemulsion had good safety for wheat growth. After treatment, the wheat leaves were wax-yellow to light yellow, the ears and stalks were wax-yellow, and the maturity appearance was relatively good. 7 days after the second application of the drug, the prevention and control effect was better. The rate of diseased clusters, the control efficacy of diseased clusters, the disease index and the control efficacy of diseased fingers were 0.20%, 95.45%, 0.03 and 95.65% respectively. 28 days after the second administration of the drug, the rate of diseased clusters, the control effect of diseased clusters, the disease index and the control effect of disease fingers were 6.00%, 80.52%, 2.91 and 82.50% respectively, all of which were higher than those in other treatment groups. The yield results indicated that the yield of wheat treated with 19% picoxystrobin · propiconazole microemulsion was 7 146.60 kg/hm², which was higher than that of other treatments. Compared with CK, the yield increase rate was 11.39%. The comprehensive results show that under the conditions of plant protection unmanned aerial vehicles, the application of 19% picoxystrobin · propiconazole microemulsion to control wheat scab has a good control effect, a long persistence period, and a certain yield-increasing effect.

Irrigation methods and nitrogen (N) fertilization modes have a complicated impact on wheat physiology, growth, and development, leading to the regulation of wheat grain yield and quality.  However, the optional water-N combination for drip-irrigated winter wheat remains unclear.  A two-year fieldwork was conducted to evaluate the influences of various N-fertigation and water regimes on wheat post-anthesis grain weight variation, yield, grain NPK content, and grain quality.  The two irrigation quotas were I₄₅ (Irrigation when ETa-P reaches 45 mm) and I₃₀ (Irrigation when ETa-P reaches 30 mm) and the six N application rates were N_0-100 (100% at jointing/booting), N_25-75 (25% at sowing and 75% at jointing/booting), N_50-50 (50% at sowing and 50% at jointing/booting), N_75-25 (75% at sowing and 25% at jointing/booting), N_100-0 (100% at sowing), and SRF₁₀₀ (100% of slow release fertilizer at sowing).  The experimental findings showed that post-anthesis grain weight variation, grain yield, grain NPK content, and grain quality were markedly influenced by the various irrigation scheduling and N-fertilization modes.  The N_50-50 treatment was more beneficial for winter wheat post-anthesis grain weight variation than the N_100-0 and N_0-100 treatments under the two irrigation quotas and during the two seasons.  The highest grain yield of 9.72 and 9.94 (t ha⁻¹) were obtained with the I₄₅N_50-50 treatment in 2020-2021 and 2021-2022, respectively.  The grain crude protein was higher in the I₄₅SRF₁₀₀ treatment during the two seasons.  The I₄₅N_100-0 significantly (P<0.05) enhanced the content of grain total starch by 7.30 and 8.23% compared with the I₄₅N_0-100 and I₃₀N_0-100 treatments, respectively during the 2021-2021 season.  The I₄₅N_100-0 significantly (P<0.05) enhanced the content of grain total starch concentration by 7.77%, 7.62 and 7.88% in comparison with the I₄₅N_0-100, I₃₀N_0-100, and I₃₀N_25-75 treatments, respectively in the 2021-2022 season. Considering the principal component analysis (PCL), the N_50-50 split N-fertigation mode could be an optional choice for farmers during winter wheat production via drip irrigation.

Fusarium graminearum is a fungal plant pathogen which causes Fusarium head blight (FHB), a devastating disease on cereal crops. Here we report that FgPMA1 could be a new target to control FHB by the application of double-stranded RNA (dsRNA) of FgPMA1. FgPMA1 was divided into 6 segments to generated RNA interference (RNAi) constructs (FgPMA1RNAi-1, -2, -3, -4, -5, and -6), and these constructs were transformed in F. graminearum strain PH-1. The expression of FgPMA1 reduced by 18.48%, 33.48% and 56.93% in FgPMA1RNAi-1, FgPMA1RNAi-2 and FgPMA1RNAi-5, respectively. FgPMA1RNAi-1, -2, and -5 mutants inhibited fungal development, including mycelium growth, mycelial morphology, asexual and sexual development, and toxin production. The length of lesions on wheat leaves, wheat coleoptiles and wheat ears were shorter after infection with FgPMA1RNAi-1, -2, and -5 mutants than wild-type PH-1. These results showed that three segments (FgPMA1RNAi-1, -2, and -5) exhibited effective silencing effects. After treatment with 25 ng µL^-1 dsRNA of these segments in vitro, the growth rate of mycelium growth was significant decreased, mycelium became deformed with bulbous structure at the tip, and the mycelium lost the ability to produce conidia in F. graminearum strain PH-1, Fusarium asiacitum strain 2021 and phenamacril-resistant strain YP-1. After application of FgPMA1RNAi-1-dsRNA and FgPMA1RNAi-2-dsRNA to wheat ears, pathogenicity reduced 34.21-35.40%. 

Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is an airborne disease. In China, it frequently develops initially in central Shaanxi and southwestern Gansu, and from there, inoculum spreads to the eastern wheat production regions. Field investigations have suggested that Pst could spread from the west to the east within central Shaanxi and that Gansu could serve as the inoculum source for central Shaanxi, but there is no direct evidence for this hypothetical dispersal route. In the current study, 321 Pst isolates collected from central Shaanxi and Gansu in the 2019-2020 and 2020-2021 winter wheat cropping seasons were genotyped using 23 pairs of KASP-SNP markers. The dispersion among subpopulations was analyzed using several approaches, and overall, the populations were found to exhibit high levels of genetic diversity. There was little genetic divergence (0.05 > F_st > 0) within central Shaanxi. However, significant gene flow (Nm > 4) driven by wind-oriented dispersal from west (Baoji) to east (Weinan) occurred. There was also gene flow among the 4 Gansu subpopulations of Tianshui, Longnan, Pingliang, and Qingyang. Migration of the pathogen occurred between central Shaanxi and Gansu. Migration from Gansu to central Shaanxi was major compared with that from central Shaanxi to Gansu that was minor. Genetic variation occurred among isolates, instead of among subpopulations and within isolates. Linkage disequilibrium revealed that there was strong genetic recombination in the subpopulations from Gansu and central Shaanxi. Therefore, the present study provides molecular evidence that Pst spread from west to east in central Shaanxi and showed that Gansu (especially Longnan and Tianshui) was one of the major origins of the pathogen inoculum of wheat stripe rust in central Shaanxi. The results revealed the west-to-east transmission route of wheat stripe rust in central Shaanxi, being used to guide integrated management of the disease. 

【Objective】 Wheat is a cornerstone of global food security, with its production being pivotal in both China and the international community. With global climate change, the threat of high temperature has become increasingly prominent, posing a significant challenge to wheat cultivation. The strategic identification and selection of heat-tolerant germplasm, coupled with the exploration of genes associated with heat resistance, are crucial steps. These efforts are essential for broadening the genetic diversity of heat tolerance in wheat within China, providing prerequisites for breeding heat-tolerant wheat varieties and ultimately contributing to the safeguarding of our nation’s food security in the face of a warming climate. 【Method】 In this study, a natural population of 331 wheat accessions was utilized, and artificial climate chambers were employed to simulate high temperatures conditions. The heat tolerance of wheat seedlings was assessed by monitoring their survival rate under various durations of treatment, using heat resistance grade as the evaluative metric. Meanwhile, a genome-wide association study (GWAS) was conducted using the 55K SNP chip to identify genetic loci associated with heat tolerance. Expression data from multiple tissues, including roots, leaves under heat stress were analyzed, leading to the selection of genes related to heat tolerance. Subsequently, qPCR validation of candidate genes was performed using the extremely heat-tolerant accession Xinong 889 and the heat-sensitive accession Chinese Spring (CS) as materials. 【Result】 Under high-temperature stress, significant variations in survival rates were observed among different wheat accessions. The extremely heat-tolerant, moderately heat-tolerant, moderately heat-sensitive, and extremely heat-sensitive germplasm accounted for 110, 104, 110, and 7, respectively, representing 33.23%, 31.42%, 33.23%, and 2.12% of the total. Heat-tolerant germplasms, including Xinong 889, Zhengmai 7698, Zhongmai 895, Zhoumai 18, and Fengchan 3, were identified. Through GWAS, a total of 293 SNP loci significantly associated with the 12-hour survival rates (SR) and heat resistance grades (HRG) were detected, with the phenotypic variation explained ranging from 4.40% to 12.46%. Among these, 200 loci were related to the 12-hour survival rates, and 257 were related to the heat resistance grades, with 164 loci identified as the same heat-related loci. Based on significantly associated SNP markers, 313 heat-related genes were predicted. According to gene annotation information and expression data under heat stress, 23 heat tolerance candidates were selected, and after qPCR validation of differentially expressed candidate’s genes, 20 key heat tolerance candidate genes were identified. 【Conclusion】 At the seedling stage, 331 wheat germplasms were identified for heat tolerance. A rapid method was developed for determining the survival rate of wheat seedlings subjected to treatments of varying durations at 45 ℃ to assess their heat tolerance In total, 38 heat-tolerant germplasms and 293 loci significantly associated with seedling heat tolerance were screened. Also, TraesCS1A02G355900, TraesCS1A02G389500, TraesCS5A02G550700, TraesCS5D02G557100, TraesCS6D02G402500 and TraesCS7A02G232500 represented as candidate genes were filtered out.

Wheat is one of the important food crops. Due to global warming and changes in farming patterns, wheat planting and production processes are facing frequent diseases, insects and weeds damage, which pose a major threat to wheat yield and quality. Plant growth regulators, as substances that control plant growth and improve yield and quality, have been widely used in crop production. However, there are relatively few studies on the application of plant growth regulators in wheat production through basal application. To explore the effects of different plant growth regulators on wheat seed germination, seedling growth and yield, this study selected three plant growth regulators of mepiquat chloride, choline chloride and diethyl aminoethyl hexanoate, and used the basal application method to study their effects on wheat. The results showed that the three plant growth regulators had significant (P≤0.05) effects on the germination potential and germination rate of wheat seeds, and had different promotion effects on the growth of wheat seedlings. When wheat was treated with 30 mg/L mepiquat chloride, 20 mg/L choline chloride and 4 mg/L diethyl aminoethyl hexanoate, plant height, root length, stem and leaf fresh weight and root fresh weight were significantly increased. Meanwhile, in the field experiment, the results showed that the treatment of wheat with 75 g (ai)/hm² mepiquat chloride, 30 g (ai)/hm² choline chloride and 30 g (ai)/hm² diethyl aminoethyl hexanoate had a significant effect on wheat seedlings, and the three plant growth regulators had different degrees of improvement on wheat yield.

To screen for efficient and safe pre-emergence soil-applied herbicides suitable for winter wheat fields, an evaluation was conducted on the weed control efficacy and safety of 6 herbicides including 33.8% oxadiazon·flufenacet SC through soil sealing treatment. The results showed that 33.8% oxadiazon·flufenacet SC at 750 mL/hm² demonstrated optimal soil-sealing efficacy in wheat fields, achieving 83.29% weed control efficiency 28 days after treatment. Following subsequent foliar application, it maintained 98.74% weed control rate and 95.09% fresh weight reduction efficacy 98 days post-treatment, significantly reducing early-stage weed populations with prolonged residual activity and good crop safety. 50% pretilachlor SC at 1 875 mL/hm² achieved weed control 82.71% at 28 days, but shorter persistence, with fresh weight control declining to 72.52% at 98 days after foliar treatment. 30% pyroxasulfone·flufenacet SC at 1 200 mL/hm² achieved 78.33% weed control at 28 days, exhibiting longer persistence with 93.66% fresh weight control efficacy at 98 days post foliar treatment. These findings validate that 3 herbicides can be effectively applied as pre-emergence soil treatments in winter wheat fields, demonstrating significant weed suppression without observable phytotoxicity to wheat growth. They exhibit promising potential for extended application in winter wheat cultivation regions such as Shanghai. Rotational use is recommended according to local weed infestation patterns to optimize resistance management and sustained efficacy.

This study aims to screen biocontrol strains against Fusarium graminearum species complex in order to provide new biocontrol resources for the sustainable management of fusarium head blight. A high-efficiency biocontrol strain was isolated from non-agricultural soil samples collected in Badong County, Enshi, Hubei Province, using the dilution plate method with Fusarium graminearum as the indicator strain. The inhibitory effects of the isolate Glarea lozoyensis fermentation broth on mycelial growth and spore germination of F. graminearum were assessed,as well as its degradation rates on deoxynivalenol (DON) and zearalenone (ZEN) in vitro. The strain was identified through morphological characteristics and 18S rRNA gene sequence analysis. Further experiments were conducted to assess control efficacy in field plots. The results showed that a 10-fold dilution of its fermentation broth inhibited F. graminearum mycelial growth and spore germination by 91.98% and 100%, respectively, and achieved 100% degradation rates for DON and ZEN. Morphological characteristics and 18S rRNA gene analysis identified as Glarea lozoyensis (GL), designated GL-P1. Field trial results demonstrated that the fermentation broth of GL-P1 achieved a 71.36% control efficacy against Fusarium head blight, comparable to the fungicide tebuconazole. The above study indicates that Glarea lozoyensis P1 is a biocontrol isolate with promising field efficacy against Fusarium head blight, showing potential for further application and development.

To study the performance of yield and quality traits of wheat germplasm resources and their correlation and path analysis，35 wheat germplasm resources from the middle and lower reaches of the Yangtze River were used as materials in this study. The plant height, spike number, spikelet number per spike, 1000-grain weight and yield were investigated manually, and the wheat quality was determined by Perten DA 7200 near-infrared analyzer. The results showed that the yield range of 35 wheat germplasm resources was 5679.00-6331.50 kg/hm², the effective spike range was 427.50-502.50 million/hm², the growth period range was 195.00-202.00 d, the plant height range was 73.00-93.00 cm, the spikelet number per spike range was 32.60-40.00, and the 1000-grain weight range was 37.00-47.10 g. The correlation analysis of yield traits showed that effective spike, growth period, spikelet number per spike and 1000-grain weight were positively correlated with grain yield (r=0.1707, r=0.0401, r=0.2277, r=0.0299), while plant height was negatively correlated with grain yield (r=-0.0587). Path analysis showed that grain yield increased while effective spike, spikelet number per spike and 1000-grain weight increased. The effect of increasing the number of grains per ear was the greatest (direct path coefficient Py=0.8390), followed by the effect of increasing the 1000-grain weight (direct path coefficient Py=0.6668), and the effect of increasing the number of effective ears was the smallest (direct path coefficient Py=0.4916). In general, the quality traits of 35 wheat germplasm resources were quite different, indicating that wheat has rich diversity, which provides a large selection space for wheat quality breeding and selection of offspring.

叶锈病是危害小麦生产的主要病害之一，栽培小麦广谱高抗叶锈病基因匮乏。小麦-冰草易位系2PT-5具有来自冰草2P长臂对小麦叶锈病广谱免疫的区段。为了准确定位该抗叶锈病基因区段，本研究利用辐照诱导获得的小麦-冰草2P易位系TT-5、TT-3和TT-26分离群体进行叶锈菌接种鉴定，结合基因组原位杂交（GISH）、分子标记检测和基因组重测序对抗叶锈病基因进行物理定位。将抗叶锈病定位区间由原来的82 Mb缩小至9.2 Mb，定位于2P长臂物理位置926.4~935.6 Mb区间。目标区间内注释了64个冰草特异基因，包含6个典型抗病基因，其中有2个编码NLR蛋白的基因和2个编码受体激酶的基因响应叶锈菌的侵染。抗叶锈病基因目标区段的定位，为进一步克隆和解析转移到小麦中的这一广谱抗叶锈病基因奠定了重要的基础。