Saline-alkali stress is one of the abiotic stresses in the process of plant growth and development, which can cause water deficit, changes in cell membrane permeability, metabolic disorders and blockage of protein synthesis in plants, resulting in crop yield reduction or death. Finding effective methods to reduce the harm of saline-alkali stress and strategies to improve the saline tolerance of plants are of great significance to the comprehensive utilization of saline land. In this paper, the latest researches on the damage and adaptive mechanism of plants under saline-alkali stress in recent years were summarized, and the physiological and molecular mechanisms of plants responding to saline-alkali stress were summed up. Furthermore, the physiological mechanisms of plants under saline-alkali stress were analyzed, which were mainly regulated by accumulating osmotic substances, increasing the activity of antioxidant enzymes and ionic compartmentalization, etc., and the molecular mechanisms were mainly regulated by signal transduction, transcription factor regulation and the expression of plant salt-tolerance-related gene, etc. This study pointed out the trends and urgent problems in the development of plant adaptation to saline-alkali environments, with a view to providing a certain theoretical basis for the selection and cultivation of saline and alkali tolerant plants.

Lasioderma serricorne is a common pest in tobacco leaf processing and cigarette production. In order to understand the occurrence and damage characteristics of Lasioderma serricorne and take appropriate prevention and control measures for different scenarios, the research progress on the biological characteristics, damage, monitoring and control methods of Lasioderma serricorne were reviewed. The results showed that the reproductive generation and peak activity period of Lasioderma serricorne varied in different regions. The degree of damage in southern regions was higher than that in northern regions, and the degree of damage in high quality tobacco was higher than that in low quality tobacco. The methods for pest monitoring included manual statistics based on traps, image recognition, and electronic nose monitoring. There were 3 methods for the prevention and control of pest: physical control, chemical control, and biological control. Physical control included low-temperature treatment, high temperature treatment, vacuum conditioning, controlled atmosphere treatment, microwave treatment, and ultraviolet light trapping. Chemical control included fumigation, hormones, chemical insecticides, plant-derived insecticides, and plant essential oils. Biological control included bacterial control, fungal control, parasitic natural enemy control, RNA interference technology, and attractant. The research provides references for the integrated control of tobacco beetle.

To screen the excellent maize varieties suitable for planting in Lingbi County, Anhui province, 34 new corn varieties, such as Kangnongyu 8009, Zhongyu 303 and Siyougu No. 5, were used as experimental materials, growth period and disease resistance were recorded, and agronomic traits, such as plant height, yield and yield traits, such as 100 grain weight were measured and evaluated comprehensively. The results showed that the growth period of each variety was 99-105 days. In terms of resistance, stem rot and small spot of all varieties were grade 1, and some varieties were susceptible to rust and sheath blight. In terms of agronomic traits, plant height was 206.4-267.3 cm, ear position 67.2-103.4 cm, ear diameter 4.3-5.0 cm, row number of spike 12.4-17.6 rows, row number 29.0-36.1 grains, seed yield 88.0%-91.5%. In terms of yield and yield traits, the effective panicle was 67 500 panicles /hm², the 100 grain weight was 25.43-36.42 g, the number of grains per panicle was 378.96-603.94 grains, and the yield was 7 240.50-10 062.75 kg/hm². The 5 varieties of Longding 728, Pudan 12, Jiaxi 100, Weike 985 and Denghai 1875 had the best comprehensive performance and were suitable for planting in the study area and related areas.

To analyze the role of native tree species in forestry ecological construction, native tree species and their characteristics were mainly introduced, their role in forestry ecological construction were analyzed, and application strategies were put forward in 3 aspects: scientific planning and planting, protection and breeding technology, ecological education and public participation. Native tree species had the characteristics of strong adaptability, outstanding landscape value and good economy, and their ecological value and landscape value could bring ecological and economic benefits to the local area，and promote the benign development of forestry ecosystem. Native tree species have obvious advantages in forestry ecological construction, which should be full valued, applied and promoted, and scientifically planned and planted according to their growth habits, and research on the protection and breeding of native tree species resources should be strengthened. The awareness of protecting native tree species and the enthusiasm of participating in forestry ecological construction were enhanced by carrying out ecological education activities. The results provided references for optimizing native tree species selection and promoting forestry ecological construction.

[Purpose/Significance] In recent years, China has been committed to building a strong educational country. As key academic institutions within universities, university libraries play a vital role in disseminating knowledge, nurturing talent, and supporting scholary research. Their development is of great significance in promoting the high-quality development of higher education and realizing the goal of building a strong educational country. This study focuses on the "15th Five-Year Plan" of university libraries. It analyzes the development characteristics of university libraries during the "14th Five-Year Plan" period, explores the opportunities and challenges they face in the new era, and puts forward key points and suggestions for the "15th Five-Year Plan" to promote the transformation and upgrading of university libraries and better support the building of a strong education country. The innovation of this article is that it comprehensively considers various factors and national strategic requirements, and puts forward a relatively systematic and forward-looking development plan for university libraries, which provides a new perspective and practical guidance for the research and practice of university libraries in the new era. It contributes to the improvement of the theoretical research system of library science and promotes the deep integration of library services with teaching, scientific research, and cultural heritage in the digital age. [Method/Process] This study mainly adopts the methods of literature review, data analysis, and case study. By collecting and analyzing a large body of literature on library development policies, strategic plans, and related research findings from home and abroad, it examines the development context and trends of university libraries. At the same time, it analyzes the statistical data of library funds, resource construction, and service development during the "14th Five-Year Plan" period to objectively understand the current situation and problems. In addition, it also studies the development cases and practices of some excellent university libraries at home and abroad to draw on successful experiences. The theoretical basis of this study is drawn from the theory of library science, information science, and the relevant theories of education and cultural development. The empirical basis is mainly based on the actual situation and data of the university libraries in China in the past few years. [Results/Conclusions] During the "14th Five-Year Plan" period, university libraries have not only made certain achievements in smart library construction, digital transformation, and information literacy education, but they also face challenges such as reduced funding and polarized academic evaluation services. In the "15th Five-Year Plan" period, university libraries should seize the opportunities provided by national strategies such as national cultural digitization and educational strength, and focus on eight aspects, including to modernize the management system, strengthen the construction of data infrastructure, promote the construction of digital special collections, support digital intelligence teaching, explore the management and operation of future learning centers, build intelligent service systems supported by artificial intelligence, provide full-cycle support for scientific research, and enhance the reader's experience in the virtual environment. At the same time, it is necessary to pay attention to the evaluation of development results, summarize experience and lessons learned, and continuously optimize the development path. Future research can further explore the specific implementation strategies and detailed operation methods of each plan point, strengthen the cooperation and integration between libraries and other departments in the school, and conduct in-depth research on the application and impact of emerging technologies in libraries.

【Objective】Based on the current “dual carbon” strategic goal, this study aimed to clarify the current characteristics, spatio-temporal pattern and influencing factors of agricultural net carbon sink, so as to provide the important support for accelerating agricultural sink increase and emission reduction.【Method】Based on the scientific reconstruction of the index system, the carbon sink/carbon emission factor method was used to measure and analyze the current situation of China’s agricultural net carbon sink. Then the spatial autocorrelation model was used to discuss the spatial dependence and spatial heterogeneity. Finally, the least-squares method was used to analyze the main factors affecting the change of its intensity. 【Result】From 2005 to 2022, the total amount of agricultural net carbon sink in China was in an obvious upward trend, although there were some interannual fluctuations, and its evolutionary characteristics could be roughly divided into four stages, namely, “continuous rise”, “fluctuating decline”, “rapid rise”, and “slow rise”; the intensity of agricultural net carbon sink was also in an obvious upward trend, with only a slight difference in the trajectory of the evolution, and the difference in its growth rate could be roughly categorized into four stages: “continuous rapid growth”, “slow growth”, “fluctuating ups and downs”, and “slow growth”. 2022, the amount of agricultural net carbon sink had a large interprovincial difference, with Inner Mongolia being the first and Shanghai being the last, and compared with the year of 2005, all the provinces had a significant increase. In 2022, the net carbon sink intensity of agriculture would be the highest in Henan and the lowest in Qinghai, with all provinces showing different degrees of increase compared with 2005. China’s provincial agricultural net carbon sink intensity as a whole showed obvious spatial dependence, but there was also a local spatial clustering phenomenon, more than 70% of the provinces showed obvious spatial clustering characteristics, and the number of provinces located in the high-high clustering and the low-low clustering was approaching. The structure of arable land use, urbanization level, rural residents' income level and the internal industrial structure of agriculture all had a significant impact on the intensity of agricultural net carbon sink; specifically, the higher the ratio of sown area of grain crops, or the higher the urbanization rate, or the higher the income level of rural residents, or the larger the ratio of plantation industry to animal husbandry, the higher the intensity of net carbon sink in agriculture.【Conclusion】The total amount and intensity of China’s agricultural net carbon sink were in a fluctuating upward trend and there were obvious inter-provincial differences. The intensity of China’s agricultural net carbon sink showed obvious spatial dependence and spatial heterogeneity. The intensity of the agricultural net carbon sink was affected by the structure of arable land use, the level of urbanization, the level of rural residents' income, and the structure of the internal industries of agriculture. The measures should be taken to promote the enhancement of sink and emission reductions and to promote the enhancement of agricultural net carbon sink in agriculture, such as establishing a sound policy support system for the development of low-carbon agriculture, strengthening inter-provincial exchanges and cooperation, and increasing financial support for agriculture.

In order to effectively control Huanglongbing (HLB) and reduce the loss of HLB to the global citrus industry, this study reviewed the latest research on the control of HLB in recent years. The results showed that there were no effective agents and disease-resistant varieties. At present, the main methods of controlling HLB include traditional ‘three fast methods’, namely planting disease-free seedlings to control HLB from the sources, preventing and controlling Diaphorina citr to block its transmission path, and digging infected trees to eliminate the infection source; expanded physical control technologies include seedling virus-free, light, steam heat treatment, etc.; multi-directional chemical drug control technologies include antibiotics, nanomaterials, immune inducers and antimicrobial peptides; emerging biological control technologies include various probiotics, microbial preparations, etc. Finally, the application prospect of soil amendment and biological control agent combined treatment of HLB and citrus transgenic disease resistance technology based on genetic improvement was prospected, to provide theoretical basis and practical reference for effective control of HLB.

In recent years, the betel nut planting industry in Hainan Province has developed rapidly. Industrial adjustment and optimization will be the focus of its future development, and a reasonable production area layout is crucial for the sustainable development of the betel nut industry in Hainan. Using regional comparative advantage index and resource endowment coefficient, this study analyzed the spatial-temporal evolution characteristics and driving factors of betel nut production in various cities and counties in Hainan from 2005 to 2022. The results showed that the scale of betel nut cultivation in Hainan had significantly increased, but the yield had significantly decreased. The calculated results of the comprehensive comparative advantage index and resource endowment coefficient showed strong consistency. The central regions of Qiongzhong, Ding'an, Baoting, and Tunchang had stronger comparative advantages in betel nut production, which were key areas for the production layout in Hainan. The leading advantage of traditional planting areas in the east was gradually weakening, while the overall western region lacked comparative advantages but showed a clear upward trend. The production of betel nuts in Hainan presents a pattern of retreating in the east, advancing in the west, and maintaining dominance in the center. The driving factors of spatial-temporal evolution mainly include higher economic benefits, the diffusion of high-yield cultivation and management techniques, and the spread of diseases and pests such as yellowing disease. It is recommended to reasonably adjust the layout of betel nut production, moderately control of planting scale, strengthen socialized services, clarify the regulatory policies for edible betel nut products, and accelerate the transformation and upgrading of the betel nut industry.

Seed shattering is a major factor limiting rice production, and breeding new rice varieties with moderate seed shattering is a key challenge faced by rice breeders worldwide. Rice is the most important cereal crop in China, plays a vital role for national food security. Seed shattering is one of the most important traits during rice domestication, and the abscission zone is the important region to control seed shattering. Compared with wild rice, cultivar has eliminated the seed shattering with partially developed abscission layer. Seed shattering not only has a direct impact on the yield, but also affects the way of its mechanical harvest. In order to breed rice varieties with moderate seed shattering in agricultural production, it is necessary to mine and utilize important seed shattering genes and introduce them into excellent rice varieties for genetic improvement, so as to breed new rice varieties suitable for mechanical harvesting with moderate seed shattering. Several seed shattering genes had been identified by map-based cloning, such as SH4/SHA1, qSH1, OsSh1/ObSH3, and their functional mechanisms had been analyzed. At the same time, new rice materials with moderate seed shattering have been successfully developed through CRISPR/Cas9 gene editing technology, gamma ray mutagenesis technology and gene introduction methods. Seed shattering has an important effect on grain yield and rice harvesting methods, in this paper, we reviewed the methods, physiologic basis, the identification of seed shattering genes and genetic mechanism of seed shattering in rice. At the same time, it is proposed that by using the important genes in excellent rice germplasm resources, could provide reference for exploring the mechanism of rice seed shattering, and breed new rice varieties suitable for mechanical harvesting with moderate seed shattering.

With the continuous improvement in rice cultivation techniques, China has maintained a high rice production level of about 210 million tons over the past decade. Direct-seeding rice cultivation technology, recognized for its efficiency and simplicity, has been favored by Chinese farmers. However, controversies persist regarding direct-seeding rice compared to transplanted rice in national-scale production. Thus, this study employed meta-analysis techniques to quantify disparities in grain yield, economic benefit, rice quality, lodging characteristic, and greenhouse gas emissions between direct-seeding rice and transplanted rice. Our results indicated that direct-seeding rice significantly reduced grain yield by an average of 6.3% relative to transplanted rice, which was main due to the reduced total spikelet (-3.8%) and filled-grain percentage (-1.8%). In different planting systems in China, the yield of direct-seeding rice had significantly decreased compared to transplanted rice, and the direct-seeding rice-induced reductions in yield of single rice (-10.9%) and late rice (-13.1%) were higher than those of middle rice (-4.8%) and early rice (-4.4%). The grain yield reductions for direct-seeding rice were from 10% to 20% in Jilin, Liaoning, Xinjiang, Ningxia, Shandong, Jiangsu, and Zhejiang provinces, meanwhile Heilongjiang and Jiangxi provinces saw reductions of 5% to 10%, but it had no significant effect in other provinces. Direct-seeding rice resulted in comparable net economic return relative to transplanted rice (p> 0.05). Direct-seeding rice reduced milled rice rate (-3.1%) and gel consistency (-3.5%), improved appearance quality (chalkiness percentage and chalkiness degree, which decreased by 25.3% and 22.5%, respectively), whereas no significant effects were observed on nutrition quality and taste value. Direct-seeding rice increased lodging index at base of the first (+12.4%) and third (+10.3%) internodes, but not at the second internode, indicating an increase in risk of lodging relative to transplanted rice. In terms of greenhouse gas emissions, direct-seeding rice fields showed reductions in methane emissions (-42.8%), global warming potential (-36.2%), and greenhouse gas intensity (-41.1%) compared to transplanted rice fields, while promoting nitrous oxide emissions (+29.1%). In addition, a review was recounted on nitrogen utilization and its loss, water and energy use efficiency, and weed incidence. Finally, the recommendations for the future advancement of direct-seeding rice were proposed, main focusing on rice variety breeding, rice cultivation technique optimization, rice planting area layout, as well as policies and services with the goal of technological innovation and regionalized application of direct-seeding rice cultivation technology in China.

Feed efficiency (FE) is a crucial economic trait that significantly impacts profitability in intensive sheep production, and can be evaluated by the residual feed intake (RFI) and feed conversion ratio (FCR). However, the underlying genetic mechanisms that underlie FE-related traits in sheep are not fully understood. Herein, we measured the FE-related traits of 1280 Hu sheep and conducted the phenotype statistics and correlation analysis, the result showcase that there was a large variation for FE-related traits, and RFI was significant positive correlation with average daily feed intake (ADFI) and FCR. Moreover, a genome-wide association study (GWAS) was conducted using whole-genome resequencing data to investigate the genetic associations of ADFI, FCR and RFI. For ADFI and FCR traits, two and one single nucleotide polymorphisms (SNPs) exceeded the genome-wide significance threshold, whereas ten and five SNPs exceeded the suggestive significance threshold. For RFI traits, only four SNPs exceeded the suggestive significance threshold. Finally, a total of eight genes (LOC101121953, LOC101110202, CTNNA3, IZUMO3, PPM1E, YIPF7, ZSCAN12 and LOC105603808) were identified as potential candidate genes for FE-related traits. Simultaneously, we further analyzed the effects of two candidate SNPs associated with RFI on growth and FE traits in enlarged experimental population, the results demonstrated that these two SNPs was not significantly associated with growth traits (P > 0.05), but significantly related to RFI traits (P < 0.05). These findings will provide valuable reference data and key genetic variants that can be used to effectively select feed-efficient individual in sheep breeding programs.

China faces significant challenges of large population and limited arable land resources. In recent years, extreme weather events and ongoing soil degradation have significantly influenced China's agricultural production. With advances in agricultural science and technology, incorporating new materials into agricultural practices has been proven to be an effective strategy for adapting to these developments and environmental shifts. Seaweed extract, specifically seaweed oligosaccharides, are natural active substances derived from seaweed using modern technology. They are employed in agriculture as inducers for plant stress and disease resistance, biostimulants, and fertilizer enhancers. With continuous research progresses on the potential applications of seaweed oligosaccharides in agriculture, significant advancements have been achieved in their study and use. To further systematically understand and apply seaweed oligosaccharides, this paper discussed their sources, classification, and production methods. It summarized their impact on bolstering crop stress and disease resistance, fostering growth and development, and improving nutrient absorption, as well as their effectiveness and mechanisms across various crops. This provided innovative approaches and tools for the sustainable and efficient development of Chinese agriculture in the current context.

The global warming rate and the frequency of extreme high temperature weather are continuously increasing, which could bring catastrophic impacts to rice production. To further clarify the response mechanism of rice to high temperature, this article summarized the research progress in the direction of rice heat damage from high temperature, focused on the impact of high temperature on rice production, and deeply analyzed the physiological (photosynthetic characteristics/antioxidant system) and gene molecular mechanisms of rice’s response to high temperature. We concluded that the heat tolerance characteristics of rice were formed by the interaction between varieties and the environment. Results showed that, the photosynthetic characteristics of rice were influenced by both stomatal and non-stomatal limiting factors, and the antioxidant process was achieved by enhancing the activity of antioxidant enzymes and reducing the content of malondialdehyde; rice improved plant heat adaptability by activating the expression of key genes and stimulating the transduction of heat signals within the plant; by optimizing “Before production-During production-After production” comprehensive management and monitoring system, we could explore innovative cultivation systems, and thoroughly investigate the high temperature defense mechanisms of rice. Therefore, this study aimed to clarify the physiological and molecular mechanisms of rice in response to high temperature stress, providing a scientific reference for future heat-tolerant rice breeding work and improving rice sustainable production.

【Objective】Based on the long-term experiment in the North China Plain (NCP), the differences in soil nutrient and aggregate nutrient distribution between diversified crops and wheat-maize rotation systems were investigated. Additionally, it provided a comprehensive evaluation of soil quality indices (SQI), offering a scientific basis for enhancing soil quality and productivity in the NCP. 【Method】Four diversified crop rotation systems were evaluated, including spring sweet potato-winter wheat-summer maize (Psw-WM), spring peanut-winter wheat-summer maize (Pns-WM), spring sorghum-winter wheat-summer maize (Ps-WM), with winter wheat-summer maize (WM-WM) serving as the control. The soil samples from the 0-40 cm depth were collected during the second rotation in 2022, at the flowering and harvesting stages of winter wheat. The soil enzymes activities, aggregate stability, organic matter, and concentrations of nitrogen, phosphorus, and potassium in soil and aggregates of different sizes (>2.00 mm, 0.50-2.00 mm, 0.25-0.50 mm, and <0.25 mm) were assessed. The SQI for each crop rotation system was then comprehensively evaluated. 【Result】Compared with WM-WM, the three other crop rotations increased soil inorganic nitrogen content. Psw-WM significantly enhanced organic matter in the 0-20 cm layer, total nitrogen in soil aggregates (>2.00 mm, 0-10 cm), and organic matter in soil aggregates (>2.00 mm and 0.50-2.00 mm, 0-10 cm), which also increased cellulase, catalase, and alkaline protease activities. Pns-WM improved organic matter in the 20-40 cm layer and available potassium in soil aggregates (0.25-0.50 mm and >2.00 mm, 10-20 cm), as well as organic matter in soil aggregates (0-10 cm, >2.00 mm and 10-20 cm, >0.50 mm), which also increased sucrase, urease, and alkaline protease activities. Psw-WM improved the stability of 0-10 cm soil aggregates, while Pns-WM improved the stability of 0-30 cm soil aggregates. Both Pns-WM and Psw-WM significantly improved the SQI, with Pns-WM showing a higher improvement than Psw-WM. The path analysis revealed that the average weight diameter (MWD) of aggregates was a direct and significant affecting SQI. It also had a significant indirect positive effect on SQI by influencing inorganic nitrogen. Additionally, the increased organic matter led to a higher proportion of large aggregates, which significantly affected SQI indirectly. 【Conclusion】Legume (peanut) and root crop (sweet potato) rotations with wheat-maize rotations could significantly improve soil quality and enhance the soil nutrient supply capacity in the NCP.

Heterosis is a phenomenon where the offspring of genetically distinct populations exhibit superior vitality, reproductive capacity, and adaptability compared with the average of their parent populations, which is an important genetic resource. Heterosis plays a significant role in modern agriculture, contributing to increase yields and quality of livestock and crops, rapidly improve traits, accelerate the breeding of new varieties, and enhance genetic diversity, thereby efficiently boosting the production of animal husbandry and agriculture while reducing costs. Despite the discovery of heterosis is over a century ago, the elucidation of its genetic basis lags far behind its application in agricultural production. The study of the complex formation mechanism of heterosis is a classic and an active topic in the field of genetics and breeding, but the clear conclusions remain limited. In response to the characteristics of heterosis, scientists have successively proposed various hypotheses for its formation, such as the dominance hypothesis, overdominance hypothesis, and epistasis hypothesis, revealing that the genetic basis of heterosis was non-additive genetic effects. However, these hypotheses are based on the effects of single genes, which are overly idealized and simplistic. Explorations at different levels, such as DNA, RNA, and proteins, have successively discovered the coexistence of multiple genetic effects. Particularly in hybrid crops like rice and corn, the related researches have been continuously identified the loci of heterosis effects, enriched the understanding of the formation mechanism for heterosis in crops, and promoted the transformation of crop breeding technologies, such as precise molecular design breeding. Heterosis is also widely applied in the breeding of livestock and poultry. In developed countries with advanced animal husbandry, over 80% of commercial pork, chicken, and eggs are obtained from hybrid breeds. To efficiently apply heterosis in production for animal husbandry, it is necessary to predict heterosis in advance. New methods, such as the inter- and intra-group phenotypic variance ratio prediction, hybrid heritability prediction, and molecular marker prediction, have been developed to solve the long experimental cycle, environmental sensitivity, and high human and financial costs associated with traditional hybridization experiments for predicting heterosis. However, the accuracy of these prediction methods is limited. Heterosis involves in interaction of multiple levels, and because of the complex genetic background and long breeding cycle, it is still a big challenge for the study of the heterosis formation mechanism and accurate prediction methods. In recent years, the gradual application of sequencing technology has provided a new perspective for understanding the molecular regulatory network of heterosis in livestock and poultry. QTL mapping and genome-wide association study reveal the molecular mechanism of heterosis at the genomic level, and the identified molecular makers are applied in selection and breeding. Combined with multi-omics researches, such as transcriptomics and metabolomics, the key functional genes, variations, and metabolites affecting heterosis can be more precisely located, which facilitate hybrid improvement. This review elaborated the research progress in the formation mechanism and prediction methods for heterosis in the field of livestock and poultry. For looking forward to future, the researches will gradually clarify the complex mechanism of heterosis by integrating multi-omics sequencing data and bioinformatics analysis, in order to identify genes and molecular markers related to heterosis, and innovate new prediction methods, which will provide a more accurate direction for the utilization of heterosis.

Gene-edited crops, the product of the intersection between biotechnology and agricultural science, represent a crucial direction in the development of modern agriculture. With the rapid advancement of the CRISPR-Cas9 system, the scientific research and commercial development of crop trait improvement have gradually shifted towards a “technology-driven” path, which has not only overturned traditional crop cultivation methods but also fundamentally propelled humanity’s exploration of crop research. Nevertheless, the phenomenon of patenting fundamental research tools has sparked widespread controversy within academia and profoundly impacted the sharing and utilization of crop resources. Private entities patenting CRISPR-Cas9 technology restrict other researchers and farmers’ opportunities to explore and harness genetic resources. This practice not only hinders scientific progress but also violates the fundamental consensus that genetic resources should be shared by all humanity. The sharing and openness of crop resources are crucial for the sustainable development of global agriculture and ecological balance, serving as a necessary condition for safeguarding public interests. A key issue that the governance of biotechnology patents urgently needs to address is how to reasonably allocate benefits and risks among traditional communities, researchers, research investors, and the public. This is also essential for constructing a new scientific ethics framework and regulating emerging technologies. However, China’s policy responses in this area are still insufficient. To mitigate the negative effects stemming from the exclusivity of patents, it is imperative to reassess and reconstruct the framework of relevant systems. Firstly, we should adhere to the principle of moral utility, emphasizing the public nature of scientific research and its social responsibilities, while carefully considering the “harmful” nature of inventions to social morality. Secondly, implementing a mandatory disclosure system for biological genetic resources is a crucial step towards achieving transparency and fairness, with “applicants truthfully disclosing the actual origin of crop genes based on the principle of good faith” elevated to a mandatory norm. Lastly, the open licensing of fundamental patented technologies can draw inspiration from the experience of open-source software, encouraging more researchers to participate in the exploration of crop resources through the open sharing of research tools, thereby facilitating broader scientific collaboration and the transformation of research outcomes.

[Purpose/Significance] In recent years, large language models (LLMs) have achieved revolutionary breakthroughs in semantic understanding and generation capabilities through massive text pre-training. This has injected brand-new impetus into the field of knowledge engineering. As a structured knowledge carrier, the knowledge graph has unique advantages in integrating heterogeneous data from multiple sources and constructing an industrial knowledge system. In the context of a paradigm shift in the field of knowledge engineering driven by the emergence of open-source LLMs such as DeepSeek, this study proposes a cost-effective method for constructing domain knowledge graphs based on DeepSeek. We aim to address the limitations of traditional domain knowledge graphs, such as high dependence on expert rules, the high cost of manual annotation, and inefficient processing of multi-source data. [Method/Process] We proposed the semantic understanding-enhanced, cue-engineered domain knowledge extraction technology system, constructed on the methodological framework of manually constructing ontology modelling. In order to process the acquired data, the ETL\MinerU and other tools were used, and the DeepSeek-R1application programming interface was then invoked for intelligent extraction. The ontology model was designed based on domain cognitive features and the multi-source heterogeneous data fusion method was used to achieve the unified characterization of the data structure. Furthermore, the DeepSeek and knowledge extraction were combined. Our system provides a cost-effective reusable technical paradigm for constructing domain knowledge graphs, as well as efficient knowledge extraction, leveraging the advanced powerful textual reasoning ability of the DeepSeek model. [Results/Conclusions] In this study, we take the construction of a domain knowledge map of the entire pig industrial chain as an empirical object. We define the structure of the industrial chain, identify 21 types of core entities and describe their attribute relationships. We achieve the knowledge modelling of the pig industry with a focus on smart farming. The methodology developed in this research was also employed to process and extract knowledge from online and offline resource data. Preliminary experiments demonstrate that DeepSeek-R1 exhibits an F1 value of 0.92 when recognizing the attributes of 161 diseases and 11 types of entities in pig disease control scenarios under zero-sample learning conditions. These experiments also ascertain the reusability of the methodology for other links in the chain. Concurrently, the constructed knowledge map of the entire industrial chain of pigs will be utilized for the design and validation of intelligent application scenarios, with the objective of promoting the intelligent information processing in the pig industry. This study proposes a synergistic paradigm for constructing domain knowledge graphs using DeepSeek, a method that combines deep learning with manual calibration for efficient knowledge extraction and ensure accuracy. This approach ensures the efficiency of knowledge extraction and verifies the knowledge extraction potential of LLMs in vertical domains. The study's findings contribute to the extant literature and offer a practical reference for the promotion of DeepSeek-enabled cost-effective construction of knowledge graphs.

【Objective】Based on whole genome identification and analysis of soybean LOX gene family members, to understand the taxonomic evolutionary relationships of each member, to study the expression specificity of each gene member in different tissues and their response to abiotic stress, which provided a theoretical basis for further research on the molecular characteristics, evolutionary process, and function of the LOX gene family. 【Method】Based on the LOX protein sequences of rice and Arabidopsis species in Ensembl database, BLASTP alignment of homologous LOX protein sequences in soybean whole genome database was performed, and MEGA X software was used to construct a phylogenetic tree; Using website MEME for protein conserved motif analysis; Using online software GSDS 2.0 to analyze gene structure; Using TBtools for chromosome localization drawing; Analyze soybean LOX family replication genes using McscanX; Using the PlantCARE website to predict the promoter elements of soybean LOX gene family; Draw gene expression heatmaps of soybean under different tissues and abiotic stress using TBtools, and develop molecular markers for the excellent allele variant GmLOX15A1^-G/A significantly correlated with 100-seed weight.【Result】A total of 43 LOX genes were identified in soybean, unevenly distributed on 13 chromosomes. Collinearity analysis indicates that the GmLOX gene has undergone extensive replication during the evolutionary process. Meanwhile, 39 different types of cis regulatory elements were detected in the LOX gene promoter, indicating that they may be involved in different pathways such as growth and development, light response, stress response, and hormone induction. Expression pattern analysis revealed that the LOX gene has different levels of expression in different tissues of soybean, indicating that members of this family have tissue and spatiotemporal expression specificity. Under drought stress conditions, the GmLOX gene was significantly differentially expressed in soybean roots and leaves (P<0.05). Among them, GmLOX3A3, GmLOX7A1, GmLOX20B1, GmLOX13A1, and GmLOX20A2 were significantly upregulated or downregulated in roots and leaves, suggesting that the GmLOX gene may play an important role in response to stress. At the same time, it was found that GmLOX15A1 is highly expressed in grain tissue and there is an excellent G/A allele variation in the seventh exon of the gene coding region. Molecular markers were developed for this variant site, and the correlation between different haplotypes of GmLOX15A1 and 100-seed weight was analyzed using 1 200 soybean germplasm resources from different ecological regions over a period of 2 years. The results showed that compared to the GmLOX15A1^-A genotype, the average 100-seed weight of soybean germplasm carrying the GmLOX15A1^-G allele gene increased by 2.33 g (P<0.001). 【Conclusion】A total of 43 members of the LOX family were identified in soybeans, which can be divided into 3 subfamilies. The promoter region of the GmLOX gene contains a large number of cis acting elements that respond to hormones and stress, playing different roles in drought stress response. Among them, GmLOX15A1 is highly expressed in grain tissue and there is an excellent G/A allele variation in the seventh exon of the coding region of this gene. Compared with the GmLOX15A1^-A genotype, the average 100-seed weight of soybean germplasm carrying the GmLOX15A1^-G allele gene is significantly increased by 2.33 g. This locus can be used as an excellent haplotype for genetic improvement of soybean grain size.

【Objective】The GRAS family constitutes a unique class of plant-specific transcription factors that play a pivotal role in plant development and stress response. To elucidate the function of GRAS family genes in wheat heat tolerance，which can provide genetic resources and theoretical foundation for wheat heat-resistant breeding.【Method】A potential heat stress-responsive transcription factor gene, TaGRAS34-5A, was identified through transcriptome analysis of TAM107 and Chinese spring wheat seedlings under high-temperature conditions. Subsequently, a bioinformatics analysis was performed on TaGRAS34-5A, and a phylogenetic tree was constructed to elucidate its molecular characteristics. The expression pattern of TaGRAS34-5A under various stresses, including high temperature, abscisic acid (ABA), ethylene (ETH), and salicylic acid (SA) treatments, were examined using real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) method. The subcellular localization of the TaGRAS34-5A protein was determined using wheat protoplast transient expression technique. Furthermore, the heat tolerance function of TaGRAS34-5A was validated using the heterologous expression system of Saccharomyces cerevisiae and the BSMV:VIGS (Barley stripe mosaic virus: Virus-Induced Gene Silencing) silencing technique. potential interacting proteins of TaGRAS34-5A were screened using yeast two-hybrid technology, and the heat tolerance function was verified, providing preliminary insights into its heat tolerance mechanism.【Result】TaGRAS34-5A, equipped with a characteristic GRAS domain and belongs to the GRAS transcription factor family, is localized to both the cell nucleus and cytoplasm. Bioinformatics analysis indicates that the TaGRAS34-5A promoter contains a large number of hormone response elements and light response elements, and it is most closely related to TaSCL14, OsGRAS23, and AtSCL14 in terms of phylogenetic relationships, suggesting its potential function in responding to oxidative stress. Its expression is upregulated under high-temperature, ethylene (ETH), abscisic acid (ABA), and salicylic acid (SA) treatments, peaking at 4, 6, 0.5, and 12 hours post-treatment, respectively, with the most significant induction observed under heat stress and SA. Functional assays in yeast demonstrated that heterologous expression of TaGRAS34-5A enhances the heat tolerance of the yeast. The results of BSMV:VIGS transient silencing experiment showed that after the 42 ℃ high-temperature treatment, TaGRAS34-5A silenced plants exhibited decreased chlorophyll content, reduced POD enzyme activity, increased cellular peroxidation, and decreased heat tolerance compared to the control. Preliminary studies on the heat tolerance mechanism suggest that TaGRAS34-5A exhibits strong transcriptional self-activation activity.it may modulate wheat heat tolerance by interacting with proteins such as the bZIP family transcription factor HBP-1b and the E3 ubiquitin ligase hel2, thereby regulating cellular redox homeostasis and detoxification processes, positively influencing the heat tolerance of wheat.【Conclusion】TaGRAS34-5A is induced by heat, ABA, ETH, and SA, and its encoded protein is located in the nucleus and cytoplasm. It exhibits transcriptional activation activity. Heterologous overexpression of TaGRAS34-5A enhances the heat tolerance of Saccharomyces cerevisiae. Silencing TaGRAS34-5A in wheat plants increases cellular peroxidation, decreases chlorophyll content, and reduces heat tolerance. TaGRAS34-5A may regulate the heat tolerance of wheat by modulating cellular redox state and detoxification processes.

The article systematically reviewed the research status of Chinese Clematis plants in germplasm resource investigation, cultivation and reproduction, pharmacology and garden applications, analyzed the regional representative distribution of Clematis germplasm resources due to different climatic conditions, and discussed the relationship between Clematis breeding and eco-geographical response. It was concluded that temperature control, light, appropriate amount of plant growth regulator and good culture medium were effective means to improve the breeding technology of Clematis, and the medicinal chemical composition, efficacy and landscaping forms of different species Clematis from the aspect of resource value utilization were further analyzed. It was suggested that the resources of Clematis with little development potential should be protected, and cross-breeding and utilization of the germplasm with good performance in the existing Clematis resources should be done.

The entry of heavy metals into soil may cause degradation of soil quality and lead to changes in the ecological characteristics of soil microorganisms. The impacts of heavy metal types and concentrations on microbial respiratory rate, biomass, biomass carbon, diversity, community structure, and functional genes in soil were summarized. Additionally, research in the field of microbial molecular ecology of heavy metal-contaminated soil was prospected, aiming to provide a reference for studying the effect of heavy metal pollution on soil microbial characteristics.

The planting performance of Zhuliangyou 5298 was combined in Anqing City, Anhui Province area, and its high yield cultivation techniques were summarized. From 2022 to 2024, this variety was planted in 12 demonstration sites, including Wangjiang County in Anqing City, with an upright and upward curved plant shape; the growth period was 130-136 days, with an average seed setting rate of 85.77%, a thousand grain weight of 24.1 g, and a yield of 8 280-9 000 kg/hm²; good polished rice yield and excellent rice quality; strong anti lodging ability. Its high yield cultivation techniques include early sowing (sowing and seedling cultivation from late April to mid May), timely transplanting, cultivating strong seedlings, keeping the soil of the seedling field moist, applying “weaning fertilizer” (urea 60-75 kg/hm²) according to the growth of the seedlings, and timely prevention and control of seedling diseases and pests such as rice thrips and bakanae disease; select high speed rice transplanter operation based on seedling quality, planting time, etc., inspect and debug the transplanting machinery according to the settings, determine the planting distance, seedling amount, and depth; timely weed control (spraying pesticides such as butachlor for soil sealing, spraying pesticides such as butazone for stem and leaf control during the seedling stage, and spraying pesticides such as 30% propiconazole for sealing throughout the field after the seedlings); heavy application of base fertilizer (45% compound fertilizer 450-600 kg/hm²), early application of tillering fertilizer (high nitrogen and high potassium compound fertilizer 225-300 kg/hm²), and supplementary application of ear fertilizer (potassium chloride 112.5-150.0 kg/hm²); shallow water seedling planting, deep water live planting, alternating dry and wet conditions to promote tillering; appropriate pesticides should be used during the growth period of the field to prevent and control diseases and pests such as rice blast and sheath blight. This article provides a reference for further promotion and planting of this variety in similar regions.

The research aims to study the status of molybdenum (Mo) in soils of China, optimize the application technology of molybdenum fertilizer, improving the absorption and utilization efficiency of Mo by crops, and promoting crop growth and yield. The content of total Mo and available Mo in soils of China was clarified through collecting data, the supply of soil Mo was evaluated, and the effects of different molybdenum fertilizer application rates and methods on crop growth and yield were studied. The range of total Mo content in soils of China was 0.1-6 mg/kg, with an average of 1.7 mg/kg, slightly lower than the world average; the content of available Mo in soil was affected by parent material of soil formation, soil pH value, etc., and the available Mo content was low in northern loess parent material soil and acidic soil in the south; the application of molybdenum fertilizer could significantly improve the yield and quality of crops such as soybeans, and the effects of seed treatment and foliar spraying were significant. The content of Mo in soils of China is insufficient, especially in northern loess parent material soil and acidic soil in the south, and molybdenum fertilizer should be applied to improve the availability of soil Mo; the rational application of molybdenum fertilizer is of great importance for improving crop yield and quality, especially in areas with low Mo content; the application methods of molybdenum fertilizer are diverse, and seed treatment and foliar spraying are effective application methods that can improve the absorption and utilization of Mo by crops.

A comparative analysis was conducted between agricultural soil survey data from the 1980s and over 20 000 soil pH samples from the soil testing and formula fertilization project (2010s) in the study area. The trends in soil pH variation, causes of soil acidification, primary control technologies, and appropriate soil improvement measures were explored. The results showed that the average value of soil pH in the study area showed a decreasing trend from 1980s to 2010s, the proportion of neutral soil decreased by 13.1%, the proportion of acidic soil increased by 14.4%, and the proportion of soil area decreased by 1 unit of soil pH accounted for 24.48%. The analysis showed that the accelerated soil acidification was caused by many factors such as the application of soil parent material and chemical fertilizer, land use mode, acid rain, and the reduction of the application amount of alkaline substances such as plant ash. Based on the causes of soil acidification, the current technology of soil acidification resistance and control was discussed, including alkaline neutralization technology, organic fertilizer replacing fertilizer technology, agricultural engineering measures to reduce and control stains, and green fertilizer + biological fertilizer improvement technology.The soil improvement measures suitable for the actual situation of the study area were put forward, including adding alkaline substances such as lime, organic ash and residue and increasing organic fertilizer.

Jasminum species are often deciduous or evergreen small trees, upright or climbing shrubs, widely used in landscaping, medicinal and other fields. On the basis of relevant literature, the resource research progress on the geographical distribution and genetic diversity, ecological and biological characteristics, chemical composition, reproductive technology, and utilization value of Jasminum species were reviewed. In terms of geographical distribution and genetic diversity,there are about 47 species of Jasminum species, with a wide variety of species, widespread distribution, and rich genetic diversity. In terms of ecology and biological characteristics, the flowering period of this species, and the flowers have a strong aroma; the fruits and seeds are generally black spherical, and the plants are sensitive to changes in the external environment. In terms of chemical composition, different parts of this species contain physiologically active substances such as salicylic acid and caffeine, as well as volatile components such as benzyl acetate and linalool, which have broad development and utilization value. In terms of reproductive technology, its plant setting rate is not high, the germination rate of seeds is low, and the main propagation methods are asexual propagation such as cutting and tissue culture. In terms of utilization value, the Jasminum species have medicinal properties such as delaying aging, antibacterial and anti-inflammatory effects; can be applied to wall, balcony, and window greening, with certain ornamental value; in addition, it also has high economic value and cultural connotations.This article provides references for the development of industries related to plants in Jasminum species.

In order to explore the stress of water environment changes on cultured fish in the development of intensive aquaculture, the effects of environmental factors such as temperature, pH, dissolved oxygen, ammonia nitrogen and heavy metals on fish body were studied. From the five aspects of growth performance, blood biochemistry, digestive enzyme activity, antioxidant capacity and immunity, the related mechanism of environmental stress factors affecting fish health was reviewed, and the importance of environmental stress factor research was revealed. It was suggested that comprehensive methods such as field investigation, long-term monitoring and calculation models should be more widely used in the future to comprehensively explore and evaluate the coping strategies and health status of fish in the actual environment, and to explore the molecular mechanism of environmental stress factors on fish health. Future experiments need to study the whole process of fish growth in different periods, which is helpful to predict the impact of future environmental changes on fish community structure and ecosystem function. By studying the adverse effects of environmental stress factors on fish health, the occurrence of stress can be avoided or weakened, which provides a reference for ensuring efficient and healthy aquaculture of fish.

Fusarium oxysporum (Fo) is a soil-borne fungal disease that causes crop wilting, which is a serious constraint to the sustainable development of agriculture. Effectors are key factors in pathogen-plant interactions, and their mechanism of action can be analyzed to provide effective strategies for controlling plant diseases. When Fo infects crops, it secretes a protein in the xylem of vascular bundles, which is called Secreted In Xylem effector protein (SIX). In order to study the role of SIX effectors between Fo and plants, the structural characteristics of SIX on the Fo genome were analyzed. The functional attributes of SIX in identifying different formae speciales and physiological races of Fo, influencing the pathogenicity of Fo, and affecting the immune response of plants were summarized. It was pointed out that SIX had toxic effects on plants and could interact with plants in an incompatible manner to influence the invasion of pathogens. The results of this study reveal the effects of SIX on plants in different aspects and provide theoretical support for the study of the molecular mechanism of plant disease resistance and the selection and breeding of disease-resistant varieties.

Cold stress directly affects plant growth and development, and under extreme conditions, it may result in infertility or even lead to plant mortality. To investigate the molecular mechanisms by which plants respond to cold stress, this review synthesizes the diverse effects of cold stress on plants. It discusses the impact of cold stress on plant plasma membranes, the ICE-CBF-COR signaling pathway, plant hormones, and cellular metabolism. Additionally, recent advancements in understanding the mechanisms underlying plant cold tolerance are examined and discussed. This review aims to provide a foundation for the practical application of these findings in the genetic improvement of crops. Based on the above, the paper suggests that a multidisciplinary approach, incorporating genetic engineering, genetics, biochemistry, molecular biology, and bioinformatics should be employed to further explore the molecular mechanisms behind plant cold tolerance. Furthermore, potential future research directions in this field are proposed.

Peppermint is an herb with a long history in China, and China is also one of the largest peppermint growing countries in the world. Peppermint contains volatile oil and flavonoids, of which menthol is the key component, which has antibacterial and antioxidant effects. Menthol has attracted attention due to its cool aroma and pharmacological effects. This paper reviewed the research progress of menthol detection, extraction and application, including its physical and chemical properties and pharmacological effects. The detection techniques such as gas chromatography, high performance liquid chromatography and gas chromatography-mass spectrometry, as well as the extraction techniques such as CO₂ supercritical extraction, organic solvent extraction and microwave-assisted extraction were introduced. The article also summarized the application of menthol in medicine, food, cosmetics and other fields, and prospects its future development. In depth study of menthol is of great significance to the implementation of national agricultural, rural and farmer policies and rural revitalization strategies.

The strip intercropping model of soybean and corn can fully utilize the edge row advantage and reasonably coordinate the needs of soybean and corn plants for light, fertilizer, and water. The practice of strip intercropping of soybean and corn in Northern Anhui Province was combined, and its high yield cultivation techniques and promotion effects were summarized. High yield cultivation techniques include selecting suitable soybean and corn varieties; reduce plant spacing, ensure density, and choosing soybean corn planting model of 4:2 or 6:4; adopt fungicides, insecticides, etc. for seed coating treatment, and adhere to the “four suitable” sowing methods of suitable period, suitable soil moisture, suitable depth, and suitable formula; applying sufficient basal fertilizer and applying topdressing at the appropriate time; timely check and supplement seedlings, adopting the weed control method of “closed weeding+spraying on stems and leaves after seedlings”, and paying attention to isolation measures; adopting integrated agricultural, physical, biological, and chemical control techniques for disease and pest prevention and control; timely chemical control to prevent plant lodging; after soybeans and corn mature, appropriate machinery is used for harvesting. The research area was promoted the applied this model from 2022 to 2024, achieving the goal of “basically no reduction in corn production and one additional season of beans”. This article provides a reference for promoting the strip intercropping model of soybean and corn in Northern Anhui Province and related areas.

[Purpose/Significance] The spread of misinformation on social media has caused serious harm and attracted attention from various disciplines. This study aims to systematically examine the factors associated with the propagation of misinformation. It contributes to the existing literature by providing an integrated model of the factors influencing misinformation propagation, which is crucial for understanding and mitigating the spread of misinformation. [Method/Process] The search strategy, developed with the help of librarians, was searched in six Chinese and foreign databases. Four researchers coded the information back-to-back to ensure the accuracy of the analysis. Our literature screening criteria were rigorous to ensure that only high quality and relevant research was included. A total of 108 empirical studies related to misinformation propagation were included. The factors were summarized and sorted from multiple perspectives such as disciplinary field, theoretical foundation, research methods and different roles. [Results/Conclusions] Research on this topic has grown rapidly in recent years. Scholars from a variety of disciplines have used survey and experimental methods to study misinformation in the areas of politics and health. Pedictors of misinformation propagation are mainly studed from the perspective of users and information, including objective characteristics of information, perceptual characteristics of information, as well as individual characteristics, cognitive characteristics, and perceptual characteristics of users. The results show that individual characteristics play a critical role in shaping users' intention and behavior to propagate misinformation. Individual characteristics are the most frequently studied factors, while information and situational characteristics have received less attention. Psychological and behavioral variables, including users' cognitive, emotional, and behavioral responses play key mediating roles in this process. In addition, the types of information, individual attributes, cognitive characteristics, social interactions, personal knowledge, behavior and emotions play moderating roles. This study constructs an integrated model of the influencing factors for misinformation propagation, which can provide direction for targeted interventions and algorithm design to mitigate the spread of misinformation. The study also identified some limitations of current studies, including an excessive focus on the political and health issues, a lack of attention to how information characteristics, intervention factors, and platform characteristics play a role, and the relative simplicity of the research methods. Future studies should focus on misinformation propagation in other scenarios, explore more information characteristics suitable for algorithmic intervention, examine the differences in misinformation propagation on different platforms, and use mixed research methods to reach more credible conclusions. This study provides directions and goals for multi-agent collaborative misinformation management.

Through comparative experiments in different breeding periods of crayfish from April to October, 34 quality factors such as conventional nutritional components, texture properties, amino acid composition and content of 25-30 g crayfish meat were analyzed and compared. The results showed that 32 quality factors of crayfish in different breeding periods were significantly different (P<0.05, the same below). The conventional nutritional components and texture properties of crayfish increased first and then decreased, and those of shrimp from June to August were significantly higher than those in other months. The total amino acid (TAA) was 16.57%-19.72%, the highest was in April, followed by July, and the lowest was in October, showing an overall downward trend. The ratio of essential amino acids (EAA) to TAA was 32.63%-36.58%, and the ratio of EAA to non-essential amino acids (NEAA) was 59.67%-73.51%, which was the highest in shrimp from September to October, showing an upward trend. The essential amino acid index (EAAI) was 68.34-90.14, with the highest in April and the lowest in August, showing a downward trend. The correlation analysis between 32 quality factors and meteorological factors in breeding period showed that 23 quality factors were significantly correlated with meteorological factors in breeding period, among which the most quality factors were significantly correlated with the number of cloudy days, sunshine hours and temperature suitability index. They were mostly positively correlated with the number of cloudy days, mostly negatively correlated with the number of sunshine hours, and positively correlated with the temperature suitability index. The optimum temperature of the temperature suitability index related to the conventional nutrients were 22℃, the texture properties were 24-26℃, and the amino acids were 20℃. Therefore, under the appropriate temperature, the appropriate shade was conducive to improving the quality of crayfish. Analyzing the correlation between meteorological factors and quality factors in different periods, the results showed that the early stage of breeding was the critical impact period of meteorological factors on conventional nutrients and amino acids, and the middle and late stages of breeding was the critical impact period of meteorological factors on texture properties.

【Objective】 This study aims to discover new quantitative trait loci (QTLs) for resistance to the brown planthopper (BPH) in rice by utilizing a genetic population and to assess the pyramiding effects of these QTLs, thereby providing crucial genetic materials and resources for breeding insect-resistant rice. 【Method】 A recombinant inbred line (RIL) population derived from a cross between susceptible NPB and resistant Jiafuzhan was used, combined with seedling stage resistance evaluation and extreme mixed pool sequencing (BSA-seq) to map BPH resistance QTLs. Further, fine mapping, candidate gene identification, pyramiding effect analysis, and mixed-pool transcriptome sequencing (BSR-seq) were employed to elucidate the physiological and molecular mechanisms mediated by these QTLs. 【Result】 BSA-seq identified two major resistance QTLs on chromosomes 1 (30-32 Mb) and 4 (5-7 Mb), named QBPH1 and QBPH4, respectively. Interval-linked molecular markers confirmed the authenticity of these QTLs. QBPH4 coincides with previously cloned BPH3 and BPH15, while QBPH1 represents a newly discovered QTL. High-density markers and recombinant analysis further narrowed the QBPH1 region to 30.61-30.65 Mb. This analysis identified Os01g53294 and Os01g53330 as reliable candidate genes, which code for a respiratory burst oxidase protein B and an anthocyanin 5,3-O-glucosyltransferase, respectively. In evaluations of seedling resistance, brown planthopper honeydew area and weight, insect weight gain, lethality, and preference, comparisons between QBPH1 and QBPH4 single genes and their polymerized lines revealed no significant enhancement in resistance. Both QBPH1 and QBPH4 mediated antibiosis and antixenosis mechanisms with varying effects. BSR-seq analysis highlighted significant enrichment of differentially expressed genes (DEGs) involved in transcriptional regulation, protein phosphorylation, and redox processes among different QBPH1 alleles. Additionally, genes associated with jasmonic acid (JA) synthesis and signaling pathways were significantly upregulated in resistant materials, confirmed by RT-qPCR experiments. 【Conclusion】 A novel BPH resistance QTL, QBPH1, was successfully identified on chromosome 1 of rice. QBPH1 mediated both antibiosis and avoidance against BPH, though its aggregation effect with another QTL was not significant. QBPH1 may mediate defense mechanisms against BPH through involvement in the JA pathway. Based on this, Os01g53294 and Os01g53330 have been validated as reliable candidate genes for QBPH1.

【Objective】Taikemai33, derived from a cross between Zhengmai366 and Huaiyin9908, is a new released wheat cultivar with high quality, high yield, and excellent disease resistance, which has a broad genetic base, and a high potential for application in wheat production. The objective of this study is to dissect the genetic composition of Taikemai33 to provide information for parental selection to use this cultivar to develop more new wheat cultivars. 【Method】Taikemai33 and its pedigree parents including Zhengmai366, Huaiyin9908, Yumai47, PH82-2-2, Yumai13, Yumai 2 hao, Bainong3217, Yanda24, Xiannong39, Fengchan 3 hao and Funo were screened using the 55K wheat SNP chip to dissect the genomic composition of Taikemai33 to evaluate the genetic contributions of each parental line to Taikemai33. 【Result】The similarity coefficient between Taikemai33 and its pedigree parents ranged from 0.72 to 0.93, and the genetic composition of Taikemai33 was highly similar to Zhengmai366, the pedigree mother parent, with a genetic similarity coefficient of 0.93. SNP marker analysis showed that the pedigree parents contributed different proportion to the genome of Taikemai33, with the pedigree mother contributed 66.57%, whereas the pedigree father contributed 33.43%, indicating that Taikemai 33 inherits more genetic materials from the maternal lineage. Furthermore, the pedigree mother contributed 71.0%, 85.0% and 49.4% to subgenome A, B and D of Taikeimai33, whereas those were 29.0%, 15.0% and 50.6% contributed by the pedigree father. For each chromosome, the pedigree mother contributed more on chromosome 1A, 2A, 3A, 4A, 7A, 1B to 7B, 1D and 2D, whereas the pedigree father contributed more on chromosome 5A, 4D, 6D and 7D. The contributions of the pedigree parents on 6A, 3D and 5D were equal. Taikemai33 genotype map showed that the contribution loci of the pedigree mother were distributed in clusters on chromosome 1A, 5A, 7A, 2B, 7B, 2D, with those from the pedigree father were on chromosome 4A, 5A, 6D, 7D. Interestingly, among the polymorphic SNP loci, between Zhenmai366 and Huaiyin9908, Taikemai33 showed 109 loci that were absent in both parents, distributing on 19 chromosomes except 1A and 6A. Chromosome 4A, 2B, 6B and 7D of Taikemai33 confer most of the polymorphic SNPs in clusters with cluster number of 10, 9, 11, and 9. 【Conclusion】We constructed the genotype map and dissected the genetic composition of Taikemai33, determined the loci contributed by the pedigree parents and identified that Taikemai33 inherited more genetic materials from the pedigree mother and conferring some specific loci different with the pedigree parents.

【Objective】Polymerizing soybean high oil genotypes aims at breeding varieties with higher oil content to improve economic efficiency and nutritional value. It is of great significance to increase agricultural output, reduce processing costs and meet global demand for vegetable oil growth.【Method】Glyma.18G027100 C2 gene family was identified by bioinformatic analysis method at the whole genome level. A total of 66 soybean C2 gene family members were identified, named GmC2-01.1-GmC2-20.2 according to chromosome position. Tissue pattern analysis revealed that 7 genes were highly expressed in grains among 66 C2 family genes (GmC2-03.6, GmC2-02.7, GmC2-07.2, GmC2-18.1, GmC2-18.4, GmC2-19.1 and GmC2-20.2). In order to analyze the effect sites of these genes on soybean oil content, SNP sites in the coding regions of these genes were obtained from SFGB database. Correlation analysis of oil content in two years showed that GmC2-18.1 has SNP loci that significantly affect oil content. The genetic diversity of GmC2-18.1 coding region was analyzed by 12 extreme materials. There was a G/A mutation at 2 038 273 bp in coding region of Wm82.a2.v1 version, which regulated seed oil content. It was preliminarily speculated that this gene played a role in seed development or nutrient accumulation. Then, SNP/InDel molecular markers were developed for GmC2-18.1^-G/A gene combined with InDel natural allelic variation site 225 bp upstream of the start codon of GmSWEET39, T/C natural allelic variation site at 8 381 058 bp in coding region of GmST1, A/C natural allelic variation site at the third exon of 41 854 422 bp in coding region of GmMFT. 1 200 soybean germplasm resources from three ecological regions in China were identified by markers in 2 years.【Result】Analysis of variance showed that GmC2-18.1^-G, GmSWEET39^-Deletion, GmST1^-T and GmMFT^-A significantly increased oil content by 1.72, 1.95, 1.58 and 2.06 percentage points (P<0.01). The results showed that the average oil content of soybean seeds carrying GmC2-18.1^-G, GmSWEET39^-Deletion, GmST1^-T and GmMFT^-A high-oil allele type (PFAT-1) was 22.89%, which increased by about 4.5% compared with that carrying GmC2-18.1^-A, GmSWEET39^-Insertion, GmST1^-C and GmMFT^-C low-oil allele type (PFAT-14). 5 percentage points, the contribution rate to oil content is about 21.69%. 【Conclusion】Based on the markers developed above, 115 PFAT-1 high oil alleles were screened.

In this paper, the research status of edible dock was briefly described from the aspects of nutritional value, safety, extraction technology of protein and active substances, food and health products, feeding, soil improvement and cultivation technology, etc. It was analyzed that the values of nutrition, health care, food use, feeding use and soil improvement of edible dock were high, but people’s recognition of leaf-eating grass was low, the related basic and applied technology research was still in the initial stage, and the development of the industry was slow. Three suggestions were put forward to promote the research and industrial development of edible dock, to improve people’s recognition of edible dock through propaganda and production of high-value products of edible dock; accelerate the research on the introduction and cultivation of edible dock, and promote intensive and large-scale planting; strengthen the research on the basic and applied technology of edible dock in animal husbandry and soil improvement.

Hyperspectral remote sensing is an important technology for crop research. In order to promote the healthy development and basic research of crop growth cycle, improve the application level of remote sensing technology in crop research, this paper summarizes the analysis algorithms of hyperspectral in crop research, focus on the research progress of hyperspectral in crop growth, crop information monitoring, and crop information extraction. This paper systematically reviews the studies on leaf area index, biomass estimation, nitrogen monitoring, chlorophyll monitoring, pest and disease monitoring, heavy metal monitoring, crop carbon to nitrogen ratio, and water content, which still need to be further developed. Analysis shows that the combination of hyperspectral technology and artificial intelligence has achieved initial results in crop research which is still difficult to meet the intelligent needs of modern agriculture. If the new generation of information technology and large models are used to empower, enormous research potential will be unleashed.