Phenotypic Diversity Analysis and Comprehensive Evaluation of Wild Germplasm Resources of Gentiana rigescens

SHEN Tao,WANG Yuan-zhong

PDF(1167 KB)
PDF(1167 KB)
Journal of Plant Genetic Resources ›› 2023, Vol. 24 ›› Issue (1) : 181-193. DOI: 10.13430/j.cnki.jpgr.20220822005
Research Articles

Phenotypic Diversity Analysis and Comprehensive Evaluation of Wild Germplasm Resources of Gentiana rigescens

  • SHEN Tao1, WANG Yuan-zhong2
Author information +
History +

Abstract

The purpose of this study was to explore the phenotypic diversity of Gentiana rigescens Franch. ex Hemsl and establish a comprehensive evaluation method for excellent provenances of the species. The phenotypic datasets of 19 traits in 477 individuals from Yunnan province were collected. The Shannon-Wiener diversity index, principal component analysis, membership function, and partial least square were used to evaluate the provenances of G. rigescens Franch. The value of Shannon-Wiener diversity index(H')of root, stem, and leaf traits of 477 individuals ranged from 1.59 to 2.06. At the several traits such as root diameter(H'=2.00), stem diameter(H'=2.00), root/biomass ratio(H'=2.02) and root water content(H'=2.06), the highest H' values were observed. The dry weight of aerial part of plants showed the lowest H' value(H'=1.59). The plant traits of G. rigescens Franch. which was from different geographical regions were significant differences(P<0.05). Notably, the root yield traits value of G. rigescens Franch. from southern Yunnan was significantly higher than those in other distribution areas(P<0.05). Based on the comprehensive evaluation value(D value)of phenotypes, the D value of 477 G. rigescens Franch. ranged from 0.50 to 2.45. The individuals collected from western and northwestern Yunnan(D=1.17±0.03)and southern Yunnan(D=1.19±0.37)had higher comprehensive evaluation value. Meanwhile, the D value of the individuals collected from central Yunnan(D=1.00±0.30)and southeastern Yunnan(D=1.00±0.32) were lower. The Yunxian population had the highest comprehensive evaluation value of plant(D=1.24±0.14), and small differences in traits among individuals within the population(CV=11.02%). It suggested that individuals of Yunxian population were excellent provenances. Through the analysis of PLS-R and PLS-DA, it was found that root fresh weight, root dry weight, dry weight of plant, branch number, fiber number, dry weight of aerial part, stem diameter and leaf length(VIP>1.00)were the key traits for the discrimination of good and bad provenances. And the environmental factors such as soil properties, precipitation during plant growing period and average temperature in the driest season were closely related to the high yield traits of G. rigescens Franch.(VIP>1.00). The higher content of sandy and the lower content of clay in soil, drought and high temperature might lead to reduce of root fresh weight, dry weight and root number. The higher precipitation in April, May and November was observed with positive effects at root-relates traits.

Key words

Gentiana rigescens / phenotypic diversity / membership function / comprehensive evaluation / environment factors

Cite this article

Download Citations
SHEN Tao,WANG Yuan-zhong. Phenotypic Diversity Analysis and Comprehensive Evaluation of Wild Germplasm Resources of Gentiana rigescens. Journal of Plant Genetic Resources. 2023, 24(1): 181-193 https://doi.org/10.13430/j.cnki.jpgr.20220822005

References

[1]国家药典委员会. 中华人民共和国药典一部. 北京: 中国医药科技出版社, 2020: 99-100Chinese Pharmacopoeia Commission. Chinese pharmacopoeia Part I. Beijing: China Medical Science Press, 2020: 99-100
[2]李智敏, 刘莉, 李晚谊, 张金渝, 金航. 滇龙胆的药用资源研究与开发进展. 云南大学学报: 自然科学版, 2009, 31 (S1): 485-487Li Z M, Liu L, Li W Y, Zhang J Y, Jin H. Progress on research and development of Gentiana rigescens as a raw material. Journal of Yunnan University: Natural Sciences, 2009, 31 (S1): 485-487
[3]杨利民. 中药材生态种植理论与技术前沿. 吉林农业大学学报, 2020, 42 (4): 355-363Yang L M. Theory and technology frontiers of ecological planting of Chinese medicinal materials. Journal of Jilin Agricultural University, 2020, 42 (4): 355-363
[4]王继永, 郑司浩, 曾燕, 刘美娟, 尚兴朴, 王浩. 中药材种质资源收集保存与评价利用现状. 中国现代中药, 2020, 22 (3): 311-321Wang J Y, Zheng S H, Zeng Y, Liu M J, Shang X P, Wang H. Current situation on collection, preservation, evaluation and utilization of germplasm resources for traditional Chinese medicine. Modern Chinese Medicine, 2020, 22 (3): 311-321
[5]叶清华, 刘林敏, 邱娜娜, 谢倩, 王威, 陈清西. 橄榄 (Canarium album) 种子形态结构与数量性状分析. 植物科学学报, 2019, 37 (4): 413-421Ye Q H, Liu L M, Qiu N N, Xie Q, Wang W, Chen Q X. Seed morphological structure and quantitative trait analysis of Canarium album. Plant Science Journal, 2019, 37 (4): 413-421
[6]刘娜, 成仿云. 紫斑牡丹果实性状变异及其产量分级标准研究. 植物科学学报, 2020, 38 (3): 378-389Liu N, Cheng F Y. Variation and yield grading standards of fruit traits in Paeonia rockii ( S. G. Haw et L. A. Lauener) T. Hong et J. J. Li. Plant Science Journal, 2020, 38 (3): 378-389
[7]谢影, 于海洋, 庞忠义, 张明, 张世凯, 赵曦阳. 东北地区6种槭属植物种子表型多样性分析及优良家系选择. 植物科学学报, 2021, 39 (6): 610-619Xie Y, Yu H Y, Pang Z Y, Zhang M, Zhang S K, Zhao X Y. Phenotypic diversity analysis and superior family selection of seed traits in six Acer species in northeast China. Plant Science Journal, 2021, 39 (6): 610-619
[8] Chung H H, Shi S K, Huang B, Chen J T. Enhanced agronomic traits and medicinal constituents of autotetraploids in Anoectochilus formosanus Hayata, a top-grade medicinal orchid. Molecules, 2017, 22 (11): 1907
[9] Zhang W, Lu B Y, Meng H L, Wei X, Yang Z Q, Yang S C. Phenotypic diversity analysis of the fruit of Amomum tsao-ko Crevost et Lemarie, an important medicinal plant in Yunnan, China. Genetic Resources and Crop Evolution, 2019, 66 (5): 1145-1154
[10]周新华, 曾平生, 武晓玉, 黄宇涛. 华重楼野生种群表型变异研究. 华南农业大学学报, 2021, 42 (1): 116-124Zhou X H, Zeng P S, Wu X Y, Huang Y T. Phenotypic variations in wild populations of Paris polyphylla var. chinensis. Journal of South China Agricultural University, 2021, 42 (1): 116-124
[11] Zhang D Z, Panhwar R B, Liu J J, Gong X W, Liang J B, Liu M X, Lu P, Gao X L, Feng B L. Morphological diversity and correlation analysis of phenotypes and quality traits of proso millet (Panicum miliaceum L.) core collections. Journal of Integrative Agriculture, 2019, 18 (5): 958-969
[12] Wang Z H, Yang P X, Peng H, Li C, Yue C N, Li W J, Jiang X F. Comprehensive evaluation of 47 tea [Camellia sinensis (L.) O. Kuntze] germplasm based on entropy weight method and grey relational degree. Genetic Resources and Crop Evolution, 2021, 68 (8): 3257-3270
[13] Yan C J, Song S H, Wang W B, Wang C L, Li H B, Wang F, Li S Y, Sun X G. Screening diverse soybean genotypes for drought tolerance by membership function value based on multiple traits and drought-tolerant coefficient of yield. BMC Plant Biology, 2020, 20 (1): 1-15
[14] Wu H, Guo J R, Wang C F, Li K L, Zhang X W, Yang Z, Li M T, Wang B S. An effective screening method and a reliable screening trait for salt tolerance of Brassica napus at the germination stage. Frontiers in Plant Science, 2019, 10: 530
[15]王秀秀,邢爱双,杨茹,何守朴,贾银华,潘兆娥,王立如,杜雄明,宋宪亮. 陆地棉种质资源表型性状的综合评价. 中国农业科学, 2022, 55 (6): 1082-1094Wang X X, Xing A S, Yang R, He S P, Jia Y H, Pan Z E, Wang L R, Du X M, Song X L. Comprehensive evaluation of phenotypic characters of nature population in upland cotton. Scientia Agricultura Sinica, 2022, 55 (6): 1082-1094
[16]李洪磊, 王晓敏, 郑福顺, 李国花, 刘珮君, 胡新华, 付金军, 高艳明, 李建设. 基于主成分和隶属函数分析的不同果色番茄品种引种初步评价. 云南大学学报 :自然科学版, 2021, 43 (2): 402-411Li H L, Wang X M, Zheng F S, Li G H, Liu P J, Hu X H, Fu J J, Gao Y M, Li J S. Preliminary evaluation of different fruit color tomato varieties introduction based on principal component and membership function analysis methods. Journal of Yunnan University:Natural Sciences Edition, 2021, 43 (2): 402-411
[17]钱润, 李慧, 华中一, 袁媛, 谢冬梅. 栽培天麻农艺性状的数量分类学研究. 中国中药杂志, 2020, 45 (13): 3085-3090Qian R, Li H, Hua Z Y, Yuan Y, Xie D M. Quantitative taxonomic study on agronomic traits of cultivated Gastrodia elata. China Journal of Chinese Materia Medica, 2020, 45 (13): 3085-3090
[18]宋雪彬, 高康, 黄河, 刘芷兰, 戴思兰, 嵇彧. 中国传统大菊叶片形态的数量化定义与分类. 植物学报, 2021, 56 (1): 10-24Song X B, Gao K, Huang H, Liu Z L, Dai S L, Ji Y. Quantitative definition and classification of leaves in large-flowered Chinese chrysanthemum based on the morphological traits. Bulletin of Botany, 2021, 56 (1): 10-24
[19] Lu C F, Li Y F, Wang J Y, Qu J P, Chen Y, Chen X Y, Huang H, Dai S L. Flower color classification and correlation between color space values with pigments in potted multiflora chrysanthemum. Scientia Horticulturae, 2021, 283: 110082
[20]王元忠, 沈涛, 张金渝. 滇重楼及其近缘种的表型变异与资源评价. 热带作物学报, 2021, 42 (9): 2535-2541Wang Y Z, Shen T, Zhang J Y. Phenotypic variation and resource evaluation of Paris polyphylla var. yunnanensis and its relative species. Chinese Journal of Tropical Crops, 2021, 42 (9): 2535-2541
[21]张金渝, 沈涛, 杨维泽, 张霁, 王元忠, 赵振玲, 张智慧, 金航. 云南道地药材滇龙胆资源调查与评价. 植物遗传资源学报, 2012, 13 (5): 890-895Zhang J Y, Shen T, Yang W Z, Zhang J, Wang Y Z, Zhao Z L, Zhang Z H, Jin H. Investigation and evaluation of Gentiana rigescens resource in Yunnan province. Journal of Plant Genetic Resources, 2012, 13 (5): 890-895
[22]沈涛, 虞泓, 王元忠. 滇龙胆草野生资源的地理分布与生物气候特征. 应用生态学报, 2019, 30 (7): 2291-2300Shen T, Yu H, Wang Y Z. Geographical distribution and bioclimatic characteristics of the wild Gentiana rigescens resources. Chinese Journal of Applied Ecology, 2019, 30 (7): 2291-2300
[23]杨维泽, 金航, 杨美权, 赵振玲, 张智慧, 王元忠, 张金渝. 云南滇龙胆居群表型多样性及其与环境关系研究. 西北植物学报, 2011, 31 (7): 1326-1334Yang W Z, Jin H, Yang M Q, Zhao Z L, Zhang Z H, Wang Y Z, Zhang J Y. Phenotypic diversity and environment relations in Gentiana rigescens of Yunnan. Acta Botanica Boreali-Occidentalia Sinica, 2011, 31 (7): 1326-1334
[24]杨雁, 邵爱娟, 金航, 欧小宏, 陈美兰, 刘大会, 黄璐琦. 云贵高原滇龙胆不同居群形态特征变异研究. 中草药, 2012, 43 (8): 1604-1610Yang Y, Shao A J, Jin H, Ou X H, Chen M L, Liu D H, Huang L Q. Variation of botanical morphologic characteristics between wild and cultivated populations of Gentiana rigescens in Yunnan-Guizhou Plateau. Chinese Traditional and Herbal Drugs, 2012, 43 (8): 1604-1610
[25]杨天梅, 杨美权, 杨绍兵, 许宗亮, 杨维泽, 张金渝, 左应梅. 坚龙胆药材产品质量分级标准研究. 中药材, 2018, 41 (3): 648-651Yang T M, Yang M Q, Yang S B, Xu Z L, Yang W Z, Zhang J Y, Zuo Y M. Study on the quality grading standard of Gentiana rigescens. Journal of Chinese Medicinal Materials. 2018, 41 (3): 648-651
[26] He X J, Augusto L, Goll D S, Ringeval B, Wang Y P, Helfenstein J L, Huang Y Y, Yu K L, Wang Z Q, Yang Y C, Hou E Q. Global patterns and drivers of soil total phosphorus concentration. Earth System Science Data, 2021, 13 (12): 5831-5846
[27]宫彦龙, 雷月, 闫志强, 刘雪薇, 张大双, 吴健强, 朱速松. 不同生态区粳稻资源表型遗传多样性综合评价. 作物杂志, 2020, 36 (5): 71-79Gong Y L, Lei Y, Yan Z Q, Liu X W, Zhang D S, Wu J Q, Zhu S S. Comprehensive evaluation of phenotype genetic diversity in japonica rice germplasm resources in different ecological zones. Crops, 2020, 36 (5): 71-79
[28]王声跃. 云南地理. 昆明: 云南民族出版社, 2002: 65-82Wang S Y. Yunnan dili. Kunming: Yunnan Nationalities Publishing House, 2002: 65-82
[29]郑度. 中国自然地理总论. 北京: 科学出版社, 2015: 54-83Zheng D. General physical geography of China. Beijing: Science Press, 2015: 54-83
[30]杨雁, 金航, 王家金, 陈美兰, 石亚娜, 欧小宏, 刘大会. 大理苍山不同海拔梯度和土壤对野生滇龙胆生长及其成分的影响. 西南农业学报, 2014, 27 (4): 1601-1606Yang Y, Jin H, Wang J J, Chen M L, Shi Y N, Ou X H, Liu D H. Effects of different elevation gradient and soil nutrient on growth and component of wild Gentiana rigescens in Yunnan Dali Cangshan mountain. Southwest China Journal of Agricultural Sciences. 2014, 27 (4): 1601-1606
[31]沈涛, 张金渝, 杨维泽, 杨美权, 赵振玲, 张智慧, 陈秀花, 金航, 王跃华. 滇龙胆主要农艺性状的相关及通径分析. 中国中药杂志, 2011, 36 (12): 1568-1571Shen T, Zhang J Y, Yang W Z, Yang M Q, Zhao Z L, Zhang Z H, Chen X H, Jin H, Wang Y H. Correlation and path analysis on agronomic traits of Gentiana rigescens. China Journal of Chinese Materia Medica, 2011, 36 (12): 1568-1571
[32]陈嘉静, 余华, 徐朝斌, 钟全林. 种源与同质园环境对刨花楠幼苗叶功能性状的影响. 应用与环境生物学报, 2019, 25(3): 648-654Chen J J, Yu H, Xu C B, Zhong Q L. Effects of provenance and common garden environment on leaf functional traits of Machilus pauhoi seedlings. Chinese Journal of Applied & Environmental Biology, 2019, 25(3): 648-654
[33]谭美, 杨志玲, 杨旭, 程小燕. 厚朴野生资源的野外回归植株苗期适应性评价. 林业科学研究, 2019, 32(1): 125-132Tan M, Yang Z L, Yang X, Chen X Y. Adaptability evaluation of wild Houpoёa officinalis seedling in the process of reintroduction. Forest Research, 2019, 32(1): 125-132
[34]苑海静, 成向荣, 虞木奎, 汪阳东, 邰建武, 张春祥. 不同种源麻栎生长性状的地理变异. 应用生态学报, 2021, 32(8): 2791-2799Yuan H J, Cheng X R, Yu M K, Wang Y D, Tai J W, Zhang C X. Geographical variation of growth traits among different Quercus acutissima provenances. Chinese Journal of Applied Ecology, 2021, 32(8): 2791-2799

Funding

Foundation projects: The National Natural Science Foundation of China (32060086);The Young and Middle-aged Academic and Technical Leaders Reserve Talents Program of Yunnan Province(202205AC160088)
Share on Mendeley
PDF(1167 KB)

60

Accesses

0

Citation

Detail

Sections
Recommended

/