Isolation of SFB Genes of 82 Malus Germplasm Resources and Gene Frequency Analysis of Different Ecological Populations

LIU Zhao,GAO Yuan,WANG Kun,FENG Jian-rong,SUN Si-miao,LU Xiang,LI Qing-shan,LI Lian-wen,WANG Da-jiang

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Journal of Plant Genetic Resources ›› 2023, Vol. 24 ›› Issue (1) : 215-225. DOI: 10.13430/j.cnki.jpgr.20220728001
Research Articles

Isolation of SFB Genes of 82 Malus Germplasm Resources and Gene Frequency Analysis of Different Ecological Populations

  • LIU Zhao1,2, GAO Yuan1, WANG Kun1, FENG Jian-rong2, SUN Si-miao1, LU Xiang1,2, LI Qing-shan1,2, LI Lian-wen1, WANG Da-jiang1
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Abstract

In order to clarify the SFB gene diversity in Malus Mill. and the distribution frequency of each sequence haplotype in ecological populations, 82 Malus Mill. germplasm resources including the cultivated apples (i.e. Kuihua) and wild resources (i.e. Malus sieversii(Ledeb.) M. Roem.) were used. The genotyping was conducted using three gene-specific primer combinations. PCR amplification using leaf DNA produced a single SFB gene fragment in all materials. Sanger sequencing of PCR products revealed these fragments belonged to five different SFB genes, corresponding SFB1SFB2SFB3SFB7and SFB9 based on GenBank homology comparison. At the same time, the distribution frequency of SFB gene in different ecological population was analyzed. The frequencies of different haplotypes in populations were different, and the highest frequency of SFB3was 43.90%, followed by SFB1(39.00%). The intraspecific and interspecific frequency of the same SFB gene was also different. In addition, the phylogenetic tree analysis showed that Malus sieversii (Ledeb.) M. Roem. was related to the domestication and origin of Malus turkmenorumJuz. & M. Pop. and apple cultivars.

Key words

Malus Mill. / germplasm resource / SFB gene / clone / frequency

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LIU Zhao,GAO Yuan,WANG Kun,FENG Jian-rong,SUN Si-miao,LU Xiang,LI Qing-shan,LI Lian-wen,WANG Da-jiang. Isolation of SFB Genes of 82 Malus Germplasm Resources and Gene Frequency Analysis of Different Ecological Populations. Journal of Plant Genetic Resources. 2023, 24(1): 215-225 https://doi.org/10.13430/j.cnki.jpgr.20220728001

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Funding

Foundation project: The Agricultural Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences (CAAS-ASTIP-2021-RIP-02)
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