Functional Analysis of a small GTP-binding Protein Gene GhROP4 in Drought Resistance of Cotton（Gossypium hirsutum L.）

HU Zi-yao,ZHANG Lang-rang,HUANG Hui-ying,LEI Jian-feng,MADINA Mulati,DENG Jia-hui,SUN Ling,LIU Min,XU Shi-jia and LI Yue

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Journal of Plant Genetic Resources ›› 2022, Vol. 23 ›› Issue (2) : 614-623. DOI: 10.13430/j.cnki.jpgr.20211031001
Research Articles

Functional Analysis of a small GTP-binding Protein Gene GhROP4 in Drought Resistance of Cotton（Gossypium hirsutum L.）

  • HU Zi-yao1, ZHANG Lang-rang2, HUANG Hui-ying1, LEI Jian-feng2, MADINA Mulati2, DENG Jia-hui1, SUN Ling2, LIU Min1, XU Shi-jia2, LI Yue1
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Abstract

Drought is the primary abiotic factor that affects the yield and quality of cotton fiber. ROP （Rho-realted GTPases from plants）protein is a type of plant-specific Rho small G proteins，and in plants this protein plays a role in responses to the development and various stresses. In this study，real-time quantitative polymerase chain reaction（qRT-PCR）was used to analyze the expression pattern of the GhROP4 gene under drought stress and exogenous abscisic acid（ABA）treatment conditions. Virus-induced gene silencing（VIGS） technology was used to analyze the regulation mode of the GhROP4 gene on drought and the possible regulatory pathways involved. Drought and abscisic acid treatment increased the transcription level of GhROP4 gene as a whole，and silencing GhROP4 gene enhanced the drought resistance of cotton. The water loss rate and relative water content，proline（Pro，Proline）content，and activities of three antioxidant enzymes：catalase（CAT）， superoxide dismutase（SOD），and peroxidase（POD）of the detached leaves of the silent plants after drought stress were significantly higher than those of the negative control. The contents of hydrogen peroxide（H2O2）and malondialdehyde（MDA）were significantly lower than those of the negative control；compared with the negative control，ABA synthesis and pathway gene expression in the leaves of silent plants were significantly up-regulated. Altogether，our results supported that the GhROP4 gene was a negative regulator in drought resistance of cotton through negatively regulating the ABA metabolic pathway

Key words

cotton；drought；GhROP4；virus-induced gene silencing（VIGS）；abscisic acid

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HU Zi-yao,ZHANG Lang-rang,HUANG Hui-ying,LEI Jian-feng,MADINA Mulati,DENG Jia-hui,SUN Ling,LIU Min,XU Shi-jia and LI Yue. Functional Analysis of a small GTP-binding Protein Gene GhROP4 in Drought Resistance of Cotton（Gossypium hirsutum L.）. Journal of Plant Genetic Resources. 2022, 23(2): 614-623 https://doi.org/10.13430/j.cnki.jpgr.20211031001

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National Natural Science Foundation of China（31560534）
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