Detection of Quality-Related Genes in the WheatLandrace in Shanxi Province by KASP Markers

LIU Xia,LEI Meng-lin,WANG Yan-zhen,WANG Yu-nan,HUANG Rui and MU Zhi-xin

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Journal of Plant Genetic Resources ›› 2022, Vol. 23 ›› Issue (3) : 881-894. DOI: 10.13430/j.cnki.jpgr.20210910001
Research Articles

Detection of Quality-Related Genes in the WheatLandrace in Shanxi Province by KASP Markers

  • LIU Xia, LEI Meng-lin, WANG Yan-zhen, WANG Yu-nan, HUANG Rui, MU Zhi-xin
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Abstract

To explore and utilize the genetic resources of good quality traits in wheat landraces,15 quality trait-related genes in 405 wheat landraces of Shanxi province were genotyped using 30 KASP markers. The results showed that the average frequency of the favorable allelic variations Pinb-D1b and Pinb-B2b for grain hardness accounted for 4.94% and 63.46%,respectively. At the Glu-1 locus,the favorable alleles Glu-Ax1(or Glu-Ax2* ),Glu-Bx7OE and Glu-D1d accounted for 93.09%,47.16% and 0.74%,respectively. Elite alleles for flour color including, Psy-A1b,Psy-B1a(or Psy-B1b),Psy-D1a,TaPds-B1b,Zds-A1a,TaLcy-B1b,Lox-B1a,TaPod-A1b,Ppo-A1b and Ppo-D1a accounted for 1.23%,95.80%,87.65%,0.74%,87.16%,96.05%,88.89%,1.73%,97.28% and 20.94%, respectively. The favorable allele frequncy of landraces,which were derived from the late-maturing winter wheat region in central Shanxi and the south mid-maturing winter wheat region,ranged from 0.52% to 97.41% and 0 to 97.51%,respectively. In addition,nine favorable alleles Pinb-B2b/Glu-Ax1(or Glu-Ax2* )/Glu-Bx7OE/Psy-B1a (or Psy-B1b)/Psy-D1a/Zds-A1a/TaLcy-B1b/Lox-B1a/Ppo-A1b were simultanously detected in 89 samples,with a frequency of 21.98%. By conducting marker-assisted identification of the excellent alleles in wheat landraces,this study provided reference for the genetic improvement at quality-related traits in bread wheat.

Key words

wheat landrace;quality;KASP marker;Shanxi province

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LIU Xia,LEI Meng-lin,WANG Yan-zhen,WANG Yu-nan,HUANG Rui and MU Zhi-xin. Detection of Quality-Related Genes in the WheatLandrace in Shanxi Province by KASP Markers. Journal of Plant Genetic Resources. 2022, 23(3): 881-894 https://doi.org/10.13430/j.cnki.jpgr.20210910001

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Funding

Shanxi Province General Youth Fund Project(201801D221313),Shanxi Academy of Agricultural Sciences AgriculturalScience and Technology Innovation Research Project(YCX2020YQ48),Key Research and Development Projects in ShanxiProvince(201903D211003)
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