Yeast Two-Hybrid Screening of the Proteins Interacting withAhMYB44 from Peanut

LIU Yong-hui,SHEN Yi,SHEN Yue and CHEN Zhi-de

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Journal of Plant Genetic Resources ›› 2020, Vol. 21 ›› Issue (1) : 201-207. DOI: 10.13430/j.cnki.jpgr.20191029002
Research Articles

Yeast Two-Hybrid Screening of the Proteins Interacting withAhMYB44 from Peanut

  • LIU Yong-hui1,2, SHEN Yi1, SHEN Yue1, CHEN Zhi-de1
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Abstract

We previously cloned the full-length coding sequence of the gene AhMYB44,which showed a stress-induced expression from peanut drought transcriptome sequencing datasets. In order to investigate the function of AhMYB44,a yeast two-hybrid system was deployed for identifying its interacting proteins. The results showed that the bait vector pGBKT7-AhMYB44 was not toxic and showed no self-activation activity. Sixteen coding sequences were identified by screening of cDNA libraries which were constructed from a drought and high-salt stress of peanut. We further conducted an in-silico analysis of their homologous proteins,such as plant transcriptional regulation,growth and development,signal transduction,biotic and abiotic stress responses. Out of that,the candidate gene(No.46)was annotated with the abiotic stress,and encoded for a calcium-dependent phospholipid binding protein(ANNEXIN). Therefore,we speculated that AhMYB44 maybe participate into regulation of peanut stress response process through intracellular calcium signal. This result might provide a reference for further research on the function and mechanism of AhMYB44.

Key words

peanut;Yeast two-hybrid;MYB;interact proteins

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LIU Yong-hui,SHEN Yi,SHEN Yue and CHEN Zhi-de. Yeast Two-Hybrid Screening of the Proteins Interacting withAhMYB44 from Peanut. Journal of Plant Genetic Resources. 2020, 21(1): 201-207 https://doi.org/10.13430/j.cnki.jpgr.20191029002

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Funding

Special Fund for Modern Agroindustry Technology Research System of China (CARS-13), Independent Innovation Fund of Agricultural Science and Technology of Jiangsu Province(cx(18)2015), Key Laboratory ofBiology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, P.R. China(KF2019011)
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