Genetic Diversity Analysis of Persimmon Germplasm Resources in Shaanxi Province

GUAN Chang-fei,HU Chao-qiong,WANG Meng-ke,RUAN Xiao-feng,WANG Ren-zi and YANG Yong

PDF(1387 KB)
PDF(1387 KB)
Journal of Plant Genetic Resources ›› 2019, Vol. 20 ›› Issue (5) : 1340-1348. DOI: 10.13430/j.cnki.jpgr.20181213001
Short Communications

Genetic Diversity Analysis of Persimmon Germplasm Resources in Shaanxi Province

  • GUAN Chang-fei, HU Chao-qiong, WANG Meng-ke, RUAN Xiao-feng, WANG Ren-zi, YANG Yong
Author information +
History +

Abstract

In order to decipher the genetic diversiy of persimmon germplasms, a total of 112 persimmon cultivars (Diospyros kaki) collected from Shaanxi province, 16 date plums (D. lotus) from Shaanxi and 11 persimmon cultivars from Henan province were investigated by using ten SCoT markers with high polymorphism and qualified reproducibility. The statistical assessments including polymorphisms, cluster analysis, principal coordinate analysis, population structure analysis and genetic diversity analysis was analyzed according to the geographic regions of genotypes. A total of 99 polymorphic marker loci were obtained from 10 pair SCoT primers. By cluster analysis, 16 date plums were assigned to an independent cluster, differing with the persimmon cultivars. Persimmon cultivars from Henan province and Shaanxi province are clustered together and have a close genetic distance. Although the genetic structure analysis implied an exchange on genetic components among 112 persimmon cultivars in various regions of Shaanxi Province, the genetic diversity remained limited. The cultivars from both Baoji and Xi'an cities represented the highest genetic diversity, while the lowest diversity was observed in these of Southern Shaanxi. Thus, the results could facilitate better understanding of genetic contributions in varieties, and provide a scientific theoretical basis for further investigating the persimmon resources in Shaanxi Province.

Key words

persimmon / germplasm resources / genetic diversity / SCoT / Shaanxi province

Cite this article

Download Citations
GUAN Chang-fei,HU Chao-qiong,WANG Meng-ke,RUAN Xiao-feng,WANG Ren-zi and YANG Yong. Genetic Diversity Analysis of Persimmon Germplasm Resources in Shaanxi Province. Journal of Plant Genetic Resources. 2019, 20(5): 1340-1348 https://doi.org/10.13430/j.cnki.jpgr.20181213001

References

[1] 杨勇, 阮小凤, 王仁梓, 李高潮. 柿种质资源及育种研究进展. 西北林学院学报, 2005, 20:133-137
[2] Wang R Z, Yang Y. Chinese persimmon germplasm resourse. Acta Horticulturae, 1997, 436:43-50
[3] Mahjbi A, Baraket G, Oueslati A, Salhi A. Start Codon Targeted (SCoT) markers provide new insights into the genetic diversity analysis and characterization of Tunisian Citrus, species. Biochemical Systematics & Ecology, 2015, 61:390-398
[4] Feng S G, He R F, Jiang M Y, Lu J J. Genetic diversity and relationships of medicinal Chrysanthemum morifolium revealed by start codon targeted (SCoT) markers. Scientia Horticulturae, 2016, 201:118-123
[5] Cong L, He X H, Hu C, Ou S J, Gao M P. Analysis of diversity and relationships among mango cultivars using Start Codon Targeted (SCoT) markers. Biochemical Systematics & Ecology, 2010, 38:1176-1184
[6] 袁王俊, 马寅峰, 董美芳, 韩远记, 张维瑞, 尚富德. 桂花SCoT标记体系的建立及其在遗传多样性分析中的应用. 园艺学报, 2015, 34:569-575
[7] 高岭, 冯尚国, 何仁锋, 赵妙玉, 王慧中. 兰属植物目标起始密码子(SCoT)遗传多样性分析. 园艺学报, 2013, 40:2026-2032
[8] 夏乐晗, 杨婷婷, 杨勇, 夏宏义, 张永芳, 王仁梓. 柿SCoT-PCR体系优化及品种遗传多样性分析. 西北植物学报, 2014, 34:473-480
[9] Yang Y, Yang T T, Jing Z B. Genetic diversity and taxonomic studies of date plum (Diospyros lotus L.) using morphological traits and SCoT markers. Biochemical Systematics & Ecology, 2015, 61: 253-259
[10] Deng L, Liang Q, He X, Luo C, Chen H, Qin Z. 2015. Investigation and analysis of genetic diversity of Diospyros germplasms using SCoT molecular markers in Guangxi. PLoS ONE, 10: 8
[11] 唐冬兰, 胡燕, 龚榜初, 张青林, 罗正荣. 基于SCoT与IRAP标记的金枣柿实生后代遗传变异分析及其生殖特性. 果树学报, 2016, 33:934-942
[12] Doyle J J, Doyle J L. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin. 1987, 19:11-15
[13] Rolf J F. NTSYS-PC, Numerical taxonomy and multivariate analysis system. Version 2.11T Exeter Software. Setauket, NY, USA, 2000
[14] Pritchard J K, Stephens M, Donnelly P. Inference of population structure using multilocus genotype data. Genetics, 2000, 155:945-959
[15] Yeh F, Boyle T. Population genetic analysis of co-dominant and dominant markers and quantitative traits. Belgian Journal of Botany, 1997, 129:157
[16] 杨本芸. 桃、李、杏、核桃不同品种的SSR指纹图谱构建. 硕士学位论文, 河北农业大学, 2006
[17] 王文江. 柿(Diospyros kaki Thunb.)优良品种AFLP指纹图谱构建及遗传多样性研究. 博士学位论文, 河北农业大学, 2004

Funding

China Postdoctoral Science Foundation,The National Natural Science Foundation of China (General Program, Key Program, Major Research Plan)
Share on Mendeley
PDF(1387 KB)

36

Accesses

0

Citation

Detail

Sections
Recommended

/