大豆ERF耐盐基因的鉴定和驯化分析

高超升,袁嘉志,植健怡,程玉汉,董利东,程群

植物遗传资源学报. 2024, 25(1): 30-38

PDF(1882 KB)
PDF(1882 KB)
植物遗传资源学报 ›› 2024, Vol. 25 ›› Issue (1) : 30-38. DOI: 10.13430/j.cnki.jpgr.20230510002
论文

大豆ERF耐盐基因的鉴定和驯化分析

  • 高超升1,袁嘉志1,植健怡1,程玉汉2,董利东1,程群1
作者信息 +

Identification and Domestication Analysis of ERF Salt Tolerance Genes in Soybean

  • GAO Chaosheng1, YUAN Jiazhi1, ZHI Jianyi1, CHENG Yuhan2, DONG Lidong1, CHENG Qun1
Author information +
History +

摘要

土地盐渍化对大豆的产量和品质产生了极大的负面影响,培育耐盐大豆品种是改善和提高盐胁迫下大豆产量和品质的有效途径之一。ERF转录因子对植物响应逆境胁迫十分重要,但在大豆中相关研究报道较少。基于已报道的盐胁迫处理下的RNA-seq数据、549份大豆重测序数据及耐盐指数数据,以及Soybean Expression Atlas数据库中大豆组织表达数据,在大豆中鉴定能够响应盐胁迫的ERF基因。同时,在549份大豆重测序数据中鉴定响应盐胁迫ERF基因的优异等位变异,并分析其驯化与人工选择规律。其中,鉴定出ERF158H1ERF166H2ERF170H1单倍型是优异的自然等位变异,能够显著促进大豆对盐的耐性。对优异等位变异的核苷酸多态性分析表明,ERF170H1在大豆驯化的过程中受到了微弱的人工选择,而ERF158H1ERF166H2在驯化的过程中发生了逐渐减少或丢失的现象。因此,本研究鉴定了大豆中响应盐胁迫的ERF基因及其优异等位变异,对丰富和完善大豆响应盐胁迫的分子机制具有重要意义,为培育耐盐大豆品种提供了重要的基因资源和育种方案。

Abstract

Soil salinization has a significant negative impact on the yield and quality of soybean. It is one of the effective ways to improve the yield and quality of soybeans under salt stress by breeding salt-tolerant soybean varieties. Plant ERF transcription factors play important roles in responses to biotic and abiotic stresses, whereas there are few relevant studies in soybean. Based on RNA-Seq datasets under salt stress treatment, 549 soybean resequencing data and salt tolerance index data, as well as the soybean tissue expression data in the Soybean Expression Atlas database, the soybean ERF genes responding to salt stress and their elite alleles were identified. Three ERF genes were identified with natural variations, among which ERF158H1ERF166H2 and ERF170H1 haplotypes were elite alleles showing significantly higher salt tolerance. Nucleotide polymorphism analysis of the elite alleles showed that ERF170H1 was detected with weakly artificially selection signals during soybean domestication, while ERF158H1 and ERF166H2 were gradually reduced or lost during domestication process. Therefore, eight ERF genes were identified likely being involved in the response of soybean to salt stress, and three of them were found to have elite alleles that can significantly promote soybean salt tolerance. Our findings provide a great significance to enrich and improve the molecular mechanism of soybean response to salt stress and breeding programs for salt tolerant soybean varieties.

关键词

大豆 / 乙烯响应因子 / 耐盐 / 生物信息学 / 人工选择

Key words

soybean / ERF / salt tolerance / bioinformatics / artificial selection

引用本文

导出引用
高超升,袁嘉志,植健怡,程玉汉,董利东,程群. 大豆ERF耐盐基因的鉴定和驯化分析. 植物遗传资源学报. 2024, 25(1): 30-38 https://doi.org/10.13430/j.cnki.jpgr.20230510002
GAO Chaosheng,YUAN Jiazhi,ZHI Jianyi,CHENG Yuhan,DONG Lidong,CHENG Qun. Identification and Domestication Analysis of ERF Salt Tolerance Genes in Soybean. Journal of Plant Genetic Resources. 2024, 25(1): 30-38 https://doi.org/10.13430/j.cnki.jpgr.20230510002

参考文献

[1]范王涛. 土壤盐碱化危害及改良方法研究. 农业与技术, 2020, 40(23): 114-116Fan W T. Research on the harm of soil salinization and its improvement methods. Agriculture and Technology, 2020, 40(23): 114-116
[2] Dong L D, Cheng Q, Fang C, Kong L P, Yang H, Hou Z H, Li Y L, Nan H Y, Zhang Y H, Chen Q S, Zhang C B, Kou K, Su T, Wang L S, Li S C, Li H Y, Lin X Y, Tang Y, Zhao X H, Lu S J, Liu B H, Kong F J. Parallel selection of distinct Tof5 alleles drove the adaptation of cultivated and wild soybean to high latitude. Molecular Plant, 2021, 15(2): 308-321
[3] Cheng Q, Gan Z R, Wang Y P, Lu S J, Hou Z H, Li H Y, Xiang H T, Liu B H, Kong F J, Dong L D. The soybean gene J contributes to salt stress tolerance by up-regulating salt-responsive genes. Frontiers in Plant Science, 2020, 11: 272
[4] Bahmani K, Noori S A S, Darband A I, Akbari A. Molecular mechanisms of plant salinity tolerance: A review. Australian Journal of Crop Science, 2015, 9(4): 321-336
[5] Parker M B, Gascho G J, Gaines T P. Chloride toxicity of soybeans grown on Atlantic Coast Flatwoods Soils 1. Agronomy Journal, 1983, 75(3): 439-443
[6] Wang F, Chen H W, Li Q T, Wei W, Li W, Zhang W K, Ma B, Bi Y D, Lai Y C, Liu X L, Man W Q, Zhang J S, Chen S Y. GmWRKY27 interacts with GmMYB174 to reduce expression of GmNAC29 for stress tolerance in soybean plants. The Plant Journal, 2015, 83(2): 224-236
[7] Masaru O T, Hideaki S. Ethylene-inducible DNA binding proteins that interact with an ethylene-responsive element. The Plant Cell, 1995, 7(2): 173-182
[8] Pan Y, Seymour G B, Lu C, Hu Z L, Chen X Q, Chen G P. An ethylene response factor (ERF5) promoting adaptation to drought and salt tolerance in tomato. Plant Cell Reports, 2012, 31(2): 349-360
[9] Zhai Y, Wang Y, Li Y J, Lei T T, Yan F, Su L T, Li X W, Zhao Y, Sun X, Li J W, Wang Q Y. Isolation and molecular characterization of GmERF7, a soybean ethylene-response factor that increases salt stress tolerance in tobacco. Gene, 2013, 513(1): 174-183
[10] Wang H T, Ni D Q, Shen J C, Deng S S, Xuan H D, Wang C C, Xu J Y, Zhou L, Guo N, Zhao J M, Xing H. Genome-wide identification of the AP2/ERF gene family and functional analysis of GmAP2/ERF144 for drought tolerance in soybean. Frontiers in Plant Science, 2022, 13: 848766
[11] Dong L D, Hou Z H, Li H Y, Li Z B, Fang C, Kong L P, Li Y L, Du H, Li T, Wang L S, He M L, Zhao X H, Cheng Q, Kong F J, Liu B H. Agronomical selection on loss-of-function of GIGANTEA simultaneously facilitates soybean salt tolerance and early maturity. Journal of Integrative Plant Biology, 2022, 64(10): 1866-1882
[12] Mccormack M L, Dickie I A, Eissenstat D M, Fahey T J, Fernandez C W, Guo D L, Helmisaari H S, Hobbie E A, Iversen C M, Jackson R B, Jaana L K, Norby R J, Phillips R P, Pregitzer K S, Pritchard S G, Rewald B, Zadworny M. Redefining fine roots improves understanding of below-ground contributions to terrestrial biosphere processes. The New Phytologist, 2015, 207(3): 505-518
[13] Wu Y, Li X, Zhang J N, Zhao H Q, Tan S L, Xu W H, Pan J Q, Yang F, Pi E X. ERF subfamily transcription factors and their function in plant responses to abiotic stresses. Frontiers in Plant Science, 2022, 13: 1042084
[14] Shen L, Zhao E P, Liu R E, Yang X. Transcriptome analysis of eggplant under salt stress: AP2/ERF transcription factor SmERF1 acts as a positive regulator of salt stress. Plants, 2022, 11(17): 2205
[15] John F D, Brandon S G, Bruce D S. The molecular genetics of crop domestication. Cell, 2006, 127(7): 1309-1321
[16] Wang M, Li W Z, Fang C, Xu F, Liu Y C, Wang Z, Yang R, Zhang M, Liu S L, Lu S J, Lin T, Tang J Y, Wang Y Q, Wang H R, Lin H, Zhu B G, Chen M S, Kong F J, Liu B H, Zeng D L, Jackson S A, Chu C C, Tian Z X. Parallel selection on a dormancy gene during domestication of crops from multiple families. Nature Genetics, 2018, 50(10): 1435-1441
[17] Tian J G, Wang C L, Xia J L, Wu L S, Xu G H, Wu W H, Li D, Qin W C, Han X, Chen Q Y, Jin W W, Tian F. Teosinte ligule allele narrows plant architecture and enhances high-density maize yields. Science, 2019, 365(6454): 658-664
[18]李保国.新时代下盐碱地改良与利用的科学之路.中国农业综合开发, 2022(1): 8-9Li B G. The scientific road to the improvement and utilization of saline alkali land in the new era. Agricultural Comprehensive Development in China, 2022(1): 8-9
[19]高继平,林鸿宣.水稻耐盐机理研究的重要进展——耐盐数量性状基因SKC1的研究.生命科学, 2005(6): 563-565Gao J P, Lin H X. Important progress in the study of salt tolerance mechanism in rice: Study on the SKC1 gene for quantitative salt tolerance traits. Chinese Bulletin of Life Sciences, 2005(6): 563-565
[20] Liaqat A, Alfatih A, Jan S U, Sun L Q, Zhao P X, Xiang C B. Transcription elongation factor AtSPT4-2 positively modulates salt tolerance in Arabidopsis thaliana. BMC Plant Biology, 2023, 23(1): 49
[21]陈筱冉,张铭笑,严建萍,刘燕蓉,张万军.紫花苜蓿MsCCD4基因克隆及耐盐功能鉴定.草地学报, 2022, 30(10): 2572-2580Chen X R, Zhang M X, Yan J P, Liu Y R, Zhang W J. Cloning and identification of salt tolerance function of Alfalfa MsCCD4. Acta Agrestia Sinica, 2022, 30(10): 2572-2580
[22] Hou Z H, Li Y L, Cheng Y H, Li W W, Li T, Du H, Kong F J, Dong L D, Zheng D F, Feng N J, Liu B H, Cheng Q. Genome-wide analysis of DREB genes identifies a novel salt tolerance gene in wild soybean (Glycine soja). Frontiers in Plant Science, 2022, 13: 821647
[23] Guan R X, Yu L L, Li X X, Chang R Z, Gilliham M, Qiu L J. Selection of the salt tolerance gene GmSALT3 during six decades of soybean breeding in China. Frontiers in Plant Science, 2021, 12: 794241
PDF(1882 KB)

文章所在专题

玉米

Accesses

Citation

Detail

段落导航
相关文章

/