广东湛江蔗区甘蔗白条病的分子检测与鉴定

田夏红,赵建英,傅华英,施扬,苏俊波,刘建荣

植物遗传资源学报. 2022, 23(3): 731-737

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植物遗传资源学报 ›› 2022, Vol. 23 ›› Issue (3) : 731-737. DOI: 10.13430/j.cnki.jpgr.20211112001
论文

广东湛江蔗区甘蔗白条病的分子检测与鉴定

  • 田夏红1, 赵建英2, 傅华英2, 施扬2, 苏俊波3, 刘建荣1
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Molecular Detection and Identification of Xanthomonas albilineansCausing Leaf Scald of Sugarcane in Zhanjiang， Guangdong Province of China

  • TIAN Xia-hong1, ZHAO Jian-ying2, FU Hua-ying2, SHI Yang2, SU Jun-bo3, LIU Jian-rong1
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摘要

为明确粤西蔗区甘蔗白条病的病原类型，对 27 份新引进的甘蔗品种进行田间病害调查，并对 15 份疑似发病植株 的蔗茎样品进行病菌分离和 PCR 检测鉴定。2019 年和 2020 年田间调查结果显示，种植的 11 个甘蔗品种出现典型的白条病 症状，其中桂糖 08-1589、德蔗 12-88、福农 11-2907 和福农 09-7111 在新植蔗和宿根蔗上均有发生，自然发病率为 0.2%~9.4%。 利用白条黄单胞菌 Xanthomonas albilineans 特异性引物 XAF1/XAR1，对 15 份病样分离出来的病原细菌进行 PCR 检测，均可 获得 608 bp 的特异性扩增片段，所得序列完全一致，与来自中国福建、美国佛罗里达州和法国瓜德罗普岛的白条黄单胞菌一 致性为 100%，在系统进化树中处于同一分支，属于 PFGE-B 型基因型。本研究证实湛江地区 11 个甘蔗品种发生的白条病是 由白条黄单胞菌引起。

Abstract

To clarify the bacterial pathogen causing sugarcane leaf scald in west of Guangdong province， we conducted field surveys of leaf scald regarding 27 newly-introduced sugarcane varieties in two calendar years （2019 and 2020）. For 15 sugarcane stalk samples of suspected diseased plants，isolating the pathogen（s）and molecular identification by PCR were further performed. Based on the results of field trials，eleven sugarcane varieties were found with the typical symptom of leaf scald. Among them，the diseased plants from four varieties GT08-1589，DZ12-88，FN11-2907，and FN09-7111 were found in both the plant cane and ratoon cane，with incidence rates from 0.2% to 9.4%. The bacteria isolated from 15 diseased stalks were detected by PCR with the specific primers XAF1/XAR1 of Xanthomonas albilineans. These fragments are 608 bp in length and share 100% sequence identity to the published nucleotide sequences of X. albilineans isolates in China，USA，and France. These isolates were clustered in the same clade（PFGE-B）. Altogether，our results confirmed that leaf scald occurred in 11 cultivars from Zhanjiang sugarcane-planting region was caused by X. albilineans.

关键词

甘蔗；白条病；田间症状；分子检测；序列分析

Key words

sugarcane；leaf scald；field symptom；molecular detection；sequence analysis

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田夏红,赵建英,傅华英,施扬,苏俊波,刘建荣. 广东湛江蔗区甘蔗白条病的分子检测与鉴定. 植物遗传资源学报. 2022, 23(3): 731-737 https://doi.org/10.13430/j.cnki.jpgr.20211112001
TIAN Xia-hong,ZHAO Jian-ying,FU Hua-ying,SHI Yang,SU Jun-bo and LIU Jian-rong. Molecular Detection and Identification of Xanthomonas albilineansCausing Leaf Scald of Sugarcane in Zhanjiang， Guangdong Province of China. Journal of Plant Genetic Resources. 2022, 23(3): 731-737 https://doi.org/10.13430/j.cnki.jpgr.20211112001

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