水旱条件下小麦叶面积指数和叶绿素含量QTL定位

高雪,贾中立,林凯丽,侯学通,郑福兴,耿洪伟

植物遗传资源学报. 2021, 22(4): 1109-1119

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植物遗传资源学报 ›› 2021, Vol. 22 ›› Issue (4) : 1109-1119. DOI: 10.13430/j.cnki.jpgr.20210129002
论文

水旱条件下小麦叶面积指数和叶绿素含量QTL定位

  • 高雪1, 贾中立2, 林凯丽1, 侯学通1, 郑福兴1, 耿洪伟1
作者信息 +

QTL Mapping of Leaf Area Index and Chlorophyll Content in Wheat with Normal Irrigation and under Drought Stress

  • GAO Xue1, JIA Zhong-li2, LIN Kai-li1, HOU Xue-tong1, ZHENG Fu-xing1, GENG Hong-wei1
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摘要

利用春小麦 ‘Worrakatta’ × ‘Berkut’ 重组自交系（recombinant inbred line，RIL）的309份家系为材料，在正常灌溉和干旱胁迫两处理下，分别对小麦开花期、灌浆期和成熟期的叶面积指数（leaf area index，LAI），抽穗期、开花期和灌浆期的旗叶叶绿素含量（chlorophyll content，CC）进行了表型鉴定，并结合小麦50K SNP芯片进行QTL（quantitative trait locus）分析。结果表明：与正常灌溉相比，在干旱胁迫下LAI与CC均呈极显著降低趋势。两处理下各生育时期双亲及RIL群体的LAI、CC均呈现较大差异，存在明显的超亲分离现象。QTL定位结果显示，正常灌溉下在开花期和成熟期分别检测到2个和1个LAI相关QTL，分布于5BS、2BL和1BL染色体上，QLAI.xjau-5BS、QLAI.xjau-2BL.1和QLAI.xjau-1BL可解释表型变异的6.8%~8.2%；在抽穗期和开花期同时检测到与CC相关QTL位点QCC.xjau-1DS，该位点分布于1DS染色体，可解释表型变异的5.3%~5.8%。干旱胁迫下在成熟期共检测到1个LAI相关QTL位点QLAI.xjau-2BL.2 LAI，位于2BL染色体，可解释表型变异的13.8%。对上述所发现QTL位点进行基因挖掘，共筛选到7个与LAI和CC相关的候选基因，其中包括2个F-box家族蛋白和MYB相关基因、GATA相关基因、编码脱落酸受体基因、BTB/POZ相关基因及WUS相关基因各1个。这些基因参与调控作物的生长发育以及信号转导，还参与响应干旱等胁迫反应。本研究为小麦叶面积指数和叶绿素含量的基因发掘及分子育种提供了参考信息。

Abstract

Using a set of 309 recombinant inbred lines of the spring wheat ‘Worrakatta’ × ‘Berkut’ grown with normal irrigation and under drought stress, respectively, as materials, the phenotypes of leaf area index (LAI) at flowering, filling and ripening stages, and the flag leaf chlorophyll content (CC) at heading, flowering and filling stages were identified. QTL mapping was carried out with wheat 50K SNP chip. The results showed that LAI and CC decreased significantly under drought stress compared with normal irrigation treatment. It was found that LAI and CC of the parents and RIL lines were significantly different with an obvious phenomenon of transgressive segregation at different growth stages with the two treatments. Two and one LAI-related QTLs were detected at flowering stage and ripening stage, respectively, which were located on chromosomes 5BS, 2BL and 1BL with normal irrigation. QLAI.xjau-5BS, QLAI.xjau-2BL.1 and QLAI.xjau-1BL explained 6.8%-8.2% of the phenotypic variation. CC-related QTL QCC.xjau-1DS was detected at both heading and flowering stages, which was located on 1DS chromosome, explaining 5.3%-5.8% of the phenotypic variation. One LAI-related QTL, QLAI.xjau-2BL.2, located on 2BL chromosome, was detected at ripening stage under drought stress, explaining 13.8% of the phenotypic variation. Seven candidate genes related to LAI and CC were screened from the QTLs found in the study, which included two genes related to F-box family proteins and one gene each related to MYB, GATA, abscisic acid receptor, BTB/POZ and WUS. These genes are involved in regulating crop growth and development and signal transduction, as well as in response to drought and other stresses. This study provides reference information for gene discovery and molecular breeding of leaf area index and chlorophyll content in wheat.

关键词

小麦；叶面积指数；叶绿素含量；QTL；候选基因

Key words

wheat / leaf area index / chlorophyll content / QTL / candidate genes

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高雪,贾中立,林凯丽,侯学通,郑福兴,耿洪伟. 水旱条件下小麦叶面积指数和叶绿素含量QTL定位. 植物遗传资源学报. 2021, 22(4): 1109-1119 https://doi.org/10.13430/j.cnki.jpgr.20210129002
GAO Xue,JIA Zhong-li,LIN Kai-li,HOU Xue-tong,ZHENG Fu-xing and GENG Hong-wei. QTL Mapping of Leaf Area Index and Chlorophyll Content in Wheat with Normal Irrigation and under Drought Stress. Journal of Plant Genetic Resources. 2021, 22(4): 1109-1119 https://doi.org/10.13430/j.cnki.jpgr.20210129002

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