小麦耐热分子标记Xwmc44的鉴定及分析

刘子会,刘畅,彭义峰,李国良,张华宁,张红梅,段硕楠,郭秀林

植物遗传资源学报. 2021, 22(1): 95-101

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植物遗传资源学报 ›› 2021, Vol. 22 ›› Issue (1) : 95-101. DOI: 10.13430/j.cnki.jpgr.20200409002
论文

小麦耐热分子标记Xwmc44的鉴定及分析

  • 刘子会1, 刘畅2, 彭义峰3, 李国良1, 张华宁1, 张红梅1, 段硕楠1, 郭秀林1
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Identification and Analysis of Heat-tolerant Molecular Marker Xwmc44 in Wheat

  • LIU Zi-hui1, LIU Chang2, PENG Yi-feng3, LI Guo-liang1, ZHANG Hua-ning1, ZHANG Hong-mei1, DUAN Shuo-nan1, GUO Xiu-lin1
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摘要

耐热育种是我国小麦育种的一个重要目标。寻找与目标性状关联的分子标记，可克服常规育种的盲目性，提高分 子标记辅助选择育种的准确性。本研究对 169 份小麦品种（系）的千粒重、籽粒长度、籽粒宽度、籽粒表面积和籽粒周长 5 个 籽粒相关性状的耐热指数进行鉴定，通过简单关联分析获得耐热相关分子标记 Xwmc44。聚丙烯酰胺凝胶电泳分析表明： Xwmc44 具有 I、 II、 III 3 个等位变异类型。 I 型等位变异与千粒重、籽粒宽度、籽粒表面积和籽粒周长 4 个籽粒性状的热感指 数显著正相关（ P<0.05）， I 型等位变异小麦品种（系）的热感指数平均值大于 1； II 型等位变异与千粒重热感指数极显著负相 关（ P<0.01），与籽粒宽度和籽粒周长 2 个籽粒性状的热感指数显著负相关（ P<0.05）， II 型小麦品种（系）的耐热指数平均值 小于 1，为优良等位变异； III 型等位变异与小麦籽粒性状热感指数都不相关。研究结果表明： Xwmc44 与小麦耐热性密切相 关，其中 II 型等位变异是耐热优良等位变异。这为小麦耐热分子育种提供了参考信息。

Abstract

High temperature at reproductive stage destabilizes grain yield and quality especially in warm climate wheat production region. Breeding for heat-tolerant wheat varieties is an important target of wheat breeding in China， while identification of the molecular markers associated with the heat tolerant traits can accelerate this process. The heat-tolerant indexes of five kernel traits including thousand kernel weight， kernel length and kernel width， kernel surface area and kernel circumference were investigated by taking use of 169 wheat varieties（ lines）， and genotyping with SSR marker Xwmc44 identified three allelic variants I， II and III. A significant positive correlation between variant I and heat-tolerant indexes of four kernel traits（ thousand kernel weight， kernel width， kernel surface area and kernel circumference） was observed. The average values of heat-tolerant indexes of kernel traits in wheat varieties（ lines） with I allelic variation was greater than 1. The wheat varieties with variant II was negatively correlated with the heat tolerance index of thousand kernel weight （ P<0.01）， and negatively correlated with the heat-tolerance index of two kernel traits including kernel width and kernel circumference（ P<0.05） . The average value of heat-tolerance indexes of kernel traits in wheat varieties （ lines） with II allelic variation was less than 1. No detectable association was observed in variant III with the heat-tolerance indexes. These results showed that Xwmc44 was related to heat tolerance of wheat， and II allelic variation was a superior allele for heat tolerance of wheat. This will provide more information for heat-tolerant wheat breeding with molecular markers.

关键词

小麦；等位变异 / 分子标记 / 耐热性

Key words

wheat (Triticum aestivum L.) / allelic variation / molecular marker / heat tolerance

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刘子会,刘畅,彭义峰,李国良,张华宁,张红梅,段硕楠,郭秀林. 小麦耐热分子标记Xwmc44的鉴定及分析. 植物遗传资源学报. 2021, 22(1): 95-101 https://doi.org/10.13430/j.cnki.jpgr.20200409002
LIU Zi-hui,LIU Chang,PENG Yi-feng,LI Guo-liang,ZHANG Hua-ning,ZHANG Hong-mei,DUAN Shuo-nan and GUO Xiu-lin. Identification and Analysis of Heat-tolerant Molecular Marker Xwmc44 in Wheat. Journal of Plant Genetic Resources. 2021, 22(1): 95-101 https://doi.org/10.13430/j.cnki.jpgr.20200409002

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