大豆蛋白含量新位点qPRO-19-1 的定位

武阳春,郭兵福,谷勇哲,栾晓燕,邱红梅,刘鑫磊,李海燕,邱丽娟

植物遗传资源学报. 2021, 22(1): 139-148

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植物遗传资源学报 ›› 2021, Vol. 22 ›› Issue (1) : 139-148. DOI: 10.13430/j.cnki.jpgr.20200226001
论文

大豆蛋白含量新位点qPRO-19-1 的定位

  • 武阳春1,2, 郭兵福3, 谷勇哲 2,栾晓燕 4,邱红梅 5,刘鑫磊 4,李海燕 1,邱丽娟 1,2
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Mapping of a New Quantitative Locus qPRO-19-1 Associated with Seed Crude Protein Content in Soybean（ Glycine max L.）

  • WU Yang-chun1,2, GUO Bing-fu3, GU Yong-zhe2, LUAN Xiao-yan4, QIU Hong-mei5, LIU Xin-lei4, LI Hai-yan1, QIU Li-juan1,2
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摘要

大豆是重要的粮食作物和经济作物，其籽粒蛋白约为 40%，是植物蛋白的重要来源之一。国产大豆主要用于食用，提高 大豆蛋白含量是主要的育种目标。因此，发掘大豆蛋白含量相关基因，对开发分子标记并培育高蛋白食用大豆具有重要意义。本 研究以低蛋白大豆品种中黄 35 为母本，以源自日本的高蛋白大豆十胜长叶为父本，构建了重组自交系（ RIL， recombination inbred lines）群体。利用集群分离分析法（ BSA， bulked segregant analysis）在 3 条染色体筛选出 9 个与蛋白含量相关的 SSR 标记，其中位 于 19 号染色体的 QTL 尚未见报道。进一步利用完备区间作图法（ ICIM-ADD）分析 RIL 群体 F2： 15 和 F2： 16，在 19 号染色体重复定 位了 1 个蛋白质含量相关 QTL qPRO-19-1，位于分子标记 SSR_19_38 和 SSR_19_59 之间， LOD 值分别为 3.43 和 3.98，贡献率分别 为 7.81% 和 14.87%，高蛋白等位基因来自于高蛋白亲本十胜长叶。 qPRO-19-1 的定位区间长度为 385 kb，共有注释基因 36 个。本 研究定位了蛋白质含量相关的新位点 qPRO-19-1，为大豆高蛋白基因的图位克隆及分子标记育种奠定了基础。

Abstract

Soybean is an economically-important food crop that contains about 40% of grain protein. The soybeans harvested from China are mainly used for food， and breeding for higher protein content is one of the main targets in soybean. Exploration of the genes related to soybean protein content and development of molecular markers are thus of great significance. In this study， a recombinant inbred line population was used by crossing a low protein soybean variety‘ ZH35’ （ female parent） with the high protein accession‘ Tokachi nagaha’ （ male parent） that was introduced from Japan. With the Bulked Segregant Analysis（ BSA） analysis， nine SSR markers that associated to protein content were identified on three chromosomes， including chromosome 19 where no QTL was previously reported. This QTL qPRO-19-1 was allocated using an inclusive complete interval mapping method（ ICIM-ADD） to the genetic interval of the molecular markers SSR_19_38 and SSR_19_59. This elite allele， which was derived from‘ Tokachi Nagaha’ ， could explain 7.81% and 14.87% of phenotypic variations in F2： 15and F2： 16 with the LOD values of 3.43 and 3.98， respectively. The physical interval of qPRO-19-1 expanded 385 kb that harbored 36 annotated genes. Collectively， the genetic mapping of the new QTL qPRO-19-1 laid the foundation for map-based cloning gene and molecular marker assisted breeding for high protein soybean varieties.

关键词

大豆 / 重组自交系 / 蛋白质含量 / QTL

Key words

soybean； RIL； protein content； QTL

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武阳春,郭兵福,谷勇哲,栾晓燕,邱红梅,刘鑫磊,李海燕,邱丽娟. 大豆蛋白含量新位点qPRO-19-1 的定位. 植物遗传资源学报. 2021, 22(1): 139-148 https://doi.org/10.13430/j.cnki.jpgr.20200226001
WU Yang-chun,GUO Bing-fu,GU Yong-zhe,LUAN Xiao-yan,QIU Hong-mei,LIU Xin-lei,LI Hai-yan and QIU Li-juan. Mapping of a New Quantitative Locus qPRO-19-1 Associated with Seed Crude Protein Content in Soybean（ Glycine max L.）. Journal of Plant Genetic Resources. 2021, 22(1): 139-148 https://doi.org/10.13430/j.cnki.jpgr.20200226001

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