重庆地区新收集烟草种质资源的鉴定评价与整理编目

李媛,吕洪坤,张兴伟,杨爱国,冯全福,戴培刚,刘国祥,佟英

植物遗传资源学报. 2020, 21(4): 896-907

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植物遗传资源学报 ›› 2020, Vol. 21 ›› Issue (4) : 896-907. DOI: 10.13430/j.cnki.jpgr.20191019001
论文

重庆地区新收集烟草种质资源的鉴定评价与整理编目

  • 李媛1, 吕洪坤2, 张兴伟1, 杨爱国1, 冯全福1, 戴培刚1, 刘国祥1, 佟英1
作者信息 +

Evaluation and Cataloguing of Newly Collected Tobacco Germplasm Resources in Chongqing, China

  • LI Yuan1, LV Hong-kun2, ZHANG Xing-wei1, YANG Ai-guo1, FENG Quan-fu1, DAI Pei-gang1, LIU Guo-xiang1, TONG Ying1
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摘要

为将“第三次全国农作物种质资源普查与收集行动”重庆项目组收集到的 63 份烟草种质资源入国家种质资源库进 行编目和保存，对其进行田间鉴定和拍照记录。形态特征和主要农艺性状的遗传多样性分析表明，供试种质的数量性状和质 量性状遗传多样性丰富，质量性状以叶形的多样性指数最高，数量性状中叶长、叶宽和茎围的多样性指数较高。聚类分析将 新收集烟草种质资源分为 4 个类群，其中第Ⅰ类群以黄花烟为主，第Ⅲ类群的植株最高、叶片最大，可用于高产育种。主要 病毒病的抗性鉴定发现，有 11 份种质 TMV 和 CMV 的抗性均较强，其中 2 份种质对 TMV 免疫且高抗 CMV，可用于抗病育 种。另外，通过田间表型和 SSR 分子标记对拟编目资源进行鉴定整理，剔除了重复的种质资源。因此，烟草种质资源的普查 收集工作对于丰富国家烟草种质资源库和新品种选育具有重大意义。

Abstract

In order to catalog and preserve 63 tobacco germplasm accessions, which were collected by Chongqing Project Group in a frame of the Third National Survey and Collection Action on Crop Germplasm Resources, we conducted the field experiments for identifying the phenotypic and marker-assisted genetic diversity. Morphological characteristics and main agronomic traits were surveyed and analyzed. These tested germplasm accessions represented abundant genetic diversity at both quantitative traits and qualitative traits. Specially for qualitative traits, leaf shape had the highest diversity index, followed by Leaf length, leaf width and stem girth. Clustering analysis suggested four groups of these newly-collected tobacco germplasm accessions. The germplasms resided to the first group were mainly rustica, while the germplasms from the third group showed higher plant height and bigger leaves with a potential for high-yield breeding. Tests for disease resistance indicated that 11 accessions were resistant to TMV and CMV. Particularly, two of them were free of TMV infection and highly resistant to CMV, which could be used for disease resistance breeding. By taking use of field phenotypic datasets and genotyping results using SSR markers, the germplasm accessions showing identical were excluded for cataloglization and conservation into the National Bank of Tobacco Germplasm Resources. Therefore, survey and collection of tobacco germplasm resources is of great significance, in order to enrich the genetic diversity of Tobacco Germplasm Resources and benefit for tobacco breeding.

关键词

烟草 / 种质资源 / 遗传多样性 / 分子鉴定

Key words

tobacco / germplasm resources / genetic diversity / molecular identification

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李媛,吕洪坤,张兴伟,杨爱国,冯全福,戴培刚,刘国祥,佟英. 重庆地区新收集烟草种质资源的鉴定评价与整理编目. 植物遗传资源学报. 2020, 21(4): 896-907 https://doi.org/10.13430/j.cnki.jpgr.20191019001
LI Yuan,LV Hong-kun,ZHANG Xing-wei,YANG Ai-guo,FENG Quan-fu,DAI Pei-gang,LIU Guo-xiang and TONG Ying. Evaluation and Cataloguing of Newly Collected Tobacco Germplasm Resources in Chongqing, China. Journal of Plant Genetic Resources. 2020, 21(4): 896-907 https://doi.org/10.13430/j.cnki.jpgr.20191019001

参考文献

张兴伟,王志德,牟建民,刘艳华,任民,高加明.我国烟草种质资源现状与展望.中国烟草科学, 2009,30(6):78-83
Zhang X W, Wang Z D, Mu J M, Liu Y H, Ren M, Gao J M. Status and Prospects of Tobacco Germplasm in China. Chinese Tobacco Science, 2009, 30(6):78-83
任民,王志德,牟建民,刘艳华,张兴伟.我国烟草种质资源的种类与分布概况.中国烟草科学, 2009,30(z1):8-14
Ren M, Wang Z D, Mu J M, Liu Y H, Zhang X W. Overview of Species and Distribution of Tobacco Germplasm Resources in China. Chinese Tobacco Science, 2009, 30(z1):8-14
张兴伟,冯全福,杨爱国,任民,佟英,邢丽敏,王志德.中国烟草种质资源分发利用情况分析.植物遗传资源学报, 2016,17(3):507-516
Zhang X W, Feng Q F, Yang A G, Ren M, Tong Y, Xing L M, Wang Z D. Analysis of Tobacco(Nicotiana tabacum L.)Germplasm Distribution and Utilization in China. Journal of Plant Genetic Resources, 2016, 17(3):507-516
屈旭,焦禹顺,李毅君,王同华,刘少云,刘国祥,佟英,张兴伟.湖南地区新收集烟草种质资源的鉴定与遗传多样性分析.植物遗传资源学报, 2018,19(6):1117-1125
Qu X, Jiao Y S, Li Y J, Wang T H, Liu S Y, Liu G X, Tong Y, Zhang X W. Identification and Genetic Diversity Analysis of Newly Collected Tobacco Germplasm Resources in Hunan Province. Journal of Plant Genetic Resources, 2018, 19(6):1117-1125
刘国祥,邹昆晏,任民,佟英,冯全福,杨爱国,戴培刚,张兴伟.77份新收集烟草种质资源的鉴定评价与整理编目.植物遗传资源学报, 2018,19(2):212-224
Liu G X, Zou K Y, Ren M, Tong Y, Feng Q F, Yang A G, Dai P G, Zhang X W. Evaluation and Cataloguing of 77 Newly-collected of Tobacco Germplasm Resources. Journal of Plant Genetic Resources, 2018, 19(2):212-224
董昕,刘剑飞,杨华,张晓春,张丕辉,官玲,余雪源,杨明,张继君,张谊模,张云贵,范彦,李淑君.重庆地区玉米种质资源调查与收集.植物遗传资源学报, 2018,19(2):203-211
Dong X, Liu J F, Yang H, Zhang X C, Zhang P H, Guan L, Yu X Y, Yang M, Zhang J J, Zhang Y M, Zhang Y G, Fan Y, Li S J. Field Survey and Collection of Maize Germplasm Resources in Chongqing, China. Journal of Plant Genetic Resources, 2018, 19(2):203-211
高佳,黄娟,冉启凡,严娟,董昕,杨海健,张晓春,范彦,张谊模,张云贵,高爱农.重庆部分地区的种质资源调查荞麦篇.植物遗传资源学报, 2017,18(3):595-601
Gao J, Huang J, Ran Q F, Yan J, Dong X, Yang H J, Zhang X C, Fan Y, Zang Y M, Zhang Y G, Gao A N. Investigation of Crops Resources in Chongqing Municipality in the View of Buckwheat Species. Journal of Plant Genetic Resources, 2017, 18(3):595-601
Bindler G, Plieske J, Bakaher N, Gunduz I, Ivanov N, Van der Hoeven R, Ganal M, Donini P. A high density genetic map of tobacco (Nicotiana tabacum L.) obtained from large scale microsatellite marker development. Theoretical and Applied Genetics, 2011, 123(2):219-230
徐军,刘艳华,任民,牟建民,张兴伟,陈雅琼,王志德.部分普通烟草种质资源的SSR标记与指纹图谱分析.中国烟草科学, 2011,32(2):62-65
Xu J, Liu Y H, Ren M, Mu J M, Zhang X W, Chen Y Q, Wang Z D. SSR Fingerprint Map Analysis of Tobacco Germplasms. Chinese Tobacco Science, 2011, 32(2):62-65
陈芳,徐世晓,李晓辉,刘超,周建飞,王袁,田培,杨铁钊.基于SSR标记的80份烟草种质指纹图谱的构建及遗传多样性分析.作物杂志, 2019,(1):22-31
Chen F, Xu S X, Li X H, Liu C, Zhou J F, Wang Y, Tian P, Yang T Z. Construction of Molecular Fingerprinting and Analysis of Genetic Diversity for 80 Tobacco (Nicotiana tabacum) Germplasms Based on SSR Markers. Crops, 2019, (1):22-31
孙九喆,杨金初,苏东嬴,王二彬,王君婷,张顺峰,李萌,马林.基于SSR标记的初烤烟叶品种快速鉴别.烟草科技, 2019,52(3):26-32
Sun J Z, Yang J C, Su D Y, Wang E B, Wang J T, Zhang S F, Li M, Ma L. Rapid identification of cured tobacco leaf varieties based on SSR markers. Tobacco Science and Technology, 2019, 52(3):26-32
耿歆淇,杨惠娟,秦艳青,杨兴有,赵世民,史宏志.基于不同烟草类型基因组重测序的烟草SSR标记的开发和应用.作物杂志, 2019,(2):84-89
Geng X Q, Yang H J, Qin Y Q, Yang X Y, Zhao S M, Shi H Z. Development and Application of Tobacco SSR Markers Based on Genome Re-Sequencing of Different Tobacco Types. Crops, 2019,(2):84-89
姜洪甲,邢世东,马维广.抗TMV烤烟种质资源材料筛选与利用研究初报.中国烟草科学, 2009,30(z1):53-55
Jiang H J, Xing S D, Ma W G. Selection and Utilization of Germplasm Material for Anti-TMV Flue-cured Tobacco. Chinese Tobacco Science, 2009, 30(z1):53-55
王志德,王元英,牟建民.烟草种质资源描述规范和数据标准.北京:中国农业出版社, 2006.
Wang Z D, Wang Y Y, Mu J M. Descriptors and Data Standard for Tobacco (Nicotiana spp.). Beijing: China Agriculture Press, 2006.
张倩男,王晓敏,芮文婧,胡学义,胡新华,付金军,高艳明,李建设,张亚红.基于表型性状及抗病标记的番茄种质资源遗传多样性分析.西南农业学报, 2018(1):14-21.
Zhang Q N, Wang X M, Rui W J, Hu X Y, Fu J J, Gao Y M, Li J S, Zhang Y H. Genetic Diversity Analysis of Tomato Germplasm Resources Based on Phenotypic Traits and Disease Resistance Markers. Southwest China Journal of Agricultural Sciences, 2018(1):14-21.
郑殿升,杨庆文,刘旭.中国作物种质资源多样性.植物遗传资源学报, 2011,12(4):497-500,506
Zheng D S, Yang Q W, Liu X. Diversity of Crop Germplasm Resources in China. Journal of Plant Genetic Resources, 2011,12(4):497-500,506
Semagn K, Babu R, Hearne S and Olsen M. Single nucleotide polymorphism genotyping using Kompetitive Allele Specific PCR (KASP): overview of the technology and its application in crop improvement. Mol Breeding, 2014, 33:1-14
Hamblin M T, Warburton M L and Buckler E S. Empirical Comparison of Simple Sequence Repeats and Single Nucleotide Polymorphisms in Assessment of Maize Diversity and Relatedness. Plos One, 2017, 2(12):e1367
Van Inghelandt D, Melchinger A E, Lebreton C and Stich B. Population structure and genetic diversity in a commercial maize breeding program assessed with SSR and SNP markers. Theor Appl Genet, 2010, 120: 1289-1299
王雯玥,杨涛,谭光万,杨建仓.作物种质资源研究与利用.中国种业, 2018,(1):14-20
Wang W Y, Yang T, Tan G W, Yang J C.Research and Utilization of Crop Germplasm Resources.China Seed Industry, 2018, (1):14-20
张兴伟,王志德,张久权,任民,牟建民,刘艳华.中国烟草种质资源信息网的开发与应用.中国烟草科学, 2009,30(z1):32-36
Zhang X W, Wang Z D, Zhang J Q, Ren M, Mu J M, Liu Y H. Development and Application of Tobacco Germplasm Resource Information System in China. Chinese Tobacco Science, 2009, 30(z1):32-36
任民,张兴伟,王志德,刘艳华,牟建民.基于Google Maps API的烟草种质资源WebGIS开发研究.植物遗传资源学报, 2010,11(5):522-526
Ren M, Zhang X W, Wang Z D, Liu Y H, Mu J M. Web Geographic Information System of Chinese Tobacco Germplasm Resources Based on Google Maps API. Journal of Plant Genetic Resources, 2010, 11(5):522-526
任民,张兴伟,张久权,王志德,刘艳华,牟建民.烟草种质资源在线共享系统的开发应用.中国烟草科学, 2011,32(4):51-55
Ren M, Zhang X W, Zhang J Q, Wang Z D, Liu Y H, Mu J M. Development of Online Order System for Tobacco Germplasm Resource Sharing. Chinese Tobacco Science, 2011, 32(4):51-55
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