玉米种质资源的抗倒伏性评价及鉴定指标筛选

郑云霄,刘文斯,赵永锋,贾晓艳,祝丽英,黄亚群,陈景堂,郭晋杰

植物遗传资源学报. 2019, 20(6): 1588-1596

PDF(6700 KB)
PDF(6700 KB)
植物遗传资源学报 ›› 2019, Vol. 20 ›› Issue (6) : 1588-1596. DOI: 10.13430/j.cnki.jpgr.20190418001
论文

玉米种质资源的抗倒伏性评价及鉴定指标筛选

  • 郑云霄, 刘文斯, 赵永锋, 贾晓艳, 祝丽英, 黄亚群, 陈景堂, 郭晋杰
作者信息 +

Evaluation of Lodging Resistance and Selection of Identification Indexes of Maize Germplasm Resources

  • ZHENG Yun-xiao, LIU Wen-si, ZHAO Yong-feng, JIA Xiao-yan, ZHU Li-ying, HUANG Ya-qun, CHEN Jing-tang, GUO Jin-jie
Author information +
History +

摘要

倒伏是影响玉米高产稳产的主要问题之一。本研究以 181 份玉米自交系为试验材料,对 22 个与抗倒伏性紧密相关的性状进行测定,采用相关性分析、主成分分析、聚类分析、逐步判别分析和岭回归分析等方法,综合评价玉米自交系的抗倒伏性。相关性分析的结果表明,22 个性状间存在不同程度的相关性。主成分分析的结果表明,前 7 个主成分代表了 181 份玉米自交系的 22 个性状 74.460%的信息,其贡献率分别为 25.700%、12.369%、9.782%、8.159%、7.782%、5.490%和 5.177%。聚类分析的结果表明,当遗传距离为 3.5 时,181 份玉米自交系被聚为 4 大类,第Ⅰ类有 35 份自交系、第Ⅱ类有 47 份自交系、第Ⅲ类有 49 份自交系、第Ⅳ类有 50 份自交系。逐步判别分析的结果表明,175 份玉米自交系被正确判别,判对概率为96.69%;6 份玉米自交系被误判,误判率为3.31%,这说明聚类分析的结果是准确可靠的。进一步利用岭回归方法筛选出半纤维素含量、穿刺强度、第三节间长度、第三节间直径、穗位高和维管束总面积 6 个性状,建立了可靠的玉米自交系抗倒伏性评价回归模型。在 181 份玉米自交系中,以 AHU24、e220、7026B 等 35 份自交系的抗倒伏能力最强。本研究结果为玉米抗倒伏种质资源的遗传改良和组配抗倒伏杂交种提供借鉴。

Abstract

Lodging is one of the main problems affecting the maize production and extension. In this study, 181 maize inbred lines were used for determining 22 characters associating to lodging resistance. Statistical analysis using the correlation analysis, the principal component analysis, the cluster analysis, the stepwise discriminate analysis and the ridge regression analysis was deployed for data interpretation. The results of the correlation analysis showed that 22 characters existed in varying degrees of correlation. The first seven principal components explained 74.460% of the phenotypic variation with the contribution rates of 25.700%, 12.369%, 9.782%, 8.159%, 7.782%, 5.490% and 5.177%. Four categories were clustered in the 181 maize inbred lines at a genetic distance of 3.5. Among them, the first classⅠhas 35 inbred lines, the classⅡhas 47 inbred lines, the class Ⅲ has 49 inbred lines, the class Ⅳ has 50 inbred lines. The result of stepwise discriminant analysis showed that 175 maize inbred lines were correctly discriminated and the identification rate was 96.69%. Six maize inbred lines were incorrectly discriminated and the identification rate was 3.31%, which means that the result of cluster analysis is accurate and reliable. The hemicellulose content, rind penetrometer strength, third internode length, third internode diameter, ear height and area of total vascular bundles were selected and the regression model of lodging resistance of maize inbred line was established by using the ridge regression method. 35 inbred lines, such as AHU 24, e220 and 7026B, showed the highest lodging resistance. Thus, the results provided a reference of the maize lodging resistant germplasm resources, which become valuable in selection of elite germplasm resources in breeding for the lodging resistance hybrids.

关键词

玉米;抗倒伏性;主成分分析;聚类分析;岭回归;综合评价

Key words

maize / lodging resistance / principal component analysis / cluster analysis / ridge regression / comprehensive evaluation

引用本文

导出引用
郑云霄,刘文斯,赵永锋,贾晓艳,祝丽英,黄亚群,陈景堂,郭晋杰. 玉米种质资源的抗倒伏性评价及鉴定指标筛选. 植物遗传资源学报. 2019, 20(6): 1588-1596 https://doi.org/10.13430/j.cnki.jpgr.20190418001
ZHENG Yun-xiao,LIU Wen-si,ZHAO Yong-feng,JIA Xiao-yan,ZHU Li-ying,HUANG Ya-qun,CHEN Jing-tang and GUO Jin-jie. Evaluation of Lodging Resistance and Selection of Identification Indexes of Maize Germplasm Resources. Journal of Plant Genetic Resources. 2019, 20(6): 1588-1596 https://doi.org/10.13430/j.cnki.jpgr.20190418001

参考文献

[1]刘志娟, 杨晓光, 吕硕, 王静, LIN XiaoMao. 东北三省春玉米产量差时空分布特征. 中国农业科学, 2017, 50(9): 1606-1616.LIU Z J, YANG X G, Lü S, WANG J, LIN X M.Spatial-temporal variations of yield gaps of spring maize in northeast China. Scientia Agricultura Sinica, 2017, 50(9): 1606-1616.
[2]程富丽, 杜雄, 刘梦星, 靳小利, 崔彦宏. 玉米倒伏及其对产量的影响. 玉米科学, 2011, 19(1): 105–108.CHENG F L, DU X, LIU M X, JIN X L, CUI Y H. Lodging of summer maize and the effects on grain yield. Maize Sciences, 2011, 19(1): 105–108.
[3]薛军, 王群, 李璐璐, 张万旭, 谢瑞芝, 王克如, 明博, 侯鹏, 李少昆. 玉米生理成熟后倒伏变化及其影响因素. 作物学报, 2018, 44(12): 1782-1792.XUE J, WANG Q, LI L L, ZHANG W X, XIE R Z, WANG K R, MING B, HOU P, LI S K. Changes of maize lodging after physiological maturity and its influencing factors. Acta Agronomica Sinica, 2018, 44(12): 1782-1792.
[4]Norboerg O S, Mason S C, Lowry S R. Ethephon influence on harvestable yield, grain quality, and lodging of corn. Agron J, 1988, 80: 768–772.
[5]Xu C L, Gao Y B, Tian B J, Ren J H, Meng Q F, Wang P. Effects of EDAH, a novel plant growth regulator, on mechanical strength, stalkvascular bundles and grain yield of summer maize at high densities. Field Crops Res, 2017, 200: 71–79.
[6]王元东, 段民孝, 邢锦丰, 王继东, 张春原, 张雪原, 赵久然. 玉米理想株型育种的研究进展与展望. 玉米科学, 2008, 03: 47—50.WANG Y D, DUAN M X, XING J F, WANG J D, ZHANG C Y, ZHANG X Y, ZHAO J R. Progress and prospect in ideal plant type breeding in maize. Maize Sciences, 2008, 03: 47—50.
[7]薛金涛,张保明,董志强,赵明.化学调控玉米抗倒及产量性状的效应研究.作物杂志, 2008, 4:72-76.XUE J T, ZHANG B M, DONG Z Q, ZHAO M. Effect of chemical regulation on lodging and yield of Maize. Crops, 2008, 4: 72-76.
[8]Fu X Q, Feng J, Yu B, Gao Y J, Zheng Y L, Yue B. Morphological, biochemical and genetic analysis of a brittle stalk mutant of maize inserted by mutator. Journal of Integrative Agriculture, 2013, 12(1):12-18.
[9]穆春华, 张发军, 李文才, 孙琦, 孟昭东. 玉米自交系茎秆显微结构及其与茎节抗折强度的相关与通径分析. 玉米科学, 2012, 20(5): 71-75.MU C H, ZHANG F J, LI W C, SUN Q, MENG Z D. Correlation and path analysis between node snap strength (NSS) and stalk microstructure of maize inbred line. Maize Sciences. 2012, 20(5): 71-75.
[10]任安然, 武丽芬, 关红辉, 杨莎, 监立强, 刘元峰, 杜宇茜,王姣, 郭晋杰, 陈景堂. 不同种植密度下玉米茎秆纤维性状和抗倒性相关分析. 植物遗传资源学报, 2017, 18(04): 653-664.REN A R, WU L F, GUAN H H, YANG S, JIAN L Q, LIU Y F, DU Y Q, WANG J, GUO J J, CHEN J T. Stalk Fiber Related Traits and Lodging Resistance Correlation Analysis under Different Planting Density in Maize. Journal of Plant Genetic Resources, 2017, 18(04): 653-664.
[11]武丽芬. 玉米茎秆抗倒相关性状的遗传效应分析. 保定: 河北农业大学, 2017.WU L F. Genetic effects analysis of stalk lodging resistance traits in maize. Baoding: Hebei Agricultural University, 2017.
[12]Huang C, Chen Q Y, Xu G H, Xu D Y, Tian J E, Tian F. Identification and fine mapping of quantitative trait loci for the number of vascular bundle in maize stem: QTL mapping for vascular bundle number. Journal of Integrative Plant Biology, 2015, 58(1).
[13]杨莎. 玉米自交系抗倒性鉴定及茎秆维管束性状QTL定位. 保定: 河北农业大学, 2016.YANG S. Identification of lodging-resistance and mapping of quantitative trait loci(QTL)for stalk vascular bundle in maize. Baoding: Hebei Agricultural University, 2016.
[14]王 军, 周美学, 许如根, 吕超, 黄祖六. 大麦耐湿性鉴定指标和评价方法研究. 中国农业科学, 2007, 40(10): 2145-2152.WANG J, ZHOU M X, XU R G, Lü C, HUANG Z L. Studies on selecting indices and evaluation methods for barley’s(Hordeum vulgare L.)waterlogging tolerance. Scientia Agricultura Sinica, 2007, 40(10): 2145-2152.
[15]田治国. 万寿菊属植物耐热性与抗旱性的评价及生长生理特性的研究. 陕西: 西北农林科技大学, 2012.TIAN Z G. Evaluation on heat and drought tolerance of marigold(Tagetes) and study on it's growth and physiological characteristics. Shanxi: Northwest A F University, 2012.
[16]林兆里, 许莉萍, 高三基, 徐金汉. 基于因子分析和聚类分析的甘蔗品种抗螟性评价. 热带作物学报, 2015, 36(07): 1312-1318.LIN Z L,XU L P, GAO S J, XU J H. Evaluation of Sugarcane Varieties Resistance to Borers Based on Factor Analysis and Cluster Analysis. Chinese Journal of Tropical Crops, 2015, 36(07): 1312-1318.
[17]付凤玲, 李晚忱, 潘光堂. 模糊隶属法对玉米苗期耐旱性的拟合分析. 干旱地区农业研究, 2003, 21(1): 83-85, 125.FU F L, LI W C, PAN G T. A fitting analysis on drought tolerance of maize seedling with fuzzy subordination method. Agricultural Research in the Arid Areas, 2003, 21(1): 83-85, 125.
[18]刘翔宇, 赵龙, 巴哈尔古丽?先木西, 彭华, 阿不都热衣木?玉拉音. 新疆陆地棉种质资源的综合评价. 中国农业科学, 2017, 50(24): 4679-4691.LIU X Y, ZHAO L, BAHARGUL.Xamxi, PENG H, ABDUREYIM.Ibrayim. Comprehensive evaluation of germplasm resources of upland cotton in Xinjiang. Scientia Agricultura Sinica, 2017, 50(24): 4679-4691.
[19]钟冲, 郭强. 费歇尔判别法及其应用. 西南交通大学学报, 2008,43(1): 136-141.QIAN C, GUO Q. Fisher discrimination method and its application. Journal of Southwest Jiaotong University, 2008,43(1): 136-141.
[20]丰光, 刘志芳, 李妍妍, 邢锦丰, 黄长玲. 玉米茎秆耐穿刺强度的倒伏遗传研究. 作物学报, 2009, 35(11): 2133-2138.FENG G, LIU Z F, LI Y Y, XING J F, HUANG C L. Genetics of lodging in tolerance to maize stem puncture. Acta Agronomica Sinica, 2009, 35(11): 2133-2138.
[21]冯海娟, 张善平, 马存金, 刘鹏, 董树亭, 赵斌, 张吉旺, 杨今胜. 种植密度对夏玉米茎秆维管束结构及茎流特性的影响. 作物学报, 2014, 40(8): 1435—1442.FENG H J, ZHANG S P, MA C J, LIU P, DONG S T, ZHAO B, ZHANG J W, YANG J S. Effect of plant density on microstructure of stalk vascular bundle of summer maize (Zea mays L.) and its characteristics of sap flow. Acta Agronomica Sinica, 2014, 40(8): 1435—1442.
[22]王永学, 张战辉, 刘宗华. 玉米抗倒伏性状的配合力效应及通径分析. 河南农业大学学报, 2011, 45(01): 1-6.WANG Y X, ZHANG Z H, LIU Z H. Combining ability and path analysis of lodging resistance traits in maize. Journal of Henan Agricultural University, 2011, 45(01): 1-6.
[23]Ching A, Dhugga K S, Appenzeller L, Meeley R, Bourett T M, Howard R J, Rafalski A. Brittle stalk 2 encodes a putative glycosylphosphatidylinositol-anchored protein that affects mechanical strength of maize tissues by altering the composition and structure of secondary cell walls. Planta, 2006, 224, 1174-1184.
[24]Appenzeller L, Doblin M, Barreiro R, Wang H Y, NIU X M, Kollipara K, Carrigan L, Tomes D, Chapman M, Dhugga K S. Cellulosesynthesis in maize: isolation and expression analysis of the cellulose synthase (Ces A) gene family. Cellulose, 2004, 11: 287-299.
[25]王枟刘, 王长进, 刘正, 李文阳. 72个玉米自交系抗倒性状的相关分析及评价. 安徽科技学院学报, 2016, 30(04): 26-30.WANG T L, WANG C J, LIU Z, LI W Y. Correlation analysis and evaluation in lodging resistance properties of 72 maize lines. Journal of Anhui Science and Technology University, 2016, 30(04): 26-30.
[26]何亮. 主成分分析在SPSS中的应用. 山西农业大学学报, 2007, 6(5): 20-22.HE L. Principal components analysis in SPSS. Journal of Shanxi Agricultural University(Social Science Edition, 2007, 6(5): 20-22.
[27]陈培峰, 王建平, 黄健, 朱勇良, 乔中英, 黄萌. 太湖地区香稻品种稻米品质性状相关和聚类分析. 江苏农业学报, 2013, 29(1): 1-7.CHEN P F, WANG J P, HUANG J, ZHU Y L, QIAO Z Y, HUANG M. Correlation and cluster analyses for grain quality traits in aromatic rice from Taihu Lake area. Jiangsu Journal of Agricultural Sciences, 2013, 29(1): 1-7.
[28]杨小翠, 詹秋文, 刘言龙, 李杰勤, 林平. 苏丹草与高梁的判别分析和聚类分析. 中国草地学报, 2016, 38(1): 41-46.YANG X C, ZHAN Q W, LIU Y L, LI J Q, LIN P. Discrimination analysis and cluster analysis of sorghum sudanense and S.bicolor. Chinese Journal of Grassland, 2016, 38(1): 41-46.
[29]陈森林, 黄曼艳, 林云, 陶红, 胡玉玲, 叶为民, 李攻科. 液相色谱结合多元统计分析多酚与烟叶香型的关系.分析化学: 1-13.CHEN S L, HUANG M Y, LIN Y, TAO H, HU Y L, YE W M, LI G K. Liquid chromatography coupled with multivariate statistics for investigation of relationship between polyphenols and aroma types of tobacco. Chinese Journal of Analytical Chemistry: 1-13.
[30]彭智, 李龙, 柳玉平, 刘惠民, 景蕊莲. 小麦芽期和苗期耐盐性综合评价. 植物遗传资源学报, 2017, 18(04): 638-645.PENG Z, LI L, LIU Y P, LIU H M, JING R L. Evaluation of salinity tolerance in wheat (Triticum aestium) genotypes at germination and seedling stages. Journal of Plant Genetic Resources, 2017, 18(04): 638-645.
[31]范娜, 白文斌, 彭之东, 赵建武. 粒用高粱耐盐种质资源鉴选与评价. 干旱地区农业研究, 2018, 36(03): 72-78.FAN N, BAI W B, PENG Z D, ZHAO J W. Identification and evaluation of salt-tolerant sorghum germplasm resources. Agricultural Research in the Arid Areas, 2018, 36(03): 72-78.
[32]郑崇兰, 王友富, 铁万祝, 晋一棠, 廖莉莉, 万幸, 祁正权. 四川攀西地区夏玉米主要农艺性状主成分与聚类分析. 贵州农业科学, 2018, 46(02): 9-13.ZHENG C L, WANG Y F, TIE W Z, JIN Y T, LIAO L L, WAN X, QI Z Q. Principal component and clustering analysis of main agronomic traits of different summer maize varieties in Panxi region, Sichuan. Guizhou Agricultural Sciences, 2018, 46(02): 9-13.
[33]Aktar S, Hossain N, Azam M G, Billah M, Biswas P L, Latif M A, Rohman M, Bagum S A, Uddin M S. Phenotyping of hybrid maize (Zea mays L.) at seedling stage under drought condition. American Journal of Plant Sciences, 2018(11): 2154-2169.
[34]刘洋. 玉米自交系抗倒伏性状的关联分析. 保定: 河北农业大学, 2014.LIU Y. Association analysis of lodging resistance traits of maize inbred lines. Baoding: Hebei Agricultural University, 2014.
[35]刘志斋, 吴迅, 刘海利, 李永祥, 李清超, 王凤格, 石云素, 宋燕春, 宋伟彬, 赵久然, 赖锦盛, 黎裕, 王天宇. 基于40个核心SSR标记揭示的820份中国玉米重要自交系的遗传多样性与群体结构. 中国农业科学, 2012, 45(11): 2107-2138.LIU Z Z, WU X, LIU H L, LI Y X, LI Q C, WANG F G, SHI Y S, SONG Y C, SONG W B, ZHAO J R, LAI J S, LI Y, WANG T Y. Genetic diversity and population structure of important Chinese maize inbred lines revealed by 40 core simple sequence repeats (SSRs). Scientia Agricultura Sinica, 2012, 45(11): 2107-2138.
PDF(6700 KB)

文章所在专题

玉米

资源与环境

41

Accesses

0

Citation

Detail

段落导航
相关文章

/