北方花生育成品种(系)分子标记鉴定及系谱分析

高斌,李洪珍,崔顺立,郭丽果,陈焕英,穆国俊,杨鑫雷,刘立峰

植物遗传资源学报. 2019, 20(6): 1472-1485

PDF(14587 KB)
PDF(14587 KB)
植物遗传资源学报 ›› 2019, Vol. 20 ›› Issue (6) : 1472-1485. DOI: 10.13430/j.cnki.jpgr.20181218003
论文

北方花生育成品种(系)分子标记鉴定及系谱分析

  • 高斌, 李洪珍, 崔顺立, 郭丽果, 陈焕英, 穆国俊, 杨鑫雷, 刘立峰
作者信息 +

Molecular Marker Identification and Pedigree Analysis for Peanut Cultivars (Lines) in Northern China

  • GAO Bin, LI Hong-zhen, CUI Shun-li, GUO Li-guo, CHEN Huan-ying, MU Guo-jun, YANG Xin-lei, LIU Li-feng
Author information +
History +

摘要

本研究利用从3964对引物中鉴定筛选到的57对SSR和6对AhTE转座元件引物对40份骨干亲本和113份北方花生育成品种（系）进行基因型扫描，共检测到267个等位位点，其中209个为多态性位点，多态性比率达78.28%。引物多态性信息含量（PIC）变幅为0.0754~0.9217，平均值为0.7134。利用基因型数据进行聚类分析，当遗传相似系数（GS）为0.62时，153份花生材料分为Ⅰ（2个亚类亚群，GS为0.67852）、Ⅱ（13个亚类亚群，GS为0.7746）两个类群；当GS为0.99时，63对核心引物可将153份种质资源完全分开，并构建了DNA指纹数据库。通过遗传贡献值分析出北方花生育成品种（系）的骨干亲本主要以伏花生、狮头企和沐阳大站秧为主，其中伏花生遗传贡献值最大（18.26），衍生品种最多（75份），而在河南、河北、山东等地的伏花生衍生品种比率分别为65.12%、70.83%、72.73%，且衍生品种比率与遗传贡献值呈正相关。本研究将为我国北方花生新品种鉴定提供更加科学的理论依据，为花生品种系谱修订奠定基础。

Abstract

In the present study, 113 peanut varieties in Northern China and 40 key breeding parental lines were subjected for molecular genotyping, by using 57 SSR markers and 6 AhTEtransposable element markers which come from 3964 candidate primers. Out of 267 detected alleles, 209 alleles showed polymorphisms with the polymorphic ratio of 78.28%. The PIC of all the primers ranged from 0.0754 to 0.9217 with an average of 0.7134. Subsequently, when GS (genetic similarity coefficient) = 0.62, the cluster analysis partitioned two groups including I (including 2 sub-groups, GS=0.6786) and II (including 13 sub-groups, GS=0.7674). When GS was 0.989, a total of 153 peanut cultivars were separated by 63 core primer pairs, and a DNA fingerprint database was established. By tracing the source of varieties, Fuhuasheng, Shitouqi, and Muyangdazhanyang as main backbone parents of peanut cultivars in Northern China, were dissected by calculating the genetic contribution value and the number of derived varieties of the backbone parents. Among them, the genetic contribution of Fuhuasheng was the largest (18.26% ), and the most derivative species (75 cultivars), while the ratio of derivatives in Henan, Hebei and Shandong provinces were 65.12 %, 70.83 %, and 72.73 %, respectively. The ratio of derivatives was positively correlated with the genetic contribution value. Thus, this study will provide a theoretical basis on the molecular identification of peanut cultivars (lines) in Northern China, which might be valuable on the guidance of classifying peanut pedigree.

关键词

花生 / 分子标记 / 指纹图谱 / 系谱分析

Key words

peanut / molecular markers / DNA fingerprint / pedigree analysis

引用本文

EndNote

Ris (Procite)

Bibtex

导出引用
高斌,李洪珍,崔顺立,郭丽果,陈焕英,穆国俊,杨鑫雷,刘立峰. 北方花生育成品种(系)分子标记鉴定及系谱分析. 植物遗传资源学报. 2019, 20(6): 1472-1485 https://doi.org/10.13430/j.cnki.jpgr.20181218003
GAO Bin,LI Hong-zhen,CUI Shun-li,GUO Li-guo,CHEN Huan-ying,MU Guo-jun,YANG Xin-lei and LIU Li-feng. Molecular Marker Identification and Pedigree Analysis for Peanut Cultivars (Lines) in Northern China. Journal of Plant Genetic Resources. 2019, 20(6): 1472-1485 https://doi.org/10.13430/j.cnki.jpgr.20181218003

参考文献

[1] 陈静, 胡晓辉, 苗华荣, 石运庆, 禹山林. SSR标记分析国家北方花生区试品种的遗传多样性[J]. 植物遗传资源学报, 2009, 10(3): 360-366Chen J, Hu X H, Miao H R, Shi Y Q, Yu S L. Analysis of genetic diversity of peanut (Arachis hypogaea L.) varieties under the region tests of north China based on SSR marker[J]. Journal of Plant Genetic Resources, 2009, 10(03): 360-366
[2] 殷冬梅, 王允, 尚明照, 崔党群. 花生优异种质的分子标记与遗传多样性分析[J]. 中国农业科学,2010, 43(11): 2220-2228Yin D M, Wang Y, Shang M Z, Cui D Q. Genetic diversity analysis of peanut genotypes based on molecular markers[J]. Scientia Agricultura Sinica, 2010, 43(11): 2220-2228
[3]周金超, 杨鑫雷, 崔顺立, 侯名语, 陈焕英, 穆国俊, 刘立峰. 花生SSR标记与农艺性状的相关性[J]. 作物学报, 2014, 40(7): 1197-1204Zhou J C, Yang X L, Cui S L, Hou M Y, Chen H Y, Mu G J, Liu L F. Correlation between SSR markers and agronomic traits in peanut (Arachis hypogaea L.) [J]. The Crop Journal. 2014. 40(7): 1197-1204
[4] Roder M, Wendehake K, Korzun V, Bredemeijer G, Laborie D, Bertrand L, Isaac P, Rendell S, Jackson J, Cooke R, Vosman B, Ganal M. Construction and analysis of a microsatellite-based database of European wheat varieties [J]. Theoretical Applied Genetics, 2002, 106(1): 67-73
[5] R?der M S, Korzun V, Wendehake K, Plaschke J, Tixier M H, Leroy P, Ganal M W. A microsatellite map of wheat [J]. Genetics, 1998, 149(4): 2007-2023
[6] Bernet G P, Bramardi S, Calvache D, Carbonell E A, Asins M J. Applicability of molecular markers in the context of protection of new varieties of cucumber [J]. Plant Breeding, 2003, 122(2): 146-152
[7] Tommasini L, Batley J, Arnold G M, Cooke R J, Donini P, Lee D, Law J R, Lowe C, Moule C, Trick M, Edwards K J. The development of multiplex simple sequence repeat (SSR) markers to complement distinctness, uniformity and stability testing of rape (Brassica napusL.) varieties[J]. Theoretical Applied Genetics, 2003, 106(6):1091-1101.<sub>
[8]</sub><sub>Singh R K</sub><sub>, Shar</sub><sub>ma R K</sub><sub>, Singh A K</sub><sub>,</sub> <sub>V</sub><sub>,</sub><sub> Singh</sub><sub>V P</sub><sub>,</sub><sub>Singh</sub><sub> N K, </sub><sub>Tiwari</sub><sub> S P</sub><sub>, </sub><sub>Mohapatra</sub><sub> T</sub><sub>. Suitability of mapped sequence tagged mic</sub><sub>rosatellite site markers for establishing distinctness, uniformity and stability in aromatic rice</sub><sub>[J]. Euphytica, 2004, 135(2):</sub><sub>135-143.</sub>
[9] 陈本银, 姜慧芳, 任小平, 廖伯寿, 黄家权. 野生花生抗青枯病种质的发掘及分子鉴定[J]. 华北农学报, 2008, 23(3): 170-175Chen B Y, Jiang H F, Ren X P, Liao B S, Huang J Q. Identification and molecular traits of Arachis species with resistance to bacterial wilt[J]. Acta Agriculture Boreali-Sinica, 2008, 23(3): 170-175
[10] 唐荣华, 庄伟建, 高国庆, 韩柱强, 钟瑞春, 贺梁琼, 周翠球. 珍珠豆型花生的简单序列重复(SSR)多态性[J]. 中国油料作物学报, 2004, 26: 20-26Tang R H, Zhuang W J, Gao G Q, Han Z Q, Zhong R C, He L Q, Zhou C Q. Simple sequence repeats polymorphism among accessions of var. Vulgaris Harz in Arachis hypogaea L [J]. Chinese Journal of Oil Crop Sciences, 2004, 26(2): 20-26
[11] 詹世雄, 刘冠明, 郑奕雄, 杨灵, 张平湖, 庄东红. 40个珍珠豆型花生SSR 指纹图谱的构建[J]. 种子, 2012, 31(06): 23-27Zhan S X, Liu G M, Zheng Y X, Yang L, Zhang P H, Zhuang D H. Construction of molecular fingerprinting for forty Spanish-type peanut using SSR markers [J]. Seed, 2012, 31(06): 23-27
[12] 姜慧芳, 任小平, 王圣玉, 黄家权, 雷永, 廖伯寿. 野生花生高油基因资源的发掘与鉴定[J]. 中国油料作物学报, 2010, 32(01): 30-34Jiang H F, Ren X P, Wang S Y, Huang J Q, Lei Y, Liao B S. Identification and evaluation of high oil contenting wild Arachis species [J]. Chinese Journal of Oil Crop Sciences, 2010, 32(01): 30-34
[13] 尹亮, 李双铃, 任艳, 石延茂, 袁美. 42个花生品种的SSR 标记指纹图谱构建[J]. 花生学报, 2017, 46(01): 8-13Yi L, Li S L, Ren Y, Shi Y M, Yuan M. Construction of molecular fingerprint for 42 peanut varieties using SSR markers [J]. Journal of Peanut Science, 2017, 46(01): 8-13
[14] 黄金堂, 陈海玲, 郑国栋, 李清华, 邱国清, 李淑萍, 谢志琼. 福建花生品种系谱及其性状演变分析[J]. 中国农学通报, 2012, 28(27): 31-36Huang J T, Chen H L, Zheng G D, Li Q H, Qiu G Q, LI S P, Xie Z Q. Analysis of pedigree and evolution of traits for peanut varieties registered in Fujian [J]. Chinese Agricultural Science Bulletin, 2012, 28(27): 31-36
[15] 陈永水, 陈剑洪, 郭陞垚, 肖宇, 陈茹艳, 王金线. 福建省审(认)定花生品种系谱及主要性状遗传改良分析[J]. 中国农学通报, 2014, 30(18): 136-144Chen Y S, Chen J H, Guo S Y, Xiao Y, Chen R Y, Wang J X. Analysis of pedigree and main traits improvement of peanut cultivars registered in Fujian [J]. Chinese Agricultural Science Bulletin, 2014, 30(18): 136-144
[16] 陈志德, 俞春涛, 谢吉先, 张祖明, 刘永惠. 江苏花生品种系谱分析及农艺性状的演变[J]. 花生学报, 2011, 40(02): 20-23Chen Z D, Yu C T, Xie J X, Zhang Z M, Liu Y H. Pedigree analysis and evolution of agronomic traits for peanut varieties registered in Jiangsu [J]. Journal of Peanut Science, 2011, 40(02): 20-23
[17] 于树涛, 于洪波, 苏君伟, 赵立仁, 史普想, 唐月异, 王秀贞, 吴琪. 辽宁花生品种系谱分析及农艺性状的演变[J]. 植物遗传资源学报, 2014, 15(02): 423-428Yu S T, Yu H B, Su J W, Zhao L R, Shi P X, Tang Y Y, Wang X Z, Wu Q. Pedigree analysis and evolution of agronomic traits for peanut varieties registered in Liaoning [J]. Journal of Plant Genetic Resources, 2014, 15(02): 423-428
[18] 孙晓苹, 陈小姝, 吕永超, 李春雨, 王绍伦, 刘海龙, 高华援. 吉林省花生品种系谱分析[J].东北农业科学, 2017, 42(06):23-27Sun X P. Chen X S, Lyu Y C, Li C Y, Wang S L, Liu H L, Gao H Y. Analysis on pedigree of peanut varieties registered in Jilin Province [J]. Journal of Jilin Agricultural Sciences. 2017,42(06): 23-27
[19] 沈一, 鄂志国, 刘永惠, 陈志德. 中国花生品种及其系谱数据库的构建[J]. 中国油料作物学报, 2015, 37(04): 571-575Shen Y, E Z G, Liu Y H, Chen Z D. Database construction of Chinese peanut varieties and their genealogy [J]. Chinese Journal of Oil Crop Sciences, 2015, 37(04): 571-575
[20] 禹山林. 中国花生品种及其系谱[M]. 上海 :上海科学技术出版社, 2008.1-54Yu S L. Peanut varieties and pedigree China [M]. Shanghai: Shanghai Scientific Technical Publishers, 2008. 1-54
[21] Dang P M, Chen C Y. Modified method for combined DNA and RNA isolation from peanut and other oil seeds [J]. Molecular Biology Reports, 2013, 40: 1563-1568
[22] 周金超. 花生遗传连锁图谱构建及农艺性状的QTL定位分析[D]. 河北农业大学, 河北保定, 2014Zhou J C. Genetic map construction and QTL mapping for agronomic traits in cultivated peanut (Arachis hypogaea L.) [D]. Hebei: Hebei Agricultural University, 2014<sub>
[23]</sub> <sub>Williams</sub><sub>J G</sub><sub>,</sub><sub>Kubelik </sub><sub>A R</sub><sub>,</sub><sub>Livak </sub><sub>K J,</sub><sub> Rafalski </sub><sub>J A,</sub><sub> Tingey</sub><sub> S V</sub><sub>.</sub><sub>DNA polymorphisms amplified by arbitrary primers are useful </sub><sub>as genetic markers</sub><sub> [J]</sub><sub>. </sub><sub>Nuc</sub><sub>le</sub><sub>ic </sub><sub>Acids Research</sub><sub>, 1990, 18</sub><sub>(22)</sub><sub>:</sub><sub> 6531-6534</sub>
[24] Botstein D, White R L, Skolnick M, Davis R W. Construction of a genetic linkage map in man using restriction fragment length polymorphisms [J]. American Journal of Human Genetics, 1980, 32(3): 314-314
[25] 谭正宝. 中国东北大豆育成品种(1923-2005)系谱和遗传多样性分析[D]. 南昌: 南昌大学, 2008Tan Z B. The pedigree and genetic diversity on soybean cultivars released during 1923-2005 in Northeast [D]. China Nanchang: Nanchang University, 2008
[26] 熊冬金. 中国大豆育成品种(1923-2005)基于系谱和SSR标记的遗传基础研究[D]. 南京: 南京农业大学, 2009Xiong D J. Studies on the genetic bases of Chinese soybean cultivars released during 1923-2005 based on by pedigree and SSR marker analyses [D]. Nanjing: Nanjing Agricultural University, 2009
[27] 刘文欣. 建国以来我国棉花品种遗传改良研究[D]. 北京: 中国农业大学, 2004Liu W X. Studies on genetic improvement in cotton planted in China since 1949 [D]. Beijing: China Agricultural University, 2004
[28] 高华援, 凤桐, 张宇航, 赵叶明, 王庆峰, 周玉萍, 王丽, 王喜山, 孙晓平.多粒型优质高产早熟花生新品种吉花3号的选育[J]. 花生学报, 2013, 42(03): 67-68Gao H Y, Feng T, Zhang Y H, Zhao Y M, Wang Q F, Zhou Y P, Wang L, Wang M S, Sun X P. Breeding of Jihua3, a Valencia type peanut cultivar with good quality, high yield and short duration [J]. Journal of Peanut Science, 2013, 42(03): 67-68
[29] 周彦忠, 姬小玲, 谢连芳, 郭玉生. 高产稳产抗病花生新品种漯花1号的选育[J]. 中国种业, 2012(02): 47-48Zhou Y Z, Ji X L, Xie L F, Guo Y S. Breeding of a new peanut cultivar Luohua1, high yield, disease resistance [J]. China Seed Industry, 2012(02): 47-48
[30] 杨余, 刘晓光, 范燕, 赵雪飞. 高产优质抗逆花生新品种唐花11号的选育[J]. 中国种业, 2011(07): 52-54Yang Y, Liu X G, Fan Yan, Zhao X F. Breeding of a new peanut cultivar Tanghua11, high yield, high quality, stable yield and resistance [J]. China Seed Industry, 2011(07): 52-54
[31] 苏江顺, 顾广霞, 刘伟, 张云万, 马凤华, 梁秀雪. 花生白院花2号的选育及栽培技术要点[C]. 吉林省科学技术协会, 2012: 2Su J S, Gu G X, Liu W, Zhang Y W, Ma F H, Liang X X. Breeding and cultivation techniques of peanut Bai yuan hua2 [C]. Science and Technology annual meeting in Jilin Province, 2012: 2
[32] 李玉荣, 程增书, 徐桂真, 王延兵. 高产高油花生新品种冀花4号的选育[C]. 中国作物学会油料作物专业委员会学术年会, 2004Li Y R, Cheng Z S, Xu G Z, Wang Y B. Breeding of a new peanut cultivar Ji hua4, high yield and high oil [C]. China crop society about oil crop specialized committee academic annual meeting, 2004
[33] 周彦忠, 姬小玲, 王振, 王璐, 刘平. 高产、抗病、优质花生新品种漯花6号的选育[J]. 河南农业科学, 2009(10): 79-80Zhou Y Z, Ji X L, Wang Z, Wang L, Liu P. Breeding of a new peanut cultivar Luohhua6, high yield, disease resistance, high quality [J]. Journal of Henan Agricultural Sciences, 2009(10), 10: 79-80
[34] 崔顺立, 陈焕英, 穆国俊, 杨鑫雷, 孟庆荣, 侯名语, 何美敬,李文平, 刘立峰. 花生品种冀农花1号的选育及配套栽培技术[J]. 中国种业, 2015(04): 61-62Cui S L, Chen H Y, Mu G J, Yang X L, Meng Q R, Hou M Y, He M J, Li W P, Liu L F. Breeding and supporting cultivation techniques of peanut cultivar, Jinonghua1. China Seed Industry, 2015(04): 61-62
[35] 崔顺立, 穆国俊, 杨鑫雷, 孟庆荣, 侯名语, 何美敬,李文平, 刘立峰. 适宜机收花生新品种冀农花2号的选育及配套栽培技术[J]. 种子, 2015, 34(10): 107-109Cui S L, Mu G J, Yang X L, Meng Q R, Hou M Y, He M J, Li W P, Liu L F. Breeding and matching cultivation techniques of Jinonghua2, a suitable for machines harvesting peanut new varieties [J]. Seed, 2015, 34(10): 107-109
[36] 程增书. 花生新品种冀花6号、冀花7号通过鉴定[J]. 花生学报, 2009, 38(01): 39Cheng Z S. Identification of new peanut cultivar Jihua6 and Jihua7 [J]. Journal of Peanut Science, 2009, 38(01): 39
[37] 杨余, 范燕, 赵雪飞. 花生新品种唐花10号的选育. 现代农业科技, 2010(23): 100-101Yang Y, Fan Y, Zhao X F. Breeding of a new peanut variety Tanghua10 [J]. Modern Agricultural Science and Technology, 2010(23): 100-101
[38] 杨余, 范燕, 赵雪飞. 优质花生新品种唐花9号的选育及应用前景分析[J]. 花生学报, 2011, 40(02): 37-39Yang Y, Fan Y, Zhao X F. Breeding and application prospect analysis of a new peanut variety Tanghua9 [J]. Journal of Peanut Science, 2011, 40(02): 37-39
[39] 齐丽雅, 臧殿高, 何孟霞, 肖家雄. 花生新品种邢花4号的选育[J]. 河北农业科学, 2006(01): 90-91Qi L Y, Zang D G, He M X, Xiao J X.Breeding of a new peanut cultivar Xinghua4 [J]. Journal of Hebei Agricultural Sciences. 2006(01): 90-91
[40] 齐丽雅, 何孟霞, 杨永强, 马明, 刘宇阳, 王莉. 花生新品种邢花6号的选育[J]. 花生学报, 2012, 41(02): 46-48Qi L Y, He M X, Yang Y Q, Ma M, Liu Y Y, Wang L. Breeding of a new peanut variety Xinghua6 [J]. Journal of Peanut Science. 2012, 41(02): 46-48
[41] 何孟霞, 齐丽雅, 马明, 刘红, 胡先安, 刘志锋. 花生新品种邢花7号的选育. 河北农业科学, 2013, 17(04): 56-58Heng M X, Qi L Y, Ma M, Liu H, Hu X A, Liu Z F. Breeding of new peanut variety Xinghua7 [J]. Journal of Hebei Agricultural Sciences, 2013, 17(04): 56-58
[42] 齐丽雅, 何孟霞, 陈永建, 郭志英, 杨永强, 刘宇阳. 花生新品种邢花5号的选育[J]. 河北农业科学, 2009, 13(06): 59-60Qi L Y, He M X, Chen Y J, Guo Z Y, Yang Y Q, Liu Y Y. Breeding of new peanut variety Xinghua5 [J]. Journal of Hebei Agricultural Sciences, 2009, 13(06): 59-60
[43] 董文召, 张新友, 黄冰艳, 张忠信, 汤丰收, 韩锁义, 徐静, 高伟. 河南、山东育成花生品种的亲本遗传贡献及相关育种策略探讨[J]. 河南农业科学, 2014, 43(12): 40-45Dong W Z, Zhang X Y, Huang B Y, Zhang Z X, Tang F S, Han S Y, X u J, Gao W. Genetic contribution of breeding parents to released peanut varieties in Henan and Shandong provinces and prospect of related breeding strategy [J]. Journal of Henan Agricultural Sciences, 2014, 43(12): 40-45
[44] 刘冠明, 郑奕雄, 黎国良. 20个花生品种的SSR标记指纹图谱构建[J]. 中国农学通报, 2006, 22(06): 49-51Liu G M, Zheng Y X, Li G L. SSR fingerprinting map of 20 peanut cultivars [J]. Chinese Agricultural Science Bulletin, 2006, 22(06): 49-51
[45] 王燕龙. 花生栽培种遗传多样性的SSR分析及其指纹图谱的构建[D]. 济宁:曲阜师范大学, 2013Wang Y L. SSR analysis of genetic diversity of peanut cultivation and the construction of fingerprints[D]. Jining: Qufu Normal University, 2013
PDF(14587 KB)

19

Accesses

0

Citation

Detail
段落导航
相关文章

/

〈 〉