[Objective] Currently, pig farming facilities mainly rely on manual counting for tracking slaughtered and stored pigs. This is not only time-consuming and labor-intensive, but also prone to counting errors due to pig movement and potential cheating. As breeding operations expand, the periodic live asset inventories put significant strain on human, material and financial resources. Although methods based on electronic ear tags can assist in pig counting, these ear tags are easy to break and fall off in group housing environments. Most of the existing methods for counting pigs based on computer vision require capturing images from a top-down perspective, necessitating the installation of cameras above each hogpen or even the use of drones, resulting in high installation and maintenance costs. To address the above challenges faced in the group pig counting task, a high-efficiency and low-cost pig counting method was proposed based on improved instance segmentation algorithm and WeChat public platform. [Methods] Firstly, a smartphone was used to collect pig image data in the area from a human view perspective, and each pig's outline in the image was annotated to establish a pig count dataset. The training set contains 606 images and the test set contains 65 images. Secondly, an efficient global attention module was proposed by improving convolutional block attention module (CBAM). The efficient global attention module first performed a dimension permutation operation on the input feature map to obtain the interaction between its channels and spatial dimensions. The permuted features were aggregated using global average pooling (GAP). One-dimensional convolution replaced the fully connected operation in CBAM, eliminating dimensionality reduction and significantly reducing the model's parameter number. This module was integrated into the YOLOv8 single-stage instance segmentation network to build the pig counting model YOLOv8x-Ours. By adding an efficient global attention module into each C2f layer of the YOLOv8 backbone network, the dimensional dependencies and feature information in the image could be extracted more effectively, thereby achieving high-accuracy pig counting. Lastly, with a focus on user experience and outreach, a pig counting WeChat mini program was developed based on the WeChat public platform and Django Web framework. The counting model was deployed to count pigs using images captured by smartphones. [Results and Discussions] Compared with existing methods of Mask R-CNN, YOLACT(Real-time Instance Segmentation), PolarMask, SOLO and YOLOv5x, the proposed pig counting model YOLOv8x-Ours exhibited superior performance in terms of accuracy and stability. Notably, YOLOv8x-Ours achieved the highest accuracy in counting, with errors of less than 2 and 3 pigs on the test set. Specifically, 93.8% of the total test images had counting errors of less than 3 pigs. Compared with the two-stage instance segmentation algorithm Mask R-CNN and the YOLOv8x model that applies the CBAM attention mechanism, YOLOv8x-Ours showed performance improvements of 7.6% and 3%, respectively. And due to the single-stage design and anchor-free architecture of the YOLOv8 model, the processing speed of a single image was only 64 ms, 1/8 of Mask R-CNN. By embedding the model into the WeChat mini program platform, pig counting was conducted using smartphone images. In cases where the model incorrectly detected pigs, users were given the option to click on the erroneous location in the result image to adjust the statistical outcomes, thereby enhancing the accuracy of pig counting. [Conclusions] The feasibility of deep learning technology in the task of pig counting was demonstrated. The proposed method eliminates the need for installing hardware equipment in the breeding area of the pig farm, enabling pig counting to be carried out effortlessly using just a smartphone. Users can promptly spot any errors in the counting results through image segmentation visualization and easily rectify any inaccuracies. This collaborative human-machine model not only reduces the need for extensive manpower but also guarantees the precision and user-friendliness of the counting outcomes.

[Objective] There exist a high genetic correlation among various morphological characteristics of Mongolian horses. Utilizing advanced technology to obtain body structure parameters related to athletic performance could provide data support for breeding institutions to develop scientific breeding plans and establish the groundwork for further improvement of Mongolian horse breeds. However, traditional manual measurement methods are time-consuming, labor-intensive, and may cause certain stress responses in horses. Therefore, ensuring precise and effective measurement of Mongolian horse body dimensions is crucial for formulating early breeding plans. [Method] Video images of 50 adult Mongolian horses in the suitable breeding stage at the Inner Mongolia Agricultural University Horse Breeding Technical Center was first collected. Fifty images per horse were captured to construct the training and validation sets, resulting in a total of 2 500 high-definition RGB images of Mongolian horses, with an equal ratio of images depicting horses in motion and at rest. To ensure the model's robustness and considering issues such as angles, lighting, and image blurring during actual image capture, a series of enhancement algorithms were applied to the original dataset, expanding the Mongolian horse image dataset to 4 000 images. The YOLOv8n-pose was employed as the foundational keypoint detection model. Through the design of the C2f_DCN module, deformable convolution (DCNV2) was integrated into the C2f module of the Backbone network to enhance the model's adaptability to different horse poses in real-world scenes. Besides, an SA attention module was added to the Neck network to improve the model's focus on critical features. The original loss function was replaced with SCYLLA-IoU (SIoU) to prioritize major image regions, and a cosine annealing method was employed to dynamically adjust the learning rate during model training. The improved model was named DSS-YOLO (DCNv2-SA-SIoU-YOLO) network model. Additionally, a test set comprising 30 RGB-D images of mature Mongolian horses was selected for constructing body dimension measurement tasks. DSS-YOLO was used for keypoint detection of body dimensions. The 2D keypoint coordinates from RGB images were fused with corresponding depth values from depth images to obtain 3D keypoint coordinates, and Mongolian horse's point cloud information was transformed. Point cloud processing and analysis were performed using pass-through filtering, random sample consensus (RANSAC) shape fitting, statistical outlier filtering, and principal component analysis (PCA) coordinate system correction. Finally, body height, body oblique length, croup height, chest circumference, and croup circumference were automatically computed based on keypoint spatial coordinates. [Results and Discussion] The proposed DSS-YOLO model exhibited parameter and computational costs of 3.48 M and 9.1 G, respectively, with an average accuracy mAP_0.5:0.95 reaching 92.5%, and a d_DSS of 7.2 pixels. Compared to Hourglass, HRNet, and SimCC, mAP_0.5:0.95 increased by 3.6%, 2.8%, and 1.6%, respectively. By relying on keypoint coordinates for automatic calculation of body dimensions and suggesting the use of a mobile least squares curve fitting method to complete the horse's hip point cloud, experiments involving 30 Mongolian horses showed a mean average error (MAE) of 3.77 cm and mean relative error (MRE) of 2.29% in automatic measurements. [Conclusions] The results of this study showed that DSS-YOLO model combined with three-dimensional point cloud processing methods can achieve automatic measurement of Mongolian horse body dimensions with high accuracy. The proposed measurement method can also be extended to different breeds of horses, providing technical support for horse breeding plans and possessing practical application value.

[Objective] The body size parameter of cattle is a key indicator reflecting the physical development of cattle, and is also a key factor in the cattle selection and breeding process. In order to solve the demand of measuring body size of beef cattle in the complex environment of large-scale beef cattle ranch, an image acquisition device and an automatic measurement algorithm of body size were designed. [Methods] Firstly, the walking channel of the beef cattle was established, and when the beef cattle entered the restraining device through the channel, the RGB and depth maps of the image on the right side of the beef cattle were acquired using the Inter RealSense D455 camera. Secondly, in order to avoid the influence of the complex environmental background, an improved instance segmentation network based on Mask2former was proposed, adding CBAM module and CA module, respectively, to improve the model's ability to extract key features from different perspectives, extracting the foreground contour from the 2D image of the cattle, partitioning the contour, and comparing it with other segmentation algorithms, and using curvature calculation and other mathematical methods to find the required body size measurement points. Thirdly, in the processing of 3D data, in order to solve the problem that the pixel point to be measured in the 2D RGB image was null when it was projected to the corresponding pixel coordinates in the depth-valued image, resulting in the inability to calculate the 3D coordinates of the point, a series of processing was performed on the point cloud data, and a suitable point cloud filtering and point cloud segmentation algorithm was selected to effectively retain the point cloud data of the region of the cattle's body to be measured, and then the depth map was 16. Then the depth map was filled with nulls in the field to retain the integrity of the point cloud in the cattle body region, so that the required measurement points could be found and the 2D data could be returned. Finally, an extraction algorithm was designed to combine 2D and 3D data to project the extracted 2D pixel points into a 3D point cloud, and the camera parameters were used to calculate the world coordinates of the projected points, thus automatically calculating the body measurements of the beef cattle. [Results and Discussions] Firstly, in the part of instance segmentation, compared with the classical Mask R-CNN and the recent instance segmentation networks PointRend and Queryinst, the improved network could extract higher precision and smoother foreground images of cattles in terms of segmentation accuracy and segmentation effect, no matter it was for the case of occlusion or for the case of multiple cattles. Secondly, in three-dimensional data processing, the method proposed in the study could effectively extract the three-dimensional data of the target area. Thirdly, the measurement error of body size was analysed, among the four body size measurement parameters, the smallest average relative error was the height of the cross section, which was due to the more prominent position of the cross section, and the different standing positions of the cattle have less influence on the position of the cross section, and the largest average relative error was the pipe circumference, which was due to the influence of the greater overlap of the two front legs, and the higher requirements for the standing position. Finally, automatic body measurements were carried out on 137 beef cattle in the ranch, and the automatic measurements of the four body measurements parameters were compared with the manual measurements, and the results showed that the average relative errors of body height, cross section height, body slant length, and tube girth were 4.32%, 3.71%, 5.58% and 6.25%, respectively, which met the needs of the ranch. The shortcomings were that fewer body-size parameters were measured, and the error of measuring circumference-type body-size parameters was relatively large. Later studies could use a multi-view approach to increase the number of body rule parameters to be measured and improve the accuracy of the parameters in the circumference category. [Conclusions] The article designed an automatic measurement method based on two-dimensional and three-dimensional contactless body measurements of beef cattle. Moreover, the innovatively proposed method of measuring tube girth has higher accuracy and better implementation compared with the current research on body measurements in beef cattle. The relative average errors of the four body tape parameters meet the needs of pasture measurements and provide theoretical and practical guidance for the automatic measurement of body tape in beef cattle.

[Objective] In the era of digital agriculture, real-time monitoring and predictive modeling of crop growth are paramount, especially in autonomous farming systems. Traditional crop growth models, often constrained by their reliance on static, rule-based methods, fail to capture the dynamic and multifactorial nature of vegetable crop growth. This research tried to address these challenges by leveraging the advanced reasoning capabilities of pre-trained large language models (LLMs) to simulate and predict vegetable crop growth with accuracy and reliability. Modeling the growth of vegetable crops within these platforms has historically been hindered by the complex interactions among biotic and abiotic factors. [Methods] The methodology was structured in several distinct phases. Initially, a comprehensive dataset was curated to include extensive information on vegetable crop growth cycles, environmental conditions, and management practices. This dataset incorporates continuous data streams such as soil moisture, nutrient levels, climate variables, pest occurrence, and historical growth records. By combining these data sources, the study ensured that the model was well-equipped to understand and infer the complex interdependencies inherent in crop growth processes. Then, advanced techniques was emploied for pre-training and fine-tuning LLMs to adapt them to the domain-specific requirements of vegetable crop modeling. A staged intelligent agent ensemble was designed to work within the digital twin platform, consisting of a central managerial agent and multiple stage-specific agents. The managerial agent was responsible for identifying transitions between distinct growth stages of the crops, while the stage-specific agents were tailored to handle the unique characteristics of each growth phase. This modular architecture enhanced the model's adaptability and precision, ensuring that each phase of growth received specialized attention and analysis. [Results and Discussions] The experimental validation of this method was conducted in a controlled agricultural setting at the Xiaotangshan Modern Agricultural Demonstration Park in Beijing. Cabbage (Zhonggan 21) was selected as the test crop due to its significance in agricultural production and the availability of comprehensive historical growth data. Over five years, the dataset collected included 4 300 detailed records, documenting parameters such as plant height, leaf count, soil conditions, irrigation schedules, fertilization practices, and pest management interventions. This dataset was used to train the LLM-based system and evaluate its performance using ten-fold cross-validation. The results of the experiments demonstrating the efficacy of the proposed system in addressing the complexities of vegetable crop growth modeling. The LLM-based model achieved 98% accuracy in predicting crop growth degrees and a 99.7% accuracy in identifying growth stages. These metrics significantly outperform traditional machine learning approaches, including long short-term memory (LSTM), XGBoost, and LightGBM models. The superior performance of the LLM-based system highlights its ability to reason over heterogeneous data inputs and make precise predictions, setting a new benchmark for crop modeling technologies. Beyond accuracy, the LLM-powered system also excels in its ability to simulate growth trajectories over extended periods, enabling farmers and agricultural managers to anticipate potential challenges and make proactive decisions. For example, by integrating real-time sensor data with historical patterns, the system can predict how changes in irrigation or fertilization practices will impact crop health and yield. This predictive capability is invaluable for optimizing resource allocation and mitigating risks associated with climate variability and pest outbreaks. [Conclusions] The study emphasizes the importance of high-quality data in achieving reliable and generalizable models. The comprehensive dataset used in this research not only captures the nuances of cabbage growth but also provides a blueprint for extending the model to other crops. In conclusion, this research demonstrates the transformative potential of combining large language models with digital twin technology for vegetable crop growth modeling. By addressing the limitations of traditional modeling approaches and harnessing the advanced reasoning capabilities of LLMs, the proposed system sets a new standard for precision agriculture. Several avenues also are proposed for future work, including expanding the dataset, refining the model architecture, and developing multi-crop and multi-region capabilities.

[Objective] The dynamic prediction of carbon emission from cold chain distribution is an important basis for the accurate assessment of carbon emission and its green credit grade. Facing the fact that the carbon emission of vehicles is affected by multiple factors, such as road condition information, driving characteristics, refrigeration parameters, etc., a dynamic prediction model of carbon emission was proposed from refrigerated vehicles that integrates multi-source information. [Methods] The backbone feature extraction network, neck feature fusion network and loss function of YOLOv8s was firstly improved. The full-dimensional dynamic convolution was introduced into the backbone feature extraction network, and the multidimensional attention mechanism was introduced to capture the contextual key information to improve the model feature extraction capability. A progressive feature pyramid network was introduced into the feature extraction network, which reduced the loss of key information by fusing features layer by layer and improved the feature fusion efficiency. The road condition information recognition model based on improved YOLOv8s was constructed to characterize the road condition information in terms of the number of road vehicles and the percentage of pixel area. Pearson's correlation coefficient was used to compare and analyze the correlation between carbon emissions of refrigerated vehicles and different influencing factors, and to verify the necessity and criticality of the selection of input parameters of the carbon emission prediction model. Then the iTransformer temporal prediction model was improved, and the external attention mechanism was introduced to enhance the feature extraction ability of iTransformer model and reduce the computational complexity. The dynamic prediction model of carbon emission of refrigerated vehicles based on the improved iTransformer was constructed by taking the road condition information, driving characteristics (speed, acceleration), cargo weight, and refrigeration parameters (temperature, power) as inputs. Finally, the model was compared and analyzed with other models to verify the robustness of the road condition information and the prediction accuracy of the vehicle carbon emission dynamic prediction model, respectively. [Results and Discussions] The results of correlation analysis showed that the vehicle driving parameters were the main factor affecting the intensity of vehicle carbon emissions, with a correlation of 0.841. The second factor was cargo weight, with a correlation of 0.807, which had a strong positive correlation. Compared with the vehicle refrigeration parameters, the road condition information had a stronger correlation between vehicle carbon emissions, the correlation between refrigeration parameters and the vehicle carbon emissions impact factor were above 0.67. In order to further ensure the accuracy of the vehicle carbon emissions prediction model, The paper was selected as the input parameters for the carbon emissions prediction model. The improved YOLOv8s road information recognition model achieved 98.1%, 95.5%, and 98.4% in precision, recall, and average recognition accuracy, which were 1.2%, 3.7%, and 0.2% higher than YOLOv8s, respectively, with the number of parameters and the amount of computation being reduced by 12.5% and 31.4%, and the speed of detection being increased by 5.4%. This was due to the cross-dimensional feature learning through full-dimensional dynamic convolution, which fully captured the key information and improved the feature extraction capability of the model, and through the progressive feature pyramid network after fusing the information between different classes through gradual step-by-step fusion, which fully retained the important feature information and improved the recognition accuracy of the model. The predictive performance of the improved iTransformer carbon emission prediction model was better than other time series prediction models, and its prediction curve was closest to the real carbon emission curve with the best fitting effect. The introduction of the external attention mechanism significantly improved the prediction accuracy, and its MSE, MAE, RMSE and R² were 0.026 1 %VOL, 0.079 1 %VOL, 0.161 5 %VOL and 0.940 0, respectively, which were 0.4%, 15.3%, 8.7% and 1.3% lower, respectively, when compared with iTransformer. As the degree of road congestion increased, the prediction accuracy of the constructed carbon emission prediction model increased. [Conclusions] The carbon emission prediction model for cold chain distribution under multi-source information fusion proposed in this study can realize accurate prediction of carbon emission from refrigerated vehicles, provide theoretical basis for rationally formulating carbon emission reduction strategies and promoting the development of low-carbon cold chain distribution.

[Objective] In order to summarize exemplary cases of high-quality development in regional smart agriculture and contribute strategies for the sustainable advancement of the national smart agriculture cause, the spatiotemporal characteristics and key driving factors of smart farms in the Yangtze River Economic Belt were studied. [Methods] Based on data from 11 provinces (municipalities) spanning the years 2014 to 2023, a comprehensive analysis was conducted on the spatio-temporal differentiation characteristics of smart farms in the Yangtze River Economic Belt using methods such as kernel density analysis, spatial auto-correlation analysis, and standard deviation ellipse. Including the overall spatial clustering characteristics, high-value or low-value clustering phenomena, centroid characteristics, and dynamic change trends. Subsequently, the geographic detector was employed to identify the key factors driving the spatio-temporal differentiation of smart farms and to discern the interactions between different factors. The analysis was conducted across seven dimensions: special fiscal support, industry dependence, human capital, urbanization, agricultural mechanization, internet infrastructure, and technological innovation. [Results and Discussions] Firstly, in terms of temporal characteristics, the number of smart farms in the Yangtze River Economic Belt steadily increased over the past decade. The year 2016 marked a significant turning point, after which the growth rate of smart farms had accelerated noticeably. The development of the upper, middle, and lower reaches exhibited both commonalities and disparities. Specifically, the lower sub-regions got a higher overall development level of smart farms, with a fluctuating upward growth rate; the middle sub-regions were at a moderate level, showing a fluctuating upward growth rate and relatively even provincial distribution; the upper sub-regions got a low development level, with a stable and slow growth rate, and an unbalanced provincial distribution. Secondly, in terms of spatial distribution, smart farms in the Yangtze River Economic Belt exhibited a dispersed agglomeration pattern. The results of global auto-correlation indicated that smart farms in the Yangtze River Economic Belt tended to be randomly distributed. The results of local auto-correlation showed that the predominant patterns of agglomeration were H-L and L-H types, with the distribution across provinces being somewhat complex; H-H type agglomeration areas were mainly concentrated in Sichuan, Hubei, and Anhui; L-L type agglomeration areas were primarily in Yunnan and Guizhou. The standard deviation ellipse results revealed that the mean center of smart farms in the Yangtze River Economic Belt had shifted from Anqing city in Anhui province in 2014 to Jingzhou city in Hubei province in 2023, with the spatial distribution showing an overall trend of shifting southwestward and a slow expansion toward the northeast and south. Finally, in terms of key driving factors, technological innovation was the primary critical factor driving the formation of the spatio-temporal distribution pattern of smart farms in the Yangtze River Economic Belt, with a factor explanatory degree of 0.311 1. Moreover, after interacting with other indicators, it continued to play a crucial role in the spatio-temporal distribution of smart farms, which aligned with the practical logic of smart farm development. Urbanization and agricultural mechanization levels were the second and third largest key factors, with factor explanatory degrees of 0.292 2 and 0.251 4, respectively. The key driving factors for the spatio-temporal differentiation of smart farms in the upper, middle, and lower sub-regions exhibited both commonalities and differences. Specifically, the top two key factors driver identification in the upper region were technological innovation (0.841 9) and special fiscal support (0.782 3). In the middle region, they were technological innovation (0.619 0) and human capital (0.600 1), while in the lower region, they were urbanization (0.727 6) and technological innovation (0.425 4). The identification of key driving factors and the detection of their interactive effects further confirmed that the spatio-temporal distribution characteristics of smart farms in the Yangtze River Economic Belt were the result of the comprehensive action of multiple factors. [Conclusions] The development of smart farms in the Yangtze River Economic Belt is showing a positive momentum, with both the total number of smart farms and the number of sub-regions experiencing stable growth. The development speed and level of smart farms in the sub-regions exhibit a differentiated characteristic of "lower reaches > middle reaches > upper reaches". At the same time, the overall distribution of smart farms in the Yangtze River Economic Belt is relatively balanced, with the degree of sub-regional distribution balance being "middle reaches (Hubei province, Hunan province, Jiangxi province are balanced) > lower reaches (dominated by Anhui) > upper reaches (Sichuan stands out)". The coverage of smart farm site selection continues to expand, forming a "northeast-southwest" horizontal diffusion pattern. In addition, the spatio-temporal characteristics of smart farms in the Yangtze River Economic Belt are the result of the comprehensive action of multiple factors, with the explanatory power of factors ranked from high to low as follows: Technological innovation > urbanization > agricultural mechanization > human capital > internet infrastructure > industry dependence > special fiscal support. Moreover, the influence of each factor is further strengthened after interaction. Based on these conclusions, suggestions are proposed to promote the high-quality development of smart farms in the Yangtze River Economic Belt. This study not only provides a theoretical basis and reference for the construction of smart farms in the Yangtze River Economic Belt and other regions, but also helps to grasp the current status and future trends of smart farm development.

[Objective] Traditional object detection algorithms applied in the agricultural field, such as those used for crop growth monitoring and harvesting, often suffer from insufficient accuracy. This is particularly problematic for small crops like mushrooms, where recognition and detection are more challenging. The introduction of small object detection technology promises to address these issues, potentially enhancing the precision, efficiency, and economic benefits of agricultural production management. However, achieving high accuracy in small object detection has remained a significant challenge, especially when dealing with varying image sizes and target scales. Although the YOLO series models excel in speed and large object detection, they still have shortcomings in small object detection. To address the issue of maintaining high accuracy amid changes in image size and target scale, a novel detection model, Multi-Strategy Handling YOLOv8 (MSH-YOLOv8), was proposed. [Methods] The proposed MSH-YOLOv8 model builds upon YOLOv8 by incorporating several key enhancements aimed at improving sensitivity to small-scale targets and overall detection performance. Firstly, an additional detection head was added to increase the model's sensitivity to small objects. To address computational redundancy and improve feature extraction, the Swin Transformer detection structure was introduced into the input module of the head network, creating what was termed the "Swin Head (SH)". Moreover, the model integrated the C2f_Deformable convolutionv4 (C2f_DCNv4) structure, which included deformable convolutions, and the Swin Transformer encoder structure, termed "Swinstage", to reconstruct the YOLOv8 backbone network. This optimization enhanced feature propagation and extraction capabilities, increasing the network's ability to handle targets with significant scale variations. Additionally, the normalization-based attention module (NAM) was employed to improve performance without compromising detection speed or computational complexity. To further enhance training efficacy and convergence speed, the original loss function CIoU was replaced with wise-intersection over union (WIoU) Loss. Furthermore, experiments were conducted using mushrooms as the research subject on the open Fungi dataset. Approximately 200 images with resolution sizes around 600×800 were selected as the main research material, along with 50 images each with resolution sizes around 200×400 and 1 000×1 200 to ensure representativeness and generalization of image sizes. During the data augmentation phase, a generative adversarial network (GAN) was utilized for resolution reconstruction of low-resolution images, thereby preserving semantic quality as much as possible. In the post-processing phase, dynamic resolution training, multi-scale testing, soft non-maximum suppression (Soft-NMS), and weighted boxes fusion (WBF) were applied to enhance the model's small object detection capabilities under varying scales. [Results and Discussions] The improved MSH-YOLOv8 achieved an average precision at 50% (AP50) intersection over union of 98.49% and an AP@50-95 of 75.29%, with the small object detection metric APs reaching 39.73%. Compared to mainstream models like YOLOv8, these metrics showed improvements of 2.34%, 4.06% and 8.55%, respectively. When compared to the advanced TPH-YOLOv5 model, the improvements were 2.14%, 2.76% and 6.89%, respectively. The ensemble model, MSH-YOLOv8-ensemble, showed even more significant improvements, with AP50 and APs reaching 99.14% and 40.59%, respectively, an increase of 4.06% and 8.55% over YOLOv8. These results indicate the robustness and enhanced performance of the MSH-YOLOv8 model, particularly in detecting small objects under varying conditions. Further application of this methodology on the Alibaba Cloud Tianchi databases "Tomato Detection" and "Apple Detection" yielded MSH-YOLOv8-t and MSH-YOLOv8-a models (collectively referred to as MSH-YOLOv8). Visual comparison of detection results demonstrated that MSH-YOLOv8 significantly improved the recognition of dense and blurry small-scale tomatoes and apples. This indicated that the MSH-YOLOv8 method possesses strong cross-dataset generalization capability and effectively recognizes small-scale targets. In addition to quantitative improvements, qualitative assessments showed that the MSH-YOLOv8 model could handle complex scenarios involving occlusions, varying lighting conditions, and different growth stages of the crops. This demonstrates the practical applicability of the model in real-world agricultural settings, where such challenges are common. [Conclusions] The MSH-YOLOv8 improvement method proposed in this study effectively enhances the detection accuracy of small mushroom targets under varying image sizes and target scales. This approach leverages multiple strategies to optimize both the architecture and the training process, resulting in a robust model capable of high-precision small object detection. The methodology's application to other datasets, such as those for tomato and apple detection, further underscores its generalizability and potential for broader use in agricultural monitoring and management tasks.

[Objective] In the retail market for agricultural products, consumers are increasingly concerned about the safety and health aspects of those products. Traceability of blockchain has emerged as a crucial solution to address these concerns. Essentially, a blockchain functions as a dynamic, distributed, and shared database. When implemented in the agricultural supply chain, it not only improves product transparency to attract more consumers but also raises concerns about consumer privacy disclosure. The level of consumer apprehension regarding privacy will directly influence their choice to purchase agricultural products traced through blockchain-traced. Moreover, retailers' choices to sell blockchain-traced produce are influenced by consumer privacy concerns. By analyzing the impact of blockchain technology on the competitive strategies, pricing, and decision-making among agricultural retailers, they can develop market competition strategies that suit their market conditions to bolster their competitiveness and optimize the agricultural supply chain to maximize overall benefits. [Methods] Based on Nash equilibrium and Stackelberg game theory, a market competition model was developed to analyze the interactions between existing and new agricultural product retailers. The competitive strategies adopted by agricultural product retailers were analyzed under four different options of whether two agricultural retailers sell blockchain agricultural products. It delved into product utility, optimal pricing, demand, and profitability for each retailer under these different scenarios. How consumer privacy concerns impact pricing and profits of two agricultural product retailers and the optimal response strategy choice of another retailer when the competitor made the decision choice first were also analyzed. This analysis aimed to guide agricultural product retailers in making strategic choices that would safeguard their profits and market positions. To address the cooperative game problem of agricultural product retailers in market competition, ensure that retailers could better cooperate in the game, blockchain smart contract technology was used. By encoding the process and outcomes of the Stackelberg game into smart contracts, retailers could input their specific variables and receive tailored strategy recommendations. Uploading game results onto the blockchain network ensured transparency and encouraged cooperative behavior among retailers. By using the characteristics of blockchain, the game results were uploaded to the blockchain network to regulate the cooperative behavior, to ensure the maximization of the overall interests of the supply chain. [Results and Discussions] The research highlighted the significant improvement in agricultural product quality transparency through blockchain traceability technology. However, concerns regarding consumer privacy arising from this traceability could directly impact the pricing, profitability and retailers' decisions to provide blockchain-traceable items. Furthermore, an analysis of the strategic balance between two agricultural product retailers revealed that in situations of low and high product information transparency, both retailers were inclined to simultaneously offer sell traceable products. In such a scenario, blockchain traceability technology enhanced the utility and profitability of retail agricultural products, leading consumers to prefer purchase these traceable products from retailers. In cases where privacy concerns and agricultural product information transparency were both moderate, the initial retailer was more likely to opt for blockchain-based traceable products. This was because consumers had higher trust in the initial retailer, enabling them to bear a higher cost associated with privacy concerns. Conversely, new retailers failed to gain a competitive advantage and eventually exit the market. When consumer privacy concerns exceeded a certain threshold, both competing agricultural retailers discovered that offering blockchain-based traceable products led to a decline in their profits. [Conclusions] When it comes to agricultural product quality and safety, incorporating blockchain technology in traceability significantly improves the transparency of quality-related information for agricultural products. However, it is important to recognize that the application of blockchain for agricultural product traceability is not universally suitable for all agricultural retailers. Retailers must evaluate their unique circumstances and make the most suitable decisions to enhance the effectiveness of agricultural products, drive sales demand, and increase profits. Within the competitive landscape of the agricultural product retail market, nurturing a positive collaborative relationship is essential to maximize mutual benefits and optimize the overall profitability of the agricultural product supply chain.

Objective The accuracy of identifying litchi pests is crucial for implementing effective control strategies and promoting sustainable agricultural development. However, the current detection of litchi pests is characterized by a high percentage of small targets, which makes target detection models challenging in terms of accuracy and parameter count, thus limiting their application in real-world production environments. To improve the identification efficiency of litchi pests, a lightweight target detection model YOLO-LP (YOLO-Litchi Pests) based on YOLOv10n was proposed. The model aimed to enhance the detection accuracy of small litchi pest targets in multiple scenarios by optimizing the network structure and loss function, while also reducing the number of parameters and computational costs. Methods Two classes of litchi insect pests (Cocoon and Gall) images were collected as datasets for modeling in natural scenarios (sunny, cloudy, post-rain) and laboratory environments. The original data were expanded through random scaling, random panning, random brightness adjustments, random contrast variations, and Gaussian blurring to balance the category samples and enhance the robustness of the model, generating a richer dataset named the CG dataset (Cocoon and Gall dataset). The YOLO-LP model was constructed after the following three improvements. Specifically, the C2f module of the backbone network (Backbone) in YOLOv10n was optimized and the C2f_GLSA module was constructed using the global-to-local spatial aggregation (GLSA) module to focus on small targets and enhance the differentiation between the targets and the backgrounds, while simultaneously reducing the number of parameters and computation. A frequency-aware feature fusion module (FreqFusion) was introduced into the neck network (Neck) of YOLOv10n and a frequency-aware path aggregation network (FreqPANet) was designed to reduce the complexity of the model and address the problem of fuzzy and shifted target boundaries. The SCYLLA-IoU (SIoU) loss function replaced the Complete-IoU (CIoU) loss function from the baseline model to optimize the target localization accuracy and accelerate the convergence of the training process. Results and Discussions YOLO-LP achieved 90.9%, 62.2%, and 59.5% for AP₅₀, AP_50:95, and AP-Small_50:95 in the CG dataset, respectively, and 1.9%, 1.0%, and 1.2% higher than the baseline model. The number of parameters and the computational costs were reduced by 13% and 17%, respectively. These results suggested that YOLO-LP had a high accuracy and lightweight design. Comparison experiments with different attention mechanisms validated the effectiveness of the GLSA module. After the GLSA module was added to the baseline model, AP₅₀, AP_50:95, and AP-Small_50:95 achieved the highest performance in the CG dataset, reaching 90.4%, 62.0%, and 59.5%, respectively. Experiment results comparing different loss functions showed that the SIoU loss function provided better fitting and convergence speed in the CG dataset. Ablation test results revealed that the validity of each model improvement and the detection performance of any combination of the three improvements was significantly better than the baseline model in the YOLO-LP model. The performance of the models was optimal when all three improvements were applied simultaneously. Compared to several mainstream models, YOLO-LP exhibited the best overall performance, with a model size of only 5.1 MB, 1.97 million parameters (Params), and a computational volume of 5.4 GFLOPs. Compared to the baseline model, the detection of the YOLO-LP performance was significantly improved across four multiple scenarios. In the sunny day scenario, AP₅₀, AP_50:95, and AP-Small_50:95 increased by 1.9%, 1.0 %, and 2.0 %, respectively. In the cloudy day scenario, AP₅₀, AP_50:95, and AP-Small_50:95 increased by 2.5%, 1.3%, and 1.3%, respectively. In the post-rain scenario, AP₅₀, AP_50:95, and AP-Small_50:95 increased by 2.0%, 2.4%, and 2.4%, respectively. In the laboratory scenario, only AP₅₀ increased by 0.7% over the baseline model. These findings indicated that YOLO-LP achieved higher accuracy and robustness in multi-scenario small target detection of litchi pests. Conclusions The proposed YOLO-LP model could improve detection accuracy and effectively reduce the number of parameters and computational costs. It performed well in small target detection of litchi pests and demonstrated strong robustness across different scenarios. These improvements made the model more suitable for deployment on resource-constrained mobile and edge devices. The model provided a valuable technical reference for small target detection of litchi pests in various scenarios.

[Objective] Manual disinfection in large-scale sheep farm is laborious, time-consuming, and often results in incomplete coverage and inadequate disinfection. With the rapid development of the application of artificial intelligence and automation technology, the automatic navigation and spraying robot for livestock and poultry breeding, has become a research hotspot. To maintain shed hygiene and ensure sheep health, an automatic navigation and spraying robot was proposed for sheep sheds. [Methods] The automatic navigation and spraying robot was designed with a focus on three aspects: hardware, semantic segmentation model, and control algorithm. In terms of hardware, it consisted of a tracked chassis, cameras, and a collapsible spraying device. For the semantic segmentation model, enhancements were made to the lightweight semantic segmentation model ENet, including the addition of residual structures to prevent network degradation and the incorporation of a squeeze-and-excitation network (SENet) attention mechanism in the initialization module. This helped to capture global features when feature map resolution was high, addressing precision issues. The original 6-layer ENet network was reduced to 5 layers to balance the encoder and decoder. Drawing inspiration from dilated spatial pyramid pooling, a context convolution module (CCM) was introduced to improve scene understanding. A criss-cross attention (CCA) mechanism was adapted to acquire context global features of different scales without cascading, reducing information loss. This led to the development of a double attention enet (DAENet) semantic segmentation model was proposed to achieve real-time and accurate segmentation of sheep shed surfaces. Regarding control algorithms, a method was devised to address the robot's difficulty in controlling its direction at junctions. Lane recognition and lane center point identification algorithms were proposed to identify and mark navigation points during the robot's movement outside the sheep shed by simulating real roads. Two cameras were employed, and a camera switching algorithm was developed to enable seamless switching between them while also controlling the spraying device. Additionally, a novel offset and velocity calculation algorithm was proposed to control the speeds of the robot's left and right tracks, enabling control over the robot's movement, stopping, and turning. [Results and Discussions] The DAENet model achieved a mean intersection over union (mIoU) of 0.945 3 in image segmentation tasks, meeting the required segmentation accuracy. During testing of the camera switching algorithm, it was observed that the time taken for the complete transition from camera to spraying device action does not exceed 15 seconds when road conditions changed. Testing of the center point and offset calculation algorithm revealed that, when processing multiple frames of video streams, the algorithm averages 0.04 to 0.055 per frame, achieving frame rates of 20 to 24 frames per second, meeting real-time operational requirements. In field experiments conducted in sheep farm, the robot successfully completed automatic navigation and spraying tasks in two sheds without colliding with roadside troughs. The deviation from the road and lane centerlines did not exceed 0.3 meters. Operating at a travel speed of 0.2 m/s, the liquid in the medicine tank was adequate to complete the spraying tasks for two sheds. Additionally, the time taken for the complete transition from camera to spraying device action did not exceed 15 when road conditions changed. The robot maintained an average frame rate of 22.4 frames per second during operation, meeting the experimental requirements for accurate and real-time information processing. Observation indicated that the spraying coverage rate of the robot exceeds 90%, meeting the experimental coverage requirements. [Conclusions] The proposed automatic navigation and spraying robot, based on the DAENet semantic segmentation model and center point recognition algorithm, combined with hardware design and control algorithms, achieves comprehensive disinfection within sheep sheds while ensuring safety and real-time operation.

[Objective] Acurately determining the suitable sowing date for winter wheat is of great significance for improving wheat yield and ensuring national food security. Traditional visual interpretation method is not only time-consuming and labor-intensive, but also covers a relatively small area. Remote sensing monitoring, belongs to post-event monitoring, exhibits a time lag. The aim of this research is to use the temperature threshold method and accumulated thermal time requirements for wheat leaves appearance method to analyze the suitable sowing date for winter wheat in county-level towns under the influence of long-term sequence of climate warming. [Methods] The research area were various townships in Qihe county, Shandong province. Based on European centre for medium-range weather forecasts (ECMWF) reanalysis data from 1997 to 2022, 16 meteorological data grid points in Qihe county were selected. Firstly, the bilinear interpolation method was used to interpolate the temperature data of grid points into the approximate center points of each township in Qihe county, and the daily average temperatures for each township were obtained. Then, temperature threshold method was used to determine the final dates of stable passage through 18, 16, 14 and 0 ℃. Key sowing date indicators such as suitable sowing temperature for different wheat varieties, growing degree days (GDD)≥0 ℃ from different sowing dates to before overwintering, and daily average temperature over the years were used for statistical analysis of the suitable sowing date for winter wheat. Secondly, the accumulated thermal time requirements for wheat leaves appearance method was used to calculate the appropriate date of GDD for strong seedlings before winter by moving forward from the stable date of dropping to 0 ℃. Accumulating the daily average temperatures above 0 ℃ to the date when the GDD above 0 ℃ was required for the formation of strong seedlings of wheat, a range of ±3 days around this calculated date was considered the theoretical suitable sowing date. Finally, combined with actual production practices, the appropriate sowing date of winter wheat in various townships of Qihe county was determined under the trend of climate warming. [Results and Discussions] The results showed that, from November 1997 to early December 2022, winter and annual average temperatures in Qihe county had all shown an upward trend, and there was indeed a clear trend of climate warming in various townships of Qihe county. Judging from the daily average temperature over the years, the temperature fluctuation range in November was the largest in a year, with a maximum standard deviation was 2.61 ℃. This suggested a higher likelihood of extreme weather conditions in November. Therefore, it was necessary to take corresponding measures to prevent and reduce disasters in advance to avoid affecting the growth and development of wheat. In extreme weather conditions, it was limited to determine the sowing date only by temperature or GDD. In cold winter years, it was too one-sided to consider only from the perspective of GDD. It was necessary to expand the range of GDD required for winter wheat before overwintering based on temperature changes to ensure the normal growth and development of winter wheat. The suitable sowing date for semi winter wheat obtained by temperature threshold method was from October 4th to October 16th, and the suitable sowing date for winter wheat was from September 27th to October 4th. Taking into account the GDD required for the formation of strong seedlings before winter, the suitable sowing date for winter wheat was from October 3rd to October 13th, and the suitable sowing date for semi winter wheat was from October 15th to October 24th, which was consisted with the suitable sowing date for winter wheat determined by the accumulated thermal time requirements for wheat leaves appearance method. Considering the winter wheat varieties planted in Qihe county, the optimal sowing date for winter wheat in Qihe county was from October 3rd to October 16th, and the optimal sowing date was from October 5th to October 13th. With the gradual warming of the climate, the suitable sowing date for wheat in various townships of Qihe county in 2022 was later than that in 2002. However, the sowing date for winter wheat was still influenced by factors such as soil moisture, topography, and seeding quality. The suitable sowing date for a specific year still needed to be adjusted to local conditions and flexibly sown based on the specific situation of that year. [Conclusions] The experimental results proved the feasibility of the temperature threshold method and accumulated thermal time requirements for wheat leaves appearance method in determining the suitable sowing date for winter wheat. The temperature trend can be used to identify cold or warm winters, and the sowing date can be adjusted in a timely manner to enhance wheat yield and reduce the impact of excessively high or low temperatures on winter wheat. The research results can not only provide decision-making reference for winter wheat yield assessment in Qihe county, but also provide an important theoretical basis for scientifically arrangement of agricultural production.

[Objective] Rice plays a crucial role in daily diet. The rice industry involves numerous links, from paddy planting to the consumer's table, and the integrity of the quality control data chain directly affects the credibility of rice quality control and traceability information. The process of rice traceability also faces security issues, such as the leakage of privacy information, which need immediate solutions. Additionally, the previous practice of uploading all information onto the blockchain leads to high storage costs and low system efficiency. To address these problems, this study proposed a differential privacy-enhanced blockchain-based quality control model for rice, providing new ideas and solutions to optimize the traditional quality regulation and traceability system. [Methods] By exploring technologies of blockchain, interplanetary file system (IPFS), and incorporating differential privacy techniques, a blockchain-based quality control model for rice with differential privacy enhancement was constructed. Firstly, the data transmission process was designed to cover the whole industry chain of rice, including cultivation, acquisition, processing, warehousing, and sales. Each module stored the relevant data and a unique number from the previous link, forming a reliable information chain and ensuring the continuity of the data chain for quality control. Secondly, to address the issue of large data volume and low efficiency of blockchain storage, the key quality control data of each link in the rice industry chain was stored in the IPFS. Subsequently, the hash value of the stored data was returned and recorded on the blockchain. Lastly, to enhance the traceability of the quality control model information, the sensitive information in the key quality control data related to the cultivation process was presented to users after undergoing differential privacy processing. Individual data was obfuscated to increase the credibility of the quality control information while also protecting the privacy of farmers' cultivation practices. Based on this model, a differential privacy-enhanced blockchain-based quality control system for rice was designed. [Results and Discussions] The architecture of the differential privacy-enhanced blockchain-based quality control system for rice consisted of the physical layer, transport layer, storage layer, service layer, and application layer. The physical layer included sensor devices and network infrastructure, ensuring data collection from all links of the industry chain. The transport layer handled data transmission and communication, securely uploading collected data to the cloud. The storage layer utilized a combination of traditional databases, IPFS, and blockchain to efficiently store and manage key data on and off the blockchain. The traditional database was used for the management and querying of structured data. IPFS stored the key quality control data in the whole industry chain, while blockchain was employed to store the hash values returned by IPFS. This integrated storage method improved system efficiency, ensured the continuity, reliability, and traceability of quality control data, and provided consumers with reliable information. The service layer was primarily responsible for handling business logic and providing functional services. The implementation of functions in the application layer relied heavily on the design of a series of interfaces within the service layer. Positioned at the top of the system architecture, the application layer was responsible for providing user-centric functionality and interfaces. This encompassed a range of applications such as web applications and mobile applications, aiming to present data and facilitate interactive features to fulfill the requirements of both consumers and businesses. Based on the conducted tests, the average time required for storing data in a single link of the whole industry chain within the system was 1.125 s. The average time consumed for information traceability query was recorded as 0.691 s. Compared to conventional rice quality regulation and traceability systems, the proposed system demonstrated a reduction of 6.64% in the storage time of single-link data and a decrease of 16.44% in the time required to perform information traceability query. [Conclusions] This study proposes a differential privacy-enhanced blockchain-based quality control model for rice. The model ensures the continuity of the quality control data chain by integrating the various links of the whole industry chain of rice. By combining blockchain with IPFS storage, the model addresses the challenges of large data volume and low efficiency of blockchain storage in traditional systems. Furthermore, the model incorporates differential privacy protection to enhance traceability while safeguarding the privacy of individual farmers. This study can provide reference for the design and improvement of rice quality regulation and traceability systems.

[Objective] Sunlight-induced chlorophyll fluorescence (SIF) data obtained from satellites suffer from issues such as low spatial and temporal resolution, and discrete footprint because of the limitations imposed by satellite orbits. To address these problems, obtaining higher resolution SIF data, most reconstruction studies are based on low-resolution satellite SIF. Moreover, the spatial resolution of most SIF reconstruction products is still not enough to be directly used for the study of crop photosynthetic rate at the regional scale. Although some SIF products boast elevated resolutions, but these derive not from the original satellite SIF data reconstruct but instead evolve from secondary reconstructions based on preexisting SIF reconstruction products. Satellite OCO-2 (The Orbiting Carbon Obsevatory-2) equipped with a high-resolution spectrometer, OCO-2 SIF has higher spatial resolution (1.29×2.25 km) compared to other original SIF products, making it suitable in advancing the realm of high-resolution SIF data reconstruction, particularly within the context of regional-scale crop studies. [Methods] This research primarily exploration SIF reconstruct at the regional scale, mainly focused on the partial soybean planting regions nestled within the United States. The selection of MODIS raw data hinged on a meticulous consideration of environmental conditions, the distinctive physiological attributes of soybeans, and an exhaustive evaluation of factors intricately linked to OCO-2 SIF within these soybean planting regions. The primary tasks of this research encompassed reconstructing high resolution soybean SIF while concurrently executing a rigorous assessment of the reconstructed SIF's quality. During the dataset construction process, amalgamated SIF data from multiple soybean planting regions traversed by the OCO-2 satellite's footprint to retain as many of the available original SIF samples as possible. This approach provided the subsequent SIF reconstruction model with a rich source of SIF data. SIF data obtained beneath the satellite's trajectory were matched with various MODIS datasets, including enhanced vegetation index (EVI), fraction of photosynthetically active radiation (FPAR), and land surface temperature (LST), resulting in the creation of a multisource remote sensing dataset ultimately used for model training. Because of the multisource remote sensing dataset encompassed the most relevant explanatory variables within each SIF footprint coverage area concerning soybean physiological structure and environmental conditions. Through the activation functions in the BP neural network, it enhanced the understanding of the complex nonlinear relationships between the original SIF data and these MODIS products. Leveraging these inherent nonlinear relationships, compared and analyzed the effects of different combinations of explanatory variables on SIF reconstruction, mainly analyzing the three indicators of goodness of fit R², root mean square error RMSE, and mean absolute error MAE, and then selecting the best SIF reconstruction model, generate a regional scale, spatially continuous, and high temporal resolution (500 m, 8 d) soybean SIF reconstruction dataset (BPSIF). [Results and Discussions] The research findings confirmed the strong performance of the SIF reconstruction model in predicting soybean SIF. After simultaneously incorporating EVI, FPAR, and LST as explanatory variables to model, achieved a goodness of fit with an R² value of 0.84, this statistical metric validated the model's capability in predicting SIF data, it also reflected that the reconstructed 8 d time resolution of SIF data's reliability of applying to small-scale agricultural crop photosynthesis research with 500 m×500 m spatial scale. Based on this optimal model, generated the reconstructed SIF product (BPSIF). The Pearson correlation coefficient between the original OCO-2 SIF data and MODIS GPP stood were at a modest 0.53. In stark contrast, the correlation coefficient between BPSIF and MODIS Gross Primary Productivity (GPP) rosed significantly to 0.80. The increased correlation suggests that BPSIF could more accurately reflect the dynamic changes in GPP during the soybean growing season, making it more reliable compared to the original SIF data. Selected soybean planting areas in the United States with relatively single crop cultivation as the research area, based on high spatial resolution (1.29 km×2.25 km) OCO-2 SIF data, greatly reduced vegetation heterogeneity under a single SIF footprint. [Conclusions] The BPSIF proposed has significantly enhancing the regional and temporal continuity of OCO-2 SIF while preserving the time and spatial attributes contained in the original SIF dataset. Within the study area, BPSIF exhibits a significantly improved correlation with MODIS GPP compared to the original OCO-2 SIF. The proposed OCO-2 SIF data reconstruction method in this study holds the potential to provide a more reliable SIF dataset. This dataset has the potential to drive further understanding of soybean SIF at finer spatial and temporal scales, as well as find its relationship with soybean GPP.

[Objective] Plug tray seedling cultivation is a contemporary method known for its high germination rates, uniform seedling growth, shortened transplant recovery period, diminished pest and disease incidence, and enhanced labor efficiency. Despite these advantages, challenges such as missing or underdeveloped seedlings can arise due to seedling quality and environmental factors. To ensure uniformity and consistency of the seedlings, sorting is frequently necessary, and the adoption of automated seedling sorting technology can significantly reduce labor costs. Nevertheless, the overgrowth of seedlings within the plugs can effect the accuracy of detection algorithms. A method for grading and locating strawberry seedlings based on a lightweight YOLOv8s model was presented in this research to effectively mitigate the interference caused by overgrown seedlings. [Methods] The YOLOv8s model was selected as the baseline for detecting different categories of seedlings in the strawberry plug tray cultivation process, namely weak seedlings, normal seedlings, and plug holes. To improve the detection efficiency and reduce the model's computational cost, the layer-adaptive magnitude-based pruning(LAMP) score-based channel pruning algorithm was applied to compress the base YOLOv8s model. The pruning procedure involved using the dependency graph to derive the group matrices, followed by normalizing the group importance scores using the LAMP Score, and ultimately pruning the channels according to these processed scores. This pruning strategy effectively reduced the number of model parameters and the overall size of the model, thereby significantly enhancing its inference speed while maintaining the capability to accurately detect both seedlings and plug holes. Furthermore, a two-stage seedling-hole matching algorithm was introduced based on the pruned YOLOv8s model. In the first stage, seedling and plug hole bounding boxes were matched according to their the degree of overlap (Dp), resulting in an initial set of high-quality matches. This step helped minimize the number of potential matching holes for seedlings exhibiting overgrowth. Subsequently, before the second stage of matching, the remaining unmatched seedlings were ranked according to their potential matching hole scores (S), with higher scores indicating fewer potential matching holes. The seedlings were then prioritized during the second round of matching based on these scores, thus ensuring an accurate pairing of each seedling with its corresponding plug hole, even in cases where adjacent seedling leaves encroached into neighboring plug holes. [Results and Discussions] The pruning process inevitably resulted in the loss of some parameters that were originally beneficial for feature representation and model generalization. This led to a noticeable decline in model performance. However, through meticulous fine-tuning, the model's feature expression capabilities were restored, compensating for the information loss caused by pruning. Experimental results demonstrated that the fine-tuned model not only maintained high detection accuracy but also achieved significant reductions in FLOPs (86.3%) and parameter count (95.4%). The final model size was only 1.2 MB. Compared to the original YOLOv8s model, the pruned version showed improvements in several key performance metrics: precision increased by 0.4%, recall by 1.2%, mAP by 1%, and the F₁-Score by 0.1%. The impact of the pruning rate on model performance was found to be non-linear. As the pruning rate increased, model performance dropped significantly after certain crucial channels were removed. However, further pruning led to a reallocation of the remaining channels' weights, which in some cases allowed the model to recover or even exceed its previous performance levels. Consequently, it was necessary to experiment extensively to identify the optimal pruning rate that balanced model accuracy and speed. The experiments indicated that when the pruning rate reached 85.7%, the mAP peaked at 96.4%. Beyond this point, performance began to decline, suggesting that this was the optimal pruning rate for achieving a balance between model efficiency and performance, resulting in a model size of 1.2 MB. To further validate the improved model's effectiveness, comparisons were conducted with different lightweight backbone networks, including MobileNetv3, ShuffleNetv2, EfficientViT, and FasterNet, while retaining the Neck and Head modules of the original YOLOv8s model. Results indicated that the modified model outperformed these alternatives, with mAP improvements of 1.3%, 1.8%, 1.5%, and 1.1%, respectively, and F₁-Score increases of 1.5%, 1.8%, 1.1%, and 1%. Moreover, the pruned model showed substantial advantages in terms of floating-point operations, model size, and parameter count compared to these other lightweight networks. To verify the effectiveness of the proposed two-stage seedling-hole matching algorithm, tests were conducted using a variety of complex images from the test set. Results indicated that the proposed method achieved precise grading and localization of strawberry seedlings even under challenging overgrowth conditions. Specifically, the correct matching rate for normal seedlings reached 96.6%, for missing seedlings 84.5%, and for weak seedlings 82.9%, with an average matching accuracy of 88%, meeting the practical requirements of the strawberry plug tray cultivation process. [Conclusions] The pruned YOLOv8s model successfully maintained high detection accuracy while reducing computational costs and improving inference speed. The proposed two-stage seedling-hole matching algorithm effectively minimized the interference caused by overgrown seedlings, accurately locating and classifying seedlings of various growth stages within the plug tray. The research provides a robust and reliable technical solution for automated strawberry seedling sorting in practical plug tray cultivation scenarios, offering valuable insights and technical support for optimizing the efficiency and precision of automated seedling grading systems.

[Objective] Spraying calcium can effectively prevent the occurrence of dry burning heart disease in Chinese cabbage. Accurately targeting spraying calcium can more effectively improve the utilization rate of calcium. Since the sprayer needs to move rapidly in the field, this can lead to over-application or under-application of the pesticide. This study aims to develop a targeted spray control system based on deep learning technology, explore the relationship between the advance speed, spray volume, and coverage of the sprayer, thereby addressing the uneven application issues caused by different nebulizer speeds by studying the real scenario of calcium administration to Chinese cabbage hearts. [Methods] The targeted spraying control system incorporates advanced sensors and computing equipment that were capable of obtaining real-time data regarding the location of crops and the surrounding environmental conditions. This data allowed for dynamic adjustments to be made to the spraying system, ensuring that pesticides were delivered with high precision. To further enhance the system's real-time performance and accuracy, the YOLOv8 object detection model was improved. A Ghost-Backbone lightweight network structure was introduced, integrating remote sensing technologies along with the sprayer's forward speed and the frequency of spray responses. This innovative combination resulted in the creation of a YOLOv8-Ghost-Backbone lightweight model specifically tailored for agricultural applications. The model operated on the Jetson Xavier NX controller, which was a high-performance, low-power computing platform designed for edge computing. The system was allowed to process complex tasks in real time directly in the field. The targeted spraying system was composed of two essential components: A pressure regulation unit and a targeted control unit. The pressure regulation unit was responsible for adjusting the pressure within the spraying system to ensure that the output remains stable under various operational conditions. Meanwhile, the targeted control unit played a crucial role in precisely controlling the direction, volume, and coverage of the spray to ensure that the pesticide was applied effectively to the intended areas of the plants. To rigorously evaluate the performance of the system, a series of intermittent spray tests were conducted. During these tests, the forward speed of the sprayer was gradually increased, allowing to assess how well the system responded to changes in speed. Throughout the testing phase, the response frequency of the electromagnetic valve was measured to calculate the corresponding spray volume for each nozzle. [Results and Conclusions] The experimental results indicated that the overall performance of the targeted spraying system was outstanding, particularly under conditions of high-speed operation. By meticulously recording the response times of the three primary components of the system, the valuable data were gathered. The average time required for image processing was determined to be 29.50 ms, while the transmission of decision signals took an average of 6.40 ms. The actual spraying process itself required 88.83 ms to complete. A thorough analysis of these times revealed that the total response time of the spraying system lagged by approximately 124.73 ms when compared to the electrical signal inputs. Despite the inherent delays, the system was able to maintain a high level of spraying accuracy by compensating for the response lag of the electromagnetic valve. Specifically, when tested at a speed of 7.2 km/h, the difference between the actual spray volume delivered and the required spray volume, after accounting for compensation, was found to be a mere 0.01 L/min. This minimal difference indicates that the system met the standard operational requirements for effective pesticide application, thereby demonstrating its precision and reliability in practical settings. [Conclusions] In conclusion, this study developed and validated a deep learning-based targeted spraying control system that exhibited excellent performance regarding both spraying accuracy and response speed. The system serves as a significant technical reference for future endeavors in agricultural automation. Moreover, the research provides insights into how to maintain consistent spraying effectiveness and optimize pesticide utilization efficiency by dynamically adjusting the spraying system as the operating speed varies. The findings of this research will offer valuable experiences and guidance for the implementation of agricultural robots in the precise application of pesticides, with a particular emphasis on parameter selection and system optimization.

[Significance] The explosive development of agricultural big data has accelerated agricultural production into a new era of digitalization and intelligentialize. Agricultural big data is the core element to promote agricultural modernization and the foundation of intelligent agriculture. As a new productive forces, big data enhances the comprehensive intelligent management decision-making during the whole process of grain production. But it faces the problems such as the indistinct management mechanism of grain production big data resources, the lack of the full-chain decision-making algorithm system and big data platform for the whole process and full elements of grain production. [Progress] Grain production big data platform is a comprehensive service platform that uses modern information technologies such as big data, Internet of Things (IoT), remote sensing and cloud computing to provide intelligent decision-making support for the whole process of grain production based on intelligent algorithms for data collection, processing, analysis and monitoring related to grain production. In this paper, the progress and challenges in grain production big data, monitoring and decision-making algorithms are reviewed, as well as big data platforms in China and worldwide. With the development of the IoT and high-resolution multi-modal remote sensing technology, the massive agricultural big data generated by the "Space-Air-Ground" Integrated Agricultural Monitoring System, has laid an important foundation for smart agriculture and promoted the shift of smart agriculture from model-driven to data-driven. However, there are still some issues in field management decision-making, such as the requirements for high spatio-temporal resolution and timeliness of the information are difficult to meet, and the algorithm migration and localization methods based on big data need to be studied. In addition, the agricultural machinery operation and spatio-temporal scheduling algorithm based on remote sensing and IoT monitoring information to determine the appropriate operation time window and operation prescription, needs to be further developed, especially the cross-regional scheduling algorithm of agricultural machinery for summer harvest in China. Aiming to address the issues of non-bi-connected monitoring and decision-making algorithms in grain production, as well as the insufficient integration of agricultural machinery and information perception, a framework for the grain production big data intelligent platform based on digital twins is proposed. The platform leverages multi-source heterogeneous grain production big data and integrates a full-chain suit of standardized algorithms, including data acquisition, information extraction, knowledge map construction, intelligent decision-making, full-chain collaboration of agricultural machinery operations. It covers the typical application scenarios such as irrigation, fertilization, pests and disease management, emergency response to drought and flood disaster, all enabled by digital twins technology. [Conclusions and Prospects] The suggestions and trends for development of grain production big data platform are summarized in three aspects: (1) Creating an open, symbiotic grain production big data platform, with core characteristics such as open interface for crop and environmental sensors, maturity grading and a cloud-native packaging mechanism for core algorithms, highly efficient response to data and decision services; (2) Focusing on the typical application scenarios of grain production, take the exploration of technology integration and bi-directional connectivity as the base, and the intelligent service as the soul of the development path for the big data platform research; (3) The data-algorithm-service self-organizing regulation mechanism, the integration of decision-making information with the intelligent equipment operation, and the standardized, compatible and open service capabilities, can form the new quality productivity to ensure food safety, and green efficiency grain production.

[Objective] The picking of famous and high-quality tea is a crucial link in the tea industry. Identifying and locating the tender buds of famous and high-quality tea for picking is an important component of the modern tea picking robot. Traditional neural network methods suffer from issues such as large model size, long training times, and difficulties in dealing with complex scenes. In this study, based on the actual scenario of the Xiqing Tea Garden in Hunan Province, proposes a novel deep learning algorithm was proposed to solve the precise segmentation challenge of famous and high-quality tea picking points. [Methods] The primary technical innovation resided in the amalgamation of a lightweight network architecture, MobilenetV2, with an attention mechanism known as efficient channel attention network (ECANet), alongside optimization modules including atrous spatial pyramid pooling (ASPP). Initially, MobilenetV2 was employed as the feature extractor, substituting traditional convolution operations with depth wise separable convolutions. This led to a notable reduction in the model's parameter count and expedited the model training process. Subsequently, the innovative fusion of ECANet and ASPP modules constituted the ECA_ASPP module, with the intention of bolstering the model's capacity for fusing multi-scale features, especially pertinent to the intricate recognition of tea shoots. This fusion strategy facilitated the model's capability to capture more nuanced features of delicate shoots, thereby augmenting segmentation accuracy. The specific implementation steps entailed the feeding of image inputs through the improved network, whereupon MobilenetV2 was utilized to extract both shallow and deep features. Deep features were then fused via the ECA_ASPP module for the purpose of multi-scale feature integration, reinforcing the model's resilience to intricate backgrounds and variations in tea shoot morphology. Conversely, shallow features proceeded directly to the decoding stage, undergoing channel reduction processing before being integrated with upsampled deep features. This divide-and-conquer strategy effectively harnessed the benefits of features at differing levels of abstraction and, furthermore, heightened the model's recognition performance through meticulous feature fusion. Ultimately, through a sequence of convolutional operations and upsampling procedures, a prediction map congruent in resolution with the original image was generated, enabling the precise demarcation of tea shoot harvesting points. [Results and Discussions] The experimental outcomes indicated that the enhanced DeepLabV3+ model had achieved an average Intersection over Union (IoU) of 93.71% and an average pixel accuracy of 97.25% on the dataset of tea shoots. Compared to the original model based on Xception, there was a substantial decrease in the parameter count from 54.714 million to a mere 5.818 million, effectively accomplishing a significant lightweight redesign of the model. Further comparisons with other prevalent semantic segmentation networks revealed that the improved model exhibited remarkable advantages concerning pivotal metrics such as the number of parameters, training duration, and average IoU, highlighting its efficacy and precision in the domain of tea shoot recognition. This considerable decreased in parameter numbers not only facilitated a more resource-economical deployment but also led to abbreviated training periods, rendering the model highly suitable for real-time implementations amidst tea garden ecosystems. The elevated mean IoU and pixel accuracy attested to the model's capacity for precise demarcation and identification of tea shoots, even amidst intricate and varied datasets, demonstrating resilience and adaptability in pragmatic contexts. [Conclusions] This study effectively implements an efficient and accurate tea shoot recognition method through targeted model improvements and optimizations, furnishing crucial technical support for the practical application of intelligent tea picking robots. The introduction of lightweight DeepLabV3+ not only substantially enhances recognition speed and segmentation accuracy, but also mitigates hardware requirements, thereby promoting the practical application of intelligent picking technology in the tea industry.

[Objective] Apple Alternaria leaf spot can easily lead to premature defoliation of apple tree leaves, thereby affecting the quality and yield of apples. Consequently, accurately detecting of the disease has become a critical issue in the precise prevention and control of apple tree diseases. Due to factors such as backlighting, traditional image segmentation-based methods for detecting disease spots struggle to accurately identify the boundaries of diseased areas against complex backgrounds. There is an urgent need to develop new methods for detecting apple Alternaria leaf spot, which can assist in the precise prevention and control of apple tree diseases. [Methods] A novel detection method named Deep Semi-Non-negative Matrix Factorization-based Mahalanobis Distance Anomaly Detection (DSNMFMAD) was proposed, which combines Deep Semi-Non-negative Matrix Factorization (DSNMF) with Mahalanobis distance for robust anomaly detection in complex image backgrounds. The proposed method began by utilizing DSNMF to extract low-rank background components and sparse anomaly features from the apple Alternaria leaf spot images. This enabled effective separation of the background and anomalies, mitigating interference from complex background noise while preserving the non-negativity constraints inherent in the data. Subsequently, Mahalanobis distance was employed, based on the Singular Value Decomposition (SVD) feature subspace, to construct a lesion detector. The detector identified lesions by calculating the anomaly degree of each pixel in the anomalous regions. The apple tree leaf disease dataset used was provided by PaddlePaddle AI-Studio. Each image in the dataset has a resolution of 512×512 pixels, in RGB color format, and was in JPEG format. The dataset was captured in both laboratory and natural environments. Under laboratory conditions, 190 images of apple leaves with spot-induced leaf drop were used, while 237 images were collected under natural conditions. Furthermore, the dataset was augmented with geometric transformations and random changes in brightness, contrast, and hue, resulting in 1 145 images under laboratory conditions and 1 419 images under natural conditions. These images reflect various real-world scenarios, capturing apple leaves at different stages of maturity, in diverse lighting conditions, angles, and noise environments. This diversed dataset ensured that the proposed method could be tested under a wide range of practical conditions, providing a comprehensive evaluation of its effectiveness in detecting apple Alternaria leaf spot. [Results and Discussions] DSNMFMAD demonstrated outstanding performance under both laboratory and natural conditions. A comparative analysis was conducted with several other detection methods, including GRX (Reed-Xiaoli detector), LRX (Local Reed-Xiaoli detector), CRD (Collaborative-Representation-Based Detector), LSMAD (LRaSMD-Based Mahalanobis Distance Detector), and the deep learning model Unet. The results demonstrated that DSNMFMAD exhibited superior performance in the laboratory environment. The results demonstrated that DSNMFMAD attained a recognition accuracy of 99.8% and a detection speed of 0.087 2 s/image. The accuracy of DSNMFMAD was found to exceed that of GRX, LRX, CRD, LSMAD, and Unet by 0.2%, 37.9%, 10.3%, 0.4%, and 24.5%, respectively. Additionally, the DSNMFMAD exhibited a substantially superior detection speed in comparison to LRX, CRD, LSMAD, and Unet, with an improvement of 8.864, 107.185, 0.309, and 1.565 s, respectively. In a natural environment, where a dataset of 1 419 images of apple Alternaria leaf spot was analysed, DSNMFMAD demonstrated an 87.8% recognition accuracy, with an average detection speed of 0.091 0 s per image. In this case, its accuracy outperformed that of GRX, LRX, CRD, LSMAD, and Unet by 2.5%, 32.7%, 5%, 14.8%, and 3.5%, respectively. Furthermore, the detection speed was faster than that of LRX, CRD, LSMAD, and Unet by 2.898, 132.017, 0.224, and 1.825 s, respectively. [Conclusions] The DSNMFMAD proposed in this study was capable of effectively extracting anomalous parts of an image through DSNMF and accurately detecting the location of apple Alternaria leaf spot using a constructed lesion detector. This method achieved higher detection accuracy compared to the benchmark methods, even under complex background conditions, demonstrating excellent performance in lesion detection. This advancement could provide a valuable technical reference for the detection and prevention of apple Alternaria leaf spot.

[Objective] Crop leaf area is an important indicator reflecting light absorption efficiency and growth conditions. This paper established a diverse Chinese yam image dataset and proposesd a deep learning-based method for Chinese yam leaf image segmentation. This method can be used for real-time measurement of Chinese yam leaf area, addressing the inefficiency of traditional measurement techniques. This will provide more reliable data support for genetic breeding, growth and development research of Chinese yam, and promote the development and progress of the Chinese yam industry. [Methods] A lightweight segmentation network based on improved ENet was proposed. Firstly, based on ENet, the third stage was pruned to reduce redundant calculations in the model. This improved the computational efficiency and running speed, and provided a good basis for real-time applications. Secondly, PConv was used instead of the conventional convolution in the downsampling bottleneck structure and conventional bottleneck structure, the improved bottleneck structure was named P-Bottleneck. PConv applied conventional convolution to only a portion of the input channels and left the rest of the channels unchanged, which reduced memory accesses and redundant computations for more efficient spatial feature extraction. PConv was used to reduce the amount of model computation while increase the number of floating-point operations per second on the hardware device, resulting in lower latency. Additionally, the transposed convolution in the upsampling module was improved to bilinear interpolation to enhance model accuracy and reduce the number of parameters. Bilinear interpolation could process images smoother, making the processed images more realistic and clear. Finally, coordinate attention (CA) module was added to the encoder to introduce the attention mechanism, and the model was named CBPA-ENet. The CA mechanism not only focused on the channel information, but also keenly captured the orientation and position-sensitive information. The position information was embedded into the channel attention to globally encode the spatial information, capturing the channel information along one spatial direction while retaining the position information along the other spatial direction. The network could effectively enhance the attention to important regions in the image, and thus improve the quality and interpretability of segmentation results. [Results and Discussions] Trimming the third part resulted in a 28% decrease in FLOPs, a 41% decrease in parameters, and a 9 f/s increase in FPS. Improving the upsampling method to bilinear interpolation not only reduces the floating-point operation and parameters, but also slightly improves the segmentation accuracy of the model, increasing FPS by 4 f/s. Using P-Bottleneck instead of downsampling bottleneck structure and conventional bottleneck structure can reduce mIoU by only 0.04%, reduce FLOPs by 22%, reduce parameters by 16%, and increase FPS by 8 f/s. Adding CA mechanism to the encoder could only increase a small amount of FLOPs and parameters, improving the accuracy of the segmentation network. To verify the effectiveness of the improved segmentation algorithm, classic semantic segmentation networks of UNet, DeepLabV3+, PSPNet, and real-time semantic segmentation network LinkNet, DABNet were selected to train and validate. These six algorithms got quite high segmentation accuracy, among which UNet had the best mIoU and the mPA, but the model size was too large. The improved algorithm only accounts for 1% of the FLOPs and 0.41% of the parameters of UNet, and the mIoU and mPA were basically the same. Other classic semantic segmentation algorithms, such as DeepLabV3+, had similar accuracy to improved algorithms, but their large model size and slow inference speed were not conducive to embedded development. Although the real-time semantic segmentation algorithm LinkNet had a slightly higher mIoU, its FLOPs and parameters count were still far greater than the improved algorithm. Although the PSPNet model was relatively small, it was also much higher than the improved algorithm, and the mIoU and mPA were lower than the algorithm. The experimental results showed that the improved model achieved a mIoU of 98.61%. Compared with the original model, the number of parameters and FLOPs significantly decreased. Among them, the number of model parameters decreased by 51%, the FLOPs decreased by 49%, and the network operation speed increased by 38%. [Conclusions] The improved algorithm can accurately and quickly segment Chinese yam leaves, providing not only a more accurate means for determining Chinese yam phenotype data, but also a new method and approach for embedded research of Chinese yam. Using the model, the morphological feature data of Chinese yam leaves can be obtained more efficiently, providing a reliable foundation for further research and analysis.

[Objective] Chinese kiwifruit texts exhibit unique dual-dimensional characteristics. The cross-paragraph dependency is complex semantic structure, whitch makes it challenging to capture the full contextual relationships of entities within a single paragraph, necessitating models capable of robust cross-paragraph semantic extraction to comprehend entity linkages at a global level. However, most existing models rely heavily on local contextual information and struggle to process long-distance dependencies, thereby reducing recognition accuracy. Furthermore, Chinese kiwifruit texts often contain highly nested entities. This nesting and combination increase the complexity of grammatical and semantic relationships, making entity recognition more difficult. To address these challenges, a novel named entity recognition (NER) method, KIWI-Coord-Prune(kiwifruit-CoordKIWINER-PruneBi-LSTM) was proposed in this research, which incorporated dual-dimensional information processing and pruning techniques to improve recognition accuracy. [Methods] The proposed KIWI-Coord-Prune model consisted of a character embedding layer, a CoordKIWINER layer, a PruneBi-LSTM layer, a self-attention mechanism, and a CRF decoding layer, enabling effective entity recognition after processing input character vectors. The CoordKIWINER and PruneBi-LSTM modules were specifically designed to handle the dual-dimensional features in Chinese kiwifruit texts. The CoordKIWINER module applied adaptive average pooling in two directions on the input feature maps and utilized convolution operations to separate the extracted features into vertical and horizontal branches. The horizontal and vertical features were then independently extracted using the Criss-Cross Attention (CCNet) mechanism and Coordinate Attention (CoordAtt) mechanism, respectively. This module significantly enhanced the model's ability to capture cross-paragraph relationships and nested entity structures, thereby generating enriched character vectors containing more contextual information, which improved the overall representation capability and robustness of the model. The PruneBi-LSTM module was built upon the enhanced dual-dimensional vector representations and introduced a pruning strategy into Bi-LSTM to effectively reduce redundant parameters associated with background descriptions and irrelevant terms. This pruning mechanism not only enhanced computational efficiency while maintaining the dynamic sequence modeling capability of Bi-LSTM but also improved inference speed. Additionally, a dynamic feature extraction strategy was employed to reduce the computational complexity of vector sequences and further strengthen the learning capacity for key features, leading to improved recognition of complex entities in kiwifruit texts. Furthermore, the pruned weight matrices become sparser, significantly reducing memory consumption. This made the model more efficient in handling large-scale agricultural text-processing tasks, minimizing redundant information while achieving higher inference and training efficiency with fewer computational resources. [Results and Discussions] Experiments were conducted on the self-built KIWIPRO dataset and four public datasets: People's Daily, ClueNER, Boson, and ResumeNER. The proposed model was compared with five advanced NER models: LSTM, Bi-LSTM, LR-CNN, Softlexicon-LSTM, and KIWINER. The experimental results showed that KIWI-Coord-Prune achieved F₁-Scores of 89.55%, 91.02%, 83.50%, 83.49%, and 95.81%, respectively, outperforming all baseline models. Furthermore, controlled variable experiments were conducted to compare and ablate the CoordKIWINER and PruneBi-LSTM modules across the five datasets, confirming their effectiveness and necessity. Additionally, the impact of different design choices was explored for the CoordKIWINER module, including direct fusion, optimized attention mechanism fusion, and network structure adjustment residual optimization. The experimental results demonstrated that the optimized attention mechanism fusion method yielded the best performance, which was ultimately adopted in the final model. These findings highlight the significance of properly designing attention mechanisms to extract dual-dimensional features for NER tasks. Compared to existing methods, the KIWI-Coord-Prune model effectively addressed the issue of underutilized dual-dimensional information in Chinese kiwifruit texts. It significantly improved entity recognition performance for both overall text structures and individual entity categories. Furthermore, the model exhibited a degree of generalization capability, making it applicable to downstream tasks such as knowledge graph construction and question-answering systems. [Conclusions] This study presents an novel NER approach for Chinese kiwifruit texts, which integrating dual-dimensional information extraction and pruning techniques to overcome challenges related to cross-paragraph dependencies and nested entity structures. The findings offer valuable insights for researchers working on domain-specific NER and contribute to the advancement of agriculture-focused natural language processing applications. However, two key limitations remain: 1) The balance between domain-specific optimization and cross-domain generalization requires further investigation, as the model's adaptability to non-agricultural texts has yet to be empirically validated; 2) the multilingual applicability of the model is currently limited, necessitating further expansion to accommodate multilingual scenarios. Future research should focus on two key directions: 1) Enhancing domain robustness and cross-lingual adaptability by incorporating diverse textual datasets and leveraging pre-trained multilingual models to improve generalization, and 2) Validating the model's performance in multilingual environments through transfer learning while refining linguistic adaptation strategies to further optimize recognition accuracy.

[Objective] Winter wheat yield is crucial for national food security and the standard of living of the population. Existing crop yield prediction models often show low accuracy under disaster-prone climatic conditions. This study proposed an improved hierarchical linear model (IHLM) based on a drought weather index reduction rate, aiming to enhance the accuracy of crop yield estimation under drought conditions. [Methods] HLM was constructed using the maximum enhanced vegetation index-2 (EVI2max), meteorological data (precipitation, radiation, and temperature from March to May), and observed winter wheat yield data from 160 agricultural survey stations in Shandong province (2018－2021). To validate the model's accuracy, 70% of the data from Shandong province was randomly selected for model construction, and the remaining data was used to validate the accuracy of the yield model. HLM considered the variation in meteorological factors as a key obstacle affecting crop growth and improved the model by calculating the relative meteorological factors. The calculation of relative meteorological factors helped reduce the impact of inter-annual differences in meteorological data. The accuracy of the HLM model was compared with that of the random forest (RF), Support Vector Regression (SVR), and Extreme Gradient Boosting (XGBoost) models. The HLM model provided more intuitive interpretation, especially suitable for processing hierarchical data, which helped capture the variability of winter wheat yield data under drought conditions. Therefore, a drought weather index reduction rate model from the agricultural insurance industry was introduced to further optimize the HLM model, resulting in the construction of the IHLM model. The IHLM model was designed to improve crop yield prediction accuracy under drought conditions. Since the precipitation differences between Henan and Shandong provinces were small, to test the transferability of the IHLM model, Henan province sample data was processed in the same way as in Shandong, and the IHLM model was applied to Henan province to evaluate its performance under different geographical conditions. [Results and Discussions] The accuracy of the HLM model, improved based on relative meteorological factors (rMF), was higher than that of RF, SVR, and XGBoost. The validation accuracy showed a Pearson correlation coefficient (r) of 0.76, a root mean squared error (RMSE) of 0.60 t/hm², and a normalized RMSE (nRMSE) of 11.21%. In the drought conditions dataset, the model was further improved by incorporating the relationship between the winter wheat drought weather index and the reduction rate of winter wheat yield. After the improvement, the RMSE decreased by 0.48 t/hm², and the nRMSE decreased by 28.64 percentage points, significantly enhancing the accuracy of the IHLM model under drought conditions. The IHLM model also demonstrated good applicability when transferred to Henan province. [Conclusions] The IHLM model developed in this study improved the accuracy and stability of crop yield predictions, especially under drought conditions. Compared to RF, SVR, and XGBoost models, the IHLM model was more suitable for predicting winter wheat yield. This research can be widely applied in the agricultural insurance field, playing a significant role in the design of agricultural insurance products, rate setting, and risk management. It enables more accurate predictions of winter wheat yield under drought conditions, with results that are closer to actual outcomes.

[Objective] The number, location, and crown spread of nursery stock are important foundations data for their scientific management. Traditional approach of conducting nursery stock inventories through on-site individual plant surveys is labor-intensive and time-consuming. Low-cost and convenient unmanned aerial vehicles (UAVs) for on-site collection of nursery stock data are beginning to be utilized, and the statistical analysis of nursery stock information through technical means such as image processing achieved. During the data collection process, as the flight altitude of the UAV increases, the number of trees in a single image also increases. Although the anchor box can cover more information about the trees, the cost of annotation is enormous in the case of a large number of densely populated tree images. To tackle the challenges of tree adhesion and scale variance in images captured by UAVs over nursery stock, and to reduce the annotation costs, using point-labeled data as supervisory signals, an improved dense detection and counting model was proposed to accurately obtain the location, size, and quantity of the targets. [Method] To enhance the diversity of nursery stock samples, the spruce dataset, the Yosemite, and the KCL-London publicly available tree datasets were selected to construct a dense nursery stock dataset. A total of 1 520 nursery stock images were acquired and divided into training and testing sets at a ratio of 7:3. To enhance the model's adaptability to tree data of different scales and variations in lighting, data augmentation methods such as adjusting the contrast and resizing the images were applied to the images in the training set. After enhancement, the training set consists of 3 192 images, and the testing set contains 456 images. Considering the large number of trees contained in each image, to reduce the cost of annotation, the method of selecting the center point of the trees was used for labeling. The LSC-CNN model was selected as the base model. This model can detect the quantity, location, and size of trees through point-supervised training, thereby obtaining more information about the trees. The LSC-CNN model was made improved to address issues of missed detections and false positives that occurred during the testing process. Firstly, to address the issue of missed detections caused by severe adhesion of densely packed trees, the last convolutional layer of the feature extraction network was replaced with dilated convolution. This change enlarges the receptive field of the convolutional kernel on the input while preserving the detailed features of the trees. So the model is better able to capture a broader range of contextual information, thereby enhancing the model's understanding of the overall scene. Secondly, the convolutional block attention module (CBAM) attention mechanism was introduced at the beginning of each scale branch. This allowed the model to focus on the key features of trees at different scales and spatial locations, thereby improving the model's sensitivity to multi-scale information. Finally, the model was trained using label smooth cross-entropy loss function and grid winner-takes-all strategy, emphasizing regions with highest losses to boost tree feature recognition. [Results and Discussions] The mean counting accuracy (MCA), mean absolute error (MAE), and root mean square error (RMSE) were adopted as evaluation metrics. Ablation studies and comparative experiments were designed to demonstrate the performance of the improved LSC-CNN model. The ablation experiment proved that the improved LSC-CNN model could effectively resolve the issues of missed detections and false positives in the LSC-CNN model, which were caused by the density and large-scale variations present in the nursery stock dataset. IntegrateNet, PSGCNet, CANet, CSRNet, CLTR and LSC-CNN models were chosen as comparative models. The improved LSC-CNN model achieved MCA, MAE, and RMSE of 91.23%, 14.24, and 22.22, respectively, got an increase in MCA by 6.67%, 2.33%, 6.81%, 5.31%, 2.09% and 2.34%, respectively; a reduction in MAE by 21.19, 11.54, 18.92, 13.28, 11.30 and 10.26, respectively; and a decrease in RMSE by 28.22, 28.63, 26.63, 14.18, 24.38 and 12.15, respectively, compared to the IntegrateNet, PSGCNet, CANet, CSRNet, CLTR and LSC-CNN models. These results indicate that the improved LSC-CNN model achieves high counting accuracy and exhibits strong generalization ability. [Conclusions] The improved LSC-CNN model integrated the advantages of point supervision learning from density estimation methods and the generation of target bounding boxes from detection methods.These improvements demonstrate the enhanced performance of the improved LSC-CNN model in terms of accuracy, precision, and reliability in detecting and counting trees. This study could hold practical reference value for the statistical work of other types of nursery stock.

[Objective] Plant leaf shape is an important part of plant architectural model. Establishment of a three-dimensional structural model of leaves may assist simulating and analyzing plant growth. However, existing leaf modeling approaches lack interpretability, invertibility, and operability, which limit the estimation of model parameters, the simulation of leaf shape, the analysis and interpretation of leaf physiology and growth state, and model reusage. Aiming at the interoperability between three-dimensional structure representation and mathematical model parameters, this study paid attention to three aspects in wheat leaf shape parametric reconstruction: (1) parameter-driven model structure, (2) model parameter inversion, and (3) parameter dynamic mapping during growth. Based on this, a set of parameter-driven and point cloud inversion model for wheat leaf interoperability was proposed in this study. [Methods] A parametric surface model of a wheat leaf with seven characteristic parameters by using parametric modeling technology was built, and the forward parametric construction of the wheat leaf structure was realized. Three parameters, maximum leaf width, leaf length, and leaf shape factor, were used to describe the basic shape of the blade on the leaf plane. On this basis, two parameters, namely the angle between stems and leaves and the curvature degree, were introduced to describe the bending characteristics of the main vein of the blade in the three-dimensional space. Two parameters, namely the twist angle around the axis and the twist deviation angle around the axis, were introduced to represent the twisted structure of the leaf blade along the vein. The reverse parameter estimation module was built according to the surface model. The point cloud was divided by the uniform segmentation method along the Y-axis, and the veins were fit by a least squares regression method. Then, the point cloud was re-segmented according to the fit vein curve. Subsequently, the rotation angle was precisely determined through the segment-wise transform estimation method, with all parameters being optimally fit using the RANSAC regression algorithm. To validate the reliability of the proposed methodology, a set of sample parameters was randomly generated, from which corresponding sample point clouds were synthesized. These sample point clouds were then subjected to estimation using the described method. Then error analyzing was carried out on the estimation results. Three-dimensional imaging technology was used to collect the point clouds of Zhengmai 136, Yangmai 34, and Yanmai 1 samples. After noise reduction and coordinate registration, the model parameters were inverted and estimated, and the reconstructed point clouds were produced using the parametric model. The reconstruction error was validated by calculating the dissimilarity, represented by the Chamfer Distance, between the reconstructed point cloud and the measured point cloud. [Results and Discussions] The model could effectively reconstruct wheat leaves, and the average deviation of point cloud based parametric reconstruction results was about 1.2 mm, which had a high precision. Parametric modeling technology based on prior knowledge and point cloud fitting technology based on posterior data was integrated in this study to construct a digital twin model of specific species at the 3D structural level. Although some of the detailed characteristics of the leaves were moderately simplified, the geometric shape of the leaves could be highly restored with only a few parameters. This method was not only simple, direct and efficient, but also had more explicit geometric meaning of the obtained parameters, and was both editable and interpretable. In addition, the practice of using only tools such as rulers to measure individual characteristic parameters of plant organs in traditional research was abandoned in this study. High-precision point cloud acquisition technology was adopted to obtain three-dimensional data of wheat leaves, and pre-processing work such as point cloud registration, segmentation, and annotation was completed, laying a data foundation for subsequent research. [Conclusions] There is interoperability between the reconstructed model and the point cloud, and the parameters of the model can be flexibly adjusted to generate leaf clusters with similar shapes. The inversion parameters have high interpretability and can be used for consistent and continuous estimation of point cloud time series. This research is of great value to the simulation analysis and digital twinning of wheat leaves.