Scientia Agricultura Sinica

Figure/Table detail

Identification of Hypovirus in Apple Ring Rot Fungus Botryosphaeria dothidea and Detection of Virus-Carrying Status in China

CONGQiQi, ZHANGJingYi, MENGXiangLong, DAIPengBo, LIBo, HUTongLe, WANGShuTong, CAOKeQiang, WANGYaNan

Scientia Agricultura Sinica, 2025, 58(3): 478-492. DOI: 10.3864/j.issn.0578-1752.2025.03.006

序号 Number	采集地点 Collection site	BdCV1		BdVV2
序号 Number	采集地点 Collection site	阳性菌株数/总菌株数 Positive number/total number	检出率 Detection rate (%)	阳性菌株数/总菌株数 Positive number/total number	检出率 Detection rate (%)
1-10	辽宁地区Liaoning region	6/10	60	5/10	50
11-23	山东地区Shandong region	9/13	69	4/13	30
24-30	河南三门峡Sanmenxia, Henan	3/7	43	1/7	14
31-50	河北地区Hebei region	8/20	40	3/20	15
51-55	陕西地区Shaanxi region	3/5	60	3/5	60
56	新疆阿克苏Akesu, Xinjiang	1/1	100	0/1	0
总计Total		30/56	53.6	16/56	28.6

Table 5 Distribution of BdCV1 and BdVV2 in B. dothidea from apple producing areas in China

Other figure/table from this article

Fig. 3 Positive monoclonal identification for strain WH-2L dsRNA cDNA library by PCR
Table 3 Information of virus type in strain WH-2L by cDNA library cloning
Table 4 Analysis of virus type in strain WH-2L by high-throughput sequencing
Table 1 Information of B. dothidea samples in this study
Fig. 1 dsRNA types in WH-2L and WH-3L strains of B. dothidea</i
Fig. 2 Electrophoresis of B. dothidea</i WH-2L dsRNA cDNA libraryM: DL2000; 1, 2: WH-2L; 3, 4: WH-3L
Fig. 4 Identification of eight viruses in strain WH-2L by RT-PCR
Fig. 8 Pathogenicity of B. dothideain vitro</i
Fig. 10 Detection of BdCV1 in the progeny of B. dothidea</i strain WH-2L by RT-PCR
Fig. 6 Pathogenicity of B. dothideain vitro</i
Fig. 12 Detection of horizontal transmission rate of BdCV1 among B. dothidea </istrains by RT-PCR
Fig. 13 Detection of horizontal transmission rate of BdVV2 among B. dothidea </istrains by RT-PCR
Fig. 7 Spot size on apple fruits infected by B. dothidea</i strains carrying different viruses of BdCV1 or BdVV2
Fig. 9 Spot size on pear fruits infected by B. dothidea</i strains carrying different viruses of BdCV1 or BdVV2
Fig. 5 RT-PCR detection of BdCV1 and BdVV2 in the representative strains of B. dothidea</i
Fig. 11 Detection of BdVV2 in the progeny of B. dothidea</i strain WH-2L by RT-PCR
Table 2 Primers used in this study