Scientia Agricultura Sinica

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The Effect of Krüppel-Like Factor 3 (KLF3) Gene on Bovine Fat Deposition

GUO HongFang,NING Yue,CHENG Gong,ZAN LinSen

Scientia Agricultura Sinica, 2019, 52(7): 1272-1281. DOI: 10.3864/j.issn.0578-1752.2019.07.014

Table 2 Comparison of bovine KLF3 protein with other GenBank recorded animals

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Fig. 3 KLF3 siRNA screening
A. Protein expression when treat with Si2-KLF3; B. KLF3 expression when treat with different SiRNA
Fig. 4 KLF3 relative expression after interfering with bovine KLF3 gene during adipocyte differentiation
** represented P<0.01. The same as below
Fig. 8 The content of triglyceride in bovine adipocyte when silence KLF3 gene at induced differentiation 4 days
Table 1 Primer used in the study
Fig. 1 Phylogenetic tree of bovine KLF3
Fig. 2 KLF3 gene expression pattern in Qinchuan newborn cattle tissues (A) and bovine adipocyte(B)
Fig. 5 The relative expression of PPARγ and C/EBPα when silence bovine KLF3 gene during bovine adipocyte differentiation
Fig. 6 The relative expression of ACCα, FAS and FABP4 when silence bovine KLF3 gene during bovine adipocyte differentiation
Fig. 7 Oil O staining when silence KLF3 gene at bovine adipocyte induced differentiation 4 days