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Relationship Between the Reproductive Ability of Hu Sheep andthe Expression Level of Genes Correlated with Ovulation
Relationship Between the Reproductive Ability of Hu Sheep andthe Expression Level of Genes Correlated with Ovulation
【Objective】 The present study was performed to investigate the relationships between the mRNA level of COX2, HAS2, TSG6 and PTX3 in ovaries and the reproductive ability of Hu Sheep, so as to provide references for finding hyperprolificacy candidate genes and understanding the molecular genetic mechanism of high fecundity in Hu sheep. 【Method】Sixteen adult Hu ewes, classified as high-yielding (H) or low-yiebding group (L), were sacrificed for tissue sampling between 24-36h after estrus diagnosis. The tissue distributions of COX2, HAS2, TSG6 and PTX3 mRNA were detected by RT-PCR and the gene expression levels in the ovaries were investigated by real-time PCR. 【Result】COX2, HAS2, TSG6 and PTX3 all expressed in ovary of Hu sheep. The expression level of COX2 mRNA in the ovary did not differ between H and L group (P>0.05), but TSG6 and PTX3 mRNA abundance in the ovary was extremely higher in H group than that in L group (P<0.01). In addition, HAS2 mRNA abundance in the ovary was extremely higher in L group than that in H group (P<0.01). 【Conclusion】 The results suggested that HAS2, TSG6 and PTX3 might play a key role in reproductive ability of Hu Sheep and might be candidate genes for high fecundity in Hu sheep.
Hu sheep /
reproductive ability /
genes correlated with ovulation /
mRNA expression
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