Phenotypic and Genetic Diversity of Rhizobia Associated with Glycyrrhiza spp. Grown in Northern Regions of China

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Scientia Agricultura Sinica ›› 2006, Vol. 39 ›› Issue (7) : 1321-1327. DOI: 10.3864/j.issn.0578-1752.at-2005-5837
CROP GENETICS & BREEDING·GERMPLASM RESOURCES

Phenotypic and Genetic Diversity of Rhizobia Associated with Glycyrrhiza spp. Grown in Northern Regions of China

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Abstract

【Objective】 The biodiversity of rhizobia from Glycyrrhiza spp. in northern regions of China was studied. 【Method】Twenty isolates from Glycyrrhiza spp. were characterized by numerical taxonomy, 16S rDNA PCR-RFLP analysis and BOX-PCR fingerprinting to describe the phenotypic and genetic diversity.【Result】There were two clusters at 85% similarity by numerical taxonomy and 11 strains are separate from reference strains. Fourteen genotypes of tested strains were obtained from the tested 20 strains and analyzed by 16S rDNA PCR-RFLP. The diversity of genomic level was described by BOX-PCR fingerprinting.【Conclusion】 All analyzed results of analysis desmonstrated that the symbiotic bacteria associated with Glycyrrhiza spp. could be found in the genera of Sinorhizobium, Rhizobium and Mesorhizobium.

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rhizobia / numerical taxonomy / 16S rDNA PCR-RFLP / BOX-PCR fingerprinting

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,. Phenotypic and Genetic Diversity of Rhizobia Associated with Glycyrrhiza spp. Grown in Northern Regions of China. Scientia Agricultura Sinica. 2006, 39(7): 1321-1327 https://doi.org/10.3864/j.issn.0578-1752.at-2005-5837
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