PCR-RFLP was firstly applied to analyze the correlation of polymorphisms of POU1F1 gene and growth traits in 164 beef cattle, at the age of 4 months (±10 d), including Qinchuan cattle, Qinchuan×Limousin cattle, Qinchuan×Red Angus and Qinchuan×German Yellow cattle. The results showed that 451 bp PCR products digested with HinfI demonstrated polymorphisms in four populations which were at Hardy-Weinberg equilibrium (P>0.05). Moreover, the frequencies of allele A/B of QQ, AQ, DQ and LQ populations were 0.232/0.768, 0.333/0.667, 0.178/0.822 and 0.181/0.819, respectively. The correlation of polymorphisms of POU1F1 gene and growth traits among the 4 populations was analyzed. In heart girth, height at hip cross, individuals with genotype AB and BB in population were higher than that of genotype AA, that is, BB and AB＞AA (P＜0.05). So POU1F1 gene can be one of a candidate genes for growth traits with heart girth, height at hip cross, but not for body length trait. The conclusion is that genotype BB is a predominant genotype and B is a predominant allele.