【Objective】The hypersensitive response (HR) in rice suspension cultured cells induced by exogenous addition of the signal molecules, nitric oxide (NO) and hydrogen peroxide (H2O2) was studied. 【Method】The HR of rice cells induced by NO and H2O2 dependently and/or in synergy, was quantitatively determined by the bromphenol blue staining method.【Result】Results showed that HR of rice cells could be induced by NO and H2O2 dependently and/or in synergy and that NO-induced HR could be regulated by H2O2. The balance interaction in concentrations between NO and H2O2 had no influence on the induction of HR.【Conclusion】NO and H2O2 were the signal molecules for the induction of the rice cells HR and the balance model for NO and H2O2 interaction was not proved in the induction of the HR of rice cells.