Isolation and characterization of melanin from rapeseed meal
Xuli Ma, Xuan Li, Fan Chen, Wenchao Chen, Xia Wan, Fenghong Huang
Author information+
Oil Crops Research Institute, Chinese Academy of Agricultural Sciences;The Faculty of Life Sciences, Hubei University;Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture;Oil Crops and Lipids Process Technology National & Local Joint Engineering Laboratory;Hubei Key Laboratory of Lipid Chemistry and Nutrition
{{custom_zuoZheDiZhi}}
{{custom_authorNodes}}
{{custom_bio.content}}
{{custom_bio.content}}
Collapse
History+
Published
2019-03-30
Issue Date
2019-03-30
Abstract
Melanin involves in various biological functions, widely used in cosmetic, pharmacology medicine and other fields. However, melanin application is limited due to low productivity and high cost. In this work, melanin extraction method from rapeseed meal(RSM) was explored. Effects of hydrochloric acid(HCl) concentration, ethanol concentration, extraction temperature and time, and solid-liquid ratio were evaluated systematically. According to results of single factor experiment and Box-Behnken experiment, the optimum extraction conditions of melanin from RSM were as follows: HCl concentration, 0.5 mol/L; ethanol concentration, 70%; extraction temperature, 40 o C; extraction time, 1 h; solid liquid ratio, 1/4 g/mL. Under these conditions, extraction yields of crude melanin(CM) and purified melanin(PM) were 9.00% and 1.44%, respectively. Compared with synthetic melanin(SM) from Sigma, the relative purities of CM and PM were 7.82% and 29.57%, respectively. Moreover, feedstuff value of RSM residue was also improved after melanin isolation by significantly reducing glucosinolate content.