HOU Donghai, YIN Ming, CHEN Xiaolan, YU Hong
Using simultaneous distillation extraction (SDE) and gas chromatography-mass spectrometry (GC-MS),10 mycelium samples of Cordyceps militaris collected in seven places in China were measured for their volatile components. A total of 65 volatile components were identified,and they mainly included acids,esters,naphthalenes,alkanes,aldehydes and anthracenes with an average content of 71.16%,10.57%,5.25%,5.13%,4.04% and 1.23%,respectively. Eight components,linoleic acid,palmitic acid,naphthalene,hexanal,massoia lactone,α-pinene,methyl linoleate and 2-pentylfuran,were detected in all samples,and their average contents were 37.41%,29.09%,5.25%,3.26%,2.65%,2.27%,1.53% and 0.65%,respectively. Some typical medicinal volatile substances,such as caryophyllin,a-cadinol,a-cedrene,massoia lactone,were also detected. As shown by principal component analysis (PCA) and clustering analysis (CA),there were large differences in volatile components of samples from Yunnan and Liaoning. Relative odor activity value (ROAV) analysis showed that aldehydes contributed the most to the odor of C. militaris mycelium,and other key aroma components included hexanal and 2-pentylfuran. There were differences in mycelial odor characteristics of C. militaris from different production regions.