Effect of Culture Condition on Sporulation of Wolfiporia cocos

ZENG Guiping, LI Zhong, ZHANG Wenlong, ZHAO Zhi,

PDF(440 KB)
PDF(440 KB)
Acta Edulis Fungi ›› 2018, Vol. 25 ›› Issue (04) : 43-46. DOI: 10.16488/j.cnki.1005-9873.2018.04.007
Original Paper

Effect of Culture Condition on Sporulation of Wolfiporia cocos

  • ZENG Guiping;LI Zhong;ZHANG Wenlong;ZHAO Zhi
Author information +
History +

Abstract

In order to explore optimal medium and culture conditions for sporulation of Wolfiporia cocos,effect of medium component, temperature, initial pH after autoclave and light condition were studied. The results showed that the optimal conditions for sporulation were PDA medium(350 mL with 60 mL medium)composed of 200 g/L potato, 20 g/L glucose, 1 g/LKH2?PO4, 0.5 g/L MgSO4·7 H2?O, 10 mg/L VB1, and18 g/L agar with natural initial pH at 6.5, cultivation at 28 ℃ in the dark for 7 days followed with a light cycle of 12 h/12 h(irradiation/in the dark). Spore yield under the optimal condition was(8.12 ± 0.11) x 109?per flask on day 38.?

Key words

?Wolfiporia cocos / sexual spore / sporulation

Cite this article

Download Citations
ZENG Guiping, LI Zhong, ZHANG Wenlong, ZHAO Zhi,. Effect of Culture Condition on Sporulation of Wolfiporia cocos. Acta Edulis Fungi. 2018, 25(04): 43-46 https://doi.org/10.16488/j.cnki.1005-9873.2018.04.007
Share on Mendeley
PDF(440 KB)

Accesses

Citation

Detail

Sections
Recommended

/