Cloning and Sequencing of a Putative Mitochondrial Intermediate Peptidase Gene Fragment from the Edible Fungus Lentinula edodes

BAO Dapeng, CHEN Mingjie, ZHANG Meiyan(Shanghai Biotechnology and Engineering Research Center for the Edible Fungi;Key Laboratory of AgriculturalGenetics and Breeding of Shanghai; Institute of Edible Fungi, Shanghai Academy of AgriculturalSciences; Shangh

Acta Edulis Fungi ›› 2007, Vol. 14 ›› Issue (04) : 8-11. DOI: 10.16488/j.cnki.1005-9873.2007.04.002
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Cloning and Sequencing of a Putative Mitochondrial Intermediate Peptidase Gene Fragment from the Edible Fungus Lentinula edodes

  • BAO Dapeng, CHEN Mingjie, ZHANG Meiyan(Shanghai Biotechnology and Engineering Research Center for the Edible Fungi;Key Laboratory of AgriculturalGenetics and Breeding of Shanghai; Institute of Edible Fungi, Shanghai Academy of AgriculturalSciences; Shangh
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Abstract

A 531 bp fragment of a putative mitochondrial intermediate peptidase (mip) gene from Lentinula edodes was amplified by PCR using a pair of degenerate primers designed according to conserved sequences of mip genes reported in other Basidiomycetes. Alignment analysis revealed that this putative partial mip gene from L. edodes exhibited high homology with corresponding segments of mip genes from the other Basidiomycetes. Since the gene encoding MIP appears to be very closely linked with the A type mating gene of Basidiomycetes, identification of mip may prove a useful tool for locating and cloning the A type mating gene in L. edodes and other edible fungi.

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Lentinula edodes, Mating type gene, A factor, Mitochondrial intermediate peptidase (mip) gene

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BAO Dapeng, CHEN Mingjie, ZHANG Meiyan(Shanghai Biotechnology and Engineering Research Center for the Edible Fungi;Key Laboratory of AgriculturalGenetics and Breeding of Shanghai; Institute of Edible Fungi, Shanghai Academy of AgriculturalSciences; Shangh. Cloning and Sequencing of a Putative Mitochondrial Intermediate Peptidase Gene Fragment from the Edible Fungus Lentinula edodes. Acta Edulis Fungi. 2007, 14(04): 8-11 https://doi.org/10.16488/j.cnki.1005-9873.2007.04.002
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