Cloning of a DNA Fragment Correlated to the Quality of Agaricus bisporus Fruitbody

Song Siyang1Zeng Wei1,2Chen Rong1Wang Zesheng2Su Wenjin1(1 State Laboratory for Tumor Cell Engineering, Xiamen University, Xiamen361005;2Fujian Research Institute of Light Industry, Fuzhou350005)

Acta Edulis Fungi ›› 2000, Vol. 07 ›› Issue (03) : 12-16. DOI: 10.16488/j.cnki.1005-9873.2000.03.003
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Cloning of a DNA Fragment Correlated to the Quality of Agaricus bisporus Fruitbody

  • Song Siyang1Zeng Wei1,2Chen Rong1Wang Zesheng2Su Wenjin1(1 State Laboratory for Tumor Cell Engineering, Xiamen University, Xiamen361005;2Fujian Research Institute of Light Industry, Fuzhou350005)
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Abstract

With 20 ten-mers random primers, the genomic differences between two types of Agaricus bisporus strain-good quality with low yield (G type) vs. poor quality with high yield (H type), were displayed by Random Amplified Polymorphic DNA (RAPD) technique. A 2000bp specific DNA fragment correlated to the quality of Agaricus bisporusfruitbody was obtained and cloned. Then dot hybridization and RFLP technique were used to determine the effectiveness of distinguishing two types of A. bisporus strains. And it was considered that our study had laid a foundation for further establishing a specific probe or PCR primer and for improving the accuracy of early predicting the characteristics of A. bisporus strains at molecular level.

Key words

Agaricus bisporus, RAPD, Fruitbody, Cloning, RFLP

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Song Siyang1Zeng Wei1,2Chen Rong1Wang Zesheng2Su Wenjin1(1 State Laboratory for Tumor Cell Engineering, Xiamen University, Xiamen361005;2Fujian Research Institute of Light Industry, Fuzhou350005). Cloning of a DNA Fragment Correlated to the Quality of Agaricus bisporus Fruitbody. Acta Edulis Fungi. 2000, 07(03): 12-16 https://doi.org/10.16488/j.cnki.1005-9873.2000.03.003
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