In present study，ISSR-PCR reaction conditions on Gossypium spp. were optimized. The factors which affect ISSR results of Gossypium spp. were investigated by uniform designs of two times. The results showed that 0.5 U Taq polymerase，40 ng template DNA，0.25 mmol·L-1 dNTPs，0.5 μmol·L-1 primer and 2.0 mmol·L-1 Mg2+ in 20 μL ISSR reaction system might be the best combination. To validate the ISSR-PCR reaction system above，eight cultivars of Gossypium and three ISSR primers were employed in the research. It is indicated that the ISSR reaction system would be more productive and repeatable
Key words
cotton
/
ISSR /
system optimization /
uniform design
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