Studies of Salinity-tolerance with SSR Markers on G.hirsutum L.
ZHANG Li-na, YE Wu-wei*, WANG Jun-juan, FAN Bao-xiang
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Cotton Research Institute, Chinese Academy of Agricultural Sciences; Key Laboratory of Cotton Genetic Improvemet, Ministry of Agriculture, Anyang, Henan 455000, China
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Received
Published
2009-04-21
2010-03-15
Issue Date
2010-03-15
Abstract
Two typical salinity tolerance varieties(Zhong 07 and CCRI 35) and two typical salinity sensitive varieties(Xinyan 96-48 and CCRI 12), were tested by SSR method in order to analyze the salinity-related SSR markers. 274 pairs of SSR primers with polymorphism on the four cotton varieties were screened from 3528 pairs of SSR primers. Especially, 10 pairs of them such as Y01, Y02, Y03, Y04, Y05, Y06, Y07, Y08, Y09, Y10 could distinguish salinity-tolerance varieties from salinity-sensitive varieties, each of those showed in the electrophoregram showed that the same primer amplified different DNA bands between the two salinity-tolerance varieties and the other two salinity-sensitive varieties, thus can identify salinity tolerance between different cotton varieties from molecular background. These results are useful to develop molecular identification method of salinity-tolerance about cotton germplasm.
ZHANGLi-Na, YEWu-Wei, WANGJun-Juan, FANBao-Xiang.
Studies of Salinity-tolerance with SSR Markers on G.hirsutum L.. Cotton Science. 2010, 22(2): 175-180 https://doi.org/10.11963/cs100214
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