Expression of a GAI Gene of Cotton (Gossypium hirsutum) and Preparation of Its Antibody

ZHENGYin-Ying, CUIBai-Ming, ZHUJian-Bo, LIAOWen-Bin, ZHANGGen-Fa- , PENG Ming

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Cotton Science ›› 2009, Vol. 21 ›› Issue (6) : 520-523. DOI: 10.11963/cs090615
Article

Expression of a GAI Gene of Cotton (Gossypium hirsutum) and Preparation of Its Antibody

  • ZHENG Yin-ying1,2,3, CUI Bai-ming2,3, ZHU Jian-bo3, LIAO Wen-bin2, ZHANG Gen-fa4, PENG Ming1,2,3*
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Abstract

DELLA protein is the key negative regulator of GA signal response. In order to study the effect of GA on development of cotton fiber, we cloned GhGAI gene, a member of DELLA gene family from the upland cotton EST database by electronic cloning method based on EST. According to the electronic cloning sequence, an integrate coding region was amplified from cotton(Gossypium hirsutum) ovule cDNA. A protein band with molecular weight (Mr) of 62000 was obtained from the prokaryotic expression products by SDS-PAGE. The protein was then purified by His-tag affinity chromatography and used for mutiple-cloned antibody preparation by rabbit immunization. Indirect ELISA test and the Western blot analysis showed that the anti-serum was in high immunocompetence and high specificity.

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Gossypium hirsutum

/ GhGAI gene / polyclonal antibody / Western blot

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ZHENGYin-Ying, CUIBai-Ming, ZHUJian-Bo, LIAOWen-Bin, ZHANGGen-Fa- , PENG Ming. Expression of a GAI Gene of Cotton (Gossypium hirsutum) and Preparation of Its Antibody. Cotton Science. 2009, 21(6): 520-523 https://doi.org/10.11963/cs090615

References

［1］ SWAIN S M, Olszewski N E. Genetic analysis of gibberellin signal transduction［J］. Plant Physiol, 1996, 112(1):11-17. ［2］ PENG Jin-rong, Harberd N P. Gibberellin deficiency and response mutations suppress the stem elongation phenotype of phytochrome-deficient mutants of Arabidopsis［J］. Plant Physiol, 1997,113(4):1051-1058. ［3］ SILVERTONE A L, Ciampaglio C N, Sun Tai-ping. The Arabidopsis RGA gene encodes a transcriptional regulator repressing the gibberellin signal transduction pathway［J］. Plant Cell, 1998, 10(2):155-169. ［4］ SINGH D P, Jermakow A M, Swain S M. Gibberellins are required for seed development and pollen tube growth in Arabidopsis［J］. Plant Cell, 2002, 14(12):3133-3147. ［5］ CAO Dong-ni, Hussain A, Cheng Hui, et al. Loss of function of four DELLA genes leads to light and gibberellin independent seed germination in Arabidopsis［J］. Planta, 2002,5(5):376-381. ［6］ YU Hao, Ito T, Zhao Yuan-xiang, et al. Floral homeotic genes are targets of gibberellin signaling in flower development［J］. PNAS, 2004, 101(20):7827-7832. ［7］ TYLER L, Thomas S G, Hu Jian-hong, et al. Della proteins and gibberellin-regulated seed germination and floral development in Arabidopsis［J］. Plant Physiol, 2002, 16(5):646-658. ［8］ FU Xiang-dong, Harberd N P. Auxin promotes Arabidopsis root growth by modulating gibberellin response［J］. Nature, 2003, 421:740-743. ［9］ 丁锦平，刘冬梅，周瑞阳，等. 2种棉花叶片总蛋白质纯化方法比较研究［J］. 河南师范大学学报: 自然科学版, 2009,37(5):103-107.                 DING Jin-ping, Liu Dong-mei, Zhou Rui-yang, et al. Comparison of two purification methods of cotton leaf total protein［J］. Journal of Henan Normal University: Natural Science, 2009,37(5):103-107. ［10］ BEASLEY C A, Ting I P. Effects of plant growth substances on in vitro fiber development from unfertilized cotton ovules［J］. American Journal of Botany, 1974,61(2):188-194. ［11］ PENG Jin-rong, Richards D E, Hartley N, et al. 'Green revolution' genes encode mutant gibberellin response modulators［J］. Nature, 1999, 400(6741):256-261. ［12］ 于晓玲, 崔百明, 卫海滨, 等. 基因枪转化法在棉纤维细胞中瞬时表达外源基因的研究［J］. 棉花学报, 2007, 19(6):419-423.                YU Xiao-ling, Cui Bai-ming, Wei Hai-bin, et al. Study on transient expression of target gene in the epidermal  cells of cotton ovules via particle bombardment［J］. Cotton Science, 2007,19(6): 419-423.[
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