Morphological and anatomic characters of the flowers and anthers in a new kind of cytoplasmic male sterile (CMS) of three accessions, named G22A, H109A, and 1793A, were studied in this paper. As the results shown that all the three new CMS accessions were completely malesterile, their flowers were the characters of smaller petals, shorter filaments, and no pollens, without any difference among them. The anatomical and cytological observation showed that the abortion of auxocyte cells in the three CMS lines took place between the sporogneous cell division to the beginning of PMC meiosis. The most significant difference in anatomical and cytological characters between the new CMS lines and their fertile genetic background lines, named G22B, H109B, and 1973B, was the development of the tapetum cells. Comparing with the tapetal cells of the fertile lines, those of the sterile anthers were much smaller with lightly stained after dyeing. The size of those cells was almost as big as that of middle layer cells. There was no abortion process for the CMS tapetum cells during the anther development, while those of their fertile genetic background lines were darkly stained, bigger in size, and degenerated gradually during the anther development, which was important for the development of anther to produce fertile pollens. So the abnormal development of tapetum cells was probably the reason led to the sterility of pollens for the new CMS lines.
Key words
anatomy
/
cytoplasmic male sterility /
cytology /
Gossypium hirsutum
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