河南省不同地区棉花黄萎病菌分离物致病性及其毒素致萎活性测定

桑　茜， 袁虹霞， 王振跃， 李洪连， 汪　敏

棉花学报. 2010, 22(4): 333-338

PDF(382 KB)
PDF(382 KB)
棉花学报 ›› 2010, Vol. 22 ›› Issue (4) : 333-338. DOI: 10.11963/cs100407
研究与进展

河南省不同地区棉花黄萎病菌分离物致病性及其毒素致萎活性测定

  • 桑　茜，袁虹霞，王振跃，李洪连，汪　敏*
作者信息 +

Pathogenicity of Verticillium dahliae Cotton Isolates in Henan Province and the Wilting Capability of Their Crude Toxins

  • SANG Xi, YUAN Hong-xia, WANG Zhen-yue, LI Hong-lian, WANG Min*
Author information +
History +

摘要

在河南省不同地区分离并鉴定33株棉花黄萎病菌分离物，并进行了致病力和粗毒素致萎活性测定。致病力结果表明，河南省棉花黄萎病菌分离物可分为强、中、弱3种致病力类型，分别占48.5 %、21.2 %、30.3 %。河南省同一地区内不同分离物间致病力差异明显。粗毒素致萎活性测定结果表明，黄萎病菌强致病力分离物分泌的粗毒素对棉苗的致萎力较强，弱致病力分离物分泌的粗毒素对棉苗的致萎力较弱，强、中、弱3种致病力类型分离物在72 h时对棉苗的平均致萎指数分别为63.82、52.38、45.83。采用考马斯亮蓝法测定不同分离物粗蛋白含量，结果表明，不同黄萎病菌分离物分泌的粗蛋白含量存在明显差异，且与其所致的病情指数呈显著正相关。

Abstract

In order to research pathogenicity differentiation of Verticillium dahliae on cotton in Henan, 33 Verticillium dahliae isolates were isolated and identified from the main cotton-producing regions in Henan Province. The results of pathogenicity tests on cotton seedings indicated that the V. dahliae isolates from cotton in Henan could be divided into three pathotypes: the highly aggressive isolates, the moderate aggressive ones and the weakly aggressive ones, accounting for 48.5%, 21.2% and 30.3%, respectively. The results also showed that the pathogenicity of isolates had no significant difference among different regions, but the pathogenicity of isolates from the same region showed significant difference. The wilting capability of the crude toxin secreted by V. dahliae isolates were also tested, the results showed that the crude toxin of different isolates had different wilting capability; the crude toxin secreted by the highly aggressive isolates had strong wilting capability. The average wilt index of the highly aggressive isolates, moderate aggressive ones and weakly aggressive ones at 72 h were 63.82, 52.38 and 45.83 respectively at the same concentration. The crude protein contents secreted by different isolates were also tested by the Coomassie Brilliant Blue G250 method. The crude protein contents of different isolates of V. dahliae had a significant positive correlation with the pathogenicity index. Highly aggressive isolates secreted more proteins than the weakly aggressive ones.

关键词

棉花黄萎病

/ 大丽轮枝菌 / 致病力 / 毒素

Key words

cotton Verticillium wilt

/ Verticillium dahliae / pathogenicity / toxin

引用本文

EndNote

Ris (Procite)

Bibtex

导出引用
桑　茜， 袁虹霞， 王振跃， 李洪连， 汪　敏. 河南省不同地区棉花黄萎病菌分离物致病性及其毒素致萎活性测定. 棉花学报. 2010, 22(4): 333-338 https://doi.org/10.11963/cs100407
SANG　Qian, YUANHong-Xia, WANGZhen-Yue, LIHong-Lian, WANG　Min. Pathogenicity of Verticillium dahliae Cotton Isolates in Henan Province and the Wilting Capability of Their Crude Toxins. Cotton Science. 2010, 22(4): 333-338 https://doi.org/10.11963/cs100407

参考文献

[1] 张改英，徐恒玉．棉花黄萎病及其综合防治技术[J]．河南农业科学, 2002(9): 27-28.        ZHANG Gai-ying, Xu Heng-yu. Cotton Verticillium wilt and its integrated prevention and control technique[J]. Henan Agricultural Sciences, 2002(9): 27-28. [2] 景忆莲，刘耀斌，万法，等．棉花黄萎病及其抗性育种研究进展[J]．西北农业学报, 1999, 8(3): 106-110.      JING Yi-lian, Liu Yao-bin, Fan Wan-fa, et al. Advance in the study on Verticillium wilt of cotton and its breeding for resistance[J]. Acta Agriculturae Boreali-occidentalis Sinica, 1999, 8(3): 106-110. [3] 朱荷琴，宋晓轩，简桂良．棉花黄萎病菌致病力变异生理机制的初步研究[J]．棉花学报, 2004, 16(5): 275-279.     ZHU He-qin, Song Xiao-xuan, Jian Gui-liang. The preliminary study of variation mechanism of pathogenic type of Verticillium dahliae Kleb[J]. Cotton Science, 2004, 16(5): 275-279. [4] 刘学堂，宋晓轩，郭金城．棉花黄萎病菌的研究及最新进展[J]．棉花学报, 1998, 10(1): 6-13.      LIU Xue-tang, Song Xiao-xuan, Guo Jin-cheng. Studies and advances on cotton Verticillium wilt[J]. Cotton Science, 1998, 10(1): 6-13. [5] WANG Jian-ying, Yu Cai, Gou Jing-ying, et al. VdNEP, an elicitor from Verticillium dahliae, induces cotton plant wilting[J]. Applied and Environmental Microbiology, 2004, 70(8): 4989- 4995. [6] KEEN N T, Erwin D C. Endopolygalacturonase: evidence against involvement in Verticillium wilt of cotton[J]. Phytopathology, 1971, 61: 198-203. [7] 章元寿，王建新，刘经芬，等．大丽轮枝菌毒素的分离、提纯及生物测定[J]．真菌学报, 1989, 8(2): 140-147.      ZHANG Yuan-shou, Wang Jian-xin, Liu Jing-fen, et al. Studies on the isolation, purification and bioassay of toxin from Verticillium dahliae Kleb[J]. Acta Mycologica Sinica, 1989, 8(2): 140- 147. [8] 宋晓轩，朱荷琴，郭金城．棉花黄萎病菌(Verticillium dahliae K1eb. )安阳分离物致病力分化研究[J]．中国农业科学, 1997, 30(1): 13-18.    SONG Xiao-xuan, Zhu He-qin, Guo Jin-cheng. Study on pathogenicity differentiation for Verticillium dahliae Kleb. Anyang strains in cotton[J]. Scientia Agricultura Sinica, 1997, 30(1): 13-18. [9] 朱荷琴，宋晓轩，孙君灵，等． 棉花黄萎病菌安阳菌系致病类型变异研究[J]. 棉花学报, 1999, 11(6): 312-317.     ZHU He-qin, Song Xiao-xuan, Sun Jun-ling, et al. Study on pathogenic type in Anyang strains of Verticillium dahliae Kleb[J]. Cotton Science, 1999, 11(6): 312-317. [10] 杨凤祥，桑　茜，马奇祥，等．河南省棉花黄萎病菌培养特性与致病力分化研究[J]．河南农业科学, 2009 (6): 85-89.       YANG Feng-xiang, Sang Xi, Ma Qi-xiang, et al. Study on the cultural characteristics and pathogenic differentiation of Verticillium dahliae from cotton in Henan[J]. Henan Agricultural Sciences, 2009(6): 85-89. [11] 石磊岩，王　波，文　学． 我国棉花黄萎病菌类型分化及培养特性研究[J]．植物保护学报, 1993, 20(3): 247-252.        SHI Lei-yan, Wang Bo, Wen Xue. Study on type differentiation and cultural characteristics of cotton Verticillium wilt in China[J]. Acta Phytophylacica Sinica, 1993, 20(3): 247-252. [12] 甘　莉，吕金殿．棉花黄萎病菌毒素的分离提纯及致萎活性测定[J]．西北农业大学学报, 1991, 19(1): 49-56.        GAN Li, Lü Jin-dian. Studies on the isolation, purification and wilting activity determination of toxin from Verticillium dahliae Kleb[J]. Acta Universitatis Agriculturalis Boreali-occidentalis, 1993, 20(3): 247-252. [13] 张慧霞，许　楠，杨家荣，等．棉花黄萎病菌毒素的提取及其生物活性测定[J]．西北农业学报, 2008, 17 (3): 312-314.       ZHANG Hui-xia, Xu Nan, Yang Jia-rong, et al. Extraction and bioassay of the toxin produced by Verticillium dahliae[J]. Acta Agriculturae Boreali-occidentalis Sinica, 2008, 17(3): 312-314. [14] BRADFORD M M. A rapid and sensitive method for the quantization of microgram quantities of protein utilizing the principle of protein-dye binding[J]. Analytical biochemistry, 1976, 72: 248-254. [15] LI Ying-zhang, Zheng Xiao-hua, Tang Hai-lin, et al. Increase of β-1,3-glucanase and chitinase activities in cotton callus cells treated by salicylic acid and toxin of Verticillium dahliae[J]. Acta Botanica Sinica, 2003, 45(7): 802-808. [16] 吕金殿，甘　莉，阎龙飞． 棉花黄萎病菌毒素的纯化及特性研究[J]．植物病理学报, 1991, 21 (2): 129-133.       Lü Jin-dian, Gan Li, Yan Long-fei. Purified toxin of Verticillium dahliae from cotton and its properties[J]. Acta Phytopathologica Sinica, 1991, 21(2): 129-133. [17] WHITE P R. Potentially unlimited growth of excised tomato root tips in a liquid medium[J]. Plant Physiology, 1934, 9: 585- 600. [18] 甘　莉，吕金殿，汪沛洪． 棉花黄萎病菌分泌的糖蛋白毒素与其致病力的关系[J]．中国农业科学, 1995, 28(2): 58-65.        GAN Li, Lü Jin-dian, Wang Pei-hong. The relationship between glycoprotein toxin secreted from Verticillium dahliae of cotton and its pathogenicity[J]. Scientia Agricultura Sinica, 1995, 28(2): 58-65. [19] El-BEBANY A F, Rampitsch C, Daayf F. Proteomic analysis of the phytopathogenic soilborne fungus Verticillium dahliae reveals differential protein expression in isolates that differ in aggressiveness[J]. Proteomics, 2010, 10(2): 289-303. [20] ELLIS J G, Rafiqi M, Gan P, et al. Recent progress in discovery and functional analysis of effector proteins of fungal and oomycete plant pathogens[J]. Current Opinion in Plant Biology, 2009, 12(4): 399-405. [21] 彭　姗，吕学莲，高　峰，等．一种新的棉花黄、枯萎病快速接种方法的研究[J]．棉花学报, 2008, 20(3): 174-178.        PENG Shan, Lü Xue-lian, Gao Feng, et al. Study on a new rapid inoculation method for Verticillium wilt and Fusarium wilt of cotton[J]. Cotton Science, 2008, 20(3): 174-178. [22] 齐俊生，李怀方． 一种检测棉花黄萎菌毒素致萎性的新方法-叶片针刺涂抹法[J]．棉花学报, 2006, 18(4):  228-232.       QI Jun-sheng, Li Huai-fang. A new detection method of wilting induction by phytotoxin from Verticillium dahliae on cotton through leaf pricking and spreading [J]. Cotton Science, 2006, 18 (4): 228-232.  
PDF(382 KB)

文章所在专题

棉花

15

Accesses

0

Citation

Detail
段落导航
相关文章

/

〈 〉