Genome-Wide Identification and Expression Profile Analysis of DUF760 Gene Family in Rice
Qiang DU, Lingling HAN, Xiuwen XIAO, Jincheng LI, Mengyu SHEN, Zhilong WANG, Qiuhong CHEN
Genome-Wide Identification and Expression Profile Analysis of DUF760 Gene Family in Rice
To investigate the potential functions of DUF760s in rice growth and development,the genome-wide identification,classification,promoter sequence and expression profile analysis of the DUF760 gene family were performed.This research identified 6 and 8 members of DUF760 gene family in rice and Arabidopsis respectively by bioinformatics analysis.Phylogenetic tree analysis divided these members into two subfamilies,there were also some characteristic differences between the two subfamilies in protein conserved motif and gene structure.Multiple cis-acting elements responding to stresses and phytohormones existed in the promoter regions of rice DUF760 family genes.ABRE(abscisic acid response)element was present in the promoter sequence of all DUF760 family members,the promoter region of OsDUF760-1 possessed 9 abscisic acid(ABA)related response elements.The transcriptional expression level of OsDUF760-1 in rice was significantly down-regulated after ABA treatment,while OsDUF760-3 was significantly up-regulated.The expression change patterns of these two genes in rice after drought stress treatment were consistent with that after ABA treatment,which indicated that these two genes may participate in rice drought stress response through ABA signaling pathway,and play different roles.In addition to strong responses to ABA and drought stress treatments,members of rice DUF760 family also showed relatively strong expression changes in response to JA(Jasmonic acid),low temperature and M.oryzae treatments.
Rice / DUF760 gene family / Stress / Phytohormone / Expression profile analysis {{custom_keyword}} /
Tab.1 Primers used for relative expression level detection of OsDUF760 family members表1 OsDUF760家族成员相对表达量检测所用引物 |
| 基因名称 Gene name | 引物序列(5'—3') Primer sequence (5'—3') |
|---|---|
| OsDUF760-1 | F: CCGGCCAAGATAACTGTATTTG |
| R: CCAACAATAGTCAGGTTGCAAA | |
| OsDUF760-2 | F: CTACATCCGAGGTCTTGAGTAC |
| R: TCTTTCTGAGGAGAAATGCAGT | |
| OsDUF760-3 | F: AAACGAATTTCTCCTGGCTCTA |
| R: GAACTAACAGCTGCACATTAGG | |
| OsDUF760-4 | F: GCATGTATTGTTAGTCGCACAA |
| R: AAATGCATCCTCCACTAGAACA | |
| OsDUF760-5 | F: CTGATTCTTGCTCAGTCACAAG |
| R: GGATCAAAGCCCATCATGTAAC | |
| OsDUF760-6 | F: CAGCCCCTGATATCTATTCCTC |
| R: GGAGATCATGAATGTGCATCAC | |
| Ubiquitin | F: AACCAGCTGAGGCCCAAGA |
| R: ACGATTGATTTAACCAGTCCATGA |
Tab.2 Information of DUF760 family members in rice and arabidopsis表2 水稻和拟南芥DUF760家族成员信息 |
| 基因名称 Gene name | 基因序列号 Gene locus ID | 编码序列长度/bp CDS length | 氨基酸长度/aa Amino acid length | 等电点 pI | 分子量/ku MW |
|---|---|---|---|---|---|
| OsDUF760-1 | Os02g0833400 | 1 011 | 336 | 6.58 | 36.931 |
| OsDUF760-2 | Os03g0607500 | 1 218 | 405 | 4.94 | 44.787 |
| OsDUF760-3 | Os03g0717900 | 1 368 | 455 | 9.24 | 49.956 |
| OsDUF760-4 | Os04g0596300 | 1 218 | 405 | 5.51 | 45.306 |
| OsDUF760-5 | Os11g0456100 | 1 167 | 388 | 8.28 | 44.084 |
| OsDUF760-6 | Os12g0581700 | 1 182 | 393 | 5.10 | 43.185 |
| AtDUF760-1 | At1g32160 | 1 221 | 406 | 5.54 | 45.868 |
| AtDUF760-2 | At1g48450 | 1 272 | 423 | 5.86 | 47.189 |
| AtDUF760-3 | At1g63610 | 1 023 | 340 | 6.26 | 37.955 |
| AtDUF760-4 | At2g14910 | 1 161 | 386 | 5.04 | 43.396 |
| AtDUF760-5 | At3g07310 | 1 107 | 368 | 8.46 | 41.368 |
| AtDUF760-6 | At3g17800 | 1 266 | 421 | 9.20 | 46.067 |
| AtDUF760-7 | At5g14970 | 1 068 | 355 | 5.57 | 39.570 |
| AtDUF760-8 | At5g48590 | 1 035 | 344 | 7.67 | 38.959 |
Fig.1 Phylogenetic tree and motif analysis of DUF760 family members图1 DUF760家族成员的系统进化树和保守基序分析 |
Fig.6 Expression profile analysis of OsDUF760 family genes in response to abiotic stresses图6 OsDUF760家族基因的胁迫响应表达谱分析 |
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Collection(s)
Tab.1 Primers used for relative expression level detection of OsDUF760 family membersTab.2 Information of DUF760 family members in rice and arabidopsis
Fig.1 Phylogenetic tree and motif analysis of DUF760 family membersFig.2 Gene structure analysis of DUF760 family membersFig.3 Cis-acting elements identification in promoters of OsDUF760 family genesFig.4 Tissue expression profile analysis of OsDUF760 family genesFig.5 Expression profile analysis of OsDUF760 family genes in response to hormone treatmentsFig.6 Expression profile analysis of OsDUF760 family genes in response to abiotic stressesFig.7 Expression profile analysis of OsDUF760 family genes in response to biotic stress/
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