Expression Analysis of TNNI1 and TNNI3 in Striated Muscle of Maiwa Yak

Ya XIANG, Jincheng ZHONG, Zhijuan WU, Zhixin CHAI

Acta Agric Boreali Sin ›› 2022, Vol. 37 ›› Issue (6) : 210-218. DOI: 10.7668/hbnxb.20192913
Animal Husbandry·Fisheries·Veterinarian

Expression Analysis of TNNI1 and TNNI3 in Striated Muscle of Maiwa Yak

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Abstract

In order to explore the expression patterns of TNNI1 and TNNI3 in the striated muscle of Maiwa yak,this study selectd the gluteal muscle and myocardial tissue of Maiwa yak of different ages,morphological changes observed.The expression trend of TNNI1 gene in gluteal muscle tissue of Maiwa yak at different ages and its association with muscle fiber traits were carried out,the mRNA,protein differential expression and protein localization of TNNI1 and TNNI3 in myocardium were analyzed.The results showed that the muscle fiber diameter and density had certain characteristic changes with the increase of yak age.The muscle fiber diameter increased significantly with the increase of age,while the muscle fiber density had the opposite change.The mRNA expression of TNNI1 gene in gluteal muscle tissue of adult yaks was significantly higher than calves and weaned calves. TNNI1 mRNA expression in gluteus muscle was significantly positively correlated with muscle fiber diameter,and significantly negatively correlated with muscle fiber density.The mRNA expression level of TNNI1 in myocardium decreased with the increase of yak age,while the expression level of TNNI3 showed a trend of first increased and then decreased,and the mRNA expression level of TNNI1 was the highest in calf stage,while TNNI3 showed an upward trend with the increase of age.The expression levels of TNNI1 and TNNI3 proteins in myocardium decreased with the increase of yak age.The expression levels of TNNI1 and TNNI3 proteins in myocardium decreased with the increase of yak age.The TNNI3 mRNA expression decreased with the increase of yak age,which was consistent with the trend of protein expression.It provided basic data for exploring the regulation mechanism of TNNI1 and TNNI3 on yak muscle growth and development.TNNI1 and TNNI3 have significant regulatory effects on the growth and development of the muscle tissue of Maiwa yak at different ages,and the expression levels in the muscle tissue of yak at different ages are different.

Key words

Yak / TNNI1 / TNNI3 / Striated muscle / Western Blot / Immunohistochemistry

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Ya XIANG , Jincheng ZHONG , Zhijuan WU , Zhixin CHAI. Expression Analysis of TNNI1 and TNNI3 in Striated Muscle of Maiwa Yak. Acta Agriculturae Boreali-Sinica. 2022, 37(6): 210-218 https://doi.org/10.7668/hbnxb.20192913

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