刺芹侧耳交配型位点结构多态性分析

徐思佳, 李燕, 万佳宁, 杨瑞恒, 唐利华, 龚明, 鲍大鹏, 尚俊军

食用菌学报. 2021, 28(03): 19-26

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食用菌学报 ›› 2021, Vol. 28 ›› Issue (03) : 19-26. DOI: 10.16488/j.cnki.1005-9873.2021.03.003

刺芹侧耳交配型位点结构多态性分析

  • 徐思佳1,2#, 李燕2#, 万佳宁2, 杨瑞恒2, 唐利华2, 龚明2, 鲍大鹏1,2, 尚俊军1,2*
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Polymorphism Analysis of Mating-Type Loci in Pleurotus eryngii

  • XU Sijia1,2#, LI Yan2#, WAN Jianing2, YANG Ruiheng2, TANG Lihua2, GONG Ming2, BAO Dapeng1,2, SHANG Junjun1,2*
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摘要

对刺芹侧耳(Pleurotus eryngii)4个双核菌株的8个原生质体单核体进行基因组重测序和交配型位点的生物信息学分析,并对单核体之间的亲和性进行遗传分析。结果表明:8个单核体中含有3种A交配型位点和4种B交配型位点;其中3种A交配型位点都包含一对HD1和HD2基因,以典型的‘头对头'方式排列,HD基因两端有保守mip和β-fg基因;4种B交配型位点的信息素受体和信息素前体的数量及在基因组中的分布上差异较大,表明B交配型位点存在更丰富的遗传多样性。

Abstract

Protoplasts of eight monokaryons segregated from four dikarytotic Pleurotus eryngii strains were subjected to whole genome re-sequencing, bioinformatics analysis of their mating-type loci and genetic analysis of affinity between these monokaryons. The results showed that there were three A mating-type lociand four B mating-type loci in these monokaryons. All three A mating-type loci contain a pair of HD1 and HD2 genes arranged in a typical 'head-to-head' manner, and are closely flanked by conservative genes mip and β-fg at two ends, respectively. There are large variations in the number of pheromone receptor and pheromone precursor genes at the four B mating-type loci and their distribution in the genome, suggesting a high genetic diversity in B mating-typeloci.

关键词

刺芹侧耳 / 双核菌株 / 原生质体单核体 / 交配型位点

Key words

Pleurotus eryngii / dikaryotic strain / monokaryotic protoplast / mating-type locus

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徐思佳, 李燕, 万佳宁, 杨瑞恒, 唐利华, 龚明, 鲍大鹏, 尚俊军. 刺芹侧耳交配型位点结构多态性分析. 食用菌学报. 2021, 28(03): 19-26 https://doi.org/10.16488/j.cnki.1005-9873.2021.03.003
XU Sijia1,2#, LI Yan2#, WAN Jianing2, YANG Ruiheng2, TANG Lihua2, GONG Ming2, BAO Dapeng1,2, SHANG Junjun1,2*. Polymorphism Analysis of Mating-Type Loci in Pleurotus eryngii. Acta Edulis Fungi. 2021, 28(03): 19-26 https://doi.org/10.16488/j.cnki.1005-9873.2021.03.003

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